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Team:UNIK Copenhagen/Logbook
From 2014.igem.org
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| - | <p><b> | + | <p><b>17<sup>th</sup> of July - amplification of pETDuet-1 (Q)</b><br> |
| - | + | Transformation of Ecloni with pETDuet-1 vector and plated the bacteria. They were afterwards stored in the fridge.<br><br> | |
| - | <b> | + | <b>23<sup>rd</sup> of July (Q)</b><br>We made three liquid cultures each from a single colony on the plate.<br><br> |
| - | <b> | + | <b>24<sup>th</sup> of July(Q)</b><br>Minipreps of the pETDuet-1 vector. The minipreps was nanodroped. The measurements were:<br></p> |
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| - | <p>On basis of the measurements, we picked sample 2 for sequencing. The sequences confirmed by sequencing.</p> | + | <p>On basis of the measurements, we picked sample 2 for sequencing. The sequences confirmed by sequencing.<br><br> |
| + | <b>20<sup>st</sup> of August (Q)</b><br> | ||
| + | Ecloni was transformed with pUC57 containing sequences for either Estradiol light chain (EL) or Estradiol heavy chain (EH). The same was done for Progesterone light chain (PL) and heavy chain (PH). We also ran a PCR on pUC57, EL, EH, PL and PH.</p> | ||
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Latest revision as of 13:23, 22 August 2014
17th of July - amplification of pETDuet-1 (Q)
Transformation of Ecloni with pETDuet-1 vector and plated the bacteria. They were afterwards stored in the fridge.
23rd of July (Q)
We made three liquid cultures each from a single colony on the plate.
24th of July(Q)
Minipreps of the pETDuet-1 vector. The minipreps was nanodroped. The measurements were:
| Colony: | ng/µl | 260/280 | 260/230 |
| 1 | 30.3 | 1.96 | 2.34 |
| 2 | 63.3 | 1.82 | 1.89 |
| 3 | 32.1 | 1.82 | 1.68 |
On basis of the measurements, we picked sample 2 for sequencing. The sequences confirmed by sequencing.
20st of August (Q)
Ecloni was transformed with pUC57 containing sequences for either Estradiol light chain (EL) or Estradiol heavy chain (EH). The same was done for Progesterone light chain (PL) and heavy chain (PH). We also ran a PCR on pUC57, EL, EH, PL and PH.
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