Team:Warwick/Project

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<a href="https://2014.igem.org/Team:Warwick/Project"style="color:#000000"> <b>  </b> </a> <a href=https://2014.igem.org/Team:Warwick/Modeling><img src="https://static.igem.org/mediawiki/2014/3/3c/Modeling.png" border="20" align=20px" height ="20px" alt="Image and video hosting by TinyPic">
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<a href="https://2014.igem.org/Team:Warwick/Project"style="color:#000000"> <b>  </b> </a> <a href=https://2014.igem.org/Team:Warwick/Notebook><img src="https://static.igem.org/mediawiki/2014/9/95/Notebook.png
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<a href=https://2014.igem.org/Team:Warwick/Attributions><img src="https://static.igem.org/mediawiki/2014/archive/1/16/20140806085423%21Sponsors.png
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            <li> <a href = "/Team:Warwick"> HOME </a> </li>
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            <li> <a href = "/Team:Warwick/Project"> <span> PROJECT </span> </a> </li>
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            <li> <a href = "/Team:Warwick/Team"> TEAM </a> </li>
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            <li> <a href = "/Team:Warwick/Parts"> PARTS </a> </li>
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            <li> <a href = "/Team:Warwick/Modelling"> MODELLING </a> </li>
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            <li> <a href = "/Team:Warwick/Notebook"> NOTEBOOK </a> </li>
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            <li> <a href = "/Team:Warwick/Human"> POLICY & PRACTICES </a> </li>
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            <!--<li> <a href = "/Team:Warwick/Measurements"> MEASUREMENTS </a> </li>-->
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            <li> <a href = "/Team:Warwick/Interlab"> INTERLAB </a> </li>
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<li> <a href = "/Team:Warwick/Project/Motivation"> MOTIVATION </a> </li>
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<li> <a href = "/Team:Warwick/Project/Howitworks"> HOW IT WORKS </a> </li>
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<li> <a href = "/Team:Warwick/Project/Applications"> APPLICATIONS </a> </li>
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            <!-- THIS IS WHERE YOUR MAIN BODY GOES -->
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<h2>WELCOME TO RNA WORLD</h2>
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<p> Welcome to the biological world which we have spent an entire summer creating and perfecting. We are introducing RNA to synthetic biology in a big way by directly contributing a number of key parts to iGEM.  We have taken a risk, we have been told our project is unrealistic and should be scrapped, that a group of undergraduates are crazy for even thinking about doing something so complicated. At times, we doubted ourselves- there have been good times and bad times.  Spurred on by a desire to make a difference to synthetic biology and to the world in which we live, we pressed forward - a new team to iGEM, not seasoned veterans. We take chances and risks to make a difference - whether success comes our way or failure, we embrace the moment, cherishing it. We hope that the synthetic biology community will appreciate our efforts, as RNA usage and popularity surges. DNA best serves as a template for information storage whereas RNA is multi-functional, catalytic and dynamic. Welcome to our world - the RNA world!<br>
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Our modelling in this project has several aims :
 
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<b>- Warwick iGEM Team 2014 </b>
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<li>To find the amount of DPP-IV reduction reached when the system reaches equilibrium</li>
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<li>To find a way to control the level of DPP-IV reduction</li>
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<li>To find the minimum number of RdRps, replicons, etc to be initially transfected into the cell, which are required to achieve a steady state for the system</li>
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<li>To find out how long does it take for the system to reach equilibrium</li>
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<li>To find out the level of reduction we need to treat diabetes</li>
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<li>To find out how stable the system is (i.e. will the system only work in very specific situations, or in lots of different systems?)
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We are currently using Simbiology in Matlab and Copasi to model the system. We are currently adapting several different models, which come from research into HCV replicons, to our system. If our models can be made to fit our experiments well, we may extend our project to try and find a way to control the level of DPP-IV which is reduced. In addition modelling the system will allow it to be better optimised in the future, and optimum values for constants such as the strength of the ribosome binding sites, and the number of siRNAs produced by each degradation, so that the effect of our biobrick can be optimised.<p><p>
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Initially we determined that our system should reach some equilibrium after a certain amount of time. This is because firstly, HCV is a successful virus, so the replicons should not completely degrade away as time goes to infinity.  Secondly, since there are only a finite amount of resources within the cell, the number of replicons in the system cannot keep increasing forever. This means either the number of replicons must tend towards a certain constant (constant with respect to time), or the number of replicons should tend towards oscillations. <p><p>
 
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<p><center><a href="/Team:Warwick/Project/Motivation"><img src="https://static.igem.org/mediawiki/parts/d/d4/RNA_world.PNG" height="50%" width="50%"/></a></center></p>
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  <p>Created by: iGEM Warwick</p>
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  <p>Contact information: <a href="mailto:igem.warwick@gmail.com">
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Latest revision as of 03:40, 18 October 2014

WELCOME TO RNA WORLD

Welcome to the biological world which we have spent an entire summer creating and perfecting. We are introducing RNA to synthetic biology in a big way by directly contributing a number of key parts to iGEM. We have taken a risk, we have been told our project is unrealistic and should be scrapped, that a group of undergraduates are crazy for even thinking about doing something so complicated. At times, we doubted ourselves- there have been good times and bad times. Spurred on by a desire to make a difference to synthetic biology and to the world in which we live, we pressed forward - a new team to iGEM, not seasoned veterans. We take chances and risks to make a difference - whether success comes our way or failure, we embrace the moment, cherishing it. We hope that the synthetic biology community will appreciate our efforts, as RNA usage and popularity surges. DNA best serves as a template for information storage whereas RNA is multi-functional, catalytic and dynamic. Welcome to our world - the RNA world!
- Warwick iGEM Team 2014



Retrieved from "http://2014.igem.org/Team:Warwick/Project"