Team:PoznanSoft

From 2014.igem.org

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{{CSS/Main}}
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<h2 >MUFASA: Multiple fragments assembler for scarless cloning of big genetic constructs.</h2>
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<p>
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Overlap-based cloning techniques like Gibson Assembly and Circular Polimerase Extension Cloning (CPEC) have been recently becoming more and more popular. While available software allows rapid overlap design for constructs of conventional size, details of its functioning are not always fully transparent. MUFASA aims not only at facilitating the scarless cloning from the oligonucleotide design to putting the tubes into the thermocycler, but also at designing the fragments themselves in parallel with the overlaps for synthesis of big constructs de novo. Use of multiple overlaps pose a risk of non-specific hybridization and overlap “shadowing”. Such “shadowy places” may be eliminated by thermodynamic sequence optimization of both CPEC primers and fragments of the desired construct.
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<p style="color:#E7E7E7"> <a href="https://2014.igem.org/wiki/index.php?title=Team:PoznanSoft&action=edit"style="color:#FFFFFF"> Click here  to edit this page!</a> </p>
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<a href="https://2014.igem.org/Team:PoznanSoft"style="color:#000000">Home </a> </td>
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<a href="https://2014.igem.org/Team:PoznanSoft/Team"style="color:#000000"> Team </a> </td>
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<a href="https://igem.org/Team.cgi?year=2014&team_name=PoznanSoft"style="color:#000000"> Official Team Profile </a></td>
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<a href="https://2014.igem.org/Team:PoznanSoft/Project"style="color:#000000"> Project</a></td>
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<a href="https://2014.igem.org/Team:PoznanSoft/Parts"style="color:#000000"> Parts</a></td>
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<a href="https://2014.igem.org/Team:PoznanSoft/Modeling"style="color:#000000"> Modeling</a></td>
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<a href="https://2014.igem.org/Team:PoznanSoft/Notebook"style="color:#000000"> Notebook</a></td>
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<a href="https://2014.igem.org/Team:PoznanSoft/Safety"style=" color:#000000"> Safety </a></td>
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<a href="https://2014.igem.org/Team:PoznanSoft/Attributions"style="color:#000000"> Attributions </a></td>
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<td align ="center"> <a href="https://2014.igem.org/Main_Page"> <img src="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png" width="55px"></a> </td>
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<tr><td colspan="3"> <h3> Requirements </h3></td></tr>
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<p> Please be sure to keep these links, your audience will want to find your: </p>
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<!-- Links to other team pages -->
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<ul>
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<li><a href="https://2014.igem.org/Team:PoznanSoft">Home</a> </li>
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<li><a href="https://2014.igem.org/Team:PoznanSoft/Team">Team</a> </li>
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<li><a href="https://igem.org/Team.cgi?year=2013&team_name=PoznanSoft">Official Team Profile</a> </li>
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<li><a href="https://2014.igem.org/Team:PoznanSoft/Project">Project</a> </li>
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<li><a href="https://2014.igem.org/Team:PoznanSoft/Parts">Parts</a> </li>
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<li><a href="https://2014.igem.org/Team:PoznanSoft/Modeling">Modeling</a> </li>
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<li><a href="https://2014.igem.org/Team:PoznanSoft/Notebook">Notebook</a> </li>
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<li><a href="https://2014.igem.org/Team:PoznanSoft/Safety">Safety</a> </li>
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<li><a href="https://2014.igem.org/Team:PoznanSoft/Attributions">Attributions</a> </li>
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<td > </td>
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<p>There are a few wiki requirements teams must follow:</p>
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<li>All pages, images and files must be hosted on the <a href ="https://2014.igem.org/Special:Upload">  2014.igem.org server</a>. </li>
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<li>All pages must be created under the team’s name space.</li>
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<li>As part of your documentation, keep the links from the menu to the left. </li>
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<li>Do not use flash in wiki code. </li>
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<li>The <a href="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png"> iGEM logo </a> should be placed on the upper part of every page and should link to <a href="https://2014.igem.org/Main_Page">2014.igem.org</a>.</li>
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</ul>
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<p>Visit the <a href="https://2014.igem.org/Wiki_How-To"> Wiki How To page </a> for a complete list of requirements, tips and other useful information. </p>
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<tr><td colspan="3" > <h3> Tips  </h3></td></tr>
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<p>We are currently working on providing teams with some easy to use design templates.
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<br> In the meantime you can also view other team wikis for inspiration! Here are some very good examples</p>
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<li> <a href="https://2013.igem.org/Team:SDU-Denmark/"> 2013 SDU Denmark </a> </li>
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<li> <a href="https://2013.igem.org/Team:SYSU-China">2013 SYSU China</a> </li>
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<li> <a href="https://2013.igem.org/Team:Shenzhen_BGIC_ATCG"> 2013 Shenxhen BGIG ATCG </a></li>
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<li> <a href="https://2013.igem.org/Team:Colombia_Uniandes">2013 Colombia Unianades </a></li>
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<li> <a href="https://2013.igem.org/Team:Lethbridge">2013 Lethbridge</a></li>
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<p>For a full wiki list, you can visit <a href="https://igem.org/Team_Wikis?year=2013">iGEM 2013 web sites </a> and <a href="https://igem.org/Team_Wikis?year=2012">iGEM 2012 web sites</a>  lists. </p>
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<td> </td>
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<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
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<ul>
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<li>State your accomplishments! Tell people what you have achieved from the start. </li>
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<li>Be clear about what you are doing and what you plan to do.</li>
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<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
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<li>Make sure information is easy to find; nothing should be more than 3 clicks away.  </li>
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<li>Avoid using very small fonts and low contrast colors; information should be easy to read.  </li>
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<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="">iGEM 2013 calendar</a> </li>
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<li>Have lots of fun! </li>
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Revision as of 08:34, 16 October 2014

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