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Team:Evry/Notebook/CellCharacterization/Transformation/08-23-2014
From 2014.igem.org
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| - | <u> <b> Amplification of plasmids pBBR1MCS and pRhokHI-2 in E.Coli Bl21</b> </u> <br> | + | <u> <b> <big><FONT COLOR=#003333>Amplification of plasmids pBBR1MCS and pRhokHI-2 in E.Coli Bl21</font></big></b> </u> <br> |
Two plasmids pBBR1MCS and pRhokHI-2 were generously sent by Dr Thomas DREPPER (University Duesseldorf, Dept. of Biology, Institute of Molecular Enzyme Technology) | Two plasmids pBBR1MCS and pRhokHI-2 were generously sent by Dr Thomas DREPPER (University Duesseldorf, Dept. of Biology, Institute of Molecular Enzyme Technology) | ||
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<img src="https://static.igem.org/mediawiki/2014/f/f2/Plamids.jpg" alt="plasmidS"/> | <img src="https://static.igem.org/mediawiki/2014/f/f2/Plamids.jpg" alt="plasmidS"/> | ||
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| - | Bowth were transfered into Bl21 electro-competent cells by electroporation (two replicate) | + | Bowth were transfered into Bl21 electro-competent cells by electroporation (two replicate)(1µL of plasmid for 40µL of cells, 1800V). <br> After a liquid culture of three hours, cells were plated on LB 1X + Cam (1:1000) and let in incubator overnight.</p> |
Latest revision as of 22:21, 17 October 2014
Amplification of plasmids pBBR1MCS and pRhokHI-2 in E.Coli Bl21
Two plasmids pBBR1MCS and pRhokHI-2 were generously sent by Dr Thomas DREPPER (University Duesseldorf, Dept. of Biology, Institute of Molecular Enzyme Technology)
Bowth were transfered into Bl21 electro-competent cells by electroporation (two replicate)(1µL of plasmid for 40µL of cells, 1800V).
After a liquid culture of three hours, cells were plated on LB 1X + Cam (1:1000) and let in incubator overnight.
