Team:Freiburg/Content/Results/Light system

From 2014.igem.org

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<h1>The light system</h1>
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<p>lights on</p>
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<h1>Results</h1>
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<li><a href ="/Team:Freiburg/Results/BioBrick">BioBrick</a></li><li>
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<a href ="/Team:Freiburg/Results/Receptor">Receptor characterisation</a></li><li>
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<a href ="/Team:Freiburg/Vector">Vector characterisation</a></li><li>
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<a href ="/Team:Freiburg/Light_system">Light-system characterisation</a></li><li>
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<a href ="/Team:Freiburg/Implementation">Implementation</a></li><li>
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<a href ="/Team:Freiburg/Summary">Summary</a></li><li style = "border-bottom: 4px solid #007A7A;">
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<p>One advantage of our system, the spacial resolution by infecting only distinct areas in a mammalian cell culture with our viral vector, was archived by the use of different light systems. We managed to induce the expression of the mCAT-1 receptor in non-murine cell lines by illumination with light of a special wave length. Since, the viral vector only infect cells that express the mCAT-1 receptor on their surfaces, we transferred genes into distinct areas of a otherwise homogenous tissue using this principle.
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<h2>Function of blue light system</h2>
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<h2>Function of red light system</h2>
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<h2>Light induced receptor/ blue light</h2>
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<h2>Receptor functionality</h2>
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<h2>SEAP as reporter</h2>
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<h2>MuLV SEAP</h2>
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Revision as of 20:58, 9 October 2014

The AcCELLerator

The light system

One advantage of our system, the spacial resolution by infecting only distinct areas in a mammalian cell culture with our viral vector, was archived by the use of different light systems. We managed to induce the expression of the mCAT-1 receptor in non-murine cell lines by illumination with light of a special wave length. Since, the viral vector only infect cells that express the mCAT-1 receptor on their surfaces, we transferred genes into distinct areas of a otherwise homogenous tissue using this principle.

Function of blue light system

Function of red light system

Light induced receptor/ blue light

Receptor functionality

SEAP as reporter

MuLV SEAP

Retrieved from "http://2014.igem.org/Team:Freiburg/Content/Results/Light_system"