Team:Sumbawagen

From 2014.igem.org

(Difference between revisions)
 
(77 intermediate revisions not shown)
Line 1: Line 1:
-
<html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en" lang="en" dir="ltr">
+
<html xlns="http://www.w3.org/1999/xhtml" xml:lang="en" lang="en" dir="ltr">
<head>
<head>
<meta http-equiv="Content-Type" content="text/html; charset=utf-8" />
<meta http-equiv="Content-Type" content="text/html; charset=utf-8" />
Line 10: Line 10:
<style type="text/css" media="screen, projection">/*<![CDATA[*/
<style type="text/css" media="screen, projection">/*<![CDATA[*/
@import "/wiki/skins/common/shared.css?270";
@import "/wiki/skins/common/shared.css?270";
-
@import "/wiki/skins/igem/main.css?270";
+
@import "/wiki/skins/igem/main.cs70";
/*]]>*/</style>
/*]]>*/</style>
<link rel="stylesheet" type="text/css" media="print" href="/wiki/skins/common/commonPrint.css?270" />
<link rel="stylesheet" type="text/css" media="print" href="/wiki/skins/common/commonPrint.css?270" />
Line 76: Line 76:
</head>
</head>
-
<body              class="mediawiki  ltr ns-0 ns-subject page-Team_Sumbawagen_Team">
+
<!-- end p-logo -->
-
  <div id="globalWrapper">
+
-
 
+
-
    <div id="top-section">
+
-
<div id="p-logo">
+
-
    <a href="/Main_Page"
+
-
      title="Main Page">
+
-
    <img src='#'>"
+
-
    </a>
+
-
</div>  <!-- end p-logo -->
+
<script type="text/javascript"> if (window.isMSIE55) fixalpha(); </script>
<script type="text/javascript"> if (window.isMSIE55) fixalpha(); </script>
Line 135: Line 126:
       swfobject.registerObject("myFlashContent", "9.0.0", "expressInstall.swf");
       swfobject.registerObject("myFlashContent", "9.0.0", "expressInstall.swf");
     </script>
     </script>
-
     <title>iGEM Dundee 2013 &middot; ToxiMop</title>
+
     <title>iGEM Sumbawagen 2014 &middot; Econey</title>
     <meta name="viewport" content="width=device-width, initial-scale=1.0">
     <meta name="viewport" content="width=device-width, initial-scale=1.0">
-
     <meta name="description" content="A synthetic biology project called Toximop from the Dundee iGEM Team.">
+
     <meta name="description" content="A synthetic biology project called Econey from the Sumbawagen iGEM Team.">
     <meta name="author" content="Sumbawagen Team 2014">
     <meta name="author" content="Sumbawagen Team 2014">
Line 185: Line 176:
               <ul class="nav">
               <ul class="nav">
                 <li class="active"><a href="https://2014.igem.org/Team:Sumbawagen">Home</a></li>
                 <li class="active"><a href="https://2014.igem.org/Team:Sumbawagen">Home</a></li>
-
 
+
         
-
 
+
                 <li class="dropdown">
                 <li class="dropdown">
                   <a href="#" class="dropdown-toggle" data-toggle="dropdown">Overviews <b class="caret"></b></a>
                   <a href="#" class="dropdown-toggle" data-toggle="dropdown">Overviews <b class="caret"></b></a>
                   <ul class="dropdown-menu">
                   <ul class="dropdown-menu">
-
                      <li><a href="#"> Sub Menu 1 </a></li>
+
                       <li><a href="https://2014.igem.org/Team:Sumbawagen/overviews/Econey_Project"> Econey Project </a></li>
-
                      <!--<li><a href="#">Sub Menu 2 </a></li>-->
+
                     </ul>
-
                       <li><a href="https://2014.igem.org/Team:Sumbawagen/Modeling">Modelling </a></li>
+
-
                      <li><a href="#">Sub Menu 4</a></li>
+
-
                     <li><a href="#">Human Practice </a></li>
+
-
                    <!--<li><a href="#">Sub Menu 5</a></li>-->
+
-
                  </ul>
+
                 </li>
                 </li>
Line 205: Line 190:
                   <a href="https://2014.igem.org/Team:Sumbawagen/Team" class="dropdown-toggle" data-toggle="dropdown">Project <b class="caret"></b></a>
                   <a href="https://2014.igem.org/Team:Sumbawagen/Team" class="dropdown-toggle" data-toggle="dropdown">Project <b class="caret"></b></a>
                   <ul class="dropdown-menu">
                   <ul class="dropdown-menu">
-
                    <li class="nav-header">Lab</li>
+
                 
 +
<li><a href="https://2014.igem.org/Team:Sumbawagen/project/econey">Econey</a></li>
 +
<li><a href="https://2014.igem.org/Team:Sumbawagen/project/result">Result</a></li>
 +
<li><a href="https://2014.igem.org/Team:Sumbawagen/project/software">Software</a></li>
 +
<li><a href="https://2014.igem.org/Team:Sumbawagen/parts">Parts</a></li>
 +
<li><a href="https://2014.igem.org/Team:Sumbawagen/Safety">Safety</a></li>
 +
<li><a href="https://2014.igem.org/Team:Sumbawagen/Attributions">Attribution</a></li>
 +
<li><a href="https://2014.igem.org/Team:Sumbawagen/future_direction">Future Direction</a></li>                             
 +
                             
 +
                 
 +
                   
 +
                  </ul>
-
                  <!-- <li><a href="#">Mop</a></li>-->
 
-
<li><a href="#">Sub Menu 11</a></li>
 
-
<li><a href="#">Sub Menu 12</a></li>
 
-
<li><a href="#">Sub Menu 13</a></li>
 
-
                    <li><a href="#">Sub Menu 14 </a></li>
 
-
                    <li><a href="#">Sub Menu <i>Miring</i> </a></li>
 
-
                    <li class="divider"></li>
 
-
                    <li class="nav-header">Modelling </li>
 
-
 
-
                    <!--<li><a href="#">Sub Menu 21</a></li>-->
 
-
                    <li><a href="#">Sub Menu 22</a></li>
 
-
                    <li><a href="#">Sub Menu 23</a></li>
 
-
                    <li><a href="#">Sub Menu 24 </a></li>
 
-
                    <li><a href="#">Sub Menu 25</a></li>
 
-
 
-
                    <li class="divider"></li>
 
-
                    <li class="nav-header">Software &amp; Hardware</li>
 
-
                  <li><a href="#">Glucose Measurement</a></li>
 
-
                  <li><a href="#">Android Application</a></li>
 
-
                  <li><a href="#">Sub Menu 31 </a></li>
 
-
                  </ul>
 
                 </li>
                 </li>
                 <li class="dropdown">
                 <li class="dropdown">
-
                   <a href="#" class="dropdown-toggle" data-toggle="dropdown">Parts <b class="caret"></b></a>
+
                   <a href="#" class="dropdown-toggle" data-toggle="dropdown">Notebook <b class="caret"></b></a>
                   <ul class="dropdown-menu">
                   <ul class="dropdown-menu">
-
                     <li><a href="#">Our Biobricks</a></li>
+
 
 +
                    <li class="nav-header">Daily Notes</li>
 +
                    <li><a href="https://2014.igem.org/Team:Sumbawagen/Notebook">Policy & Practices</a></li>
 +
                    <li><a href="https://2014.igem.org/Team:Sumbawagen/Notebook2">Lab</a></li>
 +
                     <li><a href="https://2014.igem.org/Team:Sumbawagen/Notebook/Protocol">Protocol</a></li>
                     <!--<li><a href="#">Improvements</a></li>-->
                     <!--<li><a href="#">Improvements</a></li>-->
                   </ul>
                   </ul>
                 </li>
                 </li>
-
                 <li><a href="https://2014.igem.org/Team:Sumbawagen/Safety">Safety</a></li>
+
                  
  <li class="dropdown">
  <li class="dropdown">
Line 244: Line 223:
                   <ul class="dropdown-menu">
                   <ul class="dropdown-menu">
                     <li><a href="https://2014.igem.org/Team:Sumbawagen/Team">Meet the Team</a></li>
                     <li><a href="https://2014.igem.org/Team:Sumbawagen/Team">Meet the Team</a></li>
-
                    <li><a href="https://igem.org/Team.cgi?year=2014">Team Information</a></li>
 
                     <li><a href="https://2014.igem.org/Team:Sumbawagen/Team/Gallery">Gallery</a></li>
                     <li><a href="https://2014.igem.org/Team:Sumbawagen/Team/Gallery">Gallery</a></li>
-
                    <li><a href="https://2014.igem.org/Team:Sumbawagen/Notebook">Notebook</a></li>
 
                     <li><a href="https://2014.igem.org/Team:Sumbawagen/Team/Contact">Contact</a></li>
                     <li><a href="https://2014.igem.org/Team:Sumbawagen/Team/Contact">Contact</a></li>
                     <li class="divider"></li>
                     <li class="divider"></li>
                     <li class="nav-header">Social Media</li>
                     <li class="nav-header">Social Media</li>
-
                     <li><a href="https://www.facebook.com/DundeeiGem2013">Facebook</a></li>
+
                     <li><a href="https://www.facebook.com/sumbawagen2014?fref=ts">Facebook</a></li>
-
                     <li><a href="#">Twitter</a></li>
+
                     <li><a href="http://sumbawagen.blogspot.com/">Blog</a></li>
-
                    <li><a href="#">Google+</a></li>
+
-
                    <li><a href="#">Blog</a></li>
+
                   </ul>
                   </ul>
                 </li>
                 </li>
                 <li class="dropdown">
                 <li class="dropdown">
-
                   <a href="#" class="dropdown-toggle" data-toggle="dropdown">Human Practice <!--<sup>*new</sup>--><b class="caret"></b></a>
+
                   <a href="#" class="dropdown-toggle" data-toggle="dropdown">Policy & Practices<!--<sup>*new</sup>--><b class="caret"></b></a>
                   <ul class="dropdown-menu">
                   <ul class="dropdown-menu">
-
                     <li><a href="# ">
+
                   
-
Sub Menu 41
+
                     <li><a href="https://2014.igem.org/Team:Sumbawagen/Human_practice/Policy">
 +
<strong>Policy & Practices</strong>
<div style="   
<div style="   
     font-size: 100%;
     font-size: 100%;
Line 275: Line 251:
</a></li>
</a></li>
-
 
+
<li class="divider"></li>  
-
                    <li><a href="# ">
+
     
-
Sub Menu 42
+
-
<div style="   
+
-
    font-size: 75%;
+
-
    font-size: 100%;
+
-
    font-weight: bold;
+
-
    line-height: 1;
+
-
    color: rgb(66, 139, 202);
+
-
    text-align: center;
+
-
    white-space: nowrap;
+
-
    vertical-align: baseline;
+
-
    display: inline;">
+
-
  <sup>*New</sup></div>
+
</a></li>
</a></li>
 +
<li><a href="https://2014.igem.org/Team:Sumbawagen/Human_practice/survey">Survey</a></li>
 +
<li><a href="https://2014.igem.org/Team:Sumbawagen/Human_practice/Biotech">Biotech Camp</a></li>
 +
<li><a href="https://2014.igem.org/Team:Sumbawagen/Human_practice/meet_the">Meet the farmers</a></li>
 +
<li><a href="https://2014.igem.org/Team:Sumbawagen/Human_practice/meet">Meet US Ambassador</a></li>
 +
<li><a href="https://2014.igem.org/Team:Sumbawagen/Human_practice/econey">@america</a></li>
 +
<li><a href="https://2014.igem.org/Team:Sumbawagen/Human_practice/moyo">Moyo Festival</a></li>
 +
<li><a href="https://2014.igem.org/Team:Sumbawagen/Human_practice/virtual">Virtual Outreach</a></li>
 +
<li><a href="https://2014.igem.org/Team:Sumbawagen/Human_practice/Support">Responses and supports</a></li>
-
<li><a href="#">Media Coverage</a></li>
+
                                                         
-
                    <li><a href="#">Political Campaign</a></li>
+
-
<li><a href="#">Media Coverage</a></li>
+
-
                    <li><a href="#">Clatto Case Study</a></li>
+
-
                    <li><a href="#">Public Engagement</a></li>
+
-
                    <!--<li><a href="#">Interviews</a></li>-->
+
-
                    <li><a href="#">Collaboration</a></li>
+
-
 
+
-
                    <li class="divider"></li>
+
-
                    <li class="nav-header">Media</li>
+
-
                    <li><a href="http://www.youtube.com/channel/UCvHOQ9Y1PqKInj6iCwLqTJw/feed?view_as=public">Youtube Channel</a></li>
+
-
                    <li><a href="http://www.flickr.com/photos/97927329@N05/">Flickr</a></li>
+
-
                    <li><a href="https://2014.igem.org/Team:Sumbawagen/Human Practice/comic">Comic</a></li>
+
-
                    <li><a href="#">Video Game</a></li>
+
-
<li><a href="#">Digital Poster</a></li>
+
-
 
+
-
                   
+
                   </ul>
                   </ul>
                 </li>
                 </li>
Line 314: Line 270:
                  
                  
                 <li class="dropdown">
                 <li class="dropdown">
-
                   <a href="#" class="dropdown-toggle" data-toggle="dropdown">Attributions <b class="caret"></b></a>
+
                   <a href="#" class="dropdown-toggle" data-toggle="dropdown">Interlab Study <b class="caret"></b></a>
                   <ul class="dropdown-menu">
                   <ul class="dropdown-menu">
-
                     <li><a href="/Team:Dundee/Team/Attributions">Attributions</a></li>
+
                     <li><a href="https://2014.igem.org/Team:Sumbawagen/interlabstudy/results">Results</a></li>
-
                    <li><a href="/Team:Dundee/Sponsors">Our Sponsors</a></li>
+
                  </ul>
-
                  </ul>
+
                 </li>
                 </li>
Line 344: Line 299:
               <div class="carousel-inner">
               <div class="carousel-inner">
                 <div class="item active"><!-- add the active class to any slider you want shown first -->
                 <div class="item active"><!-- add the active class to any slider you want shown first -->
-
                   <img src="https://static.igem.org/mediawiki/2014/f/fc/Banner_SBG1.jpg" style="width:100%;height:100%">
+
                   <img src="https://static.igem.org/mediawiki/2014/7/7e/Sumbawa_baner.png" style="width:100%;height:100%">
                 </div>
                 </div>
-
 
+
                  
-
                 <div class="item"><!-- add the active class to any slider you want shown first -->
+
-
                  <img src="https://static.igem.org/mediawiki/2014/3/3b/Banner_SBG2.jpg" style="width:100%;height:100%">
+
-
                </div>
+
-
 
+
-
 
+
                 <div class="item"><!-- add the active class to any slider you want shown first -->
                 <div class="item"><!-- add the active class to any slider you want shown first -->
                   <img src="https://static.igem.org/mediawiki/2014/a/af/Banner_SBG3.jpg" style="width:100%;height:100%">
                   <img src="https://static.igem.org/mediawiki/2014/a/af/Banner_SBG3.jpg" style="width:100%;height:100%">
Line 371: Line 321:
         <p>
         <p>
-
         Sumbawa honey is one of the best honeys in Indonesia. It has become trade mark of Sumbawa and also the main source of income for people of Sumbawa. According to National Standard of Indonesian, honey quality determined based on the quantity of glucose level in the honey. The honey control quality on farmer level was done based on the physical appearance which is not suitable with the industrial standard. Laboratory based on chemical analysis giving the best result measurement but either time or money consuming, it also gives difficulties for the farmer to use. Our team goal was to accommodate the necessity of glucose monitoring level of the Sumbawa honey by create a simple method and device which could be easily applied in the farmer level. <br><br>
+
         BBa_J04450 has a lac promoter and mRFP gene, thus E. coli harboring this plasmid will give red color when the bacteria were grown with lactose or IPTG. Oppositely, the expression of mRFP will be decreased when glucose was present in medium through a mechanism called catabolite repression. We use this phenomenon to measure the concentration of glucose in honey by calibrating the color of E. coli medium using android-based mobile phone. Creation of novel circuit which consists of constitutive promoter followed by either Adenylate Cyclase or IIAGlc genes, which placed downward lac-mRFP circuit, may affect the sensitivity of catabolite repression. We expect an engineered E. coli, which shows red color expression in different range of glucose concentration. By changing mRFP gene with amilCP, a blue fluorescent protein, we may obtain E. coli expressing either red or blue color when different honey added to the medium due to different glucose concentration.<br><br></p>
-
        ECONEY is a genetic engineering of E.coli plasmid with capability to detect different level of glucose concentration in Sumbawa honey by emitting light as the expression of RFP protein, when it placed in the environment contains wide range of glucose. The color expression measurement then transform into smart mobile phone application in order to ease people in measuring glucose level of honey. The color expression of RFP in several dilution of Sumbawa honey used as the standard. In the future ECONEY are expected to become the device that can measure the authenticity of Sumbawa honey.
 
-
        </p>
 
         <br>
         <br>
Line 380: Line 328:
         <div class="span6" style="padding-top:40px" >
         <div class="span6" style="padding-top:40px" >
-
               <iframe src="http://player.vimeo.com/video/69609812?color=c9ff23" width="460" height="281" frameborder="0" webkitAllowFullScreen mozallowfullscreen allowFullScreen></iframe>
+
               <iframe width="460" height="315" src="//www.youtube.com/embed/XICTHe_YXS8" frameborder="0" allowfullscreen></iframe>
           </div>
           </div>
       </div><!-- Row End -->
       </div><!-- Row End -->
Line 403: Line 351:
                 <img id="image-6" src="https://static.igem.org/mediawiki/2014/b/b8/Home_SBG.jpg" style="width:220px;height:220px;"/>
                 <img id="image-6" src="https://static.igem.org/mediawiki/2014/b/b8/Home_SBG.jpg" style="width:220px;height:220px;"/>
               <span class="caption scale-caption" style="text-align:justify">
               <span class="caption scale-caption" style="text-align:justify">
-
                           <p><b style="font-size:16px">Home</b><br><br> Home is our face in our wiki, Sumbawagen Team 2014.</p>
+
                           <p><b style="font-size:16px">Home</b><br><br> </p>
                 </span>
                 </span>
               </div>
               </div>
Line 414: Line 362:
                   <!-- Image Caption 6 -->
                   <!-- Image Caption 6 -->
             <div id="box-6" class="box">
             <div id="box-6" class="box">
-
               <img id="image-6" src="https://static.igem.org/mediawiki/2014/d/d7/Humanpractice_SBG.jpg" style="width:220px;height:220px;"/>
+
               <img id="image-6" src="https://static.igem.org/mediawiki/2014/b/b0/Humanpractice_SBG.png" style="width:220px;height:220px;"/>
               <span class="caption scale-caption" style="text-align:justify">
               <span class="caption scale-caption" style="text-align:justify">
-
                           <p><b style="font-size:16px;">Human Practice</b><br><br> Sumbawa Honey is our pride as Sumbawa Society and with <i>E. coli</i> we will know, How big impact from our project.</p>
+
                           <p><b style="font-size:16px;">Policy & Practices </b><br><br> </p>
               </span>
               </span>
             </div>
             </div>
Line 429: Line 377:
               <img id="image-6" src="https://static.igem.org/mediawiki/2014/f/fa/Team_SBG.jpg" style="width:220px;height:220px;"/>
               <img id="image-6" src="https://static.igem.org/mediawiki/2014/f/fa/Team_SBG.jpg" style="width:220px;height:220px;"/>
               <span class="caption scale-caption" style="text-align:justify">
               <span class="caption scale-caption" style="text-align:justify">
-
                           <p><b style="font-size:16px;">Team</b><br><br>  Understanding and work together, we are unity to shine around the world </p>
+
                           <p><b style="font-size:16px;">Team</b><br><br>  </p>
               </span>
               </span>
             </div>
             </div>
Line 443: Line 391:
               <span class="caption scale-caption" style="text-align:justify">
               <span class="caption scale-caption" style="text-align:justify">
-
                           <p><b style="font-size:16px;">ECONEY Project </b><br><br>This project will be give impact as the function to be an equipment as The Quality Control (QC) and Measurement of Sumbawa honey </p>
+
                           <p><b style="font-size:16px;">ECONEY Project </b><br><br></p>
-
              </span>
+
 
 +
</span>
             </div>
             </div>
           </div></a>
           </div></a>
Line 450: Line 399:
       </div>
       </div>
 +
<!-- Title -->
 +
      <div class="page-header">
 +
          <center><h2><b><strong>Acknowledgment<strong></b> </h2></center>
 +
<center><img src="https://static.igem.org/mediawiki/2014/7/75/BLKM.png"style="width:145px;height:225px;"/><br></center>
 +
 +
        </div>
 +
      <!-- Title End -->
 +
 +
<!-- Title -->
 +
      <div class="page-header">
 +
          <center><h2><b><strong>Sponsors</strong></b> </h2></center>
 +
<center><img src="https://static.igem.org/mediawiki/2014/8/8b/All_sponsor.png"style="width:900px;height:260px;"/><br></center>
       </div><!-- End Page Content -->
       </div><!-- End Page Content -->
Line 526: Line 487:
</div>
</div>
<!-- Served in 0.167 secs. --></body>
<!-- Served in 0.167 secs. --></body>
 +
 +
 +
</p>
 +
<!--
 +
NewPP limit report
 +
Preprocessor node count: 4/1000000
 +
Post-expand include size: 0/2097152 bytes
 +
Template argument size: 0/2097152 bytes
 +
Expensive parser function count: 0/100
 +
-->
 +
 +
<!-- Saved in parser cache with key 2014_igem_org:pcache:idhash:1032-0!1!0!!en!2 and timestamp 20141016110802 -->
 +
<div class="printfooter">
 +
Retrieved from "<a href="https://2014.igem.org/Team:Sumbawagen">https://2014.igem.org/Team:Sumbawagen</a>"</div>
 +
<div id="catlinks"><div id='catlinks' class='catlinks catlinks-allhidden'></div></div> <!-- end content -->
 +
<div class="visualClear"></div>
 +
</div>
 +
    </div>
 +
<!-- PAGE FOOTER -- ITEMS FROM COLUMN ! HAVE BEEN MOVED HERE  -- RDR  -->
 +
<div class="visualClear"></div>
 +
    <div id='footer-box' class='noprint'>
 +
        <div id="footer">
 +
              <div id="f-poweredbyico"><a href="http://www.mediawiki.org/"><img src="/wiki/skins/common/images/poweredby_mediawiki_88x31.png" height="31" width="88" alt="Powered by MediaWiki" /></a></div>       <div id="f-copyrightico"><a href="http://creativecommons.org/licenses/by/3.0/"><img src="http://i.creativecommons.org/l/by/3.0/88x31.png" alt="Attribution 3.0 Unported" width="88" height="31" /></a></div>     <ul id="f-list">
 +
 +
 +
  <!-- Recentchanges is not handles well DEBUG -->
 +
    <li id="t-recentchanges"><a href="/Special:RecentChanges"
 +
      title='Recent changes'>Recent changes</a></li>
 +
 +
    <li id="t-whatlinkshere"><a href="/Special:WhatLinksHere/Team:Sumbawagen"
 +
      title="List of all wiki pages that link here [j]" accesskey="j">What links here</a></li>
 +
 +
                        <li id="t-recentchangeslinked"><a href="/Special:RecentChangesLinked/Team:Sumbawagen"
 +
                          title="Recent changes in pages linked from this page [k]" accesskey="k">Related changes</a></li>
 +
 +
 +
 +
                <li id="t-upload"><a href="/Special:Upload"
 +
                  title="Upload files [u]" accesskey="u">Upload file</a>
 +
                </li>
 +
                <li id="t-specialpages"><a href="/Special:SpecialPages"
 +
                  title="List of all special pages [q]" accesskey="q">Special pages</a>
 +
                </li>
 +
                <li><a href='/Special:Preferences'>My preferences</a></li>
 +
            </ul>
 +
        </div> <!-- close footer -->
 +
        <div id='footer'>
 +
    <ul id="f-list">
 +
 +
            <li id="t-print"><a href="/wiki/index.php?title=Team:Sumbawagen&amp;printable=yes"
 +
              title="Printable version of this page [p]" accesskey="p">Printable version</a>
 +
            </li>
 +
 +
            <li id="t-permalink"><a href="/wiki/index.php?title=Team:Sumbawagen&amp;oldid=269751"
 +
              title="Permanent link to this revision of the page">Permanent link</a>
 +
            </li>
 +
 +
 +
        <li id="privacy"><a href="/2014.igem.org:Privacy_policy" title="2014.igem.org:Privacy policy">Privacy policy</a></li>
 +
        <li id="disclaimer"><a href="/2014.igem.org:General_disclaimer" title="2014.igem.org:General disclaimer">Disclaimers</a></li>
 +
    </ul>
 +
</div> <!-- close footer -->
 +
    </div> <!-- close footer-box -->
 +
 +
<script>if (window.runOnloadHook) runOnloadHook();</script>
 +
</div>
 +
<!-- Served in 0.086 secs. --></body>
</html>
</html>

Latest revision as of 07:50, 19 February 2015

Team:Dundee/Team - 2013.igem.org

 

Team:Sumbawagen/Team

From 2014.igem.org

iGEM Sumbawagen 2014 · Econey

Sumbawagen

Project Description

BBa_J04450 has a lac promoter and mRFP gene, thus E. coli harboring this plasmid will give red color when the bacteria were grown with lactose or IPTG. Oppositely, the expression of mRFP will be decreased when glucose was present in medium through a mechanism called catabolite repression. We use this phenomenon to measure the concentration of glucose in honey by calibrating the color of E. coli medium using android-based mobile phone. Creation of novel circuit which consists of constitutive promoter followed by either Adenylate Cyclase or IIAGlc genes, which placed downward lac-mRFP circuit, may affect the sensitivity of catabolite repression. We expect an engineered E. coli, which shows red color expression in different range of glucose concentration. By changing mRFP gene with amilCP, a blue fluorescent protein, we may obtain E. coli expressing either red or blue color when different honey added to the medium due to different glucose concentration.


Acknowledgment


Sponsors



Retrieved from "https://2013.igem.org/Team:Dundee"

Retrieved from "https://2014.igem.org/Team:Sumbawagen"

Retrieved from "http://2014.igem.org/Team:Sumbawagen"