We constitute our team in November 2013 and we have meeting twice a month until April. Then we met every weeks. The Lab work started at the beginning of June.
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==Notebook==
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We constituted our team in November 2013 and we had meetings twice a month until April. Then we met every week. The Lab work started in the beginning of June.
Use our calendar to have a look on our notebook and our filter to go directly to Lab Work, Reunion, Human Practices or Modeling work.
Use our calendar to have a look on our notebook and our filter to go directly to Lab Work, Reunion, Human Practices or Modeling work.
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[https://2014.igem.org/wiki/index.php?title=Team:Paris_Saclay/Notebook&action=edit Click here to edit this page]
*Culture of the supercompetent cells MG1655 and MG1655Z1 [https://2014.igem.org/Team:Paris_Saclay/Notebook/June/30 30th June]
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*Phages preparation for the transduction [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/2 2nd July]
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*Phage transduction with the P1 phages in the strains MG1655 and MG1655Z1 [3rd July]
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*Cultures of MG1655 and MG1655Z1 [4th July]
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*Transformations test of competent cells MG1655 and MG1655Z1 [10th July]
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*Preparation of competent cells E coli, deletion of the antibiotic cassette in the odorless bacteria [11th July]
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*Preparation of competent cells E. coli MG1655 and MG1655Z1 [17th July]
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*Transformation of supercompetent cells MG1655 and MG1655Z1 [18th July]
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*Results of the transformation [21st July]
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*Preparation and transformation of competent MG1655 and MG1655Z1 [22nd July]
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*PCR verification of the strains grown [25th July]
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*Preparation and Transformation of competent MG1655 and MG1655Z1 with BT340 [28th July]
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*PCR of MG1655 and MG1655Z1 with FTR-Apra-F and FTR-Apra-R [29th July]
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*Preparation and transformation of electrocompetent MG1655 and MG1655Z1 with BT340 [30th July]
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*Stria of MG1655 and MG1655Z1 on LB dishes [1st August]
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*Stria of MG1655 and MG1655Z1 on LB and kan dishes [4th August]
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*PCR verification of the final strains MG1655 and MG1655Z1 [5th August]
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*Final stock of MG1655 [6th August]
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===C - Salicylate Inducible Suppressing System===
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*Rehydration of BioBricks BBa_J61051 and BBa_K228001- [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/23#C_-_The_Suppressing_Salicylate_System 23th July]
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*Transformation of competent E.coli cells - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/24#C_-_Salicylate_Inducible_Suppressing_System 24th July]
*Plasmid DNA Purification - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/6#C_-_Salicylate_Inducible_Suppressing_System 6th August]
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*Digestion of BBa_B0015 and BBa_K1372000 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/6#C_-_Salicylate_Inducible_Suppressing_System 6th August]
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*Segregate Process - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/6#C_-_Salicylate_Inducible_Suppressing_System 6th August]
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*DNA extraction from gel agarose - ''7th August''
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*Ligation - ''7th August''
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*Transformation of competent E.coli cells - ''8th August''
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*Liquid Culture - ''11th August''
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*Plasmid DNA Purification - ''12th August''
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*Digestion to check & Electrophoresis - ''12th August''
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*Final Stock - ''12th August''
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===D - Lemon Scent===
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* PCR of BBa_517003 and BBa_K762100 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/4#Polymerase_chain_reaction 4th August]
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* Gel electrophoresis of BBa_K517003 and BBa_K762100 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/5#Electrophoresis 5th August]
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* PCR Cleanup of BBa_K517003 and BBa_K762100 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/6#PCR_Cleanup 6th August]
We constituted our team in November 2013 and we had meetings twice a month until April. Then we met every week. The Lab work started in the beginning of June.
Use our calendar to have a look on our notebook and our filter to go directly to Lab Work, Reunion, Human Practices or Modeling work.
"
