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Team:Oxford/protocols/Streaking Plates
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Latest revision as of 13:29, 23 July 2014
Streaking Plates
- Obtain an LB agar plate.
- Label the bottom of the plate.
- Carry out the following steps in the lamina flow hood. Using a sterile inoculation loop touch the top of the bacterial stock that is to be plated out.
- Gently spread the bacteria over the plate by streaking across one section of the plate. Then drag through the first section to streak over a second section of the plate. Then drag through the second section to streak the third section.
- Incubate the plate overnight at the appropriate temperature (30°C for Pseudomonas and 37°C for E. coli).
↩ Back to other protocols.
