Team:Paris Saclay/Notebook/September/1

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(Created page with "==B-Construction of the fusion protein== 'by Hoang Vu' We pricked out white colonies from Xgal-IPTG petri dish and blue colonies found in non Xgal-IPTG petri dish in a petri di...")
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==B-Construction of the fusion protein==
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====Lab Work====
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===B-Construction of the fusion protein===
'by Hoang Vu'
'by Hoang Vu'
We pricked out white colonies from Xgal-IPTG petri dish and blue colonies found in non Xgal-IPTG petri dish in a petri dish that contain Ampicillin to check if the blue ones are not came from a carrying of Xgal-IPTG during the precedent prick out.
We pricked out white colonies from Xgal-IPTG petri dish and blue colonies found in non Xgal-IPTG petri dish in a petri dish that contain Ampicillin to check if the blue ones are not came from a carrying of Xgal-IPTG during the precedent prick out.
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===D-Lemon scent===
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'' by melanie''
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Limone synthase
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We had a problem to clone LS in pGMETeasy and pPSI. So from the dish of streaking from [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/26#Streaking_of_colonies_transformed_by_BBa_K762100.2BpGEMTeasy_.28LS.29 26 August]We do PCR of all the clones:
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We use the [https://2014.igem.org/Team:Paris_Saclay/Protocols/PCR_for_bacterial_culture Protocol - PCR for bacterial culture] and we use the same PCR condition than on the [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/8#PCR August 8] but with the appropriate Primer and Biobrick.
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PPS5
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We check if we have the right insert in our plasmid pPS5 : CAD (cinnalyl alcool deshydrogenase)
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So we do a PCR in the same condition than [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/20#CAD_PCR August 20]
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PPS3 and PPS4
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We need to work on this plasmid tomorrow so we do some culture from the stock

Revision as of 18:11, 3 September 2014

Lab Work

B-Construction of the fusion protein

'by Hoang Vu'

We pricked out white colonies from Xgal-IPTG petri dish and blue colonies found in non Xgal-IPTG petri dish in a petri dish that contain Ampicillin to check if the blue ones are not came from a carrying of Xgal-IPTG during the precedent prick out.

D-Lemon scent

by melanie

Limone synthase

We had a problem to clone LS in pGMETeasy and pPSI. So from the dish of streaking from 26 AugustWe do PCR of all the clones:

We use the Protocol - PCR for bacterial culture and we use the same PCR condition than on the August 8 but with the appropriate Primer and Biobrick.


PPS5

We check if we have the right insert in our plasmid pPS5 : CAD (cinnalyl alcool deshydrogenase) So we do a PCR in the same condition than August 20


PPS3 and PPS4

We need to work on this plasmid tomorrow so we do some culture from the stock

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