Team:ETH Zurich/labblog/20140611meet

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== Week 3  ==  
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== Week 3: Plasmid design started, Modeling started ==  
==== Wednesday, June 11th ====
==== Wednesday, June 11th ====
* We started plasmid design :
* We started plasmid design :
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[[File:ETH Zurich Plasmids.png|600px]]
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[[File:ETH Zurich Plasmids.png|center|600px|thumb|First draft of plasmids|First draft of plasmids. The sensor plasmid is the same in every cell. Different logic + repressors plasmids are present in cells p and q. The logic + repressors plasmid from cells p is producing the quorum sensing molecule p, and another version is present in cells q which produces QSq. Colonies of p and q cells will be arranged in an alternate way on the millifluidic chip. Here as an example we use the lux system for p cells and the rhl system for q cells.]]
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&Phi;C31 and Bxb1 are integrases. LuxR an RhlR are quorum sensing molecules. In fact, we don't know yet which quorum sensing systems we will use. We will have to perform cross-talk experimetns in order to choose the ones that are the most orthogonal.
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&Phi;C31 and Bxb1 are integrases. LuxR an RhlR are quorum sensing repressors. A riboswitch construct is placed around quorum sensing constructs to prevent leakiness of lux and rhl promoters. Type p colonies produce AHL by expressing the enzyme LuxI. Type q colonies produce Rhl by expressing the enzyme RhlI. In fact, we don't know yet which quorum sensing systems we will use. We will have to perform cross-talk experimetns in order to choose the ones that are the most orthogonal.
* We tried to print our first agar millifluidic chip : we printed it too small, and the printer had resolution problems.
* We tried to print our first agar millifluidic chip : we printed it too small, and the printer had resolution problems.
* We wrote all reactions and found parameters from the literature for our model.
* We wrote all reactions and found parameters from the literature for our model.
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Latest revision as of 22:00, 11 October 2014