Team:Sumbawagen

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<h1 style="color:#E7E7E7">Sumbawagen iGEM 2014</h1>
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    <title>iGEM Sumbawagen 2014 &middot; Econey</title>
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                      <li><a href="https://2014.igem.org/Team:Sumbawagen/overviews/Econey_Project"> Econey Project </a></li>
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<li><a href="https://2014.igem.org/Team:Sumbawagen/project/econey">Econey</a></li>
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<li><a href="https://2014.igem.org/Team:Sumbawagen/project/result">Result</a></li>
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<li><a href="https://2014.igem.org/Team:Sumbawagen/project/software">Software</a></li>
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<li><a href="https://2014.igem.org/Team:Sumbawagen/parts">Parts</a></li>
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<li><a href="https://2014.igem.org/Team:Sumbawagen/Safety">Safety</a></li>  
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<li><a href="https://2014.igem.org/Team:Sumbawagen/Attributions">Attribution</a></li>
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<li><a href="https://2014.igem.org/Team:Sumbawagen/future_direction">Future Direction</a></li>                             
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                    <li class="nav-header">Daily Notes</li>
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                    <li><a href="https://2014.igem.org/Team:Sumbawagen/Notebook">Policy & Practices</a></li>
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                    <li><a href="https://2014.igem.org/Team:Sumbawagen/Notebook2">Lab</a></li>
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                    <li><a href="https://2014.igem.org/Team:Sumbawagen/Team">Meet the Team</a></li>
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<strong>Policy & Practices</strong>
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<li><a href="https://2014.igem.org/Team:Sumbawagen/Human_practice/survey">Survey</a></li>
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<li><a href="https://2014.igem.org/Team:Sumbawagen/Human_practice/Biotech">Biotech Camp</a></li>
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<li><a href="https://2014.igem.org/Team:Sumbawagen/Human_practice/meet_the">Meet the farmers</a></li>
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<li><a href="https://2014.igem.org/Team:Sumbawagen/Human_practice/meet">Meet US Ambassador</a></li>
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<li><a href="https://2014.igem.org/Team:Sumbawagen/Human_practice/econey">@america</a></li>
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<li><a href="https://2014.igem.org/Team:Sumbawagen/Human_practice/moyo">Moyo Festival</a></li>
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<li><a href="https://2014.igem.org/Team:Sumbawagen/Human_practice/Support">Responses and supports</a></li>
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<p> Sumbawa honey is one of the best honeys in Indonesia </p>
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        BBa_J04450 has a lac promoter and mRFP gene, thus E. coli harboring this plasmid will give red color when the bacteria were grown with lactose or IPTG. Oppositely, the expression of mRFP will be decreased when glucose was present in medium through a mechanism called catabolite repression. We use this phenomenon to measure the concentration of glucose in honey by calibrating the color of E. coli medium using android-based mobile phone. Creation of novel circuit which consists of constitutive promoter followed by either Adenylate Cyclase or IIAGlc genes, which placed downward lac-mRFP circuit, may affect the sensitivity of catabolite repression. We expect an engineered E. coli, which shows red color expression in different range of glucose concentration. By changing mRFP gene with amilCP, a blue fluorescent protein, we may obtain E. coli expressing either red or blue color when different honey added to the medium due to different glucose concentration.<br><br></p>
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                        Sumbawa honey is one of the best honeys in Indonesia. It has become trade mark of Sumbawa and also the main source of income for people of Sumbawa. According to National Standard of Indonesian, honey quality determined based on the quantity of glucose level in the honey. The honey control quality on farmer level was done based on the physical appearance which is not suitable with the industrial standard. Laboratory based on chemical analysis giving the best result measurement but either time or money consuming, it also gives difficulties for the farmer to use.
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Our team goal was to accommodate the necessity of glucose monitoring level of the Sumbawa honey by create a simple method and device which could be easily applied in the farmer level.
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<b> ECONEY </b> is a genetic engineering of E.coli plasmid with capability to detect different level of glucose concentration in Sumbawa honey by emitting light as the expression of RFP protein, when it placed in the environment contains wide range of glucose. The color expression measurement then transform into smart mobile phone application in order to ease people in measuring glucose level of honey. The color expression of RFP in several dilution of Sumbawa honey used as the standard. In the future <b> ECONEY </b> are expected to become the device that can measure the authenticity of Sumbawa honey.
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Latest revision as of 07:50, 19 February 2015

Team:Dundee/Team - 2013.igem.org

 

Team:Sumbawagen/Team

From 2014.igem.org

iGEM Sumbawagen 2014 · Econey

Sumbawagen

Project Description

BBa_J04450 has a lac promoter and mRFP gene, thus E. coli harboring this plasmid will give red color when the bacteria were grown with lactose or IPTG. Oppositely, the expression of mRFP will be decreased when glucose was present in medium through a mechanism called catabolite repression. We use this phenomenon to measure the concentration of glucose in honey by calibrating the color of E. coli medium using android-based mobile phone. Creation of novel circuit which consists of constitutive promoter followed by either Adenylate Cyclase or IIAGlc genes, which placed downward lac-mRFP circuit, may affect the sensitivity of catabolite repression. We expect an engineered E. coli, which shows red color expression in different range of glucose concentration. By changing mRFP gene with amilCP, a blue fluorescent protein, we may obtain E. coli expressing either red or blue color when different honey added to the medium due to different glucose concentration.


Acknowledgment


Sponsors



Retrieved from "https://2013.igem.org/Team:Dundee"

Retrieved from "https://2014.igem.org/Team:Sumbawagen"

Retrieved from "http://2014.igem.org/Team:Sumbawagen"