Team:UFMG Brazil

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<h1 >WIKI UNDER CONSTRUCTION</h1>
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<p>Hello guys!! This year our team are proposing a project for another serious disease that thousands of people are
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  affected every year in the whole world:<br>
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  We intend to help in the colon cancer diagnosis!
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<br>
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<a href="https://2014.igem.org/Team:UFMG_Brazil"style="color:#000000"></a></p>
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<p style="color:#E7E7E7"> <a href="https://2014.igem.org/wiki/index.php?title=Team:UFMG_Brazil&action=edit"style="color:#000000"> Click here  to edit this page!</a> </p>
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                            HEADER
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                                <li class="current"><a href="https://2014.igem.org/Team:UFMG_Brazil">Home</a></li>
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                                <li><a href="https://2014.igem.org/Team:UFMG_Brazil/Team">Team</a></li>
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                                <li><a href="https://2014.igem.org/Team:UFMG_Brazil/Safety">Safety</a></li>
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                                <li><a href="#">Project</a>
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                                    <ul style="display:none;">
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                                        <li><a href="https://2014.igem.org/Team:UFMG Brazil/Project/Intro">Introduction</a></li>
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                                        <li><a href="https://2014.igem.org/Team:UFMG_Brazil/Project/Parts">Parts</a></li>
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                                        <li><a href="https://2014.igem.org/Team:UFMG_Brazil/Project/Protocols">Protocols and results</a></li>
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                                        <li><a href="https://2014.igem.org/Team:UFMG_Brazil/Project/Modelling">Modelling</a></li>
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                                        <li><a href="https://2014.igem.org/Team:UFMG_Brazil/Project/Policy">Policy and practices</a></li>
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                                        <li><a href="https://2014.igem.org/Team:UFMG_Brazil/Project/Notebook">Notebook</a></li>
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                                </li>
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                                <li><a href="#">Thanks to</a>
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                                      <li>
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                                        <a href="https://2014.igem.org/Team:UFMG_Brazil/sponsors">Sponsors</a>
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                                      </li>
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                                        <a href="https://2014.igem.org/Team:UFMG_Brazil/attributions">Attributions</a>
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                    <div class="unit" style="padding-top:20px; padding-bottom: 20px;">
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                      <div class="title1">- Team UFMG Brazil presents -</div>
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                    </div>
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                    <div class="slogan">How to stop a war<br>before it starts?</div>
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                    <div class="unit" style="padding-top:10px;">
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                      <div class="" style="padding-bottom:10px;"><img src="https://static.igem.org/mediawiki/2014/7/77/Logooverlay.png" alt=""></div>
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                      <div class="title1">Come closer and discover</div>
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                      <div class="title2" style="color: #eae6e3;">The Colon Yeast</div>
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                      <div>
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                          <p style="padding-top: 28px;">The hemiCherry in the (FAECAL) Cake</p>
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                          <img src="https://static.igem.org/mediawiki/2014/4/4b/Cherry1.png" alt="">
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                      <!--
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                      <p><time datetime="2014-01-11">wednesday, may 28</time>
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                      &nbsp;|&nbsp;Winning Numbers:</p>
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                      <ul class="list">
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                          <li>19</li>
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                          <li>24</li>
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                          <li>45</li>
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                          <li>18</li>
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                          <li>36</li>
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                          <li>5</li>
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                      </ul>
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                      <div class="ticket">
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                          <a href="#">see current winning <br>ticket results</a>
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                            CONTENT
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                            <h3>So, how to stop a war before it started?</h3>
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                            <br>
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                            <p style="text-transform: none;"><span>Colorectal cancer includes tumors affecting a segment of the large intestine (colon) and rectum. It is treatable and, in most cases, curable when detected early, before it hasn't spread to other organs. <br><br>However, the diagnosis requires a biopsy, made ​​by handset introduced via rectum. Noticeably, this procedure is highly invasive and often inhibits patients to submit to this practice, hampering the discovery of cancer at an early stage and thereby reducing the chances of effective treatment and cure. On our institution, a modified probiotic strain of Saccharomyces cerevisiae was isolated from local beverage.<br><br>Our objective is to introduce this strain as a new probiotic chassis on iGEM, and also to develop a gene circuit that allows it to detect one or more stool biomarkers for colorectal cancer on the intestine and report with a color change on the feces. We chose L-DNA (long DNA molecules) as biomarker, and designed a chimeric protein using TALE DNA-Binding Domains and a split version of mCherry.<br><br> This protein can detect certain repetitive sequences on DNA and emit red fluorescence, which when measured serve as proxy for the DNA size. Since RFP emission falls on the visible spectrum, it can also be used for staining the feces, allowing for a patient consuming the probiotic to become aware of the possibility of the developing problem just by checking on the color of their stool.</span></p>
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                            <h2>Believe in your win! <span>Good luck!</span></h2>
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                            <div class="grid_4 alpha">
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                                <a href="#" class="link1">How To Play</a>
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                                <p>Praesent vestibulum aenean nonummy hendrerit mauris. Hasellus porta. Fusce suscipit varius mi. Cum sociis atoque penatibus magnis dis parturient montes nascetur ridiculus mus.</p>
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                            <div class="grid_4 omega">
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                                <ul class="links">
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                                    <li><a href="#" class="col1">for <br>players</a>
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                                    </li>
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                                    <li><a href="#" class="col2">for <br>retailers</a>
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                                    <li><a href="#" class="col3">message <br>center</a>
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<a href="https://2014.igem.org/Team:UFMG_Brazil"style="color:#000000">Home </a> </td>
 
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<a href="https://2014.igem.org/Team:UFMG_Brazil/Project"style="color:#000000"> Project</a></td>
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                        <h2>latest winners:</h2>
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                                  <div class="caption">
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                                      <a href="#" title="">Richard Clark</a>
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                                      <p>Won $1.000 <span>playing <br>$1000 overload</span></p>
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                                      <a href="#" title="">Tamara Smith</a>
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                                      <p>Won $1.000 <span>playing <br>$1000 overload</span></p>
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                                  </div>
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                                      <a href="#" title="">Linda Nelson</a>
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                                      <p>Won $1.000 <span>playing <br>$1000 overload</span></p>
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                                  </div>
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                                      <a href="#" title="">George Johnson</a>
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                                      <p>Won $1.000 <span>playing <br>$1000 overload</span></p>
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                                  </div>
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                              </div>
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                                  <div class="caption">
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                                      <a href="#" title="">Richard Clark</a>
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                                      <p>Won $1.000 <span>playing <br>$1000 overload</span></p>
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                                      <a href="#" title="">Tamara Smith</a>
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                                      <p>Won $1.000 <span>playing <br>$1000 overload</span></p>
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                        <h4>We are from Brazil!</h4>
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                        <p>aaa aaa</p>
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                      <span>|</span>
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                      <span><a href="https://2014.igem.org/Team:UFMG_Brazil">Team UFMG Brazil</a></span>
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<tr><td colspan="3"> <h3><strong id="docs-internal-guid-7635cd64-d633-19e2-7369-0a1a982a08ff">The Colonyeast – Project Description</strong></h3>
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<p dir="ltr">Colorectal cancer includes tumors affecting a segment of the large intestine (colon) and rectum. It is treatable and, in most cases, curable when detected early, before it hasn&rsquo;t spread to other organs. However, the diagnosis requires a biopsy, made ​​by handset introduced via rectum. Noticeably, this procedure is highly invasive and often inhibits patients to submit to this practice, hampering the discovery of cancer at an early stage and thereby reducing the chances of effective treatment and cure.</p>
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<p dir="ltr">On our institution, a modified probiotic strain of Saccharomyces cerevisiae was isolated from local beverage. Our objective is to introduce this strain as a new probiotic chassis on iGEM, and also to develop a gene circuit that allows it to detect one or more stool biomarkers for colorectal cancer on the intestine and report with a color change on the feces. We chose L-DNA (long DNA molecules) as biomarker, and designed a chimeric protein using TALE DNA-Binding Domains and a split version of mCherry. This protein can detect certain repetitive sequences on DNA and emit red fluorescence, which when measured serve as proxy for the DNA size. Since RFP emission falls on the visible spectrum, it can also be used for staining the feces, allowing for a patient consuming the probiotic to become aware of the possibility of the developing problem just by checking on the color of their stool.</p>
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Latest revision as of 17:44, 14 June 2015

Home UFMG Team

So, how to stop a war before it started?


Colorectal cancer includes tumors affecting a segment of the large intestine (colon) and rectum. It is treatable and, in most cases, curable when detected early, before it hasn't spread to other organs.

However, the diagnosis requires a biopsy, made ​​by handset introduced via rectum. Noticeably, this procedure is highly invasive and often inhibits patients to submit to this practice, hampering the discovery of cancer at an early stage and thereby reducing the chances of effective treatment and cure. On our institution, a modified probiotic strain of Saccharomyces cerevisiae was isolated from local beverage.

Our objective is to introduce this strain as a new probiotic chassis on iGEM, and also to develop a gene circuit that allows it to detect one or more stool biomarkers for colorectal cancer on the intestine and report with a color change on the feces. We chose L-DNA (long DNA molecules) as biomarker, and designed a chimeric protein using TALE DNA-Binding Domains and a split version of mCherry.

This protein can detect certain repetitive sequences on DNA and emit red fluorescence, which when measured serve as proxy for the DNA size. Since RFP emission falls on the visible spectrum, it can also be used for staining the feces, allowing for a patient consuming the probiotic to become aware of the possibility of the developing problem just by checking on the color of their stool.

We are from Brazil!

aaa aaa

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