Team:Cambridge-JIC/Constructs/gal4 hap1gr H2RBmRFP

From 2014.igem.org

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<b> Some error in the digestion protocol is likely to have occurred. </b>
<b> Some error in the digestion protocol is likely to have occurred. </b>
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To insert a promoter, a digest with Kpn1 was performed. Gel was illegible, although a faint band at 13kb may have been visible.
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To insert a promoter, a digest with Kpn1 was performed. Gel was illegible, although a faint band at 13kb may have been visible. No extraction was made due to poor quality of the gel.
Attempt 2:
Attempt 2:
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<li>Comments:</li>
<li>Comments:</li>
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</ul> Grew up in LB 8 more colonies. Attempted colony PCR using inoculation loop in dry PCR tube.
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Grew up in LB 8 more colonies. Attempted colony PCR using inoculation loop in dry PCR tube - will wait to see results from GEL. Expect an 8kb fragment: unlikely that this will show results. Will perform a SalI digest with correct protocol on 31/07 following miniprep from colonies.
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<b> Some error in the digestion protocol is likely to have occurred. </b>
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To insert a promoter, a digest with Kpn1 was performed. Gel was illegible.  
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Latest revision as of 16:10, 30 July 2014