Team:KAIT Japan/Results

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[[File:KAIT Japan 2014 iGEMRogo.png|center|link=https://2014.igem.org/Main_Page]]
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!align="center"|[[File:Kaitjapan protocol2.png|link=Team:KAIT_Japan/Protocol]]
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!align="center"|[[File:Kaitjapan notebook2.png|link=Team:KAIT_Japan/Notebook]]
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<H1>Results</H1>
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=<font size="7">Results</font>=
<br>
<br>
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<H1>GFP＋HlyA(BBa_K1414000) and HlyA＋GFP(BBA_K1414001)</H1>
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{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"
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| style="width:150px" |
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[[File:GFP.jpg]]
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::::::<font size=4> [Figure 1]</font>
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| style="border-left:3px dotted #99F" |
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=='''<font size="5">GFP</font>'''==
 +
<font size=4>
 +
We used "BBa_E0040".
 +
Length of "BBa_E0040"is 920bp.
 +
Following figure 1 is result of the GFP PCR.
 +
As a result,We were able to confirm a band about 920bp by GFP PCR.
 +
</font>
 +
|}
 +
<br>
 +
<br>
 +
 +
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"
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| style="width:150px" |
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[[File:HlyA.jpg]]
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::::::<font size=4> [Figure 2]</font>
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| style="border-left:3px dotted #99F" |
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=='''<font size="5">HlyA</font>'''==
 +
<font size=4>
 +
We used "BBa_K554002".
 +
Length of "BBa_K554002" is 186bp.
 +
Following figure 2 is result of the HlyA PCR.
 +
As a result,We were able to confirm a band about 186bp by HlyA PCR.
 +
</font>
 +
|}
 +
<br>
 +
<br>
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{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"
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| style="width:150px" |
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[[File:HlyA.jpg]]
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::::::<font size=4> [Figure 3]</font>
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[[File:GFP+HlyA.jpg]]
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::::::<font size=4> [Figure 4]</font>
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| style="border-left:3px dotted #99F" |
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=='''<font size="5">GFP＋HlyA and HlyA＋GFP</font>'''==
 +
<font size=4>
 +
GFP ligated with HlyA.Length of GFP＋HlyA is 920bp.
 +
Following figure 3 is GFP＋HlyA.
 +
Following figure 4 is HlyA＋GFP.
 +
We submitted these parts.
 +
But the function of these parts,we were not able to confirm it.
 +
Because we did not have time to experiment.
 +
 +
</font>
 +
|}
 +
 +
 +
 +
<H1>Creating parts of STAT3</H1>
 +
<font size="4">
 +
STAT3's sequence includes three recognition sequences of restriction enzyme.
 +
We try to mutant this sequence,and we could succeed to mutant about one place.
 +
</font>
 +
<br>
 +
<br>
 +
 +
 +
 +
<H1>Creating parts of IL-10α,IL-10β</H1>
 +
<font size="4">
 +
IL-10a's sequence includes six,IL-10b's sequence includes three recognition sequences of restriction enzyme.
 +
We try to mutant these sequences ,but we didn't succeed.
 +
</font>
 +
<br>
 +
<br>
 +
 +
 +
 +
<H1>Creating parts of HRV,IL-5Ra,IL-5Rb,AraC,Arac Promoter</H1>
 +
 +
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"
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| style="width:150px" |
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[[File:140927 Arac AracP HRV colonyPCR.jpg]]
 +
::::::<font size=4> [Figure 1]</font>
 +
[[File:140929 araC araC promoter TAvector colonyPCR.jpg]]
 +
::::::<font size=4> [Figure 2]</font>
 +
| style="border-left:3px dotted #99F" |
 +
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=='''<font size="5"> Result of colony PCR </font>'''==
 +
<font size=4>
 +
Figure 1 is colony PCR of colony transformed TA vector.
 +
Lane 1,2 are colony transformed TA vector inserted AraC gene.
 +
Lane 3,4 are colony transformed TA vector inserted AraC promoter gene.
 +
Lane 5,6,7 are colony transformed TA vector inserted HRV gene.
 +
The band of lane 5 was 325bp.
 +
Objective band size was 325bp.
 +
We are able to confirm that we get colony which has TA vecter inserted AraC promoter gene.
 +
<br>
 +
<br>
 +
Figure 2 is colony PCR of colony transformed TA vector inserted AraC gene.
 +
The band of lane 1,2 were 1104bp.
 +
Objective band size was 1149bp.
 +
We are able to confirm that we get colony which has TA vector inserted AraC gene.
 +
 +
</font>
 +
|}

Latest revision as of 09:49, 17 October 2014

ロゴ2.png

KAIT Japan2013 Kanako.png

KAIT Japan 2014 iGEMRogo.png
New home t.jpg

Home

New Team1.jpg

Team

New 考える人.jpg

Project

New 歯車.jpg

Parts

New １１１１１１１１１１１１１１１.jpg

Protocol

Ff.jpg

Notebook

New 結果.jpg

Results

New new 危険.jpg

Safety

New new ひと.jpg

Human Practice

Contents

Results


GFP＋HlyA(BBa_K1414000) and HlyA＋GFP(BBA_K1414001)

GFP.jpg

[Figure 1]

GFP

We used "BBa_E0040". Length of "BBa_E0040"is 920bp. Following figure 1 is result of the GFP PCR. As a result,We were able to confirm a band about 920bp by GFP PCR.



HlyA.jpg

[Figure 2]

HlyA

We used "BBa_K554002". Length of "BBa_K554002" is 186bp. Following figure 2 is result of the HlyA PCR. As a result,We were able to confirm a band about 186bp by HlyA PCR.



HlyA.jpg

[Figure 3]

GFP+HlyA.jpg

[Figure 4]

GFP＋HlyA and HlyA＋GFP

GFP ligated with HlyA.Length of GFP＋HlyA is 920bp. Following figure 3 is GFP＋HlyA. Following figure 4 is HlyA＋GFP. We submitted these parts. But the function of these parts,we were not able to confirm it. Because we did not have time to experiment.


Creating parts of STAT3

STAT3's sequence includes three recognition sequences of restriction enzyme. We try to mutant this sequence,and we could succeed to mutant about one place.


Creating parts of IL-10α,IL-10β

IL-10a's sequence includes six,IL-10b's sequence includes three recognition sequences of restriction enzyme. We try to mutant these sequences ,but we didn't succeed.


Creating parts of HRV,IL-5Ra,IL-5Rb,AraC,Arac Promoter

140927 Arac AracP HRV colonyPCR.jpg

[Figure 1]

140929 araC araC promoter TAvector colonyPCR.jpg

[Figure 2]

Result of colony PCR

Figure 1 is colony PCR of colony transformed TA vector. Lane 1,2 are colony transformed TA vector inserted AraC gene. Lane 3,4 are colony transformed TA vector inserted AraC promoter gene. Lane 5,6,7 are colony transformed TA vector inserted HRV gene. The band of lane 5 was 325bp. Objective band size was 325bp. We are able to confirm that we get colony which has TA vecter inserted AraC promoter gene.

Figure 2 is colony PCR of colony transformed TA vector inserted AraC gene. The band of lane 1,2 were 1104bp. Objective band size was 1149bp. We are able to confirm that we get colony which has TA vector inserted AraC gene.

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