Team:Evry/Notebook/Sensing/PCBs/08-13-2014

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<u>Sensor construction HbpR/PhbpC/HbpC:</u>
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<b><u>Survival test on E.coli BL21:</u></b>
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<br/>10mg of 2 hydroxy,3'-4'dichlorobiphenyl have been received. The compound was lyophilized so it's has been dissolved in 17mL of DMSO in order to have a final concentration of 10(-2)M
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<li>Plate of DH5a which contains pHYB130 plasmid with HbpR-PhbpC-HbpC sequence on LB-agar-Amp
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<br/>The compound was stored at -20°C, in the dark because the DMSO is sensitive to the light.
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<li>Incubation at 37°C overnight
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Latest revision as of 10:41, 6 September 2014

Picture


Survival test on E.coli BL21:
10mg of 2 hydroxy,3'-4'dichlorobiphenyl have been received. The compound was lyophilized so it's has been dissolved in 17mL of DMSO in order to have a final concentration of 10(-2)M
The compound was stored at -20°C, in the dark because the DMSO is sensitive to the light.

Aug 13