Team:Gifu/Project

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<h1 >WELCOME TO iGEM 2014! </h1>
 
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<p>Your team has been approved and you are ready to start the iGEM season!
 
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<br>On this page you can document your project, introduce your team members, document your progress <br> and share your iGEM experience with the rest of the world! </p>
 
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<br>
 
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<p style="color:#E7E7E7"> <a href="https://2014.igem.org/wiki/index.php?title=Team:Gifu/Project&action=edit"style="color:#FFFFFF"> Click here  to edit this page!</a> </p>
 
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<a href="https://2014.igem.org/Team:Gifu"style="color:#000000">Home </a> </td>
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<a href="https://2014.igem.org/Team:Gifu/Team"style="color:#000000"> Team </a> </td>
 
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<br><br><br><br><br><br><br><br>
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<a href="https://igem.org/Team.cgi?year=2014&team_name=Gifu"style="color:#000000"> Official Team Profile </a></td>
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<h3 style="font-family:Comic Sans MS;font-size:x-large"> Project Description </h3>
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<p class="abc">Tell us more about your project.  Give us background.  Use this as the abstract of your project.  Be descriptive but concise (1-2 paragraphs) </p>
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<td style="border:1px solid black" align="center"  height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7> 
 
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<a href="https://2014.igem.org/Team:Gifu/Project"style="color:#000000"> Project</a></td>
 
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<td style="border:1px solid black;" align="center"  height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
 
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<a href="https://2014.igem.org/Team:Gifu/Parts"style="color:#000000"> Parts</a></td>
 
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<a href="https://2014.igem.org/Team:Gifu/Modeling"style="color:#000000"> Modeling</a></td>
 
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<h3 style="font-family:Comic Sans MS;font-size:x-large">References </h3>
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<a href="https://2014.igem.org/Team:Gifu/Notebook"style="color:#000000"> Notebook</a></td>
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<p class="abc">
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iGEM teams are encouraged to record references you use during the course of your research. They should be posted somewhere on your wiki so that judges and other visitors can see how you though about your project and what works inspired you. </p>  
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<a href="https://2014.igem.org/Team:Gifu/Safety"style=" color:#000000"> Safety </a></td>
 
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<a href="https://2014.igem.org/Team:Gifu/Attributions"style="color:#000000"> Attributions </a></td>
 
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<td align ="center"> <a href="https://2014.igem.org/Main_Page"> <img src="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png" width="55px"></a> </td>
 
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<p>Tell us more about your project.  Give us background.  Use this as the abstract of your project.  Be descriptive but concise (1-2 paragraphs) </p>
 
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<br>
 
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<h3>References </h3>
 
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<p>
 
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iGEM teams are encouraged to record references you use during the course of your research. They should be posted somewhere on your wiki so that judges and other visitors can see how you though about your project and what works inspired you. </p>
 
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<h3 style="font-family:Comic Sans MS;font-size:x-large"> Content</h3>
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<p class="abc"> You can use these subtopics to further explain your project</p>
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<p> You can use these subtopics to further explain your project</p>
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<ol class="abc">
<li><a href="#summary">Overall project summary</a></li>
<li><a href="#summary">Overall project summary</a></li>
<li><a href="#details">Project Details</a></li>
<li><a href="#details">Project Details</a></li>
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It's important for teams to describe all the creativity that goes into an iGEM project, along with all the great ideas your team will come up with over the course of your work.  
It's important for teams to describe all the creativity that goes into an iGEM project, along with all the great ideas your team will come up with over the course of your work.  
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It's also important to clearly describe your achievements so that judges will know what you tried to do and where you succeeded. Please write your project page such that what you achieved is easy to distinguish from what you attempted.  
It's also important to clearly describe your achievements so that judges will know what you tried to do and where you succeeded. Please write your project page such that what you achieved is easy to distinguish from what you attempted.  
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<h1 style="font-family:Comic Sans MS;font-size:x-large"><a name="summary"><font color="black">Overall project summary</font></a></h1>
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<h1><a name="summary"><font color="black">Overall project summary</font></a></h1>
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<h1><a name="experiments"><font color="black">The Experiments</font></a></h1>
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<h2 style="font-family:Comic Sans MS;font-size:x-large">Theme of our project</h2>
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     <p>Transform new year's card to rice cake
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     <p class="abc">Transform new year's card to rice cake
     (make cellulose resolve and reconstruct it to synthesize amylopectin)
     (make cellulose resolve and reconstruct it to synthesize amylopectin)
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     <p>RNA shaped like a ring / ring-shaped RNA
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     <p class="abc">RNA shaped like a ring / ring-shaped RNA
     (mass-produce protein by ring-shaped RNA)
     (mass-produce protein by ring-shaped RNA)
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     <p class="abc">In vivo, proteins are synthesized in transcription and translation.  
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     <p>Today paper is used in quantity every day and will be waste after use.
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    In Japan, for example, they send new year's card to relations, friends and acquaintances every year.
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    So some cards are thrown away when it is written incorrectly, stained or sent from disagreeable person.
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    It is a sheer waste to throw away many cards, so it is great idea that we transform card into MOCHI, tranditional Japanese food made from rice and composed of amylopectin.
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    Paper contains cellulose, and both amylopectin and cellulose are polyglucose.
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    Why we can't the transformation?
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    We genetically modify Escherichia coli and make 2 type of function. One of the function is synthesis of cellulase, which is enzyme analyzes cellulose into glucose. Another is synthesis of amylopectin synthetic enzyme. We use them to analyze cellulose (it compose new year’s card) and synthesize amylopectin (it is main ingredient of MOCHI). In 2008, the iGEM team of the University of Edinburgh studied reducing cellulose to glucose. We refer to this study and improve it to reduce cellulose. On the other hand we introduce gene coding amylopectin synthetic enzyme from Oryza sativa into E.coli to synthesize amylopectin in body of E.coli. In fact, E.coli have a function to synthesize glycogen from glucose in natural. So we use some parts of this function and remove some of them to realize the efficient transformation.
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     <p>In vivo, proteins are synthesized in transcription and translation.  
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     Generally, mRNA is single-strand RNA, starting translation by binding ribosome on initiation codon, and ending by separating ribosome from mRNA.  
     Generally, mRNA is single-strand RNA, starting translation by binding ribosome on initiation codon, and ending by separating ribosome from mRNA.  
     In this study, we aim to build the method of synthesizing long-chain, massive proteins and to improve translation efficiency.  
     In this study, we aim to build the method of synthesizing long-chain, massive proteins and to improve translation efficiency.  
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Latest revision as of 08:11, 29 August 2014


factory1

head home team project note result more










Project Description

Tell us more about your project. Give us background. Use this as the abstract of your project. Be descriptive but concise (1-2 paragraphs)

References

iGEM teams are encouraged to record references you use during the course of your research. They should be posted somewhere on your wiki so that judges and other visitors can see how you though about your project and what works inspired you.

Content

You can use these subtopics to further explain your project

  1. Overall project summary
  2. Project Details
  3. Progress
  4. Materials and Methods
  5. The Experiments
  6. Results
  7. Data analysis
  8. Conclusions

It's important for teams to describe all the creativity that goes into an iGEM project, along with all the great ideas your team will come up with over the course of your work.

It's also important to clearly describe your achievements so that judges will know what you tried to do and where you succeeded. Please write your project page such that what you achieved is easy to distinguish from what you attempted.

Overall project summary

Project Details

Progress

Materials and Methods

The Experiments

Results

Data analysis

Conclusions

Theme of our project

  1. Transform new year's card to rice cake (make cellulose resolve and reconstruct it to synthesize amylopectin)

  2. RNA shaped like a ring / ring-shaped RNA (mass-produce protein by ring-shaped RNA)

Description

  1. In vivo, proteins are synthesized in transcription and translation. Generally, mRNA is single-strand RNA, starting translation by binding ribosome on initiation codon, and ending by separating ribosome from mRNA. In this study, we aim to build the method of synthesizing long-chain, massive proteins and to improve translation efficiency. That is, allowing ribosomes to have a semi-permanent translation mechanism by producing circular mRNA and causing a defect of termination codon. To cyclize mRNA, we can use a splicing mechanism of T4 phage. Splicing is a mechanism removing circular introns which don't code for proteins and joining in exons which code for ones. It occurs after transcription. Splicing is catalyzed by several base sequences of the ends of introns as a ribozyme, being subjected to nucleophilic attack from introns to exons. So we will introduce the plasmids which place the sequence of the end of introns as a splicing ribozyme on the end of a gene coding for proteins into E.coli, and cyclize mRNA for synthesis of long-chain, massive proteins.

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