Team:Bielefeld-CeBiTec/Project/CO2-fixation

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<h1> CO<sub>2</sub> Fixation </h1>
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<h1> CO2 fixation </h1>
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  <h6>Short summary</h6>
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    <p>In the second module we aim to use a carboxysome which is found in cyanobacteria or purple sulfurbacteria. With this compartment we want to create a Calvin-Benson cycle in <i>E. coli</i>. In addition we would like to compare the efficiency of the carboxysome with a free RuBisCO (Ribulose-1,5-bisphosphate-carboxylase-oxygenase), the 3-Hydroxypropionate cycle or other types of carboxysomes. The product of the fixation will be pyruvate which can be used for production of different metabolites like for example <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Project/Isobutanol">Isobutanol</a>.
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<a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Results/CO2-fixation">Here</a> you will find the results of the CO<sub>2</sub> fixation.</p>
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    <p>The second module we aim to use a carboxysome of cyanobacteria or purple sulfurbacteria. With this we want to create a Calvin-Benson cycle in <i>E.coli</i>. In addition we would like to compare the efficiency of the carboxysome with a free RuBisCO (Ribulose-1,5-bisphosphate-carboxylase-oxygenase), the 3-Hydroxypropionate cycle or other types of carboxysomes. The product of the fixation will be pyruvate which can be used for production of different metabolites like for example <a href=https://2014.igem.org/Team:Bielefeld-CeBiTec/Project/Isobutanol">Isobutanol</a>.</p>
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<h4 class="member" style="margin-left:20px">References</h4>
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        Andersson, 2008. Catalysis and regulation in Rubisco. <a href="http://jxb.oxfordjournals.org/content/59/7/1555.full.pdf">Journal of Experimental Botany</a>, vol. 59, pp. 1555-1568
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        Berg (2011) Ecological Aspects of the Distribution of Different Autotrophic CO<sub>2</sub> Fixation Pathways <a href="http://aem.asm.org/content/77/6/1925">Applied and Environmental Microbiology</a>, vol. 77, no. 6, pp. 1925-1936
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        Bonacci et al., 2011. Modularity of carbon-fixing protein organelle. <a href="http://www.pnas.org/content/109/2/478" target="_blank">PNAS</a>, vol. 109, pp. 478-483
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      <li id="field1998">
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        Field et al., 1998. Primary Production of Biosphere: Integrating Terrestrial and Oceanic Components. <a href="http://www.sciencemag.org/content/281/5374/237.full" target="_blank">Science</a>, vol. 281, pp. 237-240
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      Mann, 1999. Genetic Engineers Aim to Soup up Crop Photosynthesis. <a href="http://www.sciencemag.org/content/283/5400/314.full" target="_blank">Science</a>, vol. 283, pp. 314-316
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      Parikh et al., 2006. Directed evolution of RuBisCO hypermorphs through genetic selection in engineered <i>E.coli</i>. <a href="http://peds.oxfordjournals.org/content/19/3/113.long" target="_blank">Protein Engineering, Design & Selection</a>, vol. 19, pp. 113-119
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      Evans et al., 1966. A new ferredoxin dependent carbon reduction cycle in a photosynthetic bacterium. <a href="http://www.jstor.org/stable/57493" target="_blank">Proc. Natl. Acad. Sci. U. S. A.</a>, vol. 55, pp.928-934
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      Rosenthal et al., 2011. Overexpressing the C(3) photosynthesis cycle enzyme sedoheptulose 1,7-bisphosphatase improves photosynthetic carbon gain and yield under fully open air CO(2) fumigation (FACE).<a href="http://www.biomedcentral.com/1471-2229/11/123" target="_blank">BMC Plant Biol.</a>, vol. 11, pp. 123
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      Stolzenberger et al., 2013. Characterization of Fructose 1,6-Bisphosphatase and Sedoheptulose 1,7-Bisphosphate from the Facultative Ribulose Monophosphate Cycle Methylotroph <i>Bacillus methanolicus</i>. <a href="http://jb.asm.org/content/195/22/5112.long" target="_blank">Journal of Bacteriology</a>, Vol. 195, pp. 5112-5122
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Latest revision as of 09:24, 15 October 2014


CO2 Fixation

Short summary

In the second module we aim to use a carboxysome which is found in cyanobacteria or purple sulfurbacteria. With this compartment we want to create a Calvin-Benson cycle in E. coli. In addition we would like to compare the efficiency of the carboxysome with a free RuBisCO (Ribulose-1,5-bisphosphate-carboxylase-oxygenase), the 3-Hydroxypropionate cycle or other types of carboxysomes. The product of the fixation will be pyruvate which can be used for production of different metabolites like for example Isobutanol.

Here you will find the results of the CO2 fixation.

References

  • Andersson, 2008. Catalysis and regulation in Rubisco. Journal of Experimental Botany, vol. 59, pp. 1555-1568
  • Berg (2011) Ecological Aspects of the Distribution of Different Autotrophic CO2 Fixation Pathways Applied and Environmental Microbiology, vol. 77, no. 6, pp. 1925-1936
  • Bonacci et al., 2011. Modularity of carbon-fixing protein organelle. PNAS, vol. 109, pp. 478-483
  • Field et al., 1998. Primary Production of Biosphere: Integrating Terrestrial and Oceanic Components. Science, vol. 281, pp. 237-240
  • Mann, 1999. Genetic Engineers Aim to Soup up Crop Photosynthesis. Science, vol. 283, pp. 314-316
  • Parikh et al., 2006. Directed evolution of RuBisCO hypermorphs through genetic selection in engineered E.coli. Protein Engineering, Design & Selection, vol. 19, pp. 113-119
  • Evans et al., 1966. A new ferredoxin dependent carbon reduction cycle in a photosynthetic bacterium. Proc. Natl. Acad. Sci. U. S. A., vol. 55, pp.928-934
  • Rosenthal et al., 2011. Overexpressing the C(3) photosynthesis cycle enzyme sedoheptulose 1,7-bisphosphatase improves photosynthetic carbon gain and yield under fully open air CO(2) fumigation (FACE).BMC Plant Biol., vol. 11, pp. 123
  • Stolzenberger et al., 2013. Characterization of Fructose 1,6-Bisphosphatase and Sedoheptulose 1,7-Bisphosphate from the Facultative Ribulose Monophosphate Cycle Methylotroph Bacillus methanolicus. Journal of Bacteriology, Vol. 195, pp. 5112-5122

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