Team:UT-Dallas/Notebook/8-05

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List out mis-match of gRNA&reporter vectors.
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Inoculate reporter vector from glycerol stock.
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Test digest for reporter vectors August 5
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<a href="https://2014.igem.org/Team:UT-Dallas"style="color:#ffffff; text-decoration: none; text-transform: uppercase; font-weight: bold;" >Home </a> </td>
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<a href="https://igem.org/Team.cgi?year=2014&team_name=UT-Dallas"style="color:#ffffff; text-decoration: none; text-transform: uppercase; font-weight: bold;"> Official Profile </a></td>
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<a href="https://2014.igem.org/Team:UT-Dallas/Modeling"style="color:#ffffff; text-decoration: none; text-transform: uppercase; font-weight: bold;"> Modeling</a></td>
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<a href="https://2014.igem.org/Team:UT-Dallas/Safety"style=" color:#ffffff; text-decoration: none; text-transform: uppercase; font-weight: bold;"> Safety </a></td>
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<a href="https://2014.igem.org/Team:UT-Dallas/Attributions"style="color:#ffffff; text-decoration: none; text-transform: uppercase; font-weight: bold;"> Attributions </a></td>
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<a href="https://2014.igem.org/Team:UT-Dallas/Human-Practices"style="color:#ffffff; text-decoration: none; text-transform: uppercase; font-weight: bold;"> Human Practices</a></td>
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<h1 class="firstHeading" align="center">Tuesday, August 05, 2014</h1>
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<center><p><a href="https://2014.igem.org/wiki/index.php?title=Team:UT-Dallas/Notebook/8-05&action=edit "style="color:#87A96B" >Click here to edit the page </a> </p></center>
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  We started using Kristina's home stock cells now instead of our own cells along with the commercial cells.</p>
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  We have a set of reporter vectors that we grew and harvested the DNA last week. Today, to qualitatively check that we got what we wanted, we are going to test digest our reporter vectors. After that is checked, we will send our second batch of sequencing order.</p>
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<p class="tab">Kristina brought lots of food: cookies, muffins, etc.
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      <th><font size="3">Today's tasks:</font>
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<LI> Transform.
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<LI> List out mis-match of gRNA&reporter vectors.
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        <LI> Inoculate reporter vector from glycerol stock.
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      <br> Preparing cells for plasmid purification.
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Latest revision as of 04:30, 17 October 2014

Home Team Official Profile Project Parts Modeling Notebook Safety Attributions Human Practices

Tuesday, August 05, 2014

Click here to edit the page

We started using Kristina's home stock cells now instead of our own cells along with the commercial cells.

We have a set of reporter vectors that we grew and harvested the DNA last week. Today, to qualitatively check that we got what we wanted, we are going to test digest our reporter vectors. After that is checked, we will send our second batch of sequencing order.

Kristina brought lots of food: cookies, muffins, etc.

Today's tasks:
  • Transform.
  • List out mis-match of gRNA&reporter vectors.
  • Inoculate reporter vector from glycerol stock.


Preparing cells for plasmid purification.

Retrieved from "http://2014.igem.org/Team:UT-Dallas/Notebook/8-05"