Team:Macquarie Australia

From 2014.igem.org

(Difference between revisions)
 
(151 intermediate revisions not shown)
Line 3: Line 3:
<head>
<head>
-
<link href="https://2013.igem.org/Team:Macquarie_Australia/style/Home?action=raw&ctype=text/css" rel="stylesheet">
+
<link href="https://2014.igem.org/Template:Team:Macquarie_Australia/css/global?action=raw&ctype=text/css" rel="stylesheet">
-
<script>
+
<script src="https://2014.igem.org/Team:Macquarie_Australia/js/banner?action=raw&ctype=text/javascript" type="text/javascript""></script>
-
$(document).ready(function() {
+
</head>
-
//Display slider for Javascript users
+
<body>
-
$('#Slider').css('display', 'block');
+
<section id="HomePageContent">
-
+
<div id="Slider">
-
//Set height of slider (16/5 ratio)
+
<div id="Slide0" class="Slides current"></div>
-
$('#Slider').css('height', ($('body').width() / 3.2));
+
<div id="Slide1" class="Slides"></div>
-
+
<div id="Slide2" class="Slides"></div>
-
//Position right/left arrows
+
<div id="Slide4" class="Slides"></div>
-
var BarHeight = $('#Slider').height() -  $('#BottomLinks').height();
+
<div id="Slide3" class="Slides"></div>
-
$('#LeftBar, #RightBar').css('height', BarHeight);
+
<div id="LeftBar" class="SideBar"><div id="LeftArrow"></div></div>
-
$('#LeftArrow, #RightArrow').css('top', (BarHeight-60)/2);
+
<div id="RightBar" class="SideBar"><div id="RightArrow"></div></div>
 +
<ul id="BottomLinks">
 +
<li id="Link0" class="SlideLink">Overview</li>
 +
<li id="Link1" class="SlideLink">Idea</li>
 +
<li id="Link2" class="SlideLink">Situation</li>
 +
<li id="Link4" class="SlideLink">Outcome</li>
 +
<li id="Link3" class="SlideLink">Future</li>
 +
</ul>
 +
</div>
-
//Repeats for window resize
+
<section id="Overview">
-
$(window).resize(function() {
+
-
$('#Slider').css('height', ($('body').width() / 3.2));
+
-
+
-
var BarHeight = $('#Slider').height() -  $('#BottomLinks').height();
+
-
$('#LeftBar, #RightBar').css('height', BarHeight);
+
-
$('#LeftArrow, #RightArrow').css('top', (BarHeight-60)/2);
+
-
});
+
-
+
-
var TotalSlides = $('.Slides').length;
+
-
var CurrentSlide = 0;
+
-
$('#RightArrow').click(function() {
+
<h1>Our project</h1>
-
CurrentSlide = ++CurrentSlide % TotalSlides;
+
<h2 style="text-align: center;">Watch this cool video below!</h2>
-
changeSlide(CurrentSlide);
+
-
});
+
-
+
-
$('#LeftArrow').click(function() {
+
-
CurrentSlide = (CurrentSlide + TotalSlides - 1) % TotalSlides;
+
-
changeSlide(CurrentSlide);
+
-
});
+
-
+
-
$('.SlideLink').click(function() {
+
-
var ClickedSlide = $(this).attr('id');
+
-
CurrentSlide = parseInt(ClickedSlide.replace('Link', ''));
+
-
changeSlide(CurrentSlide);
+
-
});
+
-
+
-
function changeSlide(num){
+
-
var SlideID = '#Slide' + num;
+
-
$('.Slides').css('display', 'none');
+
-
$(SlideID).css('display', 'block');
+
-
}
+
-
});
+
-
</script>
+
-
</head>
+
-
<body>
+
-
<section id="HomePageContent">
+
-
<div id="Slider">
+
-
<div id="Slide0" class="Slides current"></div>
+
-
<div id="Slide1" class="Slides"></div>
+
-
<div id="Slide2" class="Slides"></div>
+
-
<div id="Slide3" class="Slides"></div>
+
-
<div id="Slide4" class="Slides"></div>
+
-
<div id="Slide5" class="Slides"></div>
+
-
<div id="LeftBar" class="SideBar"><div id="LeftArrow"></div></div>
+
-
<div id="RightBar" class="SideBar"><div id="RightArrow"></div></div>
+
-
<ul id="BottomLinks">
+
-
<li id="Link0" class="SlideLink">Intro</li><li id="Link1" class="SlideLink">Problem</li><li id="Link2" class="SlideLink">Situation</li><li id="Link3" class="SlideLink">Idea</li><li id="Link4" class="SlideLink">Solution</li><li id="Link5" class="SlideLink">Platform</li>
+
-
</ul>
+
-
</div>
+
 +
<html>
 +
<video class='center' poster="https://static.igem.org/mediawiki/2014/e/e4/Video.png" controls>
 +
<source src="https://static.igem.org/mediawiki/2014/3/3d/Prezi_Movie_3.mp4" type='video/mp4'/>
 +
</video>
 +
</html><html>
 +
<br/><br/>
-
<section id="Overview">
+
<h1></h1>
-
<h2>Our Project</h2>
+
-
<p>
+
-
Text1
+
-
</p>
+
-
<p>Text2</p>
+
<h3>Overview</h3>
-
</section>
+
<p>This project has demonstrated functionality of our designed operons that represent the first step of the chlorophyll a biosynthesis. This was performed through the initial assembly of three operons containing the essential biosynthetic genes that were confirmed through gel electrophoresis and DNA sequencing (Fig. 1).  The functionality of the first operon (Mg-chelatase) was demonstrated through the spectral analysis of its enzymatic product, Mg-Protophoryin IX (which can be seen on the <a href="https://2014.igem.org/Team:Macquarie_Australia/Project/Results">Results</a> page). </p><br/>
-
+
 
-
<section id="SectionLinks">
+
<img id="PathwayImg" src="https://static.igem.org/mediawiki/2014/0/08/Homepic1.png" />
-
<div class="Wrap">
+
<p><b>Figure 1.</b> Flow chart of the chlorophyll a synthesis pathway. Operons containing the essential genes from <i>Chlamydomonas reinhardtii</i> are represented for their respective steps within the pathway. The spectral change of the compounds are represented in their respective colours of each step.</p>
-
<a href="Team:Macquarie_Australia/style/Home">
+
 
-
<div id="DescriptionLink1" class="DescriptionLink">
+
<p>We have also modelled this step of the biosynthetic pathway (which can be seen on the <a href="https://2014.igem.org/Team:Macquarie_Australia/Project/Model">Modelling</a> page). The project was successful in building the foundations for future teams to complete the synthesis of photosystem II in <i>E. coli.</i> This provides a significant leap into the development of a hydrogen-generating bacterial system and a renewable biological energy source. Our policy and practice initiatives were also successful for increasing public awareness of the global energy crisis and the potential synthetic biology has to offer in  providing a solution (which can be seen on the <a href="https://2014.igem.org/Team:Macquarie_Australia/Outreach">Policy & Practice</a> page). </p>
-
<h2>Lorem ipsum</h2>
+
</section>
-
<img src="https://static.igem.org/mediawiki/2013/a/ab/Mqbutton55.png">
+
       
-
<p> Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua.</p>
+
<section id="SectionLinks">
-
</div>
+
<h2 style="text-align: center;">A huge thank you to our Gold sponsors!</h2>
-
</a>
+
<img src="https://static.igem.org/mediawiki/2014/3/3d/Goldsponsors.png" />
-
+
<h2 style="text-align: center;">Also many thanks to our Bronze sponsors!</h2>
-
<a href="Team:Macquarie_Australia/style/Home">
+
<img src="https://static.igem.org/mediawiki/2014/e/ea/Bronzelogo.png" />
-
<div id="DescriptionLink2" class="DescriptionLink">
+
-
<h2>Lorem ipsum</h2>
+
-
<img src="https://static.igem.org/mediawiki/2013/a/ab/Mqbutton55.png">
+
-
<p> Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua.</p>
+
-
</div>
+
-
</a>
+
-
<div style="clear:both;"></div>
+
-
<a href="Team:Macquarie_Australia/style/Home">
+
-
<div id="DescriptionLink3" class="DescriptionLink">
+
-
<h2>Lorem ipsum</h2>
+
-
<img src="https://static.igem.org/mediawiki/2013/a/ab/Mqbutton55.png">
+
-
<p> Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua.</p>
+
-
</div>
+
-
</a>
+
-
+
-
<a href="Team:Macquarie_Australia/style/Home">
+
-
<div id="DescriptionLink4" class="DescriptionLink">
+
-
<h2>Lorem ipsum</h2>
+
-
<img src="https://static.igem.org/mediawiki/2013/a/ab/Mqbutton55.png">
+
-
<p> Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua.</p>
+
-
</div>
+
-
</a>
+
-
</div>
+
-
</section>
+
-
+
</section>
</section>
-
</body>
+
</section>
-
</html>
+
</body>
 +
</html></html>

Latest revision as of 03:43, 18 October 2014

Our project

Watch this cool video below!



Overview

This project has demonstrated functionality of our designed operons that represent the first step of the chlorophyll a biosynthesis. This was performed through the initial assembly of three operons containing the essential biosynthetic genes that were confirmed through gel electrophoresis and DNA sequencing (Fig. 1). The functionality of the first operon (Mg-chelatase) was demonstrated through the spectral analysis of its enzymatic product, Mg-Protophoryin IX (which can be seen on the Results page).


Figure 1. Flow chart of the chlorophyll a synthesis pathway. Operons containing the essential genes from Chlamydomonas reinhardtii are represented for their respective steps within the pathway. The spectral change of the compounds are represented in their respective colours of each step.

We have also modelled this step of the biosynthetic pathway (which can be seen on the Modelling page). The project was successful in building the foundations for future teams to complete the synthesis of photosystem II in E. coli. This provides a significant leap into the development of a hydrogen-generating bacterial system and a renewable biological energy source. Our policy and practice initiatives were also successful for increasing public awareness of the global energy crisis and the potential synthetic biology has to offer in providing a solution (which can be seen on the Policy & Practice page).

</html>

Retrieved from "http://2014.igem.org/Team:Macquarie_Australia"