Team:Arizona State

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<h1 >ARIZONA STATE UNIVERSITY</h1>
 
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<p>The Arizona State University iGEM team has taken on a project of creating biodiesel in a new and efficient way using Escherichia coli. Using a wax esterase, fatty acids and ethanol, which are both naturally produced by E. coli, can be combined to form biodiesel. However, E. coli uses the same intermediate products to produce both fatty acids and ethanol, which reduces the efficiency of this process. This problem can be avoided by engineering two different strains of E. coli to work in tandem. One strain will be focused on producing free fatty acids (FFA) through increased production of enzymes such as TesA and acc. The other strain will be focused on producing ethanol through the increased production of enzymes such as pdc and adhB. These two products can be combined in one of the two cells using a waxy esterase, producing the final biodiesel product. This form of cooperative production will hopefully produce more ethanol than current single strain methods using E. coli.</p>
 
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<p style="color:#E7E7E7"> <a href="https://2014.igem.org/wiki/index.php?title=Team:Arizona_State&action=edit"style="color:#FFFFFF"> Click here  to edit this page!</a> </p>
 
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<tr> <td colspan="9"><a href="https://2014.igem.org/Main_Page" style="width:65px; float:right; margin-left:5px"> <img src="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png" width="55px"></a>
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  <li><a href="https://2014.igem.org/Team:Arizona_State">home </a></li>
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  <li><a href="https://2014.igem.org/Team:Arizona_State/team">team </a>
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      <ul><li><a href="https://2014.igem.org/Team:Arizona_State/members">members </a></li>
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        <li><a href="https://igem.org/Team.cgi?id=1502">iGEM profile </a></li></ul></li>
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  <li><a href="https://2014.igem.org/Team:Arizona_State/project">project </a>
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      <ul><li><a href="https://2014.igem.org/Team:Arizona_State/science">science</a></li>
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        <li><a href="https://2014.igem.org/Team:Arizona_State/policypractices">policy and practices</a></li></ul></li>
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  <li><a href="https://2014.igem.org/Team:Arizona_State/parts">parts </a></li>
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  <li><a href="https://2014.igem.org/Team:Arizona_State/result">results </a></li>
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  <li><a href="https://2014.igem.org/Team:Arizona_State/notebook">notebook </a></li>
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  <li><a href="https://2014.igem.org/Team:Arizona_State/safety">safety </a></li>
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<a href="https://2014.igem.org/Team:Arizona_State"style="color:#000000">Home </a> </td>
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<a href="https://2014.igem.org/Team:Arizona_State/Team"style="color:#000000"> Team </a> </td>
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<a href="https://igem.org/Team.cgi?year=2014&team_name=Arizona_State"style="color:#000000"> Official Team Profile </a></td>
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<a href="https://2014.igem.org/Team:Arizona_State/Project"style="color:#000000"> Project</a></td>
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<a href="https://2014.igem.org/Team:Arizona_State/Parts"style="color:#000000"> Parts</a></td>
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<a href="https://2014.igem.org/Team:Arizona_State/Modeling"style="color:#000000"> Modeling</a></td>
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<a href="https://2014.igem.org/Team:Arizona_State/Attributions"style="color:#000000"> Attributions </a></td>
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<p> Please be sure to keep these links, your audience will want to find your: </p>
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<li><a href="https://2014.igem.org/Team:Arizona_State">Home</a> </li>
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<li><a href="https://igem.org/Team.cgi?year=2013&team_name=Arizona_State">Official Team Profile</a> </li>
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<p>There are a few wiki requirements teams must follow:</p>
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<p>The Arizona State University iGEM team has taken on a project of creating biodiesel in a new and efficient way using Escherichia coli. Using a wax esterase, fatty acids and ethanol, which are both naturally produced by E. coli, can be combined to form biodiesel. However, E. coli uses the same intermediate products to produce both fatty acids and ethanol, which reduces the efficiency of this process. This problem can be avoided by engineering two different strains of E. coli to work in tandem. One strain will be focused on producing free fatty acids (FFA) through increased production of enzymes such as TesA and acc. The other strain will be focused on producing ethanol through the increased production of enzymes such as pdc and adhB. These two products can be combined in one of the two cells using a waxy esterase, producing the final biodiesel product. This form of cooperative production will hopefully produce more ethanol than current single strain methods using E. coli.</p>
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<li>All pages, images and files must be hosted on the <a href ="https://2014.igem.org/Special:Upload">  2014.igem.org server</a>. </li>
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<p>Visit the <a href="https://2014.igem.org/Wiki_How-To"> Wiki How To page </a> for a complete list of requirements, tips and other useful information. </p>
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            <a class="twitter-timeline" data-dnt="true" href="https://twitter.com/asuigem" data-widget-id="522461432630112256">Tweets by @asuigem</a>
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<p>We are currently working on providing teams with some easy to use design templates.
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<br> In the meantime you can also view other team wikis for inspiration! Here are some very good examples</p>
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<li> <a href="https://2013.igem.org/Team:Colombia_Uniandes">2013 Colombia Unianades </a></li>
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<p>For a full wiki list, you can visit <a href="https://igem.org/Team_Wikis?year=2013">iGEM 2013 web sites </a> and <a href="https://igem.org/Team_Wikis?year=2012">iGEM 2012 web sites</a>  lists. </p>
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<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
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<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="">iGEM 2013 calendar</a> </li>
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Latest revision as of 00:23, 18 October 2014

 

The Arizona State University iGEM team has taken on a project of creating biodiesel in a new and efficient way using Escherichia coli. Using a wax esterase, fatty acids and ethanol, which are both naturally produced by E. coli, can be combined to form biodiesel. However, E. coli uses the same intermediate products to produce both fatty acids and ethanol, which reduces the efficiency of this process. This problem can be avoided by engineering two different strains of E. coli to work in tandem. One strain will be focused on producing free fatty acids (FFA) through increased production of enzymes such as TesA and acc. The other strain will be focused on producing ethanol through the increased production of enzymes such as pdc and adhB. These two products can be combined in one of the two cells using a waxy esterase, producing the final biodiesel product. This form of cooperative production will hopefully produce more ethanol than current single strain methods using E. coli.

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