Team:NJAU China

From 2014.igem.org

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<html lang="en">
<html lang="en">
<head>
<head>
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<meta charset="utf-8">
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    <meta charset="utf-8">
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<meta http-equiv="X-UA-Compatible" content="IE=edge">
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    <meta http-equiv="X-UA-Compatible" content="IE=edge">
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<meta name="viewport" content="width=device-width, initial-scale=1">
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    <meta name="viewport" content="width=device-width, initial-scale=1">
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<meta name="description" content="">
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    <meta name="description" content="">
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<meta name="author" content="">
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    <meta name="author" content="">
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<link rel="icon" href="../../favicon.ico">
+
    <link rel="icon" href="../../favicon.ico">
-
<title>Carousel Template for Bootstrap</title>
+
    <title>Home</title>
-
<link rel="stylesheet" href="http://cdn.bootcss.com/bootstrap/3.2.0/css/bootstrap.min.css">
+
    <script src="https://2014.igem.org/Team:NJAU_China/jquery.min.js?action=raw"></script>
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<script src="http://cdn.bootcss.com/jquery/1.11.1/jquery.min.js"></script>
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    <script src="https://2014.igem.org/Team:NJAU_China/bootstrap.min.js?action=raw"></script>
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<script src="http://cdn.bootcss.com/bootstrap/3.2.0/js/bootstrap.min.js"></script>
+
-
<!-- HTML5 shim and Respond.js IE8 support of HTML5 elements and media queries -->
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    <link rel="stylesheet" type="text/css"
-
<!--[if lt IE 9]>
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          href="https://2014.igem.org/Team:NJAU_China/bootstrap.min.css?action=raw&amp;ctype=text/css">
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      <script src="https://oss.maxcdn.com/html5shiv/3.7.2/html5shiv.min.js"></script>
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    <link rel="stylesheet" type="text/css"
-
      <script src="https://oss.maxcdn.com/respond/1.4.2/respond.min.js"></script>
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          href="https://2014.igem.org/Team:NJAU_China/bootstrap-theme.min.css?action=raw&amp;ctype=text/css">
 +
    <!-- HTML5 shim and Respond.js IE8 support of HTML5 elements and media queries -->
 +
    <!--[if lt IE 9]>
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    <script src="https://oss.maxcdn.com/html5shiv/3.7.2/html5shiv.min.js"></script>
 +
    <script src="https://oss.maxcdn.com/respond/1.4.2/respond.min.js"></script>
     <![endif]-->
     <![endif]-->
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<!-- Custom styles for this template -->
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    <!-- Custom styles for this template -->
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<link href="carousel.css" rel="stylesheet">
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    <link href="carousel.css" rel="stylesheet">
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<style type="text/css">
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    <style type="text/css">
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body {
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        body {
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padding-bottom: 40px;
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            padding-bottom: 40px;
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background: #333333;
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color: #5a5a5a;
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font-size: 12px;
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            font-size: 14px;
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            font-family:Andalus;
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        #myheader{
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            width:1366px;
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    height:500px;
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    margin:0 auto;
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    position:relative;
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    font-family:Arial, Helvetica, sans-serif;
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            background:#a2dcf3 url("https://static.igem.org/mediawiki/2014/3/34/Header_bg.png");
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        #contentSub, #footer-box, #catlinks, #search-controls, #p-logo, .printfooter, .visualClear {
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        /*-- hides default wiki settings --*/
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        .firstHeading {
 +
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            padding-top: 100px;
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            margin-bottom: 20px;
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            font-family: Georgia, Times, "Andalus", serif;
 +
        }
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 +
        h1, h2, h3, h4, h5 {font-family: Georgia, Times, "Andalus", serif;}
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        #top-section {
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            /*-- styling for default menu bar (edit, page, history, etc.) --*/ background-color: #383838;border: 0 none;height: 14px;z-index: 100;top: 0;position: fixed; width: 975px;left: 50%;margin-left: -487px;}
 +
#top-section-bar {
 +
            /*-- styling full width bar which hides behind default menu bar (edit, page, history, etc.) --*/
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  background-color: #383838;
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  height: 14px;
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  display: block;
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#globalWrapper, #content {
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  height: 100%;
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  border: 0px;
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 +
  margin: 0px;
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  padding: 0px;
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 +
  html, body, .wrapper {
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  /*-- changes default wiki settings --*/
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  width: 100%;
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  height: 100%;
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  background-color: transparent;
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  } #container {
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  /*-- creates container for all content on page --*/
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  font-family: Arial, Helvetica, sans-serif;
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            font-weight: normal;
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            font-size: 14px;
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            color: #414141;
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            width: 960px;
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            margin-left: auto;
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            margin-right: auto;
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            background-color: #transparent;
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            margin-top: 0px;}
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.container {
 +
            font-size: 14px;
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            width:700px;
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            float:right;}
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.buttontext { color: #FFF}
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#my_h1{position:absolute;top:70px;left:170px;border:none;}
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#my_h1 a{display:block;width:190px;height:130px;text-indent:-9999px;}
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#my_h2{position:absolute;top:120px;left:370px;border:none;}
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#my_h2 a{display:block;width:390px;height:230px;text-indent:-9999px;}
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#my_nav{position:absolute;top:402px;left:200px;width:990px;height:50px;}
 +
#my_nav ul{width:100%;margin:0;padding:0;list-style:none;font-size:24px;}
 +
#my_nav ul li{display::inline-block;padding:9px 30px;float:left;list-style:none;position:relative;}
 +
#my_nav ul li:hover{background:#e3f7ff;}#my_nav ul li a{color:#00586b;text-decoration:none;}#project{display:none;position:absolute;z-index:100;top:47px;left:0px;background:#CF6;width:100px;}#project dd{width:135px;font-size:16px;
 +
background:#c9efff;height:40px;margin:0;}#project dd a{width:100%;height:40px;display:block;text-decoration:none;text-align:center;line-height:40px;}#project dd a:hover{background:#a1dbf0;}#achievement{display:none;position:absolute;top:47px;z-index:100;left:0px;background:#CF6;width:100px;}#achievement dd{width:198px;font-size:16px;background:#c9efff;height:40px;margin:0;}#achievement dd a{width:100%;height:40px;display:block;text-decoration:none;text-align:center;line-height:40px;}#achievement dd a:hover{background:#a1dbf0;}#prctice{display:none;position:absolute;top:47px;z-index:100;left:0px;background:#CF6;width:100px;}#prctice dd{width:300px;font-size:16px;background:#c9efff;height:40px;margin:0;}#prctice dd a{width:100%;height:40px;display:block;text-decoration:none;text-align:center;line-height:40px;}#prctice dd a:hover{background:#a1dbf0;}#ackonwledgement{display:none;position:absolute;top:47px;z-index:100;left:0px;background:#CF6;width:100px;}#ackonwledgement dd{width:257px;font-size:16px;background:#c9efff;height:40px;margin:0;}#ackonwledgement dd a{width:100%;height:40px;display:block;text-decoration:none;text-align:center;line-height:40px;}#ackonwledgement dd a:hover{background:#a1dbf0;}
 +
#my_content{
 +
width:1030px;
 +
margin:0px auto;}
 +
#my_abstract{
 +
width:300px;
 +
margin:0 0 0 25px;
 +
        float:left;
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}
 +
#my_abstract h2{
 +
height:50px;
 +
line-height:50px;
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text-indent:50px;
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border:none;
 +
background:#999;
 +
margin:0 0 20px 0;
 +
padding:0;
}
}
-
#contentSub, #footer-box, #catlinks, #search-controls, #p-logo, .printfooter, .visualClear {
+
#my_abstract p{
-
display: none;
+
text-indent:30px;
-
} /*-- hides default wiki settings --*/
+
height:240px;
-
.firstHeading {
+
overflow-y:scroll;
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width: 975px;
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margin: 0px auto;
+
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padding-top: 100px;
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-
margin-bottom: 20px;
+
-
font-family: Georgia, Times, "Times New Roman", serif;
+
}
}
-
h1, h2, h3, h4, h5 {
+
 
-
font-family: Georgia, Times, "Times New Roman", serif;
+
 
 +
 
 +
 
 +
#my_contents{
 +
width:1030px;
 +
margin:30px auto;
 +
height:800px;
}
}
-
#top-section { /*-- styling for default menu bar (edit, page, history, etc.) --*/
+
#my_con{
-
background-color: #383838;
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width:1000px;
-
border: 0 none;
+
height:796px;
-
height: 14px;
+
float:right;
-
z-index: 100;
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position:relative;
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top: 0;
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border:2px solid #999;
-
position: fixed;
+
        margin:20px 10px 0 0;
-
width: 975px;
+
-
left: 50%;
+
-
margin-left: -487px;
+
}
}
-
#top-section-bar { /*-- styling full width bar which hides behind default menu bar (edit, page, history, etc.) --*/
+
#stage_1{
-
background-color: #383838;
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width:20px;
-
height: 14px;
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height:20px;
-
display: block;
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position:absolute;
-
z-index: 10;
+
top:40px;
-
position: fixed;
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left:40px;
-
width: 100%;
+
border-left:2px solid #333;
-
top: 0;
+
border-top:2px solid #333;
}
}
-
body {
+
#stage_2{
-
background-color: #fff;
+
width:20px;
 +
height:20px;
 +
position:absolute;
 +
top:40px;
 +
left:940px;
 +
border-right:2px solid #333;
 +
border-top:2px solid #333;
}
}
-
#globalWrapper, #content { /*-- changes default wiki settings --*/
+
#stage_3{
-
width: 100%;
+
width:20px;
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+
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-
background-color: transparent;
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top:740px;
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margin: 0px;
+
left:40px;
-
padding: 0px;
+
border-bottom:2px solid #333;
 +
border-left:2px solid #333;
}
}
-
html, body, .wrapper { /*-- changes default wiki settings --*/
+
#stage_4{
-
width: 100%;
+
width:20px;
-
height: 100%;
+
height:20px;
-
background-color: transparent;
+
position:absolute;
 +
top:740px;
 +
left:940px;
 +
border-right:2px solid #333;
 +
border-bottom:2px solid #333;
}
}
-
#container { /*-- creates container for all content on page --*/
+
#real_con{
-
font-family: Arial, Helvetica, sans-serif;
+
position:absolute;
-
font-weight: normal;
+
top:60px;
-
font-size: 14px;
+
left:60px;
-
color: #414141;
+
width:880px;
-
width: 960px;
+
height:680px;
-
margin-left: auto;
+
-
margin-right: auto;
+
-
background-color: #transparent;
+
-
margin-top: 0px;
+
}
}
-
.container {
+
#real_con ul{
-
font-size:14px
+
list-style:none;
 +
width:100%;
 +
height:100%;
 +
margin:0;
 +
padding:0;
}
}
-
.buttontext {
+
#real_con ul li{
-
color:#FFF
+
width:400px;
 +
height:300px;
 +
float:left;
 +
background:#0F0;
 +
margin:26px 0 0 26px;}
 +
#real_con ul li#one_1{
 +
background:url("https://static.igem.org/mediawiki/2014/b/b4/Project_li_bg.png") no-repeat;}
 +
#real_con ul li#two_2{
 +
background:url("https://static.igem.org/mediawiki/2014/1/17/Model_li_bg.png") no-repeat;}
 +
#real_con ul li#three_3{
 +
background:url("https://static.igem.org/mediawiki/2014/6/6e/Weblab_li_bg.png") no-repeat;}
 +
#real_con ul li#four_4{
 +
background:url("https://static.igem.org/mediawiki/2014/f/f0/Practice_li_bg.png") no-repeat;}
 +
#real_con ul li a{
 +
text-decoration:none;
 +
display:block;
 +
width:100%;
 +
height:100%;
}
}
-
</style>
+
#real_con ul li a#one:hover{
-
<style>
+
background:url("https://static.igem.org/mediawiki/2014/6/68/Project_a_bg.png") no-repeat;}
-
#contentSub, #footer-box, #catlinks, #search-controls, #p-logo, .printfooter, .firstHeading, .visualClear {
+
-
display: none;
+
-
} /*-- hides default wiki settings --*/
+
-
</style>
+
-
</head>
+
-
<!-- NAVBAR
+
-
================================================== -->
+
-
<body>
+
-
    <div class="container-fluid">
+
#real_con ul li a#two:hover{
-
      <!-- Brand and toggle get grouped for better mobile display -->
+
background:url("https://static.igem.org/mediawiki/2014/d/dc/Model_a_bg.png") no-repeat;}
-
      <div class="navbar-header">
+
#real_con ul li a#three:hover{
-
        <button type="button" class="navbar-toggle collapsed" data-toggle="collapse" data-target="#bs-example-navbar-collapse-1"> <span class="sr-only">Toggle navigation</span> <span class="icon-bar"></span> <span class="icon-bar"></span> <span class="icon-bar"></span> </button>
+
background:url("https://static.igem.org/mediawiki/2014/6/60/Weblab_a_bg.png") no-repeat;}
-
        <a class="navbar-brand" href="#">Brand</a> </div>
+
#real_con ul li a#four:hover{
-
      <div class="navbar-wrapper">
+
background:url("https://static.igem.org/mediawiki/2014/7/7f/Practice_a_bg.png") no-repeat;}
-
        <div class="navbar navbar-fixed-top navbar-inverse" role="navigation">
+
-
          <div class="container">
+
-
            <div class="navbar-header">
+
-
              <button type="button" class="navbar-toggle collapsed" data-toggle="collapse" data-target=".navbar-collapse"> <span class="sr-only">Toggle navigation</span> <span class="icon-bar"></span> <span class="icon-bar"></span> <span class="icon-bar"></span> </button>
+
-
              <a class="navbar-brand" href="#">iGem 2014 NJAU_China</a> </div>
+
-
            <div class="collapse navbar-collapse">
+
-
              <ul class="nav navbar-nav">
+
-
                <li class="active"><a href="https://2014.igem.org/Team:NJAU_China" class="unselect">Home</a></li>
+
-
                <li class="dropdown"> <a href="#" class="dropdown-toggle" data-toggle="dropdown">Introduction<span class="caret"></span></a>
+
-
                  <ul class="dropdown-menu" role="menu">
+
-
                    <li><a href="#">Background</a></li>
+
-
                    <li><a href="#">Challenge</a></li>
+
-
                    <li><a href="#">Our Idea</a></li>
+
-
                  </ul>
+
-
                </li>
+
-
+
-
        <li class="dropdown"> <a href="#" class="dropdown-toggle" data-toggle="dropdown">Project<span class="caret"></span></a>
+
-
                  <ul class="dropdown-menu" role="menu">
+
-
                    <li><a href="#">Overview</a></li>
+
-
                    <li><a href="#">Part1: Sensor</a></li>
+
-
                    <li><a href="#">System</a></li>
+
-
                    <li><a href="#">Product</a></li>
+
-
                      <li><a href="#">Summary</a></li>
+
-
                  </ul>
+
-
                </li>
+
-
+
-
+
-
                <li><a href="https://2014.igem.org/Team:NJAU_China/Team"  class="unselect">Team</a></li>
+
-
                <li><a href="https://igem.org/Team.cgi?year=2014" class="unselect">Official Team Profile </a></li>
+
-
                <li><a href="https://2014.igem.org/Team:NJAU_China/Project">Project</a></li>
+
-
                <li><a href="https://2014.igem.org/Team:NJAU_China/Parts" class="unselect">Parts</a></li>
+
-
                <li><a href="https://2014.igem.org/Team:NJAU_China/Modeling" class="unselect">Modeling</a></li>
+
-
                <li><a href="https://2014.igem.org/Team:NJAU_China/Notebook" class="unselect">Notebook</a></li>
+
-
                <li><a href="https://2014.igem.org/Team:NJAU_China/Safety" class="unselect">Safety</a></li>
+
-
                <li><a href="https://2014.igem.org/Team:NJAU_China/Attributions" class="unselect">Attributions</a></li>
+
-
              </ul>
+
-
            </div>
+
-
            <!-- /.nav-collapse -->
+
-
          </div>
+
-
          <!-- /.container -->
+
-
        </div>
+
-
        <!-- /.navbar -->
+
-
       
+
-
      </div>
+
-
    </div>
+
 +
#thanks{
 +
height:177px;
 +
width:1030px;
 +
padding:30px 0 0 0;
 +
        clear:both;
 +
margin:30px auto 0;}
-
    <!-- Carousel
 
-
    ================================================== -->
 
-
<div id="myCarousel" class="carousel slide" data-ride="carousel">
 
-
    <!-- Indicators -->
 
-
    <ol class="carousel-indicators">
 
-
      <li data-target="#myCarousel" data-slide-to="0" class="active"></li>
 
-
      <li data-target="#myCarousel" data-slide-to="1"></li>
 
-
      <li data-target="#myCarousel" data-slide-to="2"></li>
 
-
    </ol>
 
-
    <div class="carousel-inner" role="listbox">
 
-
      <div class="item active text-center"> <img src="https://static.igem.org/mediawiki/2014/d/d3/Njau_slide1.jpg" alt="First slide" class="text-center">
 
-
        <div class="container">
 
-
          <div class="carousel-caption">
 
-
            <h1>Example headline.</h1>
 
-
            <p>Note: If you're viewing this page via a <code>file://</code> URL, the "next" and "previous" Glyphicon buttons on the left and right might not load/display properly due to web browser security rules.</p>
 
-
            <p><a class="btn btn-lg btn-primary" style="color:#FFF" href="#" role="button">Sign up today</a></p>
 
-
          </div>
 
-
        </div>
 
-
      </div>
 
-
      <div class="item text-center"> <img src="https://static.igem.org/mediawiki/2014/2/24/Njau_slide2.jpg" alt="Second slide">
 
-
        <div class="container">
 
-
          <div class="carousel-caption">
 
-
            <h1>Another example headline.</h1>
 
-
            <p>Cras justo odio, dapibus ac facilisis in, egestas eget quam. Donec id elit non mi porta gravida at eget metus. Nullam id dolor id nibh ultricies vehicula ut id elit.</p>
 
-
            <p><a class="btn btn-lg btn-primary" style="color:#FFF" href="#" role="button">Learn more</a></p>
 
-
          </div>
 
-
        </div>
 
-
      </div>
 
-
      <div class="item text-center"> <img src="https://static.igem.org/mediawiki/2014/6/62/Njau_slide3.jpg" alt="Third slide">
 
-
        <div class="container">
 
-
          <div class="carousel-caption">
 
-
            <h1>One more for good measure.</h1>
 
-
            <p>Cras justo odio, dapibus ac facilisis in, egestas eget quam. Donec id elit non mi porta gravida at eget metus. Nullam id dolor id nibh ultricies vehicula ut id elit.</p>
 
-
            <p><a class="btn btn-lg btn-primary" style="color:#FFF" href="#" role="button">Browse gallery</a></p>
 
-
          </div>
 
-
        </div>
 
-
      </div>
 
-
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    <p>As an common pollutant source of industry, low concentration of copper ions can do harm to many creatures. Whereas, <i>Escherichia coli</i> has high tolerance for copper through specific systems. There are <i>cop</i>A promoter,<i>mar</i>O（operator of mar locus） and <i>cue</i>O promoter existing in <i>E.Coli</i>, all of which are directly or indirectly regulated by copper ions. The most sensitive and effective one (<i>cop</i>A)was chosen to trigger the sequential reactions. Glutathione is an intracellular redox-active tripeptide thiol which can effectively detoxify the metal ions by chelation. It can be synthesized by <i>gsh</i>F, an coding sequence derived from <i>Streptococcus thermophilus</i>, which can unify both necessary reactions in one platform.In addition, we also utilize sgRNA-dCas9 system to enhance the efficiency of chelate cupric and cuprous by repressing the expression of <i>cop</i>A. With all these design, an automatically and efficiently copper-preventing guard have been created successfully. </p>
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Latest revision as of 17:03, 19 November 2014

Home

Abstract

As an common pollutant source of industry, low concentration of copper ions can do harm to many creatures. Whereas, Escherichia coli has high tolerance for copper through specific systems. There are copA promoter,marO（operator of mar locus） and cueO promoter existing in E.Coli, all of which are directly or indirectly regulated by copper ions. The most sensitive and effective one (copA)was chosen to trigger the sequential reactions. Glutathione is an intracellular redox-active tripeptide thiol which can effectively detoxify the metal ions by chelation. It can be synthesized by gshF, an coding sequence derived from Streptococcus thermophilus, which can unify both necessary reactions in one platform.In addition, we also utilize sgRNA-dCas9 system to enhance the efficiency of chelate cupric and cuprous by repressing the expression of copA. With all these design, an automatically and efficiently copper-preventing guard have been created successfully.

njaushengke

Retrieved from "http://2014.igem.org/Team:NJAU_China"