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Team:Tec-Monterrey
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| + | -ms-filter: "FlipH";" src="https://static.igem.org/mediawiki/2014/3/37/ITESM14_Home.png"></span> | ||
| + | <span><h1 class="col-md-10 text-center"style="color: #f4ff3b; font-size: 5vw; padding: 0; margin: 0 0 30px 10%; font-weight: lighter;"><i>"Using a Trojan horse strategy to kill cancerous cells"</i></h1></span> | ||
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| + | <hr> | ||
| + | <h1>Project Description</h1> | ||
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| + | By harnessing the inherent ability of facultative anaerobic bacteria to colonize and grow in tumoral environments, this project aims to develop a bacterial cancer therapy. A genetically modified <em>E. coli</em> strain will have knockouts in the <em>lpp</em> and <em>msbB</em> genes, which encode for the Braun's lipoprotein and for the myristic acid moiety transporter of the lipopolysaccharide, respectively. These genes are known to trigger an immune response in the human body; by deleting them, the impact originating from a bacterial intravenous administration will be reduced. Furthermore, these bacteria will produce M13-modified bacteriophages under the control of a quorum sensing system. The resulting phages will be capable of binding to the GRP78 receptor, which is usually overexpressed in cancerous cells. Subsequently, they will internalize and transfect the cancerous cells with two different genes: apoptin, responsible for an apoptotic protein specific for cancerous cells which will be regulated by a constitutive promoter and a survivin siRNA. The latter will inhibit the uncontrollable growth characteristic of cancer cells, making them more vulnerable to apoptosis. </b> | ||
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| + | <a href="https://2014.igem.org/Team:Tec-Monterrey/ITESM14_project.html#tab_module1"> | ||
| + | <img class="img img-responsive" src="https://static.igem.org/mediawiki/2014/2/21/ITESM14_module1-banner.png"> | ||
| + | <h1 class="col-md-10 text-center" class="module-title">Attenuation</h1> | ||
| + | <span class="texto-modulos col-md-10 text-center"> | ||
| + | <p class="module-description">The module talks about the a knockout of two genes of the E. coli endotoxins to attenuate the immunological response of the patients.</p> | ||
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| + | <a href="https://2014.igem.org/Team:Tec-Monterrey/ITESM14_project.html#tab_module2"> | ||
| + | <img class="img img-responsive" src="https://static.igem.org/mediawiki/2014/1/11/ITESM14_module2-banner.png"> | ||
| + | <h1 class="col-md-10 col-sm-10 col-xs-10 text-center" class="module-title">Phage engineering</h1> | ||
| + | <span class="texto-modulos col-md-10 text-center"> | ||
| + | <p class="module-description">Modifications were made in the phage M13, so it could infect cancerous cells and be produced using Quorum sensing.</p> | ||
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| + | <div class="col-md-3 col-sm-3 col-xs-6"> | ||
| + | <a href="https://2014.igem.org/Team:Tec-Monterrey/ITESM14_project.html#tab_module3"> | ||
| + | <img class="img img-responsive" src="https://static.igem.org/mediawiki/2014/d/d0/ITESM14_module3-banner.png"> | ||
| + | <h1 class="col-md-10 col-sm-10 col-xs-10 text-center" class="module-title">Quorum sensing</h1> | ||
| + | <span class="texto-modulos col-md-10 text-center"> | ||
| + | <p class="module-description">This system was implemented in the knock-out and is used to cause a coordinated attack from the bacteria that infects the phage.</p> | ||
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| + | </a> | ||
| + | </div> | ||
| + | <div class="col-md-3 col-sm-3 col-xs-6"> | ||
| + | <a href="https://2014.igem.org/Team:Tec-Monterrey/ITESM14_project.html#tab_module4"> | ||
| + | <img class="img img-responsive" src="https://static.igem.org/mediawiki/2014/c/c1/ITESM14_module4-banner.png"> | ||
| + | <h1 class="col-md-10 col-sm-10 col-xs-10 text-center" class="module-title">Effectors</h1> | ||
| + | <span class="texto-modulos col-md-10 text-center"> | ||
| + | <p class="module-description">The action of our effectors, apoptin and siRNA, against cancerous cells is explained here.</p> | ||
| + | </span> | ||
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Latest revision as of 03:45, 18 October 2014
"Using a Trojan horse strategy to kill cancerous cells"
Project Description
By harnessing the inherent ability of facultative anaerobic bacteria to colonize and grow in tumoral environments, this project aims to develop a bacterial cancer therapy. A genetically modified E. coli strain will have knockouts in the lpp and msbB genes, which encode for the Braun's lipoprotein and for the myristic acid moiety transporter of the lipopolysaccharide, respectively. These genes are known to trigger an immune response in the human body; by deleting them, the impact originating from a bacterial intravenous administration will be reduced. Furthermore, these bacteria will produce M13-modified bacteriophages under the control of a quorum sensing system. The resulting phages will be capable of binding to the GRP78 receptor, which is usually overexpressed in cancerous cells. Subsequently, they will internalize and transfect the cancerous cells with two different genes: apoptin, responsible for an apoptotic protein specific for cancerous cells which will be regulated by a constitutive promoter and a survivin siRNA. The latter will inhibit the uncontrollable growth characteristic of cancer cells, making them more vulnerable to apoptosis.
