Team:UT-Dallas/Notebook/8-04

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<h1 class="firstHeading" align="center">Monday, August 4th, 2014</h1>
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<h1 class="firstHeading" align="center">Monday, August 4th, 2014 </h1>
<center><p><a href="https://2014.igem.org/wiki/index.php?title=Team:UT-Dallas/Notebook/8-04&action=edit "style="color:#87A96B" >Click here to edit the page </a> </p></center>
<center><p><a href="https://2014.igem.org/wiki/index.php?title=Team:UT-Dallas/Notebook/8-04&action=edit "style="color:#87A96B" >Click here to edit the page </a> </p></center>
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   <p> We assembling the plasmid for the chromoproteins for bio-arts and potential outreach program later. We also assemble 7 of Kortne's reporter vectors that we didn't do before(Promoter+target-sequence+RBS+eYFP), 5 reporter vectors that we are sending for sequencing.</p>
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   <p> Today we did a gel purification of our overnight digested promoter, inoculated any color colonies from chromoproteins,transformed ligation's for reporter vectors, and prepared gRNA vectors for sequencing   </p>
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  <p> Tentative plan for tomorrow </P>
    
    
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       <th><font size="3">Today's tasks:</font>
       <th><font size="3">Today's tasks:</font>
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<p>Transformed overnight Ligation.</p>
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<p>Digest more promoters.</p>
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<p>Gel & gel purify reporter vectors.</p>
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<LI> Digest more promoter vectors (we ran out).
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<LI> List item one
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<LI> Test digest gRNA and reporter vectors for ctxB.
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<LI> List item two
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        <LI> Overnight ligation of chromoprotein with promoter, reporter vectors with promoter.
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  <LI> List item three
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  <LI> List item four
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  <LI> List item five
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      <img src="https://static.igem.org/mediawiki/2014/2/25/August_4_2.jpg" height="250px">
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      <br> Colonies!!!!
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Latest revision as of 04:25, 17 October 2014

Home Team Official Profile Project Parts Modeling Notebook Safety Attributions Human Practices

Monday, August 4th, 2014

Click here to edit the page

Today we did a gel purification of our overnight digested promoter, inoculated any color colonies from chromoproteins,transformed ligation's for reporter vectors, and prepared gRNA vectors for sequencing

Tentative plan for tomorrow

Today's tasks:

Transformed overnight Ligation.

Digest more promoters.

Gel & gel purify reporter vectors.
  • List item one
  • List item two
  • List item three
  • List item four
      <--------If you need subcategories--------->
    • Subcat 1
    • Subcat 2
    • Subcat 3
    • Subcat 4
  • List item five


Colonies!!!!

Retrieved from "http://2014.igem.org/Team:UT-Dallas/Notebook/8-04"