Team:Evry/Interlab Study/08-22-2014

From 2014.igem.org

(Difference between revisions)
 
(9 intermediate revisions not shown)
Line 5: Line 5:
<html>
<html>
     <div class="cd-timeline-content">
     <div class="cd-timeline-content">
 +
<h2>Contructions PSB1C3 with I20260, J23101-E1010 and K823012-E1010</h2>
       <p align="justify">
       <p align="justify">
<br/> Transformation plate observation:
<br/> Transformation plate observation:
-
<br/>- BBa_J23101: nothing grown
+
<br/>- BBa_J23101: 11 isolated colonies
-
<br/>- BBa_J23115 (K823012): 100-150 isolated colonies
+
<br/>- BBa_K823012: 100-150 isolated colonies
<br/>
<br/>
-
<br/> A PCR was performed on 8 colonies for BBa_J23115 (K823012) following the protocol Table 3 and 4.
+
<br/> A PCR was performed with 8 colonies for BBa_K823012 and for BBa_J23101 following the protocol Table 3 and 4.
<br/> To verify PCR products, 10 µl was loaded on a 1% agarose gel with 2 µl of loading dye 6X.
<br/> To verify PCR products, 10 µl was loaded on a 1% agarose gel with 2 µl of loading dye 6X.
Line 16: Line 17:
  <div class="thumb tnone">
  <div class="thumb tnone">
   <div class="thumbinner" style="width:502px;">
   <div class="thumbinner" style="width:502px;">
-
   <a href="URL IMAGE" class="image">
+
   <a href="https://static.igem.org/mediawiki/2014/2/28/22082014gel1-2_-_Copie.jpg" class="image">
-
     <img alt="IMAGE" src="URL IMAGE" width="500px;" class="thumbimage"/>
+
     <img alt="IMAGE" src="https://static.igem.org/mediawiki/2014/2/28/22082014gel1-2_-_Copie.jpg" width="300px;" class="thumbimage"/>
   </a>
   </a>
   <div class="thumbcaption">
   <div class="thumbcaption">
     <div class="magnify">
     <div class="magnify">
-
     <a href="URL IMAGE" class="internal" title="Enlarge">
+
     <a href="https://static.igem.org/mediawiki/2014/2/28/22082014gel1-2_-_Copie.jpg" class="internal" title="Enlarge">
       <img src="/wiki/skins/common/images/magnify-clip.png" width="15" height="11" alt="Symbol"/>
       <img src="/wiki/skins/common/images/magnify-clip.png" width="15" height="11" alt="Symbol"/>
     </a>
     </a>
     </div>
     </div>
-
     <center>1% agarose gel of PCR products of the eight selected colonies</center>
+
     <center>1% agarose gel of PCR products. Lane 1 to 8: BBa_K823012 PCR products and Lane 9: Purple 2-Log. ladder NEB</center>
   </div>
   </div>
   </div>
   </div>
Line 31: Line 32:
</div>
</div>
-
<br/> We expected to obtain a band between 300 and 400 bp.
+
<br/> We expected to obtain a band between 300 and 400 bp. So we decided to amplify the colony n°8. The colony was seed in 5 ml of LB medium and 5 µl of chloramphenicol. Culture was incubated overnight at 37°C with 200 rpm agitation.
-
 
+
<div class="center">
 +
<div class="thumb tnone">
 +
  <div class="thumbinner" style="width:502px;">
 +
  <a href="https://static.igem.org/mediawiki/2014/d/d5/Gel22082014bis.jpg" class="image">
 +
    <img alt="IMAGE" src="https://static.igem.org/mediawiki/2014/d/d5/Gel22082014bis.jpg" width="500px;" class="thumbimage"/>
 +
  </a>
 +
  <div class="thumbcaption">
 +
    <div class="magnify">
 +
    <a href="https://static.igem.org/mediawiki/2014/d/d5/Gel22082014bis.jpg" class="internal" title="Enlarge">
 +
      <img src="/wiki/skins/common/images/magnify-clip.png" width="15" height="11" alt="Symbol"/>
 +
    </a>
 +
    </div>
 +
    <center>1% agarose gel of PCR products. Lane 10 to 17: BBa_J23101 PCR products and Lane 9: Purple 2-Log ladder NEB</center>
 +
  </div>
 +
  </div>
 +
</div>
 +
</div>
 +
<br/>
 +
<br/> We expected to obtain a band around 1200 bp. So we decided to amplify the colony n°2 corresponding to lane 11. The colony was seed in 5 ml of LB medium and 5 µl of chloramphenicol. Culture was incubated overnight at 37°C with 200 rpm agitation.
</p>
</p>

Latest revision as of 17:59, 19 September 2014

Picture

Contructions PSB1C3 with I20260, J23101-E1010 and K823012-E1010


Transformation plate observation:
- BBa_J23101: 11 isolated colonies
- BBa_K823012: 100-150 isolated colonies

A PCR was performed with 8 colonies for BBa_K823012 and for BBa_J23101 following the protocol Table 3 and 4.
To verify PCR products, 10 µl was loaded on a 1% agarose gel with 2 µl of loading dye 6X.

IMAGE
Symbol
1% agarose gel of PCR products. Lane 1 to 8: BBa_K823012 PCR products and Lane 9: Purple 2-Log. ladder NEB

We expected to obtain a band between 300 and 400 bp. So we decided to amplify the colony n°8. The colony was seed in 5 ml of LB medium and 5 µl of chloramphenicol. Culture was incubated overnight at 37°C with 200 rpm agitation.
IMAGE
Symbol
1% agarose gel of PCR products. Lane 10 to 17: BBa_J23101 PCR products and Lane 9: Purple 2-Log ladder NEB


We expected to obtain a band around 1200 bp. So we decided to amplify the colony n°2 corresponding to lane 11. The colony was seed in 5 ml of LB medium and 5 µl of chloramphenicol. Culture was incubated overnight at 37°C with 200 rpm agitation.

Aug 22

Retrieved from "http://2014.igem.org/Team:Evry/Interlab_Study/08-22-2014"