Team:UT-Dallas/Notebook/8-01

From 2014.igem.org

(Difference between revisions)
 
(7 intermediate revisions not shown)
Line 1: Line 1:
{{CSS/Main}}
{{CSS/Main}}
<html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en" lang="en" dir="ltr">
<html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en" lang="en" dir="ltr">
 +
 +
<!--navigation menu -->
 +
<table align="center">
 +
<td align="center" colspan="12">
 +
 +
<table  width="100%">
 +
<tr height="15px"></tr>
 +
<tr height="75px">
 +
 +
<td style="display: inline; padding: 15px; text-decoration: none; " align="center" height ="45px" onMouseOver="this.bgColor='#4E817B'" onMouseOut="this.bgColor='#70B9B0'" bgColor=#70B9B0> 
 +
<a href="https://2014.igem.org/Team:UT-Dallas"style="color:#ffffff; text-decoration: none; text-transform: uppercase; font-weight: bold;" >Home </a> </td>
 +
 +
<td style="display: inline; padding: 15px; text-decoration: none;" align="center" height ="45px" onMouseOver="this.bgColor='#4E817B'" onMouseOut="this.bgColor='#70B9B0'" bgColor=#70B9B0>
 +
<a href="https://2014.igem.org/Team:UT-Dallas/Team"style="color:#ffffff; text-decoration: none; text-transform: uppercase; font-weight: bold;"> Team </a> </td>
 +
 +
<td style="display: inline; padding: 15px; text-decoration: none;" align="center"  height ="45px"  onMouseOver="this.bgColor='#4E817B'" onMouseOut="this.bgColor='#70B9B0'" bgColor=#70B9B0>
 +
<a href="https://igem.org/Team.cgi?year=2014&team_name=UT-Dallas"style="color:#ffffff; text-decoration: none; text-transform: uppercase; font-weight: bold;"> Official Profile </a></td>
 +
 +
<td style="display: inline; padding: 15px; text-decoration: none;" align="center"  height ="45px" onMouseOver="this.bgColor='#4E817B'" onMouseOut="this.bgColor='#70B9B0'" bgColor=#70B9B0> 
 +
<a href="https://2014.igem.org/Team:UT-Dallas/Project"style="color:#ffffff; text-decoration: none; text-transform: uppercase; font-weight: bold;"> Project</a></td>
 +
 +
<td style="display: inline; padding: 15px; text-decoration: none;" align="center"  height ="45px" onMouseOver="this.bgColor='#4E817B'" onMouseOut="this.bgColor='#70B9B0'" bgColor=#70B9B0>
 +
<a href="https://2014.igem.org/Team:UT-Dallas/Parts"style="color:#ffffff; text-decoration: none; text-transform: uppercase; font-weight: bold;"> Parts</a></td>
 +
 +
<td style="display: inline; padding: 15px; text-decoration: none;" align="center" height ="45px" onMouseOver="this.bgColor='#4E817B'" onMouseOut="this.bgColor='#70B9B0'" bgColor=#70B9B0>
 +
<a href="https://2014.igem.org/Team:UT-Dallas/Modeling"style="color:#ffffff; text-decoration: none; text-transform: uppercase; font-weight: bold;"> Modeling</a></td>
 +
 +
<td style="display: inline; padding: 15px; text-decoration: none;" align="center" height ="45px" onMouseOver="this.bgColor='#4E817B'" onMouseOut="this.bgColor='#70B9B0'" bgColor=#70B9B0> 
 +
<a href="https://2014.igem.org/Team:UT-Dallas/Notebook"style="color:#ffffff; text-decoration: none; text-transform: uppercase; font-weight: bold;"> Notebook</a></td>
 +
 +
<td style="display: inline; padding: 15px; text-decoration: none;" align="center"  height ="45px" onMouseOver="this.bgColor='#4E817B'" onMouseOut="this.bgColor='#70B9B0'" bgColor=#70B9B0>
 +
<a href="https://2014.igem.org/Team:UT-Dallas/Safety"style=" color:#ffffff; text-decoration: none; text-transform: uppercase; font-weight: bold;"> Safety </a></td>
 +
 +
<td style="display: inline; padding: 15px; text-decoration: none;" align="center"  height ="45px" onMouseOver="this.bgColor='#4E817B'" onMouseOut="this.bgColor='#70B9B0'" bgColor=#70B9B0>
 +
<a href="https://2014.igem.org/Team:UT-Dallas/Attributions"style="color:#ffffff; text-decoration: none; text-transform: uppercase; font-weight: bold;"> Attributions </a></td>
 +
 +
<td style="display: inline; padding: 15px; text-decoration: none;" align="center"  height ="45px" onMouseOver="this.bgColor='#4E817B'" onMouseOut="this.bgColor='#70B9B0'" bgColor=#70B9B0>
 +
<a href="https://2014.igem.org/Team:UT-Dallas/Human-Practices"style="color:#ffffff; text-decoration: none; text-transform: uppercase; font-weight: bold;"> Human Practices</a></td>
 +
 +
 +
<td style="display: inline; padding: 5px;" align ="center"> <a href="https://2014.igem.org/Team:UT-Dallas"> <img src="https://static.igem.org/mediawiki/2014/e/e1/UTDmodifiedlogo.jpg" height="45px"></a></td>
 +
<td style="display: inline; padding: 5px;" align ="center"> <a href="https://2014.igem.org/Main_Page"> <img src="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png" width="55px"></a> </td>
 +
 +
</tr>
 +
</table>
 +
</tr>
 +
 +
 +
</tr>
 +
 +
 +
</td>
 +
 +
<tr> <td colspan="3"  height="15px"> </td></tr>
 +
<tr><td bgColor="#e7e7e7" colspan="3" height="1px"> </tr>
 +
<tr> <td colspan="3"  height="5px"> </td></tr>
 +
</table>
 +
 +
<!------end nav-->
<h1 class="firstHeading" align="center">Friday, August 1st, 2014</h1>
<h1 class="firstHeading" align="center">Friday, August 1st, 2014</h1>
Line 19: Line 78:
<!--##################################-->
<!--##################################-->
-
<table align="center" cellspacing="0" cellpadding="0" border="0" width="50%" >
+
<table align="center" cellspacing="5" cellpadding="5" border="0" width="70%" >
   <thead>
   <thead>
     <tr>
     <tr>
Line 32: Line 91:
<LI> Transform ligation from yesterday (Promoter-RBS-ctxB)plus negative and positive controls. Ligation was done at 16 degrees overnight.  
<LI> Transform ligation from yesterday (Promoter-RBS-ctxB)plus negative and positive controls. Ligation was done at 16 degrees overnight.  
<LI> Miniprep tcfF-RBS-eYFP (PCR product)
<LI> Miniprep tcfF-RBS-eYFP (PCR product)
 +
<LI> Run test digestion on aforementioned miniprep.
<LI> Streak chromoprotein stab cultures from registry:
<LI> Streak chromoprotein stab cultures from registry:
<UL>
<UL>
Line 47: Line 107:
       </td>
       </td>
       <td>
       <td>
-
<img class="pic" src="" width="300px" align="right"/>
+
<img class="pic" src="https://static.igem.org/mediawiki/2014/f/f0/UTDallasAugust1i1.jpg" width="300px" align="right"/>
 +
<td> <img class="pic" src="https://static.igem.org/mediawiki/2014/0/08/UTDallasAugust1i2.jpg"width="300px" align="right:/>
       </td>
       </td>
     </tr>
     </tr>
Line 59: Line 120:
   <p>
   <p>
       <img src="" height="270px">
       <img src="" height="270px">
-
       <br> We have iGEM-icecream today!.
+
        
       <div class="gallerylayer" opacity: 1; background: white;">
       <div class="gallerylayer" opacity: 1; background: white;">
       <img src="" style="border-width: 0px; display: inline; margin-left: 0px; margin-top: 0px;" height="200px">
       <img src="" style="border-width: 0px; display: inline; margin-left: 0px; margin-top: 0px;" height="200px">

Latest revision as of 03:12, 17 October 2014

Home Team Official Profile Project Parts Modeling Notebook Safety Attributions Human Practices

Friday, August 1st, 2014

Click here to edit the page


A relatively short day in the lab. We are in the process of fundamentally improving our cloning protocols after failure of 40 of our gRNA plates.

On Saturday, Megan will come in to inoculate colonies from today's transformation of ctxB as well as the new chromoprotein plates. On Sunday, Sam will come to do a miniprep of all these parts.


Today's tasks:

  • Transform ligation from yesterday (Promoter-RBS-ctxB)plus negative and positive controls. Ligation was done at 16 degrees overnight.
  • Miniprep tcfF-RBS-eYFP (PCR product)
  • Run test digestion on aforementioned miniprep.
  • Streak chromoprotein stab cultures from registry:
    • BBa_K1033905: RBS + tsPURPLE, purple chromoprotein
    • BBa_K1033918: RBS + gfasPURPLE, purple chromoprotein
    • BBa_K1033913: RBS + scORANGE, orange chromoprotein
    • BBa_K1033915: RBS + amajLIME, green chromoprotein
    • BBa_k1033925: RBS + spisPINK, pink chromoprotein
    • BBa_K1033927: RBS + asPINK, pink chromoprotein
    • BBa_K1033930: RBS + amilCP, blue/purple chromoprotein