"
Team:Evry/Notebook/CellCharacterization/Antibiotic test/08-23-2014
From 2014.igem.org
(Difference between revisions)
| (6 intermediate revisions not shown) | |||
| Line 10: | Line 10: | ||
| - | <u>Antibiotics' tests on MB 1X plates - Results Day3</u> <br> | + | <u><big><b><FONT COLOR=#003333>Antibiotics' tests on MB 1X plates - Results Day3</font></b></big></u> <br> |
Results of antibiotics' tests on plate are calculated as percentage of bacteria's growth by compared with the controle case. | Results of antibiotics' tests on plate are calculated as percentage of bacteria's growth by compared with the controle case. | ||
Pseudovibrio coming from culture with antibiotics (part B on plates), are taken from antibiotics' range in M9 at Day2.<br> | Pseudovibrio coming from culture with antibiotics (part B on plates), are taken from antibiotics' range in M9 at Day2.<br> | ||
| Line 28: | Line 28: | ||
<img src="https://static.igem.org/mediawiki/2014/2/22/Erm_MB_J3_Graphe%282%29.jpg" alt="Erm" /> | <img src="https://static.igem.org/mediawiki/2014/2/22/Erm_MB_J3_Graphe%282%29.jpg" alt="Erm" /> | ||
| - | <img src="https://static.igem.org/mediawiki/2014/c/c2/Graphe_J3-MB1X%282%29.jpg" alt="Graphs" /> | + | <div align="center"><img src="https://static.igem.org/mediawiki/2014/c/c2/Graphe_J3-MB1X%282%29.jpg" alt="Graphs" style="width:80%;height:80%"/></div> |
<u>Antibiotics' tests on MB 0.5X plates - Results Day3</u> <br> | <u>Antibiotics' tests on MB 0.5X plates - Results Day3</u> <br> | ||
| Line 34: | Line 34: | ||
Pseudovibrio coming from culture with antibiotics (part B on plates), are taken from antibiotics' range in M9 at Day2.<br> | Pseudovibrio coming from culture with antibiotics (part B on plates), are taken from antibiotics' range in M9 at Day2.<br> | ||
<li>Chloramphenicol | <li>Chloramphenicol | ||
| - | <img src="" alt="Cam" /> | + | <img src="https://static.igem.org/mediawiki/2014/b/bc/Cam_MB05_J3.jpg" alt="Cam" /> |
<li>Kanamycin | <li>Kanamycin | ||
| - | <img src="" alt="Kan" /> | + | <img src="https://static.igem.org/mediawiki/2014/6/66/Kan_MB05_J3.jpg" alt="Kan" /> |
<li>Ampicilin | <li>Ampicilin | ||
| - | <img src="" alt="Amp" /> | + | <img src="https://static.igem.org/mediawiki/2014/a/aa/Amp_MB05_J3.jpg" alt="Amp" /> |
<li>Tetracyclin | <li>Tetracyclin | ||
| - | <img src="" alt="Tet" /> | + | <img src="https://static.igem.org/mediawiki/2014/6/64/Tetra_MB05_J3.jpg" alt="Tet" /> |
<li>Erythromycin | <li>Erythromycin | ||
| - | <img src="" alt="Erm" /> | + | <img src="https://static.igem.org/mediawiki/2014/2/2c/Erythromycine_MB05_J3.jpg" alt="Erm" /> |
| - | <img src="https://static.igem.org/mediawiki/2014/4/44/Graphe_J3-MB05X.jpg" alt="Graphs" /> | + | <div align="center"><img src="https://static.igem.org/mediawiki/2014/4/44/Graphe_J3-MB05X.jpg" alt="Graphs" style="width:80%;height:80%"/></div> |
<u>Antibiotics' tests on M9 1X plates - Results Day3</u> <br> | <u>Antibiotics' tests on M9 1X plates - Results Day3</u> <br> | ||
| - | Results of antibiotics' tests on plate are calculated as percentage of bacteria's growth by compared with the controle case. | + | <p>Results of antibiotics' tests on plate are calculated as percentage of bacteria's growth by compared with the controle case. |
| - | Pseudovibrio coming from culture with antibiotics (part B on plates), are taken from antibiotics' range in M9 at Day2.<br> | + | Pseudovibrio coming from culture with antibiotics (part B on plates), are taken from antibiotics' range in M9 at Day2.<br></p> |
<li>Chloramphenicol | <li>Chloramphenicol | ||
| - | <img src="" alt="Cam" /> | + | <img src="https://static.igem.org/mediawiki/2014/c/c7/Chloramphenicol_M9s_J3.jpg" alt="Cam" /> |
<li>Kanamycin | <li>Kanamycin | ||
| - | <img src="" alt="Kan" /> | + | <img src="https://static.igem.org/mediawiki/2014/f/fd/Kanamycine_M9s_J3.jpg" alt="Kan" /> |
<li>Ampicilin | <li>Ampicilin | ||
| - | <img src="" alt="Amp" /> | + | <img src="https://static.igem.org/mediawiki/2014/f/fe/Ampiciline_M9s_J3.jpg" alt="Amp" /> |
<li>Tetracyclin | <li>Tetracyclin | ||
| - | <img src="" alt="Tet" /> | + | <img src="https://static.igem.org/mediawiki/2014/a/a3/Tetracycline_M9s_J3.jpg" alt="Tet" /> |
<li>Erythromycin | <li>Erythromycin | ||
| - | <img src="" alt="Erm" /> <br> | + | <img src="https://static.igem.org/mediawiki/2014/3/39/Erythromycine_M9s_J3.jpg" alt="Erm" /> <br> |
| - | + | <div align="center"> <img src="https://static.igem.org/mediawiki/2014/0/03/Graphe_J3-M91X.jpg" alt="Graphs" style="width:80%;height:80%"/> </div><br> | |
<br> | <br> | ||
| Line 75: | Line 75: | ||
<li> Tests of Kanamycin's resistance cassette: | <li> Tests of Kanamycin's resistance cassette: | ||
<br> | <br> | ||
| - | <b><u>Graph of Kanamycine's resistance tests in liquid M9 1X-Day1</u></b> | + | |
| - | <img src="https://static.igem.org/mediawiki/2014/ | + | <div align="center"> |
| + | <b><u>Graph of Kanamycine's resistance tests in liquid M9 1X-Day1</u></b> <br> | ||
| + | <img src="https://static.igem.org/mediawiki/2014/0/02/KanR_M9l_J3.jpg" alt="New Graphs with KanR" /> | ||
| + | </div> | ||
<li> Tests of Ampicilin's resistance cassette: | <li> Tests of Ampicilin's resistance cassette: | ||
| - | <br> | + | <br><p> |
| - | Antibiotics' tests in liquid M9 for E.Coli transformed with a plasmid containing an ampicilin resistance cassette were launch. | + | Antibiotics' tests in liquid M9 for E.Coli transformed with a plasmid PSB1A3 containing an ampicilin resistance cassette were launch. |
In two days, these cultures will be plated on MB 1X, MB 0.5X and M9 1X plates | In two days, these cultures will be plated on MB 1X, MB 0.5X and M9 1X plates | ||
| - | + | </p> | |
Latest revision as of 22:19, 17 October 2014
Antibiotics' tests on MB 1X plates - Results Day3
Results of antibiotics' tests on plate are calculated as percentage of bacteria's growth by compared with the controle case.
Pseudovibrio coming from culture with antibiotics (part B on plates), are taken from antibiotics' range in M9 at Day2.
Results of antibiotics' tests on plate are calculated as percentage of bacteria's growth by compared with the controle case. Pseudovibrio coming from culture with antibiotics (part B on plates), are taken from antibiotics' range in M9 at Day2.
Results of antibiotics' tests on plate are calculated as percentage of bacteria's growth by compared with the controle case.
Pseudovibrio coming from culture with antibiotics (part B on plates), are taken from antibiotics' range in M9 at Day2.
Differed launch of antibiotics' tests
Graph of Kanamycine's resistance tests in liquid M9 1X-Day1
Antibiotics' tests in liquid M9 for E.Coli transformed with a plasmid PSB1A3 containing an ampicilin resistance cassette were launch. In two days, these cultures will be plated on MB 1X, MB 0.5X and M9 1X plates
Aug 23
