Team:DTU-Denmark/Team/Attributions

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<pageheader>Attributions</pageheader>
<pageheader>Attributions</pageheader>
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<h2>Overview</h2>
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<p>Several months of brainstorming, discussions and literature search as well as meetings with different faculty members from the Technical University of Denmark resulted in a joint decision on working on the Spinach project. The initial idea about measuring promoter activities in absolute terms originates from one of our team members, Kristian Jensen, and Chris Workman hinted us about the Spinach concept. The obvious applicability of the project and huge potential has been a main driving force for all of us during the project.</p>
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<p>The formation of our team was facilitated by Chris Workman, but the project management, planning, fundraising as well as the designs, work described on this wiki, our results and the submitted BioBricks were done by ourselves. Nevertheless, we owe thanks to several people for constructive feedback, advice, guidance and for encouraging our work as well as for providing us space in their laboratories.</p>
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<th colspan="2" scope="col"><h2>Overview</h2></th>
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        <th rowspan="2" scope="col">Several months of brainstorming, discussions and literature search as well as meetings with different faculty members from the Technical University of Denmark resulted in a joint decision on working on the Spinach project. The initial idea about measuring promoter strengths in absolute terms originates from one of our team members, Kristian Jensen, and associate professor Chris Workman hinted us about the spinach concept. The obvious applicability of the project and huge potential has been a main driving force for all of us, together with our fascination of XX (something with green or spinach).</th>
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<th scope="col">The formation of our team was facilitated by Associate Professor Chris Workman, but the project management, planning, fundraising as well as the designs, work described on this wiki, our results and our submitted BioBricks was done by ourselves. Nevertheless, we owe a thank to several people for constructive feedback, advice, guidance and for encouraging our work.</th>
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<th colspan="2" scope="col"><h2>Students</h2></th>
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<th colspan="2" scope="col">Initially we divided the team into different subgroups created with regard to our competences at that time and personal intentions for gaining new skills. Our contributions therefore very much reflect which subgroups we have been in.</th>
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<th colspan="2" scope="col"><h2>Instructors and Advisors</h2></th>
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<th colspan="2" scope="col">Our instructors and advisors have provided constructive feedback and advice, when needed. </th>
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        <th rowspan="2" scope="col"><img src="https://static.igem.org/mediawiki/2014/8/8b/DTU-Denmark-Life_Science_and_Beyond.png" width="250" style="float:right" style= "margin-left:10px"></th>
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<th scope="col"><header3>Life Science and Beyond</header3><br>In order to inspire younger university students and spread the word about iGEM we participated in a conference dealing with different initiatives for students interested in expanding their studies to exceed the ordinary study programme. Many students showed  interested in iGEM. Tell about what synthetic biology implies. </th>
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        <th rowspan="2" scope="col"><img src="https://static.igem.org/mediawiki/2014/9/9f/DTU-Denmark-Biobrick1.png" width="250" style="float:right" style= "margin-left:10px"></th>
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<th scope="col"><header3>Biobrick Workshop</header3><br>Knowledge sharing is an essential part of the development of project.
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We arranged a workshop for the other iGEM teams. <br>
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The DTU iGEM team 2014 decided to repeat the success of the BioBrick workshop hosted by the DTU iGEM team of 2013. The BioBrick workshop is a gathering of the iGEM teams of Denmark (KU, SDU and DTU) for a social learning experience about the core of iGEM and genetical engineering.
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        <th rowspan="2" scope="col"><img src="https://static.igem.org/mediawiki/2014/9/96/DTU-Denmark-Biobrick2.png" width="250" style="float:right" style= "margin-left:10px"></th>
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<th scope="col">The BioBrick workshop was hosted over a weekend at DTU where the teams received lectures about; Primer design for USER-cloning, the online tool PHUSER that can be used for designing primers, the core of iGEM, how to receive medals and Wiki design and editing in iGEM. The teams were also introduced to several need to know laboratory procedures; PCR, Gel electrophoresis, Purification of DNA from agarose gels and USER-cloning.<br>
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The overall idea was to give the teams a solid introduction to the iGEM competition, the wiki and a basic hands-on experience with essential laboratory techniques when working with engineering of biological systems.<br>
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<h2>Students</h2>
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<p>Initially we divided the team into different subgroups created with regard to our competences at the time of our competence session and personal intentions for gaining new skills. Due to this subdivision, the contributions of each member is greatly reflected by the subgroups each of us has participated in during the project. The members of the different subgroups are as follows:</p>
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<b>Laboratory</b>
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<tr><td>- Kristian Jensen</td><td>- Rasmus Bech</td><td>- Kristian Barrett</td></tr>
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<tr><td>- Caroline Mosbech</td><td>- Anne Pihl Bali</td><td>- Anne Sofie Lærke Hansen</td></tr>
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<b>Policy & Practice</b>
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<tr><td>- Caroline Mosbech</td><td>- Kristian Jensen</td><td>- Kristian Barrett</td></tr>
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<tr><td>- Anne Pihl Bali</td><td>- Anne Sofie Lærke Hansen</td><td></td><td></td></tr>
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<b>Fundraising</b>
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<tr><td>- Anne Pihl Bali</td><td>- Caroline Mosbech</td><td>- Anne Sofie Lærke Hansen</td></tr>
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<tr><td>- Kristian Jensen</td><td>- Kristian Barrett</td><td></td><td></td></tr>
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<b>Wiki</b>
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<tr><td>- Thor Bech Johannesen</td><td>- Kristian Jensen</td><td>- Rasmus Bech</td></tr>
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<tr><td>- Mark Thomas Østerlund</td><td>- Kristian Barrett</td><td>- Anne Sofie Lærke Hansen</td></tr>
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<tr><td>- Caroline Mosbech</td><td>- Anne Pihl Bali</td><td></td><td></td></tr>
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Another important purpose was to generate team feeling within the different teams, this was achieved by social dinners after days of lectures and exercises in the lab. </th>
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<p>A couple of students who were part of the initial phase of the project should receive a special thanks.</p>
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    <li>Anders Bech Bruntse, was of great help in proofreading funding applications resulting in full financing of our project and trip to Boston.</li>
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    <li>Casper Nørskov Enghuus, took part in the initial fundraising process and also took part in the initial lab phase.</li>
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    <li>Georgi Dimitrov created the spinach iGEM logo.</li>
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</ul>
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</br>
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<h2>Advisors</h2>
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<p>Our advisors have advised us and provided constructive feedback as well as professional discussions, when this was needed and we owe every one of them thanks.</p>
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<p>Especially we owe <i>Kristian Davidsen</i> thanks for his practical help during the project. Kristian has provided both ‘emergency’ lab  help and tips and tricks for PCR. Furthermore Kristian was of great help during our BioBrick workshop.</p>
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<p><i>Hilde Coumou</i> has helped in the initial phase of our Policy & Practice work. She has introduced us to different communication strategies and how to optimise science communication, which has helped us in defining a strategy for streamlining our outreach initiatives as well as choosing our target group.</p>
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<p><i>Helen Cook</i> helped us set up the BioBrick workshop and has kindly answered all kinds of immediate questions throughout the project period. </p>
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<p>In addition to helping us with the scientific aspects of our project and the experimental planning, <i>Jan Martinussen</i> and <i>Mogens Kilstrup</i> have opened up their lab to us so we could use their equipment for measuring fluorescence.</p>
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<p><i>Ali Altintas</i> has helped us throughout our experimental work with the BioLector, as well as with interpreting our results throughout the project. Furthermore Ali gave us the initial safety introduction to the laboratory.</p>
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<p><i>Anne Egholm Pedersen</i> has introduced us to fluorometry and helped with our flow cytometry measurements.</p>
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<h2>Acknowledgements</h2>
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<p>We are very thankful for the help and guidance that we have received from other faculty members than our own supervisors, such as</p>
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<ul>
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    <li>Sébastien Muller, for advice on work with <i>in vitro</i> transcription.</li>
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    <li>Martin Kogle provided us with competent <i>E. coli</i> DH5&alpha;.</li>
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    <li>Regina Schürmann, for helping out with laboratory facilities during the BioBrick workshop.</li>
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    <li>Marzanna Pulka-Amin, for materials in the laboratory</li>
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    <li>Marlene Dalgaard, for advice and materials for <i>in vitro</i> transcription.</li>
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</ul>
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</br>
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<h2>Other iGEM Teams</h2>
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<p>During the summer an ethic workshop was hosted by the <a href="https://2014.igem.org/Team:UNIK_Copenhagen/Workshop" Target="_blank">University of Copenhagen iGEM team</a>, the outcome of the workshop can be seen <a href="https://2014.igem.org/Team:DTU-Denmark/Overview/Ethics">here</a>, and we are very grateful to have been invited.</p>
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</br>
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<h2>Laboratories</h2>
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<p>Most of our experiments were carried out at Center for Biological Sequence Analysis (CBS) at DTU Systems Biology located at the Technical University of Denmark. Here we were invited to use their machines to perform our molecular work and measurements.</p>
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<p>Part of the work was carried out at Metabolic Signaling and Regulation and Infection Microbiology at DTU Systems Biology. Here we used the fluorescence microscope and fluorometer to investigate our cultures, which was a great help during the project and we used the flow cytometer to measure fluorescence at cellular level.</p>
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</br>
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<h2>Literature</h2>
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<p>Dr. Samie Jaffrey from Department of Pharmacology, Weill Medical College, Cornell University, was of great help, and provided us the specific sequences of their Spinach-construct which later on made the foundation for our quantification of promoter strengths.</p>
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Latest revision as of 02:34, 18 October 2014

Technical University of Denmark

Attributions

Overview

Several months of brainstorming, discussions and literature search as well as meetings with different faculty members from the Technical University of Denmark resulted in a joint decision on working on the Spinach project. The initial idea about measuring promoter activities in absolute terms originates from one of our team members, Kristian Jensen, and Chris Workman hinted us about the Spinach concept. The obvious applicability of the project and huge potential has been a main driving force for all of us during the project.

The formation of our team was facilitated by Chris Workman, but the project management, planning, fundraising as well as the designs, work described on this wiki, our results and the submitted BioBricks were done by ourselves. Nevertheless, we owe thanks to several people for constructive feedback, advice, guidance and for encouraging our work as well as for providing us space in their laboratories.

Students

Initially we divided the team into different subgroups created with regard to our competences at the time of our competence session and personal intentions for gaining new skills. Due to this subdivision, the contributions of each member is greatly reflected by the subgroups each of us has participated in during the project. The members of the different subgroups are as follows:


Laboratory
- Kristian Jensen- Rasmus Bech- Kristian Barrett
- Caroline Mosbech- Anne Pihl Bali- Anne Sofie Lærke Hansen

Policy & Practice
- Caroline Mosbech- Kristian Jensen- Kristian Barrett
- Anne Pihl Bali- Anne Sofie Lærke Hansen

Fundraising
- Anne Pihl Bali- Caroline Mosbech- Anne Sofie Lærke Hansen
- Kristian Jensen- Kristian Barrett

Wiki
- Thor Bech Johannesen- Kristian Jensen- Rasmus Bech
- Mark Thomas Østerlund- Kristian Barrett- Anne Sofie Lærke Hansen
- Caroline Mosbech- Anne Pihl Bali

A couple of students who were part of the initial phase of the project should receive a special thanks.

  • Anders Bech Bruntse, was of great help in proofreading funding applications resulting in full financing of our project and trip to Boston.
  • Casper Nørskov Enghuus, took part in the initial fundraising process and also took part in the initial lab phase.
  • Georgi Dimitrov created the spinach iGEM logo.

Advisors

Our advisors have advised us and provided constructive feedback as well as professional discussions, when this was needed and we owe every one of them thanks.

Especially we owe Kristian Davidsen thanks for his practical help during the project. Kristian has provided both ‘emergency’ lab help and tips and tricks for PCR. Furthermore Kristian was of great help during our BioBrick workshop.

Hilde Coumou has helped in the initial phase of our Policy & Practice work. She has introduced us to different communication strategies and how to optimise science communication, which has helped us in defining a strategy for streamlining our outreach initiatives as well as choosing our target group.

Helen Cook helped us set up the BioBrick workshop and has kindly answered all kinds of immediate questions throughout the project period.

In addition to helping us with the scientific aspects of our project and the experimental planning, Jan Martinussen and Mogens Kilstrup have opened up their lab to us so we could use their equipment for measuring fluorescence.

Ali Altintas has helped us throughout our experimental work with the BioLector, as well as with interpreting our results throughout the project. Furthermore Ali gave us the initial safety introduction to the laboratory.

Anne Egholm Pedersen has introduced us to fluorometry and helped with our flow cytometry measurements.


Acknowledgements

We are very thankful for the help and guidance that we have received from other faculty members than our own supervisors, such as

  • Sébastien Muller, for advice on work with in vitro transcription.
  • Martin Kogle provided us with competent E. coli DH5α.
  • Regina Schürmann, for helping out with laboratory facilities during the BioBrick workshop.
  • Marzanna Pulka-Amin, for materials in the laboratory
  • Marlene Dalgaard, for advice and materials for in vitro transcription.

Other iGEM Teams

During the summer an ethic workshop was hosted by the University of Copenhagen iGEM team, the outcome of the workshop can be seen here, and we are very grateful to have been invited.


Laboratories

Most of our experiments were carried out at Center for Biological Sequence Analysis (CBS) at DTU Systems Biology located at the Technical University of Denmark. Here we were invited to use their machines to perform our molecular work and measurements.

Part of the work was carried out at Metabolic Signaling and Regulation and Infection Microbiology at DTU Systems Biology. Here we used the fluorescence microscope and fluorometer to investigate our cultures, which was a great help during the project and we used the flow cytometer to measure fluorescence at cellular level.


Literature

Dr. Samie Jaffrey from Department of Pharmacology, Weill Medical College, Cornell University, was of great help, and provided us the specific sequences of their Spinach-construct which later on made the foundation for our quantification of promoter strengths.


Technical University of Denmark
Department of Systems Biology
Søltofts Plads 221
2800 Kgs. Lyngby
Denmark
+45 45 25 25 25
dtuigem14@gmail.com .

Retrieved from "http://2014.igem.org/Team:DTU-Denmark/Team/Attributions"