Team:Cambridge-JIC/Enhancer Trap

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<h2> Enhancer Trap </h2>
<h2> Enhancer Trap </h2>
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<p> Text here and so on </p>
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<h3> Prologue </h3>
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<p>As a relatively new chassis for synthetic biology, <Em>Marchantia polymorpha</Em> has few promoters and enhancers. The viral promoter '35S' and the '?' are the only highly tested promoters for each. Consequently, we are randomly integrating a plasmid with a minimal promoter driving a GFP gene into the <Em>Marchantia polymorpha</Em> genome, and observing expression levels. After screening several thousand transformants for sex-specific/developmental-specific/highly strong promoters, we will reverse PCR several kb upstream from the inserted genes. These shall then be released as parts, if successful.</p>
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<h3> Lab Notebook </h3>
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<Em>Objective: randomly integrate a plasmid with a minimal promoter into the Marchantia genome, using a Venus flo-protein reporter. Then screen, and establish enhancer lines which are tissue or developmental stage specific, or of great strength.
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</Em>
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Latest revision as of 15:12, 22 August 2014