"
Team:Sumbawagen
From 2014.igem.org
(Difference between revisions)
| (69 intermediate revisions not shown) | |||
| Line 1: | Line 1: | ||
| - | <html | + | <html xlns="http://www.w3.org/1999/xhtml" xml:lang="en" lang="en" dir="ltr"> |
<head> | <head> | ||
<meta http-equiv="Content-Type" content="text/html; charset=utf-8" /> | <meta http-equiv="Content-Type" content="text/html; charset=utf-8" /> | ||
| Line 10: | Line 10: | ||
<style type="text/css" media="screen, projection">/*<![CDATA[*/ | <style type="text/css" media="screen, projection">/*<![CDATA[*/ | ||
@import "/wiki/skins/common/shared.css?270"; | @import "/wiki/skins/common/shared.css?270"; | ||
| - | @import "/wiki/skins/igem/main. | + | @import "/wiki/skins/igem/main.cs70"; |
/*]]>*/</style> | /*]]>*/</style> | ||
<link rel="stylesheet" type="text/css" media="print" href="/wiki/skins/common/commonPrint.css?270" /> | <link rel="stylesheet" type="text/css" media="print" href="/wiki/skins/common/commonPrint.css?270" /> | ||
| Line 126: | Line 126: | ||
swfobject.registerObject("myFlashContent", "9.0.0", "expressInstall.swf"); | swfobject.registerObject("myFlashContent", "9.0.0", "expressInstall.swf"); | ||
</script> | </script> | ||
| - | <title>iGEM | + | <title>iGEM Sumbawagen 2014 · Econey</title> |
<meta name="viewport" content="width=device-width, initial-scale=1.0"> | <meta name="viewport" content="width=device-width, initial-scale=1.0"> | ||
| - | <meta name="description" content="A synthetic biology project called | + | <meta name="description" content="A synthetic biology project called Econey from the Sumbawagen iGEM Team."> |
<meta name="author" content="Sumbawagen Team 2014"> | <meta name="author" content="Sumbawagen Team 2014"> | ||
| Line 176: | Line 176: | ||
<ul class="nav"> | <ul class="nav"> | ||
<li class="active"><a href="https://2014.igem.org/Team:Sumbawagen">Home</a></li> | <li class="active"><a href="https://2014.igem.org/Team:Sumbawagen">Home</a></li> | ||
| - | + | ||
| - | + | ||
<li class="dropdown"> | <li class="dropdown"> | ||
<a href="#" class="dropdown-toggle" data-toggle="dropdown">Overviews <b class="caret"></b></a> | <a href="#" class="dropdown-toggle" data-toggle="dropdown">Overviews <b class="caret"></b></a> | ||
<ul class="dropdown-menu"> | <ul class="dropdown-menu"> | ||
| - | + | <li><a href="https://2014.igem.org/Team:Sumbawagen/overviews/Econey_Project"> Econey Project </a></li> | |
| - | + | </ul> | |
| - | <li><a href="https://2014.igem.org/Team:Sumbawagen/ | + | |
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
</li> | </li> | ||
| Line 196: | Line 190: | ||
<a href="https://2014.igem.org/Team:Sumbawagen/Team" class="dropdown-toggle" data-toggle="dropdown">Project <b class="caret"></b></a> | <a href="https://2014.igem.org/Team:Sumbawagen/Team" class="dropdown-toggle" data-toggle="dropdown">Project <b class="caret"></b></a> | ||
<ul class="dropdown-menu"> | <ul class="dropdown-menu"> | ||
| - | + | ||
| + | <li><a href="https://2014.igem.org/Team:Sumbawagen/project/econey">Econey</a></li> | ||
| + | <li><a href="https://2014.igem.org/Team:Sumbawagen/project/result">Result</a></li> | ||
| + | <li><a href="https://2014.igem.org/Team:Sumbawagen/project/software">Software</a></li> | ||
| + | <li><a href="https://2014.igem.org/Team:Sumbawagen/parts">Parts</a></li> | ||
| + | <li><a href="https://2014.igem.org/Team:Sumbawagen/Safety">Safety</a></li> | ||
| + | <li><a href="https://2014.igem.org/Team:Sumbawagen/Attributions">Attribution</a></li> | ||
| + | <li><a href="https://2014.igem.org/Team:Sumbawagen/future_direction">Future Direction</a></li> | ||
| + | |||
| + | |||
| + | |||
| + | </ul> | ||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
</li> | </li> | ||
<li class="dropdown"> | <li class="dropdown"> | ||
| - | <a href="#" class="dropdown-toggle" data-toggle="dropdown"> | + | <a href="#" class="dropdown-toggle" data-toggle="dropdown">Notebook <b class="caret"></b></a> |
<ul class="dropdown-menu"> | <ul class="dropdown-menu"> | ||
| - | <li><a href=" | + | |
| + | <li class="nav-header">Daily Notes</li> | ||
| + | <li><a href="https://2014.igem.org/Team:Sumbawagen/Notebook">Policy & Practices</a></li> | ||
| + | <li><a href="https://2014.igem.org/Team:Sumbawagen/Notebook2">Lab</a></li> | ||
| + | <li><a href="https://2014.igem.org/Team:Sumbawagen/Notebook/Protocol">Protocol</a></li> | ||
<!--<li><a href="#">Improvements</a></li>--> | <!--<li><a href="#">Improvements</a></li>--> | ||
</ul> | </ul> | ||
</li> | </li> | ||
| - | + | ||
<li class="dropdown"> | <li class="dropdown"> | ||
| Line 235: | Line 223: | ||
<ul class="dropdown-menu"> | <ul class="dropdown-menu"> | ||
<li><a href="https://2014.igem.org/Team:Sumbawagen/Team">Meet the Team</a></li> | <li><a href="https://2014.igem.org/Team:Sumbawagen/Team">Meet the Team</a></li> | ||
| - | |||
<li><a href="https://2014.igem.org/Team:Sumbawagen/Team/Gallery">Gallery</a></li> | <li><a href="https://2014.igem.org/Team:Sumbawagen/Team/Gallery">Gallery</a></li> | ||
| - | |||
<li><a href="https://2014.igem.org/Team:Sumbawagen/Team/Contact">Contact</a></li> | <li><a href="https://2014.igem.org/Team:Sumbawagen/Team/Contact">Contact</a></li> | ||
<li class="divider"></li> | <li class="divider"></li> | ||
<li class="nav-header">Social Media</li> | <li class="nav-header">Social Media</li> | ||
| - | <li><a href="https://www.facebook.com/ | + | <li><a href="https://www.facebook.com/sumbawagen2014?fref=ts">Facebook</a></li> |
| - | <li><a href=" | + | <li><a href="http://sumbawagen.blogspot.com/">Blog</a></li> |
| - | + | ||
| - | + | ||
</ul> | </ul> | ||
</li> | </li> | ||
<li class="dropdown"> | <li class="dropdown"> | ||
| - | <a href="#" class="dropdown-toggle" data-toggle="dropdown"> | + | <a href="#" class="dropdown-toggle" data-toggle="dropdown">Policy & Practices<!--<sup>*new</sup>--><b class="caret"></b></a> |
<ul class="dropdown-menu"> | <ul class="dropdown-menu"> | ||
| - | <li><a href=" | + | |
| - | + | <li><a href="https://2014.igem.org/Team:Sumbawagen/Human_practice/Policy"> | |
| + | <strong>Policy & Practices</strong> | ||
<div style=" | <div style=" | ||
font-size: 100%; | font-size: 100%; | ||
| Line 266: | Line 251: | ||
</a></li> | </a></li> | ||
| - | + | <li class="divider"></li> | |
| - | + | ||
| - | + | ||
| - | < | + | |
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
</a></li> | </a></li> | ||
| + | <li><a href="https://2014.igem.org/Team:Sumbawagen/Human_practice/survey">Survey</a></li> | ||
| + | <li><a href="https://2014.igem.org/Team:Sumbawagen/Human_practice/Biotech">Biotech Camp</a></li> | ||
| + | <li><a href="https://2014.igem.org/Team:Sumbawagen/Human_practice/meet_the">Meet the farmers</a></li> | ||
| + | <li><a href="https://2014.igem.org/Team:Sumbawagen/Human_practice/meet">Meet US Ambassador</a></li> | ||
| + | <li><a href="https://2014.igem.org/Team:Sumbawagen/Human_practice/econey">@america</a></li> | ||
| + | <li><a href="https://2014.igem.org/Team:Sumbawagen/Human_practice/moyo">Moyo Festival</a></li> | ||
| + | <li><a href="https://2014.igem.org/Team:Sumbawagen/Human_practice/virtual">Virtual Outreach</a></li> | ||
| + | <li><a href="https://2014.igem.org/Team:Sumbawagen/Human_practice/Support">Responses and supports</a></li> | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
</ul> | </ul> | ||
</li> | </li> | ||
| Line 305: | Line 270: | ||
<li class="dropdown"> | <li class="dropdown"> | ||
| - | <a href="#" class="dropdown-toggle" data-toggle="dropdown"> | + | <a href="#" class="dropdown-toggle" data-toggle="dropdown">Interlab Study <b class="caret"></b></a> |
<ul class="dropdown-menu"> | <ul class="dropdown-menu"> | ||
| - | <li><a href=" | + | <li><a href="https://2014.igem.org/Team:Sumbawagen/interlabstudy/results">Results</a></li> |
| - | + | </ul> | |
| - | + | ||
</li> | </li> | ||
| Line 335: | Line 299: | ||
<div class="carousel-inner"> | <div class="carousel-inner"> | ||
<div class="item active"><!-- add the active class to any slider you want shown first --> | <div class="item active"><!-- add the active class to any slider you want shown first --> | ||
| - | <img src="https://static.igem.org/mediawiki/2014/ | + | <img src="https://static.igem.org/mediawiki/2014/7/7e/Sumbawa_baner.png" style="width:100%;height:100%"> |
</div> | </div> | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
<div class="item"><!-- add the active class to any slider you want shown first --> | <div class="item"><!-- add the active class to any slider you want shown first --> | ||
<img src="https://static.igem.org/mediawiki/2014/a/af/Banner_SBG3.jpg" style="width:100%;height:100%"> | <img src="https://static.igem.org/mediawiki/2014/a/af/Banner_SBG3.jpg" style="width:100%;height:100%"> | ||
| Line 362: | Line 321: | ||
<p> | <p> | ||
| - | BBa_J04450 has a lac promoter and mRFP gene, thus E. coli harboring this plasmid will give red color when the bacteria were grown with lactose or IPTG. Oppositely, the expression of mRFP will be decreased when glucose was present in medium through a mechanism called catabolite repression. We use this phenomenon to measure the concentration of glucose in honey by calibrating the color of E. coli medium using android-based mobile phone. Creation of novel circuit which consists of constitutive promoter followed by either Adenylate Cyclase or IIAGlc genes, which placed downward lac-mRFP circuit, may affect the sensitivity of catabolite repression. We expect an engineered E. coli, which shows red color expression in different range of glucose concentration. By changing mRFP gene with amilCP, a blue fluorescent protein, we may obtain E. coli expressing either red or blue color when different honey added to the medium due to different glucose concentration.<br><br> | + | BBa_J04450 has a lac promoter and mRFP gene, thus E. coli harboring this plasmid will give red color when the bacteria were grown with lactose or IPTG. Oppositely, the expression of mRFP will be decreased when glucose was present in medium through a mechanism called catabolite repression. We use this phenomenon to measure the concentration of glucose in honey by calibrating the color of E. coli medium using android-based mobile phone. Creation of novel circuit which consists of constitutive promoter followed by either Adenylate Cyclase or IIAGlc genes, which placed downward lac-mRFP circuit, may affect the sensitivity of catabolite repression. We expect an engineered E. coli, which shows red color expression in different range of glucose concentration. By changing mRFP gene with amilCP, a blue fluorescent protein, we may obtain E. coli expressing either red or blue color when different honey added to the medium due to different glucose concentration.<br><br></p> |
| - | |||
| - | |||
<br> | <br> | ||
</div> | </div> | ||
| - | |||
| - | |||
| - | |||
| - | + | <div class="span6" style="padding-top:40px" > | |
| + | <iframe width="460" height="315" src="//www.youtube.com/embed/XICTHe_YXS8" frameborder="0" allowfullscreen></iframe> | ||
| + | </div> | ||
</div><!-- Row End --> | </div><!-- Row End --> | ||
| Line 395: | Line 351: | ||
<img id="image-6" src="https://static.igem.org/mediawiki/2014/b/b8/Home_SBG.jpg" style="width:220px;height:220px;"/> | <img id="image-6" src="https://static.igem.org/mediawiki/2014/b/b8/Home_SBG.jpg" style="width:220px;height:220px;"/> | ||
<span class="caption scale-caption" style="text-align:justify"> | <span class="caption scale-caption" style="text-align:justify"> | ||
| - | <p><b style="font-size:16px">Home</b><br><br> | + | <p><b style="font-size:16px">Home</b><br><br> </p> |
</span> | </span> | ||
</div> | </div> | ||
| Line 406: | Line 362: | ||
<!-- Image Caption 6 --> | <!-- Image Caption 6 --> | ||
<div id="box-6" class="box"> | <div id="box-6" class="box"> | ||
| - | <img id="image-6" src="https://static.igem.org/mediawiki/2014/ | + | <img id="image-6" src="https://static.igem.org/mediawiki/2014/b/b0/Humanpractice_SBG.png" style="width:220px;height:220px;"/> |
<span class="caption scale-caption" style="text-align:justify"> | <span class="caption scale-caption" style="text-align:justify"> | ||
| - | <p><b style="font-size:16px;"> | + | <p><b style="font-size:16px;">Policy & Practices </b><br><br> </p> |
</span> | </span> | ||
</div> | </div> | ||
| Line 421: | Line 377: | ||
<img id="image-6" src="https://static.igem.org/mediawiki/2014/f/fa/Team_SBG.jpg" style="width:220px;height:220px;"/> | <img id="image-6" src="https://static.igem.org/mediawiki/2014/f/fa/Team_SBG.jpg" style="width:220px;height:220px;"/> | ||
<span class="caption scale-caption" style="text-align:justify"> | <span class="caption scale-caption" style="text-align:justify"> | ||
| - | <p><b style="font-size:16px;">Team</b><br><br> | + | <p><b style="font-size:16px;">Team</b><br><br> </p> |
</span> | </span> | ||
</div> | </div> | ||
| Line 435: | Line 391: | ||
<span class="caption scale-caption" style="text-align:justify"> | <span class="caption scale-caption" style="text-align:justify"> | ||
| - | <p><b style="font-size:16px;">ECONEY Project </b><br><br> | + | <p><b style="font-size:16px;">ECONEY Project </b><br><br></p> |
| - | + | ||
| + | </span> | ||
</div> | </div> | ||
</div></a> | </div></a> | ||
| Line 442: | Line 399: | ||
</div> | </div> | ||
| + | <!-- Title --> | ||
| + | <div class="page-header"> | ||
| + | <center><h2><b><strong>Acknowledgment<strong></b> </h2></center> | ||
| + | <center><img src="https://static.igem.org/mediawiki/2014/7/75/BLKM.png"style="width:145px;height:225px;"/><br></center> | ||
| + | |||
| + | </div> | ||
| + | <!-- Title End --> | ||
| + | |||
| + | <!-- Title --> | ||
| + | <div class="page-header"> | ||
| + | <center><h2><b><strong>Sponsors</strong></b> </h2></center> | ||
| + | <center><img src="https://static.igem.org/mediawiki/2014/8/8b/All_sponsor.png"style="width:900px;height:260px;"/><br></center> | ||
</div><!-- End Page Content --> | </div><!-- End Page Content --> | ||
| Line 518: | Line 487: | ||
</div> | </div> | ||
<!-- Served in 0.167 secs. --></body> | <!-- Served in 0.167 secs. --></body> | ||
| + | |||
| + | |||
| + | </p> | ||
| + | <!-- | ||
| + | NewPP limit report | ||
| + | Preprocessor node count: 4/1000000 | ||
| + | Post-expand include size: 0/2097152 bytes | ||
| + | Template argument size: 0/2097152 bytes | ||
| + | Expensive parser function count: 0/100 | ||
| + | --> | ||
| + | |||
| + | <!-- Saved in parser cache with key 2014_igem_org:pcache:idhash:1032-0!1!0!!en!2 and timestamp 20141016110802 --> | ||
| + | <div class="printfooter"> | ||
| + | Retrieved from "<a href="https://2014.igem.org/Team:Sumbawagen">https://2014.igem.org/Team:Sumbawagen</a>"</div> | ||
| + | <div id="catlinks"><div id='catlinks' class='catlinks catlinks-allhidden'></div></div> <!-- end content --> | ||
| + | <div class="visualClear"></div> | ||
| + | </div> | ||
| + | </div> | ||
| + | <!-- PAGE FOOTER -- ITEMS FROM COLUMN ! HAVE BEEN MOVED HERE -- RDR --> | ||
| + | <div class="visualClear"></div> | ||
| + | <div id='footer-box' class='noprint'> | ||
| + | <div id="footer"> | ||
| + | <div id="f-poweredbyico"><a href="http://www.mediawiki.org/"><img src="/wiki/skins/common/images/poweredby_mediawiki_88x31.png" height="31" width="88" alt="Powered by MediaWiki" /></a></div> <div id="f-copyrightico"><a href="http://creativecommons.org/licenses/by/3.0/"><img src="http://i.creativecommons.org/l/by/3.0/88x31.png" alt="Attribution 3.0 Unported" width="88" height="31" /></a></div> <ul id="f-list"> | ||
| + | |||
| + | |||
| + | <!-- Recentchanges is not handles well DEBUG --> | ||
| + | <li id="t-recentchanges"><a href="/Special:RecentChanges" | ||
| + | title='Recent changes'>Recent changes</a></li> | ||
| + | |||
| + | <li id="t-whatlinkshere"><a href="/Special:WhatLinksHere/Team:Sumbawagen" | ||
| + | title="List of all wiki pages that link here [j]" accesskey="j">What links here</a></li> | ||
| + | |||
| + | <li id="t-recentchangeslinked"><a href="/Special:RecentChangesLinked/Team:Sumbawagen" | ||
| + | title="Recent changes in pages linked from this page [k]" accesskey="k">Related changes</a></li> | ||
| + | |||
| + | |||
| + | |||
| + | <li id="t-upload"><a href="/Special:Upload" | ||
| + | title="Upload files [u]" accesskey="u">Upload file</a> | ||
| + | </li> | ||
| + | <li id="t-specialpages"><a href="/Special:SpecialPages" | ||
| + | title="List of all special pages [q]" accesskey="q">Special pages</a> | ||
| + | </li> | ||
| + | <li><a href='/Special:Preferences'>My preferences</a></li> | ||
| + | </ul> | ||
| + | </div> <!-- close footer --> | ||
| + | <div id='footer'> | ||
| + | <ul id="f-list"> | ||
| + | |||
| + | <li id="t-print"><a href="/wiki/index.php?title=Team:Sumbawagen&printable=yes" | ||
| + | title="Printable version of this page [p]" accesskey="p">Printable version</a> | ||
| + | </li> | ||
| + | |||
| + | <li id="t-permalink"><a href="/wiki/index.php?title=Team:Sumbawagen&oldid=269751" | ||
| + | title="Permanent link to this revision of the page">Permanent link</a> | ||
| + | </li> | ||
| + | |||
| + | |||
| + | <li id="privacy"><a href="/2014.igem.org:Privacy_policy" title="2014.igem.org:Privacy policy">Privacy policy</a></li> | ||
| + | <li id="disclaimer"><a href="/2014.igem.org:General_disclaimer" title="2014.igem.org:General disclaimer">Disclaimers</a></li> | ||
| + | </ul> | ||
| + | </div> <!-- close footer --> | ||
| + | </div> <!-- close footer-box --> | ||
| + | |||
| + | <script>if (window.runOnloadHook) runOnloadHook();</script> | ||
| + | </div> | ||
| + | <!-- Served in 0.086 secs. --></body> | ||
</html> | </html> | ||
Latest revision as of 07:50, 19 February 2015
Team:Sumbawagen/Team
From 2014.igem.org
Project Description
BBa_J04450 has a lac promoter and mRFP gene, thus E. coli harboring this plasmid will give red color when the bacteria were grown with lactose or IPTG. Oppositely, the expression of mRFP will be decreased when glucose was present in medium through a mechanism called catabolite repression. We use this phenomenon to measure the concentration of glucose in honey by calibrating the color of E. coli medium using android-based mobile phone. Creation of novel circuit which consists of constitutive promoter followed by either Adenylate Cyclase or IIAGlc genes, which placed downward lac-mRFP circuit, may affect the sensitivity of catabolite repression. We expect an engineered E. coli, which shows red color expression in different range of glucose concentration. By changing mRFP gene with amilCP, a blue fluorescent protein, we may obtain E. coli expressing either red or blue color when different honey added to the medium due to different glucose concentration.
Acknowledgment
Sponsors
