Team:ITB Indonesia/Project

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<p>Your team has been approved and you are ready to start the iGEM season!
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<br>On this page you can document your project, introduce your team members, document your progress <br> and share your iGEM experience with the rest of the world! </p>
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<p style="color:#E7E7E7"> <a href="https://2014.igem.org/wiki/index.php?title=Team:ITB_Indonesia/Project&action=edit"style="color:#FFFFFF"> Click here  to edit this page!</a> </p>
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<a href="https://2014.igem.org/Team:ITB_Indonesia/Team"style="color:#000000"> Team </a> </td>
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<a href="https://igem.org/Team.cgi?year=2014&team_name=ITB_Indonesia"style="color:#000000"> Official Team Profile </a></td>
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<a href="https://2014.igem.org/Team:ITB_Indonesia/Project"style="color:#000000"> Project</a></td>
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<p> PET or commonly known as plastic has been widely used ranging from domestic to industry. We can’t deny that plastic gives us benefits. Despite a lot of benefits that we can take, the overproduction of plastic has serious impact on the environment if not treated properly. Increasing 7% global plastic production annually has been estimated to likely exceed 24.39 million tons in 2015. Appropriate treatment is needed, otherwise plastic abundant will be treathening surroundings, the life of animal and human beings. As we know that PET will take 500 or even 1000 years to degrade by process called photodegradation rather than biodegradation, thus making a necessity to treat plastic such as degradation or recycling. Degradation by burning or using chemical methode will also bring harmfull effect. Recycling is jut an alternative that won’t overcome this problem completely, while reuse seems inappropriate to do due to plastic age. When it’s reused severe health complication may occur.
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Based on this case, we want to diversify the methode on treating plastic overproduction using synthetic biological approach. We create novel device which is synthetic bacteria that can degrade PET and also consume the product of degradation reaction ( ethylene glycol ). We establish ompA fused with LC Cutinase on E.coli BL21 giving rise to displaying enzymes on the surface of bacteria. Thus making ease on the degradation process which substrate ( in this case PET ) with high molecular weight doen’t need to accross cell membrane. We also construct a casette comprised of two enzymes, Glycoaldehide reductase and glycoaldehide dehidrogenase which responsible to metabolize ethylene glycol. We use constitutive promoter for both  construct. Thus making us necessery to create a module of reporter and self regulatory in order to make system is efficiently to run and minimizing severe cytoplasmic stress, in this case inclusion body aggregate due to strong constitutive expression.
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<h3>References </h3>
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iGEM teams are encouraged to record references you use during the course of your research. They should be posted somewhere on your wiki so that judges and other visitors can see how you though about your project and what works inspired you. </p>
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<p> You can use these subtopics to further explain your project</p>
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<li>Conclusions</li>
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It's important for teams to describe all the creativity that goes into an iGEM project, along with all the great ideas your team will come up with over the course of your work.
 
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia">HOME</a></li>
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<li>TEAM
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<li><a href="https://igem.org/Team.cgi?id=1387" >OFFICIAL TEAM PROFILE</a></li>
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/Team">TEAM MEMBER</a></li>
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<li>PROJECT
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/Project">OVERVIEW</a></li>
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/DegMod">DEGRADATION MODULE</a></li>
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/ConMod">CONVERTION MODULE</a></li>
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/RepMod">REPORTER MODULE</a></li>
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/SelfMod">SELF REGULATORY MODULE</a></li>
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/FutureSystem">FUTURE SYSTEM</a></li>
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/Modeling">MODELING</a></li>
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<li>WETLAB
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/protocol">PROTOCOL</a></li>
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/Parts">PARTS</a></li>
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/Attributions">ATTRIBUTIONS</a></li>
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/Safety">SAFETY</a></li>
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/Data">DATA</a></li>
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/ProteinModel">PROTEIN MODEL</a></li>
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/Achievement">ACHIEVEMENT</a></li>
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<li>NOTEBOOK
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<li>HUMAN PRACTICE
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/Unpas">INTRO SYNBIO TO UNPAS</a></li>
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/SynBio">SYNBIO CLASS</a></li>
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/SynbiGreen">SYNBIGREEN SURVEY</a></li>
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/Medsos">SOCIAL MEDIA</a></li>
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/OnMedia">MEDIA</a></li>
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/Biosafety">BIOSAFETY SEMINAR</a></li>
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/MeetUp">INDONESIA TEAMS MEET UP</a></li>
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/Kids">FUN WITH KIDS</a></li>
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/UPI">SHARING SYNBIO IN UPI</a></li>
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<h1>Project Overview</h1>
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<p align="justify">Team ITB_Indonesia tries to create a new method to degrade plastic using synthetic biology approach. We create a novel device which is a synthetic bacteria that able to degrade PET and consume ethylene glycol as the byproduct of degradation reaction. We also establish ompA protein fused with LC Cutinase in <i>E.coli</i> BL21(DE3) thus exposing the enzymes on the surface membrane of bacteria. This condition make the degradation process easily because PET, a high molecular substrate, doesn't need to pass through bacteria cell membrane. We also construct a cassette comprised of two enzyme, glycoaldehyde reductase and glycoaldehyde dehydrogenase which responsible in metabolising ethylene glycol. We use constitutive promoter for both construct, thus making it necessary to create a modul of reporter and self regulatory in order to maintain system efficiency and minimizing severe cytoplasmic stress, in this case, formation of inclusion body aggregate due to strong constitutive expression.</p>
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Latest revision as of 03:40, 17 October 2014


Project Overview

Team ITB_Indonesia tries to create a new method to degrade plastic using synthetic biology approach. We create a novel device which is a synthetic bacteria that able to degrade PET and consume ethylene glycol as the byproduct of degradation reaction. We also establish ompA protein fused with LC Cutinase in E.coli BL21(DE3) thus exposing the enzymes on the surface membrane of bacteria. This condition make the degradation process easily because PET, a high molecular substrate, doesn't need to pass through bacteria cell membrane. We also construct a cassette comprised of two enzyme, glycoaldehyde reductase and glycoaldehyde dehydrogenase which responsible in metabolising ethylene glycol. We use constitutive promoter for both construct, thus making it necessary to create a modul of reporter and self regulatory in order to maintain system efficiency and minimizing severe cytoplasmic stress, in this case, formation of inclusion body aggregate due to strong constitutive expression.



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