Team:Evry/Notebook/CellCharacterization/Antibiotic test

From 2014.igem.org

(Difference between revisions)
 
(44 intermediate revisions not shown)
Line 1: Line 1:
 +
<html>
 +
<div align="center">
 +
<h4 class=title4> <FONT COLOR="blue">Preparation of antibiotic stocks </FONT></h4>
 +
</div>
-
 
-
<html>
 
-
  <div class="cd-timeline-block">
 
-
</html>
 
-
{{:Team:Evry/Notebook/Biology/TopImage}}
 
-
<html>
 
-
    <div class="cd-timeline-content">
 
-
<h4 class=title4> <u> Preparation of antibiotic stocks </u> </h4>
 
<p>
<p>
-
<table>
+
<table border="1">
<tr >
<tr >
-
 
+
   <td> <b><FONT COLOR=#000033>Antibiotic</font> </b> </td>
-
   <td> <b>Antibiotic </b> </td>
+
   <td> <b><FONT COLOR=#000033>Stock concentration</font> </b> </td>
-
   <td> <b>Stock concentration </b> </td>
+
   <td class= table> <b><FONT COLOR=#000033>Protocol </font></b> </td>
-
   <td class= table> <b>Protocol </b> </td>
+
</tr>
</tr>
Line 22: Line 17:
   <td class= conc> 25 mg/mL </td>
   <td class= conc> 25 mg/mL </td>
   <td class= proto> Weight 0.56g of Kanamycin powder, solubilization into 20mL of miliQ water. <br>Vortex 5min and filtration with a 200nm filter. </br>  Stock : -20°C (aliquots of 1mL) <br> </br></td>
   <td class= proto> Weight 0.56g of Kanamycin powder, solubilization into 20mL of miliQ water. <br>Vortex 5min and filtration with a 200nm filter. </br>  Stock : -20°C (aliquots of 1mL) <br> </br></td>
-
 
</tr>
</tr>
Line 32: Line 26:
<tr>
<tr>
   <td> Amplicilin </td>
   <td> Amplicilin </td>
-
   <td class= conc> 50 mg/mL </td>
+
   <td class= conc> 100 mg/mL </td>
-
   <td class= proto> Weight 1g of Amplicilin powder, solubilization into 40mL of miliQ water. <br>Vortex 5min and filtration with a 200nm filter. </br>  Stock : -20°C (aliquots of 1mL) <br> </br> </td>
+
   <td class= proto> Weight 1g of Amplicilin powder, solubilization into 20mL of miliQ water. <br>Vortex 5min and filtration with a 200nm filter. </br>  Stock : -20°C (aliquots of 1mL) <br> </br> </td>
</tr>
</tr>
<tr>
<tr>
Line 45: Line 39:
   <td class= proto> Weight 0.34g of Chloramphenicol powder, solubilization into 10mL of ethanol 100%. <br> Vortex 5min and filtration with a 200nm filter. </br>  Stock : -20°C and hiden from light(aliquots of 1mL) <br> </br> </td>
   <td class= proto> Weight 0.34g of Chloramphenicol powder, solubilization into 10mL of ethanol 100%. <br> Vortex 5min and filtration with a 200nm filter. </br>  Stock : -20°C and hiden from light(aliquots of 1mL) <br> </br> </td>
</tr>
</tr>
-
 
</table>
</table>
Line 51: Line 44:
</p>
</p>
-
 
-
<span class="cd-date"></span>
 
-
</div>
 
-
</div>
 
</html>
</html>
-
 
-
 
-
<!--<html>
 
-
<head>
 
-
</head>
 
-
<body>
 
-
<style type="text/css">.table { text-align: center} .conc {width: 130px} .title2 { text-align: center; font-family: calibri } .title4 {  font-family: calibri } .proto{width: 600px} td{width: 130px}</style>
 
-
<div class="week">
 
-
  <h2 class=title2> <b> Antibiotic test </b></h2>
 
-
  <div class="CellCharacterization">
 
-
 
-
 
-
  </div>
 
-
  </div>
 
-
</body>
 
-
</html>-->
 

Latest revision as of 10:40, 17 October 2014

Preparation of antibiotic stocks

Antibiotic Stock concentration Protocol
Kanamycin 25 mg/mL Weight 0.56g of Kanamycin powder, solubilization into 20mL of miliQ water.
Vortex 5min and filtration with a 200nm filter.
Stock : -20°C (aliquots of 1mL)

Streptomycin 100 mg/mL Weight 1g of Streptomycin powder, solubilization into 20mL of miliQ water.
Vortex 5min and filtration with a 200nm filter.
Stock : -20°C (aliquots of 1mL)

Amplicilin 100 mg/mL Weight 1g of Amplicilin powder, solubilization into 20mL of miliQ water.
Vortex 5min and filtration with a 200nm filter.
Stock : -20°C (aliquots of 1mL)

Tetracyclin 15 mg/mL Weight 0.6g of Tetracyclin powder, solubilization into 20mL of ethanol 50%.
Vortex 5min and filtration with a 200nm filter.
Stock : -20°C and hiden from light(aliquots of 1mL)
Chloramphenicol 34 mg/mL Weight 0.34g of Chloramphenicol powder, solubilization into 10mL of ethanol 100%.
Vortex 5min and filtration with a 200nm filter.
Stock : -20°C and hiden from light(aliquots of 1mL)

Retrieved from "http://2014.igem.org/Team:Evry/Notebook/CellCharacterization/Antibiotic_test"