Team:Yale

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<h1 >Biosynthesis of an Anti-biofouling Surface Binding Polymer with the 21st Amino Acid- L-Dopamine</h1>
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<h1 style="display:none">Biosynthesis of an Anti-biofouling Surface Binding Polymer with the 21st Amino Acid- L-DOPA</h1>
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<p style="color:#E7E7E7"> <a href="https://2014.igem.org/wiki/index.php?title=Team:Yale&action=edit"style="color:#FFFFFF"> Click here  to edit this page!</a> </p>
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<p> <img src="https://static.igem.org/mediawiki/2014/d/d7/Ampersand_for_Yale_iGEM-v2.png" width="1100"> <div id = "vidbutton"></div></p>
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<tr><td colspan="3"><div class = "well" style="margin-bottom:10px"><p> <strong><center>Producing a Novel Antimicrobial Surface-Binding Peptide Using an Improved T7 Expression System</center></strong><p>
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Biofilm formation on surfaces is an issue in the medical field, naval industry, and other areas. We developed an anti-fouling peptide with two modular components: a mussel adhesion protein (MAP) anchor and LL-37, an antimicrobial peptide. MAPs can selectively attach to metal and organic surfaces via L-3,5-dihydroxyphenylalanine (L-DOPA), a nonstandard amino acid that was incorporated using a genetically recoded organism (GRO). Because this peptide is toxic to the GRO in which it is produced, we designed a better controlled inducible system that limits basal expression. This was achieved through a novel T7 riboregulation system that controls expression at both the transcriptional and translational levels.  This improved system is a precise synthetic switch for the expression of cytotoxic substances in the already robust T7 system. Lastly, the antimicrobial surface-binding peptide was assayed for functionality.  
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<a href="https://2014.igem.org/Team:Yale"style="color:#000000">Home </a> </td>
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<a href="https://2014.igem.org/Team:Yale/Team"style="color:#000000"> Team </a> </td>
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<a href="https://igem.org/Team.cgi?year=2014&team_name=Yale"style="color:#000000"> Official Team Profile </a></td>
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<a href="https://2014.igem.org/Team:Yale/Project"style="color:#000000"> Project</a></td>
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<a href="https://2014.igem.org/Team:Yale/Modeling"style="color:#000000"> Modeling</a></td>
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<a href="https://2014.igem.org/Team:Yale/Safety"style=" color:#000000"> Safety </a></td>
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<a href="https://2014.igem.org/Team:Yale/Attributions"style="color:#000000"> Attributions </a></td>
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<p> Please be sure to keep these links, your audience will want to find your: </p>
 
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<li><a href="https://2014.igem.org/Team:Yale">Home</a> </li>
 
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<li><a href="https://2014.igem.org/Team:Yale/Team">Team</a> </li>
 
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<li><a href="https://2014.igem.org/Team:Yale/Project">Project</a> </li>
 
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<li><a href="https://2014.igem.org/Team:Yale/Parts">Parts</a> </li>
 
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<li><a href="https://2014.igem.org/Team:Yale/Modeling">Modeling</a> </li>
 
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<li><a href="https://2014.igem.org/Team:Yale/Safety">Safety</a> </li>
 
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<li>The <a href="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png"> iGEM logo </a> should be placed on the upper part of every page and should link to <a href="https://2014.igem.org/Main_Page">2014.igem.org</a>.</li>
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<p>Visit the <a href="https://2014.igem.org/Wiki_How-To"> Wiki How To page </a> for a complete list of requirements, tips and other useful information. </p>
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<p>We are currently working on providing teams with some easy to use design templates.
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<br> In the meantime you can also view other team wikis for inspiration! Here are some very good examples</p>
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<li> <a href="https://2013.igem.org/Team:SDU-Denmark/"> 2013 SDU Denmark </a> </li>
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<li> <a href="https://2013.igem.org/Team:Shenzhen_BGIC_ATCG"> 2013 Shenxhen BGIG ATCG </a></li>
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<li> <a href="https://2013.igem.org/Team:Colombia_Uniandes">2013 Colombia Unianades </a></li>
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<p>For a full wiki list, you can visit <a href="https://igem.org/Team_Wikis?year=2013">iGEM 2013 web sites </a> and <a href="https://igem.org/Team_Wikis?year=2012">iGEM 2012 web sites</a>  lists. </p>
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<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="">iGEM 2013 calendar</a> </li>
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<li>Have lots of fun! </li>
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Latest revision as of 03:37, 18 October 2014

Biosynthesis of an Anti-biofouling Surface Binding Polymer with the 21st Amino Acid- L-DOPA

Producing a Novel Antimicrobial Surface-Binding Peptide Using an Improved T7 Expression System

Biofilm formation on surfaces is an issue in the medical field, naval industry, and other areas. We developed an anti-fouling peptide with two modular components: a mussel adhesion protein (MAP) anchor and LL-37, an antimicrobial peptide. MAPs can selectively attach to metal and organic surfaces via L-3,5-dihydroxyphenylalanine (L-DOPA), a nonstandard amino acid that was incorporated using a genetically recoded organism (GRO). Because this peptide is toxic to the GRO in which it is produced, we designed a better controlled inducible system that limits basal expression. This was achieved through a novel T7 riboregulation system that controls expression at both the transcriptional and translational levels. This improved system is a precise synthetic switch for the expression of cytotoxic substances in the already robust T7 system. Lastly, the antimicrobial surface-binding peptide was assayed for functionality.

Main Campus:
Molecular, Cellular & Developmental Biology
219 Prospect Street
P.O. Box 208103
New Haven, CT 06520
Phone: 203.432.3783
igem@yale.edu
natalie.ma@yale.edu (Graduate Advisor)
Copyright (c) 2014 Yale IGEM

Retrieved from "http://2014.igem.org/Team:Yale"