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| - | {{CSS/Site}} | + | {{ITESM14/Style}} |
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| | <html> | | <html> |
| - | <head>
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| - | <!-- Latest compiled and minified CSS -->
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| - | <link rel="stylesheet" href="https://maxcdn.bootstrapcdn.com/bootstrap/3.2.0/css/bootstrap.min.css">
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| | | | |
| - | <!-- Latest compiled and minified JavaScript -->
| + | <div id="wiki-body"> |
| - | <script src="https://ajax.googleapis.com/ajax/libs/jquery/1.11.1/jquery.min.js"></script>
| + | |
| - | <script src="https://maxcdn.bootstrapcdn.com/bootstrap/3.2.0/js/bootstrap.min.js"></script>
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| - | <link href='http://fonts.googleapis.com/css?family=Yanone+Kaffeesatz:400,200,300,700&subset=latin,latin-ext' rel='stylesheet' type='text/css'> | + | <body> |
| - | </head> | + | |
| | + | <header id="wiki-header" class="initial-nav-scroll-up"> |
| | + | </header> |
| | + | |
| | + | <div id ="wiki-content" class="main-content"> |
| | | | |
| - | <body>
| + | <div class=" jumbotron" style=" background: transparent; padding-top: 0; margin-bottom: 0; padding-bottom: 0; padding-left: 0;"> |
| - | <div class="mypic navbar-fixed-top col-md-6" style="padding-left: 0px"> | + | <div class="container style="margin-left:20%;"> |
| - | <img src="igem_esquina-01.png"></img> | + | <span><img class="col-sm-8 col-sm-offset-2 img img-responsive" style="padding:0px 0px 0px 0px; margin-left:10%; -moz-transform: scaleX(-1);-o-transform: scaleX(-1);-webkit-transform: scaleX(-1);transform: scaleX(-1);filter: FlipH; |
| - | </div> | + | -ms-filter: "FlipH";" src="https://static.igem.org/mediawiki/2014/3/37/ITESM14_Home.png"></span> |
| - | <div class="navbar navbar-default navbar-fixed-top" role="navigation"> | + | <span><h1 class="col-md-10 text-center"style="color: #f4ff3b; font-size: 5vw; padding: 0; margin: 0 0 30px 10%; font-weight: lighter;"><i>"Using a Trojan horse strategy to kill cancerous cells"</i></h1></span> |
| - | <div class="container">
| + | |
| - | <div class="navbar-header">
| + | |
| - | <button type="button" class="navbar-toggle collapsed" data-toggle="collapse" data-target=".navbar-collapse">
| + | |
| - | <span class="sr-only">Toggle navigation</span>
| + | |
| - | <span class="icon-bar"></span>
| + | |
| - | <span class="icon-bar"></span>
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| - | <span class="icon-bar"></span>
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| - | </button>
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| | </div> | | </div> |
| - | <div class="collapse navbar-collapse pull-right">
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| - | <ul class="nav navbar-nav" style="font-family: 'Yanone Kaffeesatz'; font-size: 150%;">
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| - | <li class="active"><a href="#">Project</a></li>
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| - | <li><a href="achievements.html">Achievements</a></li>
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| - | <li><a href="#contact">Safety</a></li>
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| - | <li><a href="#contact">Human Practice</a></li>
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| - | <li><a href="#contact">Collaboration</a></li>
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| - | <li><a href="#contact">Team</a></li>
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| - | </ul>
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| - | </div><!--/.nav-collapse -->
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| | </div> | | </div> |
| - | </div>
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| | | | |
| - | <div id ="content" class="container main-content">
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| | <div class="well"> | | <div class="well"> |
| - | <h1>Video</h1> | + | <div class="container"> |
| | + | <div class="video-container"> |
| | + | <video width="100%" controls poster="https://static.igem.org/mediawiki/2014/9/93/Poster_video_team.png"> |
| | + | <source src="https://static.igem.org/mediawiki/2014/8/88/ITESM14_videoprincipal.mov" type="video/mp4"> |
| | + | Your browser does not support the video tag. |
| | + | </video> |
| | + | </div> |
| | <hr> | | <hr> |
| - | <h1>Proyect Description</h1> | + | |
| | + | <h1>Project Description</h1> |
| | <p> | | <p> |
| | By harnessing the inherent ability of facultative anaerobic bacteria to colonize and grow in tumoral environments, this project aims to develop a bacterial cancer therapy. A genetically modified <em>E. coli</em> strain will have knockouts in the <em>lpp</em> and <em>msbB</em> genes, which encode for the Braun's lipoprotein and for the myristic acid moiety transporter of the lipopolysaccharide, respectively. These genes are known to trigger an immune response in the human body; by deleting them, the impact originating from a bacterial intravenous administration will be reduced. Furthermore, these bacteria will produce M13-modified bacteriophages under the control of a quorum sensing system. The resulting phages will be capable of binding to the GRP78 receptor, which is usually overexpressed in cancerous cells. Subsequently, they will internalize and transfect the cancerous cells with two different genes: apoptin, responsible for an apoptotic protein specific for cancerous cells which will be regulated by a constitutive promoter and a survivin siRNA. The latter will inhibit the uncontrollable growth characteristic of cancer cells, making them more vulnerable to apoptosis. </b> | | By harnessing the inherent ability of facultative anaerobic bacteria to colonize and grow in tumoral environments, this project aims to develop a bacterial cancer therapy. A genetically modified <em>E. coli</em> strain will have knockouts in the <em>lpp</em> and <em>msbB</em> genes, which encode for the Braun's lipoprotein and for the myristic acid moiety transporter of the lipopolysaccharide, respectively. These genes are known to trigger an immune response in the human body; by deleting them, the impact originating from a bacterial intravenous administration will be reduced. Furthermore, these bacteria will produce M13-modified bacteriophages under the control of a quorum sensing system. The resulting phages will be capable of binding to the GRP78 receptor, which is usually overexpressed in cancerous cells. Subsequently, they will internalize and transfect the cancerous cells with two different genes: apoptin, responsible for an apoptotic protein specific for cancerous cells which will be regulated by a constitutive promoter and a survivin siRNA. The latter will inhibit the uncontrollable growth characteristic of cancer cells, making them more vulnerable to apoptosis. </b> |
| | </p> | | </p> |
| | <hr> | | <hr> |
| | + | <div id="modulos" class="centered"> |
| | + | <div class="col-md-3 col-sm-3 col-xs-6"> |
| | + | <a href="https://2014.igem.org/Team:Tec-Monterrey/ITESM14_project.html#tab_module1"> |
| | + | <img class="img img-responsive" src="https://static.igem.org/mediawiki/2014/2/21/ITESM14_module1-banner.png"> |
| | + | <h1 class="col-md-10 text-center" class="module-title">Attenuation</h1> |
| | + | <span class="texto-modulos col-md-10 text-center"> |
| | + | <p class="module-description">The module talks about the a knockout of two genes of the E. coli endotoxins to attenuate the immunological response of the patients.</p> |
| | + | </span> |
| | + | </a> |
| | + | </div> |
| | + | <div class="col-md-3 col-sm-3 col-xs-6"> |
| | + | <a href="https://2014.igem.org/Team:Tec-Monterrey/ITESM14_project.html#tab_module2"> |
| | + | <img class="img img-responsive" src="https://static.igem.org/mediawiki/2014/1/11/ITESM14_module2-banner.png"> |
| | + | <h1 class="col-md-10 col-sm-10 col-xs-10 text-center" class="module-title">Phage engineering</h1> |
| | + | <span class="texto-modulos col-md-10 text-center"> |
| | + | <p class="module-description">Modifications were made in the phage M13, so it could infect cancerous cells and be produced using Quorum sensing.</p> |
| | + | </span> |
| | + | </a> |
| | + | </div> |
| | + | <div class="col-md-3 col-sm-3 col-xs-6"> |
| | + | <a href="https://2014.igem.org/Team:Tec-Monterrey/ITESM14_project.html#tab_module3"> |
| | + | <img class="img img-responsive" src="https://static.igem.org/mediawiki/2014/d/d0/ITESM14_module3-banner.png"> |
| | + | <h1 class="col-md-10 col-sm-10 col-xs-10 text-center" class="module-title">Quorum sensing</h1> |
| | + | <span class="texto-modulos col-md-10 text-center"> |
| | + | <p class="module-description">This system was implemented in the knock-out and is used to cause a coordinated attack from the bacteria that infects the phage.</p> |
| | + | </span> |
| | + | </a> |
| | + | </div> |
| | + | <div class="col-md-3 col-sm-3 col-xs-6"> |
| | + | <a href="https://2014.igem.org/Team:Tec-Monterrey/ITESM14_project.html#tab_module4"> |
| | + | <img class="img img-responsive" src="https://static.igem.org/mediawiki/2014/c/c1/ITESM14_module4-banner.png"> |
| | + | <h1 class="col-md-10 col-sm-10 col-xs-10 text-center" class="module-title">Effectors</h1> |
| | + | <span class="texto-modulos col-md-10 text-center"> |
| | + | <p class="module-description">The action of our effectors, apoptin and siRNA, against cancerous cells is explained here.</p> |
| | + | </span> |
| | + | </a> |
| | + | </div> |
| | + | </div> |
| | + | </div> |
| | + | </div> |
| | + | </div> |
| | | | |
| - | <!-- Old content start -->
| + | </body> |
| - | <div>
| + | |
| - | <!--navigation menu -->
| + | |
| - | <nav class="collapse navbar-collapse bs-navbar-collapse" role="navigation">
| + | |
| - | <td align="center" colspan="3">
| + | |
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| - | <table width="100%"> | + | <footer id="wiki-footer" > |
| - | <tr heigth="15px"></tr>
| + | </footer> |
| - | <tr heigth="75px">
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| | + | <script> |
| | + | $(window).load(function() { |
| | + | $( "#home" ).addClass("active"); |
| | + | }); |
| | + | </script> |
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| - | <td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
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| - | <a href="https://2014.igem.org/Team:Tec-Monterrey"style="color:#000000">Home </a> </td>
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| - | <td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
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| - | <a href="https://2014.igem.org/Team:Tec-Monterrey/Team"style="color:#000000"> Team </a> </td>
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| - | <td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
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| - | <a href="https://igem.org/Team.cgi?year=2014&team_name=Tec-Monterrey"style="color:#000000"> Official Team Profile </a></td>
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| - | <td style="border:1px solid black" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
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| - | <a href="https://2014.igem.org/Team:Tec-Monterrey/Project"style="color:#000000"> Project</a></td>
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| - | <td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
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| - | <a href="https://2014.igem.org/Team:Tec-Monterrey/Parts"style="color:#000000"> Parts</a></td>
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| - | <td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
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| - | <a href="https://2014.igem.org/Team:Tec-Monterrey/Modeling"style="color:#000000"> Modeling</a></td>
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| - | <td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
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| - | <a href="https://2014.igem.org/Team:Tec-Monterrey/Notebook"style="color:#000000"> Notebook</a></td>
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| - | <td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
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| - | <a href="https://2014.igem.org/Team:Tec-Monterrey/Safety"style=" color:#000000"> Safety </a></td>
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| - | <td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
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| - | <a href="https://2014.igem.org/Team:Tec-Monterrey/Attributions"style="color:#000000"> Attributions </a></td>
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| - | <td align ="center"> <a href="https://2014.igem.org/Main_Page"> <img src="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png" width="55px"></a> </td>
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| - | </tr>
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| - | </table>
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| - | </nav>
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| - | <tr> <td colspan="3" height="15px"> </td></tr>
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| - | <tr><td bgColor="#e7e7e7" colspan="3" height="1px"> </tr>
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| - | <tr> <td colspan="3" height="5px"> </td></tr>
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| - | <br>
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| - | <br>
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| - | <!--requirements section -->
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| - | <tr><td colspan="3"> <h3> Requirements </h3></td></tr>
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| - | <tr>
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| - | <td width="45%" valign="top">
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| - |
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| - | <p> Please be sure to keep these links, your audience will want to find your: </p>
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| - |
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| - | <!-- Links to other team pages -->
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| - | <ul>
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| - | <li><a href="https://2014.igem.org/Team:Tec-Monterrey">Home</a> </li>
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| - | <li><a href="https://2014.igem.org/Team:Tec-Monterrey/Team">Team</a> </li>
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| - | <li><a href="https://igem.org/Team.cgi?year=2013&team_name=Tec-Monterrey">Official Team Profile</a> </li>
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| - | <li><a href="https://2014.igem.org/Team:Tec-Monterrey/Project">Project</a> </li>
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| - | <li><a href="https://2014.igem.org/Team:Tec-Monterrey/Parts">Parts</a> </li>
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| - | <li><a href="https://2014.igem.org/Team:Tec-Monterrey/Modeling">Modeling</a> </li>
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| - | <li><a href="https://2014.igem.org/Team:Tec-Monterrey/Notebook">Notebook</a> </li>
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| - | <li><a href="https://2014.igem.org/Team:Tec-Monterrey/Safety">Safety</a> </li>
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| - | <li><a href="https://2014.igem.org/Team:Tec-Monterrey/Attributions">Attributions</a> </li>
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| - | <td > </td>
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| - | <td width="45%">
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| - | <p>There are a few wiki requirements teams must follow:</p>
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| - | <ul>
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| - | <li>All pages, images and files must be hosted on the <a href ="https://2014.igem.org/Special:Upload"> 2014.igem.org server</a>. </li>
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| - | <li>All pages must be created under the team’s name space.</li>
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| - | <li>As part of your documentation, keep the links from the menu to the left. </li>
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| - | <li>Do not use flash in wiki code. </li>
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| - | <li>The <a href="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png"> iGEM logo </a> should be placed on the upper part of every page and should link to <a href="https://2014.igem.org/Main_Page">2014.igem.org</a>.</li>
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| - | </ul>
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| - | <p>Visit the <a href="https://2014.igem.org/Wiki_How-To"> Wiki How To page </a> for a complete list of requirements, tips and other useful information. </p>
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| - | <tr> <td colspan="3" height="15px"> </td></tr>
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| - | <tr><td bgColor="#e7e7e7" colspan="3" height="1"> </tr>
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| - | <!--tips -->
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| - | <tr><td colspan="3" > <h3> Tips </h3></td></tr>
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| - | <td width="45%" valign="top">
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| - | <p>We are currently working on providing teams with some easy to use design templates.
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| - | <br> In the meantime you can also view other team wikis for inspiration! Here are some very good examples</p>
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| - | <ul>
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| - | <li> <a href="https://2013.igem.org/Team:SDU-Denmark/"> 2013 SDU Denmark </a> </li>
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| - | <li> <a href="https://2013.igem.org/Team:SYSU-China">2013 SYSU China</a> </li>
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| - | <li> <a href="https://2013.igem.org/Team:Shenzhen_BGIC_ATCG"> 2013 Shenxhen BGIG ATCG </a></li>
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| - | <li> <a href="https://2013.igem.org/Team:Colombia_Uniandes">2013 Colombia Unianades </a></li>
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| - | <li> <a href="https://2013.igem.org/Team:Lethbridge">2013 Lethbridge</a></li>
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| - | </ul>
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| - | <p>For a full wiki list, you can visit <a href="https://igem.org/Team_Wikis?year=2013">iGEM 2013 web sites </a> and <a href="https://igem.org/Team_Wikis?year=2012">iGEM 2012 web sites</a> lists. </p>
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| - | <td> </td>
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| - | <td width="45%">
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| - | <p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
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| - | <ul>
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| - | <li>State your accomplishments! Tell people what you have achieved from the start. </li>
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| - | <li>Be clear about what you are doing and what you plan to do.</li>
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| - | <li>You have a global audience! Consider the different backgrounds that your users come from.</li>
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| - | <li>Make sure information is easy to find; nothing should be more than 3 clicks away. </li>
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| - | <li>Avoid using very small fonts and low contrast colors; information should be easy to read. </li>
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| - | <li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="">iGEM 2013 calendar</a> </li>
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| - | <li>Have lots of fun! </li>
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| - | </ul>
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| - | </br>
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| - | </td>
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| - | </table>
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| - | </div>
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| - | <!--Old content end -->
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| - | <h1>Modules</h1>
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| - | <p>
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| - | Lorem ipsum dolor sit amet, consectetur adipiscing elit. Phasellus commodo ante id mauris scelerisque ullamcorper. Praesent risus enim, tincidunt quis est sed, aliquet sagittis velit. Maecenas purus eros, maximus ac dignissim vel, venenatis iaculis sem. Praesent vel pellentesque eros. Pellentesque eget dictum dolor. Quisque facilisis turpis vitae felis feugiat elementum. Morbi in velit diam. Sed imperdiet dolor ac quam pulvinar, eu pellentesque libero laoreet. Mauris maximus, purus in ultrices dapibus, libero ante vehicula leo, a pharetra lacus leo faucibus purus. Interdum et malesuada fames ac ante ipsum primis in faucibus. Suspendisse potenti. Cras pellentesque nunc est, a aliquet dui fermentum sit amet.
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| - | Nam volutpat vel leo quis rhoncus. Pellentesque orci odio, dignissim eget auctor sodales, lobortis sit amet metus. Integer in tortor et erat vehicula condimentum ac a mi. Nulla ac iaculis nibh. Morbi bibendum pretium quam, at consectetur diam. Pellentesque in turpis nec sem varius sagittis in at eros. Maecenas euismod orci sed ultrices dignissim. Phasellus vitae elit imperdiet, tristique lectus convallis, vulputate sapien. Integer vel arcu lobortis, semper lacus eu, porta eros. Ut elementum pharetra placerat. In in felis gravida, ultrices leo sed, ultricies mauris.
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| - | Fusce purus felis, tincidunt non diam a, hendrerit viverra dolor. Suspendisse condimentum metus molestie dolor sagittis faucibus. Sed ut mauris aliquam orci luctus maximus. Curabitur luctus tempus quam, id ultricies neque tristique vitae. Mauris gravida vulputate nisi vel luctus. Aliquam iaculis aliquet tortor in pulvinar. Integer facilisis sit amet sapien at egestas. In gravida mi lectus, nec facilisis mi dapibus ac. Praesent quis viverra velit, sed ullamcorper neque. Fusce volutpat nulla diam, ut scelerisque purus egestas vitae. Suspendisse aliquet diam et est accumsan, in feugiat nisi sodales. Ut convallis, enim in eleifend porta, urna dui sodales orci, sit amet hendrerit orci nunc ac elit. Duis faucibus sodales purus. Morbi vitae arcu leo. Mauris egestas ultricies augue, at viverra mi vulputate ac. Suspendisse nec tincidunt justo.
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| - | Maecenas rhoncus mauris id ipsum sodales, ut dictum nisl rutrum. Aliquam commodo est dolor, vel cursus risus accumsan in. Duis sed lectus eu quam bibendum scelerisque sit amet iaculis metus. Aliquam et ullamcorper nisl, non vestibulum lacus. Nulla suscipit urna enim, et convallis tellus laoreet vel. Sed iaculis odio at massa semper finibus. In accumsan volutpat lectus id faucibus. Nam id consequat arcu. Praesent semper blandit mi, non lobortis velit dignissim quis. In non orci vehicula ligula accumsan vehicula. Morbi vehicula et justo eget tincidunt. Integer vulputate nulla sed suscipit pulvinar. Phasellus nibh turpis, interdum eu purus porta, lacinia ornare eros. Integer consequat metus libero. Proin pharetra eros vel orci dapibus convallis. Mauris condimentum dictum felis, at bibendum lacus dignissim in.
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| - | Cras semper ipsum ac nunc commodo faucibus. Vestibulum quis justo ullamcorper tortor auctor mattis. Nulla ac tellus vitae ipsum eleifend interdum. Aliquam condimentum dui est, a mattis lorem convallis ut. Sed a nisl aliquam, porta neque non, ultricies erat. Nunc ultrices dolor orci, ut pretium velit interdum ac. Integer enim nulla, consectetur a ipsum a, laoreet efficitur eros. In enim nulla, dignissim eu nisl ac, porta sagittis metus. Nam vulputate, risus sit amet consectetur congue, risus odio ultrices neque, a ultrices magna sem ac dui. Nullam sapien velit, suscipit non vulputate id, aliquam sed sapien. Duis et ullamcorper sem.
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| - | Lorem ipsum dolor sit amet, consectetur adipiscing elit. Phasellus commodo ante id mauris scelerisque ullamcorper. Praesent risus enim, tincidunt quis est sed, aliquet sagittis velit. Maecenas purus eros, maximus ac dignissim vel, venenatis iaculis sem. Praesent vel pellentesque eros. Pellentesque eget dictum dolor. Quisque facilisis turpis vitae felis feugiat elementum. Morbi in velit diam. Sed imperdiet dolor ac quam pulvinar, eu pellentesque libero laoreet. Mauris maximus, purus in ultrices dapibus, libero ante vehicula leo, a pharetra lacus leo faucibus purus. Interdum et malesuada fames ac ante ipsum primis in faucibus. Suspendisse potenti. Cras pellentesque nunc est, a aliquet dui fermentum sit amet.
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| - | Nam volutpat vel leo quis rhoncus. Pellentesque orci odio, dignissim eget auctor sodales, lobortis sit amet metus. Integer in tortor et erat vehicula condimentum ac a mi. Nulla ac iaculis nibh. Morbi bibendum pretium quam, at consectetur diam. Pellentesque in turpis nec sem varius sagittis in at eros. Maecenas euismod orci sed ultrices dignissim. Phasellus vitae elit imperdiet, tristique lectus convallis, vulputate sapien. Integer vel arcu lobortis, semper lacus eu, porta eros. Ut elementum pharetra placerat. In in felis gravida, ultrices leo sed, ultricies mauris.
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| - | Fusce purus felis, tincidunt non diam a, hendrerit viverra dolor. Suspendisse condimentum metus molestie dolor sagittis faucibus. Sed ut mauris aliquam orci luctus maximus. Curabitur luctus tempus quam, id ultricies neque tristique vitae. Mauris gravida vulputate nisi vel luctus. Aliquam iaculis aliquet tortor in pulvinar. Integer facilisis sit amet sapien at egestas. In gravida mi lectus, nec facilisis mi dapibus ac. Praesent quis viverra velit, sed ullamcorper neque. Fusce volutpat nulla diam, ut scelerisque purus egestas vitae. Suspendisse aliquet diam et est accumsan, in feugiat nisi sodales. Ut convallis, enim in eleifend porta, urna dui sodales orci, sit amet hendrerit orci nunc ac elit. Duis faucibus sodales purus. Morbi vitae arcu leo. Mauris egestas ultricies augue, at viverra mi vulputate ac. Suspendisse nec tincidunt justo.
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| - | Maecenas rhoncus mauris id ipsum sodales, ut dictum nisl rutrum. Aliquam commodo est dolor, vel cursus risus accumsan in. Duis sed lectus eu quam bibendum scelerisque sit amet iaculis metus. Aliquam et ullamcorper nisl, non vestibulum lacus. Nulla suscipit urna enim, et convallis tellus laoreet vel. Sed iaculis odio at massa semper finibus. In accumsan volutpat lectus id faucibus. Nam id consequat arcu. Praesent semper blandit mi, non lobortis velit dignissim quis. In non orci vehicula ligula accumsan vehicula. Morbi vehicula et justo eget tincidunt. Integer vulputate nulla sed suscipit pulvinar. Phasellus nibh turpis, interdum eu purus porta, lacinia ornare eros. Integer consequat metus libero. Proin pharetra eros vel orci dapibus convallis. Mauris condimentum dictum felis, at bibendum lacus dignissim in.
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| - | Cras semper ipsum ac nunc commodo faucibus. Vestibulum quis justo ullamcorper tortor auctor mattis. Nulla ac tellus vitae ipsum eleifend interdum. Aliquam condimentum dui est, a mattis lorem convallis ut. Sed a nisl aliquam, porta neque non, ultricies erat. Nunc ultrices dolor orci, ut pretium velit interdum ac. Integer enim nulla, consectetur a ipsum a, laoreet efficitur eros. In enim nulla, dignissim eu nisl ac, porta sagittis metus. Nam vulputate, risus sit amet consectetur congue, risus odio ultrices neque, a ultrices magna sem ac dui. Nullam sapien velit, suscipit non vulputate id, aliquam sed sapien. Duis et ullamcorper sem.
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| - | Lorem ipsum dolor sit amet, consectetur adipiscing elit. Phasellus commodo ante id mauris scelerisque ullamcorper. Praesent risus enim, tincidunt quis est sed, aliquet sagittis velit. Maecenas purus eros, maximus ac dignissim vel, venenatis iaculis sem. Praesent vel pellentesque eros. Pellentesque eget dictum dolor. Quisque facilisis turpis vitae felis feugiat elementum. Morbi in velit diam. Sed imperdiet dolor ac quam pulvinar, eu pellentesque libero laoreet. Mauris maximus, purus in ultrices dapibus, libero ante vehicula leo, a pharetra lacus leo faucibus purus. Interdum et malesuada fames ac ante ipsum primis in faucibus. Suspendisse potenti. Cras pellentesque nunc est, a aliquet dui fermentum sit amet.
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| - | Nam volutpat vel leo quis rhoncus. Pellentesque orci odio, dignissim eget auctor sodales, lobortis sit amet metus. Integer in tortor et erat vehicula condimentum ac a mi. Nulla ac iaculis nibh. Morbi bibendum pretium quam, at consectetur diam. Pellentesque in turpis nec sem varius sagittis in at eros. Maecenas euismod orci sed ultrices dignissim. Phasellus vitae elit imperdiet, tristique lectus convallis, vulputate sapien. Integer vel arcu lobortis, semper lacus eu, porta eros. Ut elementum pharetra placerat. In in felis gravida, ultrices leo sed, ultricies mauris.
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| - | Fusce purus felis, tincidunt non diam a, hendrerit viverra dolor. Suspendisse condimentum metus molestie dolor sagittis faucibus. Sed ut mauris aliquam orci luctus maximus. Curabitur luctus tempus quam, id ultricies neque tristique vitae. Mauris gravida vulputate nisi vel luctus. Aliquam iaculis aliquet tortor in pulvinar. Integer facilisis sit amet sapien at egestas. In gravida mi lectus, nec facilisis mi dapibus ac. Praesent quis viverra velit, sed ullamcorper neque. Fusce volutpat nulla diam, ut scelerisque purus egestas vitae. Suspendisse aliquet diam et est accumsan, in feugiat nisi sodales. Ut convallis, enim in eleifend porta, urna dui sodales orci, sit amet hendrerit orci nunc ac elit. Duis faucibus sodales purus. Morbi vitae arcu leo. Mauris egestas ultricies augue, at viverra mi vulputate ac. Suspendisse nec tincidunt justo.
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| - | Maecenas rhoncus mauris id ipsum sodales, ut dictum nisl rutrum. Aliquam commodo est dolor, vel cursus risus accumsan in. Duis sed lectus eu quam bibendum scelerisque sit amet iaculis metus. Aliquam et ullamcorper nisl, non vestibulum lacus. Nulla suscipit urna enim, et convallis tellus laoreet vel. Sed iaculis odio at massa semper finibus. In accumsan volutpat lectus id faucibus. Nam id consequat arcu. Praesent semper blandit mi, non lobortis velit dignissim quis. In non orci vehicula ligula accumsan vehicula. Morbi vehicula et justo eget tincidunt. Integer vulputate nulla sed suscipit pulvinar. Phasellus nibh turpis, interdum eu purus porta, lacinia ornare eros. Integer consequat metus libero. Proin pharetra eros vel orci dapibus convallis. Mauris condimentum dictum felis, at bibendum lacus dignissim in.
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| - | Cras semper ipsum ac nunc commodo faucibus. Vestibulum quis justo ullamcorper tortor auctor mattis. Nulla ac tellus vitae ipsum eleifend interdum. Aliquam condimentum dui est, a mattis lorem convallis ut. Sed a nisl aliquam, porta neque non, ultricies erat. Nunc ultrices dolor orci, ut pretium velit interdum ac. Integer enim nulla, consectetur a ipsum a, laoreet efficitur eros. In enim nulla, dignissim eu nisl ac, porta sagittis metus. Nam vulputate, risus sit amet consectetur congue, risus odio ultrices neque, a ultrices magna sem ac dui. Nullam sapien velit, suscipit non vulputate id, aliquam sed sapien. Duis et ullamcorper sem.
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| - | Lorem ipsum dolor sit amet, consectetur adipiscing elit. Phasellus commodo ante id mauris scelerisque ullamcorper. Praesent risus enim, tincidunt quis est sed, aliquet sagittis velit. Maecenas purus eros, maximus ac dignissim vel, venenatis iaculis sem. Praesent vel pellentesque eros. Pellentesque eget dictum dolor. Quisque facilisis turpis vitae felis feugiat elementum. Morbi in velit diam. Sed imperdiet dolor ac quam pulvinar, eu pellentesque libero laoreet. Mauris maximus, purus in ultrices dapibus, libero ante vehicula leo, a pharetra lacus leo faucibus purus. Interdum et malesuada fames ac ante ipsum primis in faucibus. Suspendisse potenti. Cras pellentesque nunc est, a aliquet dui fermentum sit amet.
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| - | Nam volutpat vel leo quis rhoncus. Pellentesque orci odio, dignissim eget auctor sodales, lobortis sit amet metus. Integer in tortor et erat vehicula condimentum ac a mi. Nulla ac iaculis nibh. Morbi bibendum pretium quam, at consectetur diam. Pellentesque in turpis nec sem varius sagittis in at eros. Maecenas euismod orci sed ultrices dignissim. Phasellus vitae elit imperdiet, tristique lectus convallis, vulputate sapien. Integer vel arcu lobortis, semper lacus eu, porta eros. Ut elementum pharetra placerat. In in felis gravida, ultrices leo sed, ultricies mauris.
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| - | Fusce purus felis, tincidunt non diam a, hendrerit viverra dolor. Suspendisse condimentum metus molestie dolor sagittis faucibus. Sed ut mauris aliquam orci luctus maximus. Curabitur luctus tempus quam, id ultricies neque tristique vitae. Mauris gravida vulputate nisi vel luctus. Aliquam iaculis aliquet tortor in pulvinar. Integer facilisis sit amet sapien at egestas. In gravida mi lectus, nec facilisis mi dapibus ac. Praesent quis viverra velit, sed ullamcorper neque. Fusce volutpat nulla diam, ut scelerisque purus egestas vitae. Suspendisse aliquet diam et est accumsan, in feugiat nisi sodales. Ut convallis, enim in eleifend porta, urna dui sodales orci, sit amet hendrerit orci nunc ac elit. Duis faucibus sodales purus. Morbi vitae arcu leo. Mauris egestas ultricies augue, at viverra mi vulputate ac. Suspendisse nec tincidunt justo.
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| - | Maecenas rhoncus mauris id ipsum sodales, ut dictum nisl rutrum. Aliquam commodo est dolor, vel cursus risus accumsan in. Duis sed lectus eu quam bibendum scelerisque sit amet iaculis metus. Aliquam et ullamcorper nisl, non vestibulum lacus. Nulla suscipit urna enim, et convallis tellus laoreet vel. Sed iaculis odio at massa semper finibus. In accumsan volutpat lectus id faucibus. Nam id consequat arcu. Praesent semper blandit mi, non lobortis velit dignissim quis. In non orci vehicula ligula accumsan vehicula. Morbi vehicula et justo eget tincidunt. Integer vulputate nulla sed suscipit pulvinar. Phasellus nibh turpis, interdum eu purus porta, lacinia ornare eros. Integer consequat metus libero. Proin pharetra eros vel orci dapibus convallis. Mauris condimentum dictum felis, at bibendum lacus dignissim in.
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| - | Cras semper ipsum ac nunc commodo faucibus. Vestibulum quis justo ullamcorper tortor auctor mattis. Nulla ac tellus vitae ipsum eleifend interdum. Aliquam condimentum dui est, a mattis lorem convallis ut. Sed a nisl aliquam, porta neque non, ultricies erat. Nunc ultrices dolor orci, ut pretium velit interdum ac. Integer enim nulla, consectetur a ipsum a, laoreet efficitur eros. In enim nulla, dignissim eu nisl ac, porta sagittis metus. Nam vulputate, risus sit amet consectetur congue, risus odio ultrices neque, a ultrices magna sem ac dui. Nullam sapien velit, suscipit non vulputate id, aliquam sed sapien. Duis et ullamcorper sem.
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