Team:UT-Dallas/Notebook/8-01

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==August 1st, 2014==
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<h1 class="firstHeading" align="center">Friday, August 1st, 2014</h1>
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<center><p><a href="https://2014.igem.org/wiki/index.php?title=Team:UT-Dallas/Notebook/8-01&action=edit "style="color:#87A96B" >Click here to edit the page </a> </p></center>
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  <p> A relatively short day in the lab. We are in the process of fundamentally improving our cloning protocols after failure of 40 of our gRNA plates. </p>
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<p> On Saturday, Megan will come in to inoculate colonies from today's transformation of ctxB as well as the new chromoprotein plates. On Sunday, Sam will come to do a miniprep of all these parts.</P>
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      <th><font size="3">Today's tasks:</font></th>     
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<LI> Transform ligation from yesterday (Promoter-RBS-ctxB)plus negative and positive controls. Ligation was done at 16 degrees overnight.
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<LI> Miniprep tcfF-RBS-eYFP (PCR product)
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<LI> Run test digestion on aforementioned miniprep.
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<LI> Streak chromoprotein stab cultures from registry:
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<LI> BBa_K1033905: RBS + tsPURPLE, purple chromoprotein
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<LI> BBa_K1033918: RBS + gfasPURPLE, purple chromoprotein
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<LI> BBa_K1033913: RBS + scORANGE, orange chromoprotein
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<LI> BBa_K1033915: RBS + amajLIME, green chromoprotein
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<LI> BBa_k1033925: RBS + spisPINK, pink chromoprotein
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<LI> BBa_K1033927: RBS + asPINK, pink chromoprotein
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<LI> BBa_K1033930: RBS + amilCP, blue/purple chromoprotein
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<p> Today's tasks </p>
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Latest revision as of 03:12, 17 October 2014

Home Team Official Profile Project Parts Modeling Notebook Safety Attributions Human Practices

Friday, August 1st, 2014

Click here to edit the page

A relatively short day in the lab. We are in the process of fundamentally improving our cloning protocols after failure of 40 of our gRNA plates.

On Saturday, Megan will come in to inoculate colonies from today's transformation of ctxB as well as the new chromoprotein plates. On Sunday, Sam will come to do a miniprep of all these parts.

Today's tasks:
  • Transform ligation from yesterday (Promoter-RBS-ctxB)plus negative and positive controls. Ligation was done at 16 degrees overnight.
  • Miniprep tcfF-RBS-eYFP (PCR product)
  • Run test digestion on aforementioned miniprep.
  • Streak chromoprotein stab cultures from registry:
    • BBa_K1033905: RBS + tsPURPLE, purple chromoprotein
    • BBa_K1033918: RBS + gfasPURPLE, purple chromoprotein
    • BBa_K1033913: RBS + scORANGE, orange chromoprotein
    • BBa_K1033915: RBS + amajLIME, green chromoprotein
    • BBa_k1033925: RBS + spisPINK, pink chromoprotein
    • BBa_K1033927: RBS + asPINK, pink chromoprotein
    • BBa_K1033930: RBS + amilCP, blue/purple chromoprotein

Retrieved from "http://2014.igem.org/Team:UT-Dallas/Notebook/8-01"