Team:Cambridge-JIC/Notebook/CL W4 Tuesday

From 2014.igem.org

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<h5> Ginny's day </h5>
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{{:Team:Cambridge-JIC/Templates/header}}
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<ul>
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wonders whether there's any point in filling this in... <br>
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== Ginny's day ==
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wonders whether there's any point in filling this in...  
got familiar with the new MoClo Standards and designed chromoproteins in the new biobrick compatible MoClo format <br>
got familiar with the new MoClo Standards and designed chromoproteins in the new biobrick compatible MoClo format <br>
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designed primers for the above constructs ready to send tomorrow<br>
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designed primers for the above constructs ready to send tomorrow
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read some cool stuff about potential symbiotic origin of TB<br>
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read some cool stuff about potential symbiotic origin of TB
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worked on wiki following Matt's reminders :P <br>
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worked on wiki following Matt's reminders :P
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realized how quickly time can pass...<br><br>
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realized how quickly time can pass...
Forgot to book flights... and played classical music to the lab to help us all stay sane
Forgot to book flights... and played classical music to the lab to help us all stay sane
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</ul>
 
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<h5> salil </h5>
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== salil ==
Miniprepped DNA from ecoli transformation. Messed up - added 100\mu l of buffer P2 but all was okay and DNA was successfully extracted. Good enough concentration to sequence.
Miniprepped DNA from ecoli transformation. Messed up - added 100\mu l of buffer P2 but all was okay and DNA was successfully extracted. Good enough concentration to sequence.
Ordered primers for the construct.
Ordered primers for the construct.
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Pestered others to upload progress onto the wiki.
Pestered others to upload progress onto the wiki.
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<h5>Trang-Anh</h5>
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== Trang-Anh ==
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Worked on codon optimisation: looked the results of the Blast run on the monster, for the comparison of Arabidopsis (from www.araport.org) and predicted Marchantia proteins. Wrote C++ code to extract the sequences corresponding to the matching proteins between Mpoly and Athal, from their positions in the blast output table. All relevant files are in the Dropbox under Data_mining/Codon_optimisation.
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Played around with the monster and looked at the results of the Blast run that compared Marchantia candidate proteins and Arabidopsis proteins.<br>
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Wrote C++ code to build a codon usage table from the sequences I extracted yesterday. Encountered a variety of compilation errors, segfaults and hearts, spades and smileys in my terminal.<br>
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Enjoyed Ginny's classical music.
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== Hugh ==
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More work on wiki<br>
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Reading about css to make sure our website will be attractive<br>
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Looked at good wikis from previous years for inspiration<br>
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== Angelina ==
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- Background reading and research.<br>
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- I now have a trial version of Geneious!<br>
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- Followed up Ginny’s Science Centre exhibition contact with an outreach e-mail.<br>
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{{:Team:Cambridge-JIC/Templates/CL_grid}}

Latest revision as of 11:28, 30 July 2014

Home Team Official Team Profile TimeLine Project Outreach Marchantia Informatics Parts Modeling Notebook Protocols Safety Dry Work Technology Attributions

Contents

Ginny's day

wonders whether there's any point in filling this in... got familiar with the new MoClo Standards and designed chromoproteins in the new biobrick compatible MoClo format
designed primers for the above constructs ready to send tomorrow read some cool stuff about potential symbiotic origin of TB worked on wiki following Matt's reminders :P realized how quickly time can pass...

Forgot to book flights... and played classical music to the lab to help us all stay sane

salil

Miniprepped DNA from ecoli transformation. Messed up - added 100\mu l of buffer P2 but all was okay and DNA was successfully extracted. Good enough concentration to sequence. Ordered primers for the construct. Made wiki page template for recording construct progress on the wiki. Organised DNA library/updated with concentrations of new things. Pestered others to upload progress onto the wiki.

Trang-Anh

Played around with the monster and looked at the results of the Blast run that compared Marchantia candidate proteins and Arabidopsis proteins.
Wrote C++ code to build a codon usage table from the sequences I extracted yesterday. Encountered a variety of compilation errors, segfaults and hearts, spades and smileys in my terminal.
Enjoyed Ginny's classical music.

Hugh

More work on wiki
Reading about css to make sure our website will be attractive
Looked at good wikis from previous years for inspiration

Angelina

- Background reading and research.
- I now have a trial version of Geneious!
- Followed up Ginny’s Science Centre exhibition contact with an outreach e-mail.



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