Team:Evry/Notebook/CellCharacterization/Antibiotic test/08-23-2014
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- | <u>Antibiotics' tests on MB 1X plates - Results Day3</u> <br> | + | <u><big><b><FONT COLOR=#003333>Antibiotics' tests on MB 1X plates - Results Day3</font></b></big></u> <br> |
Results of antibiotics' tests on plate are calculated as percentage of bacteria's growth by compared with the controle case. | Results of antibiotics' tests on plate are calculated as percentage of bacteria's growth by compared with the controle case. | ||
Pseudovibrio coming from culture with antibiotics (part B on plates), are taken from antibiotics' range in M9 at Day2.<br> | Pseudovibrio coming from culture with antibiotics (part B on plates), are taken from antibiotics' range in M9 at Day2.<br> | ||
<li>Chloramphenicol | <li>Chloramphenicol | ||
- | <img src="" alt="Cam" /> | + | <img src="https://static.igem.org/mediawiki/2014/d/d4/Cam_MB_J3_Graphe%282%29.jpg" alt="Cam" /> |
<li>Kanamycin | <li>Kanamycin | ||
- | <img src="" alt="Kan" /> | + | <img src="https://static.igem.org/mediawiki/2014/9/95/Tet_MB_J3_Graphe%282%29.jpg" alt="Kan" /> |
<li>Ampicilin | <li>Ampicilin | ||
- | <img src="" alt="Amp" /> | + | <img src="https://static.igem.org/mediawiki/2014/3/34/Amp_MB_J3_Graphe%282%29.jpg" alt="Amp" /> |
<li>Tetracyclin | <li>Tetracyclin | ||
- | <img src="" alt="Tet" /> | + | <img src="https://static.igem.org/mediawiki/2014/9/95/Tet_MB_J3_Graphe%282%29.jpg" alt="Tet" /> |
<li>Erythromycin | <li>Erythromycin | ||
- | <img src="" alt="Erm" /> | + | <img src="https://static.igem.org/mediawiki/2014/2/22/Erm_MB_J3_Graphe%282%29.jpg" alt="Erm" /> |
- | <img src="https://static.igem.org/mediawiki/2014/c/c2/Graphe_J3-MB1X%282%29.jpg" alt="Graphs" /> | + | <div align="center"><img src="https://static.igem.org/mediawiki/2014/c/c2/Graphe_J3-MB1X%282%29.jpg" alt="Graphs" style="width:80%;height:80%"/></div> |
- | <u>Antibiotics' tests on MB 0.5X plates - Results | + | <u>Antibiotics' tests on MB 0.5X plates - Results Day3</u> <br> |
Results of antibiotics' tests on plate are calculated as percentage of bacteria's growth by compared with the controle case. | Results of antibiotics' tests on plate are calculated as percentage of bacteria's growth by compared with the controle case. | ||
Pseudovibrio coming from culture with antibiotics (part B on plates), are taken from antibiotics' range in M9 at Day2.<br> | Pseudovibrio coming from culture with antibiotics (part B on plates), are taken from antibiotics' range in M9 at Day2.<br> | ||
<li>Chloramphenicol | <li>Chloramphenicol | ||
- | <img src="" alt="Cam" /> | + | <img src="https://static.igem.org/mediawiki/2014/b/bc/Cam_MB05_J3.jpg" alt="Cam" /> |
<li>Kanamycin | <li>Kanamycin | ||
- | <img src="" alt="Kan" /> | + | <img src="https://static.igem.org/mediawiki/2014/6/66/Kan_MB05_J3.jpg" alt="Kan" /> |
<li>Ampicilin | <li>Ampicilin | ||
- | <img src="" alt="Amp" /> | + | <img src="https://static.igem.org/mediawiki/2014/a/aa/Amp_MB05_J3.jpg" alt="Amp" /> |
<li>Tetracyclin | <li>Tetracyclin | ||
- | <img src="" alt="Tet" /> | + | <img src="https://static.igem.org/mediawiki/2014/6/64/Tetra_MB05_J3.jpg" alt="Tet" /> |
<li>Erythromycin | <li>Erythromycin | ||
- | <img src="" alt="Erm" /> | + | <img src="https://static.igem.org/mediawiki/2014/2/2c/Erythromycine_MB05_J3.jpg" alt="Erm" /> |
- | <img src="https://static.igem.org/mediawiki/2014/4/44/Graphe_J3-MB05X.jpg" alt="Graphs" /> | + | <div align="center"><img src="https://static.igem.org/mediawiki/2014/4/44/Graphe_J3-MB05X.jpg" alt="Graphs" style="width:80%;height:80%"/></div> |
- | <u>Antibiotics' tests on M9 1X plates - Results | + | <u>Antibiotics' tests on M9 1X plates - Results Day3</u> <br> |
- | Results of antibiotics' tests on plate are calculated as percentage of bacteria's growth by compared with the controle case. | + | <p>Results of antibiotics' tests on plate are calculated as percentage of bacteria's growth by compared with the controle case. |
- | Pseudovibrio coming from culture with antibiotics (part B on plates), are taken from antibiotics' range in M9 at Day2.<br> | + | Pseudovibrio coming from culture with antibiotics (part B on plates), are taken from antibiotics' range in M9 at Day2.<br></p> |
<li>Chloramphenicol | <li>Chloramphenicol | ||
- | <img src="" alt="Cam" /> | + | <img src="https://static.igem.org/mediawiki/2014/c/c7/Chloramphenicol_M9s_J3.jpg" alt="Cam" /> |
<li>Kanamycin | <li>Kanamycin | ||
- | <img src="" alt="Kan" /> | + | <img src="https://static.igem.org/mediawiki/2014/f/fd/Kanamycine_M9s_J3.jpg" alt="Kan" /> |
<li>Ampicilin | <li>Ampicilin | ||
- | <img src="" alt="Amp" /> | + | <img src="https://static.igem.org/mediawiki/2014/f/fe/Ampiciline_M9s_J3.jpg" alt="Amp" /> |
<li>Tetracyclin | <li>Tetracyclin | ||
- | <img src="" alt="Tet" /> | + | <img src="https://static.igem.org/mediawiki/2014/a/a3/Tetracycline_M9s_J3.jpg" alt="Tet" /> |
<li>Erythromycin | <li>Erythromycin | ||
- | <img src="" alt="Erm" /> | + | <img src="https://static.igem.org/mediawiki/2014/3/39/Erythromycine_M9s_J3.jpg" alt="Erm" /> <br> |
- | + | <div align="center"> <img src="https://static.igem.org/mediawiki/2014/0/03/Graphe_J3-M91X.jpg" alt="Graphs" style="width:80%;height:80%"/> </div><br> | |
+ | |||
+ | <br> | ||
<u>Differed launch of antibiotics' tests</u> <br> | <u>Differed launch of antibiotics' tests</u> <br> | ||
- | <li> Tests of Kanamycin's resistance cassette: <br> | + | <li> Tests of Kanamycin's resistance cassette: |
+ | <br> | ||
- | <img src="https://static.igem.org/mediawiki/2014/ | + | <div align="center"> |
+ | <b><u>Graph of Kanamycine's resistance tests in liquid M9 1X-Day1</u></b> <br> | ||
+ | <img src="https://static.igem.org/mediawiki/2014/0/02/KanR_M9l_J3.jpg" alt="New Graphs with KanR" /> | ||
+ | </div> | ||
- | <li> Tests of Ampicilin's resistance cassette: <br> | + | <li> Tests of Ampicilin's resistance cassette: |
- | Antibiotics' tests in liquid M9 for E.Coli transformed with a plasmid containing an ampicilin resistance cassette were launch. | + | <br><p> |
+ | Antibiotics' tests in liquid M9 for E.Coli transformed with a plasmid PSB1A3 containing an ampicilin resistance cassette were launch. | ||
In two days, these cultures will be plated on MB 1X, MB 0.5X and M9 1X plates | In two days, these cultures will be plated on MB 1X, MB 0.5X and M9 1X plates | ||
- | + | </p> | |
Latest revision as of 22:19, 17 October 2014
![Picture](https://static.igem.org/mediawiki/2013/9/90/EvryBiology.png)
Antibiotics' tests on MB 1X plates - Results Day3
Results of antibiotics' tests on plate are calculated as percentage of bacteria's growth by compared with the controle case.
Pseudovibrio coming from culture with antibiotics (part B on plates), are taken from antibiotics' range in M9 at Day2.
![Cam](https://static.igem.org/mediawiki/2014/d/d4/Cam_MB_J3_Graphe%282%29.jpg)
![Kan](https://static.igem.org/mediawiki/2014/9/95/Tet_MB_J3_Graphe%282%29.jpg)
![Amp](https://static.igem.org/mediawiki/2014/3/34/Amp_MB_J3_Graphe%282%29.jpg)
![Tet](https://static.igem.org/mediawiki/2014/9/95/Tet_MB_J3_Graphe%282%29.jpg)
![Erm](https://static.igem.org/mediawiki/2014/2/22/Erm_MB_J3_Graphe%282%29.jpg)
![Graphs](https://static.igem.org/mediawiki/2014/c/c2/Graphe_J3-MB1X%282%29.jpg)
Results of antibiotics' tests on plate are calculated as percentage of bacteria's growth by compared with the controle case. Pseudovibrio coming from culture with antibiotics (part B on plates), are taken from antibiotics' range in M9 at Day2.
![Cam](https://static.igem.org/mediawiki/2014/b/bc/Cam_MB05_J3.jpg)
![Kan](https://static.igem.org/mediawiki/2014/6/66/Kan_MB05_J3.jpg)
![Amp](https://static.igem.org/mediawiki/2014/a/aa/Amp_MB05_J3.jpg)
![Tet](https://static.igem.org/mediawiki/2014/6/64/Tetra_MB05_J3.jpg)
![Erm](https://static.igem.org/mediawiki/2014/2/2c/Erythromycine_MB05_J3.jpg)
![Graphs](https://static.igem.org/mediawiki/2014/4/44/Graphe_J3-MB05X.jpg)
Results of antibiotics' tests on plate are calculated as percentage of bacteria's growth by compared with the controle case.
Pseudovibrio coming from culture with antibiotics (part B on plates), are taken from antibiotics' range in M9 at Day2.
![Cam](https://static.igem.org/mediawiki/2014/c/c7/Chloramphenicol_M9s_J3.jpg)
![Kan](https://static.igem.org/mediawiki/2014/f/fd/Kanamycine_M9s_J3.jpg)
![Amp](https://static.igem.org/mediawiki/2014/f/fe/Ampiciline_M9s_J3.jpg)
![Tet](https://static.igem.org/mediawiki/2014/a/a3/Tetracycline_M9s_J3.jpg)
![Erm](https://static.igem.org/mediawiki/2014/3/39/Erythromycine_M9s_J3.jpg)
![Graphs](https://static.igem.org/mediawiki/2014/0/03/Graphe_J3-M91X.jpg)
Differed launch of antibiotics' tests
![New Graphs with KanR](https://static.igem.org/mediawiki/2014/0/02/KanR_M9l_J3.jpg)
Antibiotics' tests in liquid M9 for E.Coli transformed with a plasmid PSB1A3 containing an ampicilin resistance cassette were launch. In two days, these cultures will be plated on MB 1X, MB 0.5X and M9 1X plates
Aug 23