Team:UESTC-China

From 2014.igem.org

(Difference between revisions)
 
(331 intermediate revisions not shown)
Line 1: Line 1:
-
<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Strict//EN"
+
<html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en">
-
    "http://www.w3.org/TR/xhtml1/DTD/xhtml1-strict.dtd">
+
<head>
 +
<meta http-equiv="Content-Type" content="text/html;charset=UTF-8" />
 +
<title>Plant Vs HCHO</title>
 +
 
-
<html xmlns="http://www.w3.org/1999/xhtml">
 
-
    <head>
 
-
        <meta name="keywords" content="" />
 
-
        <meta name="description" content="" />
 
-
        <meta http-equiv="content-type" content="text/html; charset=utf-8" />
 
-
<title>IGEM</title>
 
<style>
<style>
-
@font-face {
+
.imageRotation{
-
  font-family: 'PT Sans Narrow';
+
height:500px;
-
  font-style: normal;
+
width:1195px;
-
  font-weight: 400;
+
overflow:hidden;  /*--超出容器的所有元素都不可见--*/
-
  src: local('PT Sans Narrow'), local('PTSans-Narrow'), url(http://themes.googleusercontent.com/static/fonts/ptsansnarrow/v5/UyYrYy3ltEffJV9QueSi4RdbPw3QSf9R-kE0EsQUn2A.woff) format('woff');
+
position:relative;  /*--相对定位--*/
 +
border:10px solid #eee;
 +
bodrer-radius:5px;
 +
-webkit-border-radius:5px;
 +
-moz-border-radius:5px;
 +
}
 +
/*-------------图片容器---------------*/
 +
.imageBox{
 +
position:absolute;  /*--固定定位--*/
 +
height:500px;
 +
top:0px;
 +
left:0px;
 +
overflow:hidden;
 +
}
 +
.imageBox img {
 +
display:block;
 +
height:500px;
 +
width:1195px;
 +
float:left;
 +
border:none;
 +
}
 +
/*-------------标题容器---------------*/
 +
.titleBox{
 +
position:absolute;
 +
bottom:0px;
 +
width:1214px;
 +
height:40px;
 +
overflow:hidden;
 +
}
 +
.titleBox p{
 +
position:absolute;
 +
bottom:-40px;
 +
width:1214px;
 +
height:40px;
 +
margin:0px;
 +
padding:0px 10px;
 +
line-height:40px;
 +
z-index:1;
 +
background-color:#000;
 +
color:#fff;
 +
font-family:"微软雅黑","yahei",calibri, arial, helvetica, sans-serif,Italic;
 +
font-weight: bold;
 +
font-style: sans-serif;
 +
opacity:0.5;
 +
-moz-opacity:0.5;
 +
-webkit-opacity:0.5;
 +
filter:alpha(opacity=50);
 +
}
 +
.titleBox p span{
 +
opacity:1;
 +
-moz-opacity:1;
 +
-webkit-opacity:1;
 +
filter:alpha(opacity=100);
 +
}
 +
.titleBox p.active{
 +
bottom:0px;
 +
}
 +
/*-------------图标容器---------------*/
 +
.icoBox{
 +
position:absolute;  /*--固定定位--*/
 +
bottom:14px;
 +
right:15px;
 +
width:76px;
 +
height:12px;
 +
text-align:center;
 +
line-height:40px;
 +
z-index:2;
 +
}
 +
.icoBox span{
 +
display:block;
 +
float:left;
 +
height:12px;
 +
width:12px;
 +
margin-left:3px;
 +
overflow:hidden;
 +
background:url("https://static.igem.org/mediawiki/2014/a/a2/Ico.png") 0px 0px no-repeat;
 +
cursor:pointer;
 +
}
 +
.icoBox span.active {
 +
background-position:0px -12px;
 +
cursor:default;
 +
}
 +
 
 +
#content {
 +
    position: relative;
 +
    width:  1215px;
 +
#    margin-left: 10px;
 +
margin: 0 auto;
 +
    padding: 5px 5px 5px 5px;;
 +
    background: white;
 +
    color: black;
 +
    border-left: 1px solid #444444;
 +
    border-right: 1px solid #444444;
 +
    line-height: 1.5em;
 +
    z-index: 2;
}
}
-
@font-face {
+
 
-
  font-family: 'Lobster';
+
     
-
  font-style: normal;
+
    html,body,div,span,applet,object,iframe,h1,h2,h3,h4,h5,h6,p,blockquote,pre,a,abbr,acronym,address,big,cite,code,del,dfn,em,font,img,ins,kbd,q,s,samp,small,strike,strong,sub,sup,tt,var,b,u,i,center,dl,dt,dd,ol,ul,li,fieldset,form,label,legend,table,caption,tbody,tfoot,thead,tr,th,td{margin:0;padding:0;border:0;outline:0;font-size:100%;vertical-align:baseline;background:transparent}body{line-height:1}ol,ul{list-style:none}blockquote,q{quotes:none}blockquote:before,blockquote:after,q:before,q:after{content:'';content:none}:focus{outline:0}ins{text-decoration:none}del{text-decoration:line-through}table{border-collapse:collapse;border-spacing:0}
-
  font-weight: 400;
+
 
-
  src: local('Lobster'), url(http://themes.googleusercontent.com/static/fonts/lobster/v8/MWVf-Rwh4GLQVBEwbyI61Q.woff) format('woff');
+
body{
 +
background-color:#bfbfbf;
}
}
-
* {
+
a{
-
margin: 0; padding: 0;
+
text-decoration: none;
 +
color: white;
}
}
-
 
+
a:hover{
-
a {
+
text-decoration: none;
-
color: #8f5038;
+
color: white;
-
text-decoration: underline;
+
}
}
-
 
+
a:active{
-
a:hover {
+
text-decoration: none;
text-decoration: none;
 +
color: white;
}
}
-
 
+
a:visited{
-
body {
+
text-decoration: none;
-
font-size: 10.5pt;
+
color: white;
-
line-height: 1.75em;
+
-
font-family: Arial, sans-serif;
+
-
background: #e1e4e5 url('images/bg.png');
+
-
color: #383939;
+
}
}
-
 
+
#top-section{
-
br.clear {
+
height: 0px;
-
clear: both;
+
border:none;
}
}
-
 
+
#search-controls{
-
h1,h2,h3,h4 {
+
display: none;
-
text-transform: lowercase;
+
-
font-weight: normal;
+
}
}
-
 
+
#p-logo{
-
h2,h3,h4 {
+
display: none;
-
margin-bottom: 1em;
+
-
font-family: "PT Sans Narrow", sans-serif;
+
-
color: #000;
+
}
}
-
 
+
#total-wrap{
-
h2 {
+
position:absolute;
-
font-size: 1.8em;
+
width: 1215px;
 +
left: 50%;
 +
right: 50%;
 +
margin-left: -607px;
 +
color: white;
}
}
 +
#header{
 +
height: 280px;
 +
background-image:url("https://static.igem.org/mediawiki/2014/4/49/New_New_banner.jpg");/* yan change to plant-vs HCHO*/
 +
background-position: top center;
 +
background-repeat: no-repeat;
 +
border-radius:3px;
 +
overflow: hidden;
 +
width: 1214px;
 +
}
 +
#emp{
 +
height:200px;
 +
}
 +
#top-menu{
 +
display: inline-block;
 +
padding-left:20px;
 +
background-color: #394264;
 +
width: 1195px;
-
h3 {
 
-
font-size: 1.4em;
 
}
}
-
img.left {
+
@font-face {
 +
font-family: ubuntu;
 +
src:url(Ubuntu-R.ttf);
 +
}
 +
.top-menu-each{
 +
height: 63px;
 +
 +
font-family: helveticallt,"微软雅黑";
 +
color: white;
 +
font-size: 21px;
float: left;
float: left;
-
margin: 8px 20px 20px 0;
+
cursor: pointer;
 +
font-weight: bold;
}
}
-
img.top {
+
.top-menu-each p{
-
margin: 8px 0 20px 0;
+
float: left;
 +
padding-right: 40px;
 +
padding-left: 40px;
 +
line-height: 63px;
}
}
 +
.arrow{
 +
float: left;
 +
background-image: url("http://fudanigem.phx-2.ifreeurl.com/img/arrow.png");/*needn't change*/
 +
line-height: 63px;
 +
width: 12px;
 +
height: 9px;
 +
margin-left: 9px;
 +
margin-right: 9px;
 +
margin-top: 30px;
 +
position: absolute;
-
p {
 
-
margin-bottom: 1.75em;
 
}
}
-
 
+
.top-menu-each:hover{
-
ul {
+
background-color: #50597B;
-
margin-bottom: 1.75em;
+
border-bottom-style:solid;
 +
border-bottom-width: 2px;
 +
border-bottom-color:#11a8ab;
 +
height: 61px;
 +
}
}
-
 
+
.top-menu-each.click{
-
.imageList {
+
background-color: #50597B;
-
list-style: none;
+
border-bottom-style:solid;
 +
border-bottom-width: 2px;
 +
border-bottom-color:#11a8ab;
 +
height: 61px;
}
}
-
 
+
.top-menu-sub{
-
.imageList li {
+
position: absolute;
-
clear: both;
+
margin-top: 63px;
 +
z-index: 10;
 +
min-width: 215px;
}
}
-
 
+
.top-menu-sub li{
-
#bg {
+
background-color: #394264;
-
background: url('images/highlight.png') top left repeat-x;
+
border-top-style: solid;
 +
border-bottom-style: solid;
 +
border-top-width: 1px;
 +
border-bottom-width: 1px;
 +
border-top-color: #11a8ab;
 +
border-bottom-color: #11a8ab;
 +
padding-left: 30px;
 +
padding-right: 20px;
 +
height: 55px;
 +
line-height: 55px;
 +
cursor:pointer;
}
}
-
 
+
.top-menu-sub li:hover{
-
#banner {
+
background-color: #50597B;
-
top:135px;
+
-
position: relative;
+
-
padding: 28px;
+
-
height: 212px;
+
-
width: 1124px;
+
-
margin: 28px 0 0 0;
+
-
background: #ffffff;
+
-
border-bottom: solid 1px #c0c0c0;
+
}
}
-
 
+
#big-photo{
-
#box1 {
+
margin-top: 5px;
-
width: 500px;
+
height: 500px;
 +
width: 1214px;
overflow: hidden;
overflow: hidden;
-
margin: 0px 0 24px 0;
 
}
}
 +
#big-photo img{
 +
height: 500px;
 +
width: 1214px;
 +
position: absolute;
-
#box2 {
+
}
-
width: 236px;
+
#big-photo-hover{
 +
height: 130px;
 +
padding-left: 35px;
 +
padding-right: 10px;
 +
background-image: url('http://fudanigem.phx-2.ifreeurl.com/img/big-photo-hover.png');/*no change*/
 +
background-repeat: no-repeat;
 +
margin-top: -130px;
 +
position: absolute;
 +
margin-top: 370px;
 +
width: 1169px;
 +
}
 +
@font-face {
 +
font-family: helveticamd;
 +
src:url(HelveticaNeueLTStd-Md.otf);
 +
}
 +
@font-face {
 +
font-family: helveticalt;
 +
src:url(HelveticaNeueLTStd-Lt.otf);
 +
}
 +
#big-photo-hover .header{
 +
font-size: 30px;
 +
line-height: 30px;
 +
font-family: helveticalmd,"微软雅黑";
 +
font-weight: bold;
 +
padding-top: 25px;
 +
margin-bottom: 10px;
 +
}
 +
#big-photo-hover .content{
 +
font-size: 23px;
 +
line-height: 23px;
 +
font-family: helveticallt,"微软雅黑";
 +
}
 +
#big-photo-wrap{
 +
position: absolute;
 +
width: 1214px;
 +
height: 500px;
overflow: hidden;
overflow: hidden;
 +
}
 +
#small-photo{
 +
margin-top: 15px;
 +
display: inline-block;
 +
margin-bottom: 335px;
 +
}
 +
.small-photo-each{
 +
width: 351px;
 +
height: 410px;
 +
padding-top: 10px;
 +
padding-bottom:5px;
 +
padding-left: 10px;
 +
padding-right: 10px;
 +
margin-right: 51px;
float: left;
float: left;
 +
background-color: white;
}
}
-
 
+
.small-photo-each img{
-
#box3 {
+
width: 351px;
-
width: 236px;
+
height: 410px;
-
overflow: hidden;
+
-
margin: 0px 0 0 260px;
+
}
}
-
 
+
.small-photo-each.last{
-
#content {
+
margin-right: 0px;
-
width: 500px;
+
-
margin: 0px 28px 0 312px;
+
-
padding: 28px;
+
-
background: #ffffff;
+
-
border-bottom: solid 1px #c0c0c0;
+
}
}
-
 
+
#footer{
-
#copyright {
+
-
top:100px;
+
height: 180px;
-
position: relative;
+
background-color:white;
-
margin: 28px 0 64px 0;
+
border-top-left-radius:8px;
-
text-align: center;
+
border-top-right-radius:8px;
-
text-shadow: 0 1px 0 #ffffff;
+
margin-top: 20px;
-
color: #787979;
+
}
}
-
 
+
#main-content{
-
#copyright a {
+
position:absolute;
-
color: #787979;
+
margin-top: 40px;
 +
display: inline-block;
}
}
-
 
+
#left-bar{
-
#footer {
+
width: 225px;
-
top:100px;
+
border-radius: 3px;
-
position: relative;
+
overflow: hidden;
-
padding: 28px;
+
position: absolute;
-
margin: 28px 0 0 0;
+
-
width: 1124px;
+
-
background: #636363;
+
-
color: #d3d3d3;
+
-
border-bottom: solid 1px #444444;
+
-
text-shadow: 0 1px 0 #404040;
+
}
}
-
 
+
.left-bar-each{
-
#footer a {
+
height: 60px;
-
color: #eee;
+
background-color: #394264;
 +
font-family: ubuntu,"宋体";
 +
font-size: 18px;
 +
line-height: 60px;
 +
padding-left: 30px;
 +
cursor: pointer;
 +
border-bottom-style: solid;
 +
border-bottom-color: #1f253d;
 +
border-bottom-width: 1px;
}
}
-
 
+
.left-bar-each:hover{
-
#footer h2, #footer h3, #footer h4 {
+
background-color: #50597b;
-
color: #ffffff;
+
}
}
-
 
+
.left-bar-each.top{
-
#footer ul {
+
background-color: #38b7c4;
-
list-style: none;
+
text-align:center;
-
margin-bottom: 0;
+
padding-left: 0px;
}
}
-
 
+
.left-bar-each.top:hover{
-
#footer p {
+
background-color: #38b7c4;
-
margin-bottom: 0;
+
}
}
-
 
+
.left-bar-sub .left-bar-each{
-
#footer ul li {
+
padding-left: 90px;
-
padding: 8px 0 8px 0;
+
-
border-top: dotted 1px #8c8c8c;
+
}
}
-
 
+
#middle-photo{
-
#footer ul li.first {
+
float: left;
-
padding-top: 0;
+
margin-bottom: 445px;
-
border-top: 0;
+
}
}
 +
.middle-photo-each{
 +
padding:9px 9px 9px 9px;
 +
background-color: white;
 +
margin-bottom: 15px;
 +
margin-left: 250px;
-
#footerContent {
+
}
-
width: 812px;
+
.middle-photo-each .middle-content{
 +
height: 400px;
 +
width: 947px;
 +
}
 +
.middle-content img{
float: left;
float: left;
}
}
-
 
+
#examplepic{
-
#footerSidebar {
+
width: 100px;
-
width: 232px;
+
height:60px;
-
margin: 0px 0 0 892px;
+
}
}
-
 
+
.middle-content p{
-
#header {
+
color: black;
-
top:0px;
+
font-size: 16px;
-
overflow:hidden;
+
font-family: "微软雅黑";
-
border-bottom:1px solid #d3d7d8;
+
line-height: 20px;
-
position: fixed;
+
-
padding: 28px;
+
-
height: 126px;
+
-
width: 1124px;
+
-
z-index:1000;
+
-
background: yellow;
+
}
}
 +
#team-content{
 +
margin-top: 15px;
 +
margin-bottom: 95px;
 +
}
 +
.team-photo img{
 +
width: 1215px;
 +
}
 +
.team-photo{
 +
margin-bottom: -2px;
 +
}
 +
#top{
 +
background-image: url('https://static.igem.org/mediawiki/2014/8/89/Uestc_top2.png');
 +
width: 65px;
 +
height: 138px;
 +
position: fixed;
 +
top: 70%;
 +
margin-left: 1200px;
 +
cursor: pointer;
 +
z-index: 999;
-
#logo {
 
-
position: relative;
 
-
margin: -35px auto;
 
-
height: 100px;
 
-
line-height:50px;
 
}
}
-
 
+
#logo{
-
#logo a {
+
position: absolute;
-
text-decoration: none;
+
width: 154px;
-
color: #000;
+
height: 147px;
-
text-shadow: 0px 3px 0px #ffffff;
+
margin-left: 1000px;
 +
margin-top: 30px;
 +
cursor: pointer;
}
}
-
 
+
#footer-box{
-
#h1 {
+
display: none;
-
text-transform:Uppercase;
+
-
text-align: left;
+
-
font-size: 2em;
+
-
font-family:"Purisa", cursive;
+
}
}
-
#h2 {
+
.firstHeading{
-
text-transform:Uppercase;
+
display: none;
-
text-align: left;
+
-
font-size: 4em;
+
-
font-family: "Purisa", cursive;
+
}
}
 +
#content{
 +
border:none;
 +
background: none;
 +
}
 +
li{
 +
color:white;
 +
}
 +
#intro1{
 +
font-family: helveticalmd,"微软雅黑";
 +
font-size: 50px;
 +
margin-top: 20px;
 +
margin-left: 10px;
 +
color: white;
 +
}
 +
#intro2{
 +
font-family: helveticalmd,"微软雅黑";
 +
font-size: 23px;
 +
margin-left: 10px;
 +
margin-right: 10px;
 +
color:white;
 +
text-align:justify;
 +
text-justify:inter-ideograph;
-
#main {
+
text-indent:2em;
-
top:130px;
+
-
position: relative;
+
-
padding: 0px;
+
-
margin: 28px 0 0 0;
+
-
width: 1180px;
+
-
color: #474747;
+
}
}
-
 
+
#introduction{
-
#main strong {
+
background-color:#1bbc9b
-
color: #242424;
+
}
}
 +
/*rocket-to-top*/
 +
*{list-style:none;border:0;}
-
#main h2, #main h3, #main h4 {
+
#rocket-to-top div {
-
color: #0d0d0d;
+
    left: 0;
 +
    margin: 0;
 +
    overflow: hidden;
 +
    padding: 0;
 +
    position: absolute;
 +
    top: 0;
 +
    width: 149px;
}
}
-
 
+
#rocket-to-top .level-2 {
-
#main ul {
+
    background: url("https://static.igem.org/mediawiki/2014/a/a6/UestcUp.png") no-repeat scroll -149px 0 transparent;
-
list-style: none;
+
    display: none;
 +
    height: 250px;
 +
    opacity: 0;
 +
    z-index: 1;
}
}
-
 
+
#rocket-to-top .level-3 {
-
#main ul li {
+
    background: none repeat scroll 0 0 transparent;
-
padding: 8px 0 8px 0;
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    <p class="active" style="bottom: 0px;"><span>Our Team: Brilliant Guys</span></p>
 +
        <p style="bottom: -40px;">Plants: Transgenic Tobaccos</p>
 +
        <p style="bottom: -40px;">Super Plant: Plants vs HCOH</p>
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        <span rel="5" class="">5</span>
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<p id="intro1"><b>Introduction</b></p>
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<br/>
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 +
With the rapid society development, a large amount of pollutants are full of our daily life. Nowadays, chemical materials are widely used in our surroundings indoor, such as building materials, paint in new apartments, artificial adhesives in numerous household products and etc, which may lead to excess release of formaldehyde in closed space. Exposing to the substantial formaldehyde may cause irritation, allergic asthma and neurasthenia, as well as carcinogenesis. Nowadays, people mainly get rid of the formaldehyde by increasing the rate of ventilating or using air purifier in those polluted spaces. However, these methods show some limitation, and cannot degrade formaldehyde safely and effectively.
 +
<br/><br/>
 +
To get the inspiration for the design of our project, we did a survey of the knowledge concerning formaldehyde pollution. The results showed that people paid high attention to the indoor formaldehyde pollution, and preferred to adopt a safe and environmental friendly method to decrease the concentration of formaldehyde effectively. A methodology by using green plants got the highest score among all provided approaches. Based on the results of this survey, we decided to use synthetic biology strategy to design a super plant that can remove formaldehyde safely and effectively. 
 +
<br/><br/>
 +
After doing sufficient literature research, we got to know that there is a formaldehyde-fixation pathway in methylotrophs which is called ribulose monophosphate (RuMP) pathway. HPS and PHI are the two key enzymes in this pathway. Besides, there is a formaldehyde folate-independent pathway in some certain plant species. FALDH and FDH play an important role in this pathway. In our 2014 iGEM project, these two pathways were integrated through the methodology of synthetic biology. For this, formaldehyde metabolism related genes from both methylotrophs and plants were transferred into tobacco to rebuild a new formaldehyde metabolism pathway in our super plant.
 +
<br/><br/>
 +
To further increase the degradation efficiency of formaldehyde, we co-expressed gene <i>AHA2</i>, which is original from <i>Arabidopsis thaliana</i>, to regulate stomata opening. In addition, by taking into account of biosafety of genetically modified organism, we introduced the gene of cysteine protease into our super plants. The transgenic tobacco pollen will be aborted while cysteine protease gene is specifically expressed in tobacco tapetum.
 +
<br/><br/>
 +
With more and more achievements have been harvested about the super plant, we organized various activities such as questionnaire survey, school seminar series, video making, etc, to vigorously promote synthetic biology, iGEM and our super plant. Along with the happy summer vacation, we had completed our project successfully with the efforts of all members. Let's meet at MIT and share each other's achievenments.
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isNaN(a=parseFloat(b))?!b||b==="auto"?0:b:a},custom:function(a,c,d){function h(a){return e.step(a)}var e=this,g=f.fx;this.startTime=cq||cr(),this.end=c,this.now=this.start=a,this.pos=this.state=0,this.unit=d||this.unit||(f.cssNumber[this.prop]?"":"px"),h.queue=this.options.queue,h.elem=this.elem,h.saveState=function(){f._data(e.elem,"fxshow"+e.prop)===b&&(e.options.hide?f._data(e.elem,"fxshow"+e.prop,e.start):e.options.show&&f._data(e.elem,"fxshow"+e.prop,e.end))},h()&&f.timers.push(h)&&!co&&(co=setInterval(g.tick,g.interval))},show:function(){var a=f._data(this.elem,"fxshow"+this.prop);this.options.orig[this.prop]=a||f.style(this.elem,this.prop),this.options.show=!0,a!==b?this.custom(this.cur(),a):this.custom(this.prop==="width"||this.prop==="height"?1:0,this.cur()),f(this.elem).show()},hide:function(){this.options.orig[this.prop]=f._data(this.elem,"fxshow"+this.prop)||f.style(this.elem,this.prop),this.options.hide=!0,this.custom(this.cur(),0)},step:function(a){var b,c,d,e=cq||cr(),g=!0,h=this.elem,i=this.options;if(a||e>=i.duration+this.startTime){this.now=this.end,this.pos=this.state=1,this.update(),i.animatedProperties[this.prop]=!0;for(b in i.animatedProperties)i.animatedProperties[b]!==!0&&(g=!1);if(g){i.overflow!=null&&!f.support.shrinkWrapBlocks&&f.each(["","X","Y"],function(a,b){h.style["overflow"+b]=i.overflow[a]}),i.hide&&f(h).hide();if(i.hide||i.show)for(b in i.animatedProperties)f.style(h,b,i.orig[b]),f.removeData(h,"fxshow"+b,!0),f.removeData(h,"toggle"+b,!0);d=i.complete,d&&(i.complete=!1,d.call(h))}return!1}i.duration==Infinity?this.now=e:(c=e-this.startTime,this.state=c/i.duration,this.pos=f.easing[i.animatedProperties[this.prop]](this.state,c,0,1,i.duration),this.now=this.start+(this.end-this.start)*this.pos),this.update();return!0}},f.extend(f.fx,{tick:function(){var a,b=f.timers,c=0;for(;c<b.length;c++)a=b[c],!a()&&b[c]===a&&b.splice(c--,1);b.length||f.fx.stop()},interval:13,stop:function(){clearInterval(co),co=null},speeds:{slow:600,fast:200,_default:400},step:{opacity:function(a){f.style(a.elem,"opacity",a.now)},_default:function(a){a.elem.style&&a.elem.style[a.prop]!=null?a.elem.style[a.prop]=a.now+a.unit:a.elem[a.prop]=a.now}}}),f.each(cp.concat.apply([],cp),function(a,b){b.indexOf("margin")&&(f.fx.step[b]=function(a){f.style(a.elem,b,Math.max(0,a.now)+a.unit)})}),f.expr&&f.expr.filters&&(f.expr.filters.animated=function(a){return f.grep(f.timers,function(b){return a===b.elem}).length});var cv,cw=/^t(?:able|d|h)$/i,cx=/^(?:body|html)$/i;"getBoundingClientRect"in c.documentElement?cv=function(a,b,c,d){try{d=a.getBoundingClientRect()}catch(e){}if(!d||!f.contains(c,a))return d?{top:d.top,left:d.left}:{top:0,left:0};var g=b.body,h=cy(b),i=c.clientTop||g.clientTop||0,j=c.clientLeft||g.clientLeft||0,k=h.pageYOffset||f.support.boxModel&&c.scrollTop||g.scrollTop,l=h.pageXOffset||f.support.boxModel&&c.scrollLeft||g.scrollLeft,m=d.top+k-i,n=d.left+l-j;return{top:m,left:n}}:cv=function(a,b,c){var d,e=a.offsetParent,g=a,h=b.body,i=b.defaultView,j=i?i.getComputedStyle(a,null):a.currentStyle,k=a.offsetTop,l=a.offsetLeft;while((a=a.parentNode)&&a!==h&&a!==c){if(f.support.fixedPosition&&j.position==="fixed")break;d=i?i.getComputedStyle(a,null):a.currentStyle,k-=a.scrollTop,l-=a.scrollLeft,a===e&&(k+=a.offsetTop,l+=a.offsetLeft,f.support.doesNotAddBorder&&(!f.support.doesAddBorderForTableAndCells||!cw.test(a.nodeName))&&(k+=parseFloat(d.borderTopWidth)||0,l+=parseFloat(d.borderLeftWidth)||0),g=e,e=a.offsetParent),f.support.subtractsBorderForOverflowNotVisible&&d.overflow!=="visible"&&(k+=parseFloat(d.borderTopWidth)||0,l+=parseFloat(d.borderLeftWidth)||0),j=d}if(j.position==="relative"||j.position==="static")k+=h.offsetTop,l+=h.offsetLeft;f.support.fixedPosition&&j.position==="fixed"&&(k+=Math.max(c.scrollTop,h.scrollTop),l+=Math.max(c.scrollLeft,h.scrollLeft));return{top:k,left:l}},f.fn.offset=function(a){if(arguments.length)return a===b?this:this.each(function(b){f.offset.setOffset(this,a,b)});var c=this[0],d=c&&c.ownerDocument;if(!d)return null;if(c===d.body)return f.offset.bodyOffset(c);return cv(c,d,d.documentElement)},f.offset={bodyOffset:function(a){var b=a.offsetTop,c=a.offsetLeft;f.support.doesNotIncludeMarginInBodyOffset&&(b+=parseFloat(f.css(a,"marginTop"))||0,c+=parseFloat(f.css(a,"marginLeft"))||0);return{top:b,left:c}},setOffset:function(a,b,c){var d=f.css(a,"position");d==="static"&&(a.style.position="relative");var e=f(a),g=e.offset(),h=f.css(a,"top"),i=f.css(a,"left"),j=(d==="absolute"||d==="fixed")&&f.inArray("auto",[h,i])>-1,k={},l={},m,n;j?(l=e.position(),m=l.top,n=l.left):(m=parseFloat(h)||0,n=parseFloat(i)||0),f.isFunction(b)&&(b=b.call(a,c,g)),b.top!=null&&(k.top=b.top-g.top+m),b.left!=null&&(k.left=b.left-g.left+n),"using"in b?b.using.call(a,k):e.css(k)}},f.fn.extend({position:function(){if(!this[0])return null;var a=this[0],b=this.offsetParent(),c=this.offset(),d=cx.test(b[0].nodeName)?{top:0,left:0}:b.offset();c.top-=parseFloat(f.css(a,"marginTop"))||0,c.left-=parseFloat(f.css(a,"marginLeft"))||0,d.top+=parseFloat(f.css(b[0],"borderTopWidth"))||0,d.left+=parseFloat(f.css(b[0],"borderLeftWidth"))||0;return{top:c.top-d.top,left:c.left-d.left}},offsetParent:function(){return this.map(function(){var a=this.offsetParent||c.body;while(a&&!cx.test(a.nodeName)&&f.css(a,"position")==="static")a=a.offsetParent;return a})}}),f.each({scrollLeft:"pageXOffset",scrollTop:"pageYOffset"},function(a,c){var d=/Y/.test(c);f.fn[a]=function(e){return f.access(this,function(a,e,g){var h=cy(a);if(g===b)return h?c in h?h[c]:f.support.boxModel&&h.document.documentElement[e]||h.document.body[e]:a[e];h?h.scrollTo(d?f(h).scrollLeft():g,d?g:f(h).scrollTop()):a[e]=g},a,e,arguments.length,null)}}),f.each({Height:"height",Width:"width"},function(a,c){var d="client"+a,e="scroll"+a,g="offset"+a;f.fn["inner"+a]=function(){var a=this[0];return a?a.style?parseFloat(f.css(a,c,"padding")):this[c]():null},f.fn["outer"+a]=function(a){var b=this[0];return b?b.style?parseFloat(f.css(b,c,a?"margin":"border")):this[c]():null},f.fn[c]=function(a){return f.access(this,function(a,c,h){var i,j,k,l;if(f.isWindow(a)){i=a.document,j=i.documentElement[d];return f.support.boxModel&&j||i.body&&i.body[d]||j}if(a.nodeType===9){i=a.documentElement;if(i[d]>=i[e])return i[d];return Math.max(a.body[e],i[e],a.body[g],i[g])}if(h===b){k=f.css(a,c),l=parseFloat(k);return f.isNumeric(l)?l:k}f(a).css(c,h)},c,a,arguments.length,null)}}),a.jQuery=a.$=f,typeof define=="function"&&define.amd&&define.amd.jQuery&&define("jquery",[],function(){return f})})(window);
-
<div id="header">
+
</script>
-
+
<script type="text/javascript">
-
<div id="logo">
+
 
-
<h1 id="h1">
+
$(document).ready(function() {
-
<a href="#" style="">UESTC-China 2014 </a>
+
$(".imageRotation").each(function(){
-
</h1>
+
// 获取有关参数
-
<h1 id="h2">
+
var imageRotation = this,  // 图片轮换容器
-
<a href="#" style="">PLANTS vs HCHO</a>
+
imageBox = $(imageRotation).children(".imageBox")[0],  // 图片容器
-
</h1>
+
titleBox = $(imageRotation).children(".titleBox")[0],  // 标题容器
-
</div>
+
titleArr = $(titleBox).children(),  // 所有标题（数组）
-
<div id="nav">
+
icoBox = $(imageRotation).children(".icoBox")[0],  // 图标容器
-
<ul style="text-transform:Uppercase">
+
icoArr = $(icoBox).children(),  // 所有图标（数组）
-
<li class="first active">
+
imageWidth = $(imageRotation).width(),  // 图片宽度
-
<a href="#">Home</a>
+
imageNum = $(imageBox).children().size(),  // 图片数量
-
</li>
+
imageReelWidth = imageWidth*imageNum,  // 图片容器宽度
-
<li>
+
activeID = parseInt($($(icoBox).children(".active")[0]).attr("rel")),  // 当前图片ID
-
<a href="#">Team</a>
+
nextID = 0,  // 下张图片ID
-
</li>
+
setIntervalID,  // setInterval() 函数ID
-
<li>
+
intervalTime = 4000,  // 间隔时间
-
<a href="#">Porject</a>
+
imageSpeed =500,  // 图片动画执行速度
-
</li>
+
titleSpeed =250;  // 标题动画执行速度
-
<li>
+
// 设置 图片容器 的宽度
-
<a href="#">Model</a>
+
$(imageBox).css({'width' : imageReelWidth + "px"});
-
</li>
+
// 图片轮换函数
-
<li>
+
var rotate=function(clickID){
-
<a href="#">Links</a>
+
if(clickID){ nextID = clickID; }
-
</li>
+
else{ nextID=activeID<=4 ? activeID+1 : 1; }
-
<li>
+
// 交换图标
-
<a href="#">Notebook</a>
+
$(icoArr[activeID-1]).removeClass("active");
-
</li>
+
$(icoArr[nextID-1]).addClass("active");
-
<li>
+
// 交换标题
-
<a href="#">Safety</a>
+
$(titleArr[activeID-1]).animate(
-
</li>
+
{bottom:"-40px"},
-
<li class="last">
+
titleSpeed,
-
<a href="#">Human Practice</a>
+
function(){
-
</li>
+
$(titleArr[nextID-1]).animate({bottom:"0px"} , titleSpeed);
-
</ul>
+
}
-
<br class="clear" />
+
);
-
</div>
+
// 交换图片
-
</div>
+
$(imageBox).animate({left:"-"+(nextID-1)*imageWidth+"px"} , imageSpeed);
-
<div id="banner">
+
// 交换IP
-
<img src="images/pic1.jpg" width="1124" height="212" alt="" />
+
activeID = nextID;
-
</div>
+
}
-
<div id="main">
+
setIntervalID=setInterval(rotate,intervalTime);
-
<div id="sidebar1">
+
$(imageBox).hover(
-
<h3>
+
function(){ clearInterval(setIntervalID); },
-
Pharetra sodales egestas
+
function(){ setIntervalID=setInterval(rotate,intervalTime); }
-
</h3>
+
);
-
<p>
+
$(icoArr).click(function(){
-
Enim amet sociis ullamcorper lorem ligula. Viverra odio massa sapien et augue lacus pretium.  
+
clearInterval(setIntervalID);
-
Sociis magna faucibus dictum curabitur auctor et molestie proin sed aliquam.
+
var clickID = parseInt($(this).attr("rel"));
-
</p>
+
rotate(clickID);
-
<h3>
+
setIntervalID=setInterval(rotate,intervalTime);
-
Venenatis elementum porttitor
+
});
-
</h3>
+
});
-
<ul class="linkedList">
+
});
-
<li class="first">
+
</script>
-
<a href="#">Varius nulla fusce suscipit</a>
+
<script type="text/javascript">
-
</li>
+
 
-
<li>
+
$(document).ready(function(){
-
<a href="#">Rutrum orci amet et lorem</a>
+
 
-
</li>
+
var imageload=new Array("https://static.igem.org/mediawiki/2014/1/15/NewPlant1.jpg","https://static.igem.org/mediawiki/2014/8/83/Home1.jpg","https://static.igem.org/mediawiki/2014/9/9f/NewPlant2.jpg","https://static.igem.org/mediawiki/2014/1/14/All_students3.jpg");
-
<li>
+
var datatemp=function(){
-
<a href="#">Convallis sodales malesuada</a>
+
this.head;
-
</li>
+
this.content;
-
<li>
+
}
-
<a href="#">Faucibus imperdiet adipiscing</a>
+
var data1=new datatemp();
-
</li>
+
var data2=new datatemp();
-
<li>
+
var data3=new datatemp();
-
<a href="#">Diam porta at sed tortor</a>
+
var data4=new datatemp();
-
</li>
+
data1.head="Plants";
-
<li>
+
data1.content="Transgenic Tobaccos";
-
<a href="#">Rutrum orci amet et lorem</a>
+
data2.head="SuperPlant ";
-
</li>
+
data2.content="Plants vs HCOH";
-
<li>
+
data3.head="Plants ";
-
<a href="#">Convallis sodales malesuada</a>
+
data3.content="Transform";
-
</li>
+
data4.head="Our Team";
-
<li>
+
data4.content="Keep moving";
-
<a href="#">Faucibus imperdiet adipiscing</a>
+
var imageintro=new Array(data1,data2,data3,data4);
-
</li>
+
var imagecount=0;
-
<li class="last">
+
-
<a href="#">Dictum in amet phasellus</a>
+
var init=function(){
-
</li>
+
$('.arrow').hide();
-
</ul>
+
$('.top-menu-sub').hide();
-
</div>
+
 
-
<div id="sidebar2">
+
}
-
<h3>
+
var bind=function(){
-
Dictum sed montes
+
$("#top").hide();
-
</h3>
+
$(document).scroll(function(){
-
<ul class="linkedList">
+
if($(document).scrollTop()>308){
-
<li class="first">
+
$("#left-bar").css("position","fixed");
-
<a href="#">Euismod parturient dolor</a>
+
$("#left-bar").css("top","0%");
-
</li>
+
}
-
<li>
+
else{
-
<a href="#">Malesuada dignissim aenean</a>
+
console.log(1);
-
</li>
+
$("#left-bar").css("position","absolute");
-
<li>
+
$("#left-bar").css("top","");
-
<a href="#">Felis amet condimentum</a>
+
 
-
</li>
+
};
-
<li>
+
})
-
<a href="#">Duis rutrum urna congue</a>
+
$('.top-menu-each').hover(function(){
-
</li>
+
$('.top-menu-each.click .arrow').hide();
-
<li>
+
$('.top-menu-each.click').removeClass('click');
-
<a href="#">Iaculis etiam sed nibh</a>
+
$(this).addClass('click');
-
</li>
+
$(this).find('.arrow').show();
-
<li class="last">
+
$('.top-menu-sub.show').hide();
-
<a href="#">Metus dolor et consequat</a>
+
$('.top-menu-sub.show').removeClass('show');
-
</li>
+
$(this).find('.top-menu-sub').addClass('show');
-
</ul>
+
$(this).find('.top-menu-sub').show();}
-
<h3>
+
,
-
Nulla parturient tortor
+
function() {
-
</h3><img class="top" src="images/pic1.jpg" width="232" height="84" alt="" />
+
 
-
<p>
+
$(this).removeClass('click');
-
Facilisis risus tortor elit scelerisque integer nascetur. Magnis lacus arcu erat gravida diam lorem et posuere.  
+
$(this).find('.arrow').hide();
-
Aliquam proin nisl mus ultrices morbi consequat veroeros dolore.
+
$(this).find('.top-menu-sub').removeClass('show');
-
</p>
+
$(this).find('.top-menu-sub').hide();
-
</div>
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Latest revision as of 15:14, 17 October 2014

Plant Vs HCHO

Our Team: Brilliant Guys

Plants: Transgenic Tobaccos

Super Plant: Plants vs HCOH

Safety: Operation

Human Practice

1 2 3 4 5

Introduction


With the rapid society development, a large amount of pollutants are full of our daily life. Nowadays, chemical materials are widely used in our surroundings indoor, such as building materials, paint in new apartments, artificial adhesives in numerous household products and etc, which may lead to excess release of formaldehyde in closed space. Exposing to the substantial formaldehyde may cause irritation, allergic asthma and neurasthenia, as well as carcinogenesis. Nowadays, people mainly get rid of the formaldehyde by increasing the rate of ventilating or using air purifier in those polluted spaces. However, these methods show some limitation, and cannot degrade formaldehyde safely and effectively.

To get the inspiration for the design of our project, we did a survey of the knowledge concerning formaldehyde pollution. The results showed that people paid high attention to the indoor formaldehyde pollution, and preferred to adopt a safe and environmental friendly method to decrease the concentration of formaldehyde effectively. A methodology by using green plants got the highest score among all provided approaches. Based on the results of this survey, we decided to use synthetic biology strategy to design a super plant that can remove formaldehyde safely and effectively.

After doing sufficient literature research, we got to know that there is a formaldehyde-fixation pathway in methylotrophs which is called ribulose monophosphate (RuMP) pathway. HPS and PHI are the two key enzymes in this pathway. Besides, there is a formaldehyde folate-independent pathway in some certain plant species. FALDH and FDH play an important role in this pathway. In our 2014 iGEM project, these two pathways were integrated through the methodology of synthetic biology. For this, formaldehyde metabolism related genes from both methylotrophs and plants were transferred into tobacco to rebuild a new formaldehyde metabolism pathway in our super plant.

To further increase the degradation efficiency of formaldehyde, we co-expressed gene AHA2, which is original from Arabidopsis thaliana, to regulate stomata opening. In addition, by taking into account of biosafety of genetically modified organism, we introduced the gene of cysteine protease into our super plants. The transgenic tobacco pollen will be aborted while cysteine protease gene is specifically expressed in tobacco tapetum.

With more and more achievements have been harvested about the super plant, we organized various activities such as questionnaire survey, school seminar series, video making, etc, to vigorously promote synthetic biology, iGEM and our super plant. Along with the happy summer vacation, we had completed our project successfully with the efforts of all members. Let's meet at MIT and share each other's achievenments.

pic

Retrieved from "http://2014.igem.org/Team:UESTC-China"