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Team:NCTU Formosatest
From 2014.igem.org
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| - | < | + | {{:Team:NCTU Formosa/source/header-notebook}} |
| - | === | + | ===Note=== |
| - | </ | + | <ul class="cal"> |
| - | + | <li><div id="move1">April<br>2014</div></li> | |
| - | === | + | <li><div id="move2">May<br>2014</div></li> |
| + | <li><div id="move3">June<br>2014</div></li> | ||
| + | <li><div id="move4">July<br>2014</div></li> | ||
| + | <li><div id="move5">August<br>2014</div></li> | ||
| + | <li><div id="move6">September<br>2014</div></li> | ||
| + | <li><div id="move7">October<br>2014</div></li> | ||
| + | </ul> | ||
| + | <div class="doo1"> | ||
| + | ====April 2014==== | ||
| + | {{:Team:NCTU Formosa/Notebook/test}} | ||
| + | </div><div class="doo2"> | ||
| + | ====May 2014==== | ||
| + | {{:Team:NCTU Formosa/Notebook/test2}} | ||
| + | </div><div class="doo3"> | ||
| + | ====June 2014==== | ||
| + | {{:Team:NCTU Formosa/Notebook/test3}} | ||
| + | </div><div class="doo4"> | ||
| + | ====July 2014==== | ||
| + | {{:Team:NCTU Formosa/Notebook/test4}} | ||
| + | </div><div class="doo5"> | ||
| + | ====August 2014==== | ||
| + | {{:Team:NCTU Formosa/Notebook/test5}} | ||
| + | </div><div class="doo6"> | ||
| + | ====September 2014==== | ||
| + | {{:Team:NCTU Formosa/Notebook/test6}} | ||
| + | </div><div class="doo7"> | ||
| + | ====October 2014==== | ||
| + | {{:Team:NCTU Formosa/Notebook/test7}} | ||
| + | </div> | ||
Latest revision as of 13:52, 28 August 2015
Contents |
Note
- April
2014 - May
2014 - June
2014 - July
2014 - August
2014 - September
2014 - October
2014
April 2014
- Sun.
- Mon.
- Tue.
- Wed.
- Thu.
- Fri.
- Sat.
- 1
- 2
- 3
- 4
- 5
- 6
- 7
- 8
- 9
- 10
- 11
- 12
- 13
- 14
- 15
- 16
- 17
- 18
- 19
- 20
- 21
- 22
- 23
- 24
- 25
- 26
- 27
- 28
- 29
- 30
4/11
Transformation of [PSB1K3] Kr, [PSB1A3] Ar, [PSB1C3] Cr (G2)
Transformation of [Plac+PSB1A3] Ar, [mGFP+PSB1A3] Ar (G3)
Transformation of [PSB1K3] Kr,[PSB1A3] Ar, [PSB1C3] Cr (G1)
4/12
Cultivation of [PSB1K3] Kr, [PSB1C3] Cr (G2)
Cultivation of [PSB1A3] Ar, [PSB1C3] Cr (G1)
Cultivation of [mGFP] Ar (G3)
4/13
Mini-prep of [mGFP] Ar, [PSB1K3] Kr (G3)
Mini-prep of [PSB1K3] Kr, [PSB1C3] Cr (G2)
Mini-prep of [PSB1A3] Ar, [PSB1C3] Cr (G1)
4/17
Transformation of [PSB1K3] Kr (G1)
4/18
Cultivation of [PSB1K3] Kr (G1)
4/19
Transformation of [Plac+PSB1A3] Ar, [PSB1K3] Kr, [PSB1A3] Ar, [PSB1C3] Cr (G3)
Mini-prep of [PSB1K3] Kr (G1)
4/20
Transformation of [PSB1A3] Ar (G2)
Electrophoresis of [PSB1K3] Kr, [PSB1C3] Cr plasmid (G2)
4/21
Cultivation of [PSB1K3] Kr, [PSB1A3] Ar, [PSB1C3] Cr (G2)
4/22
Mini-prep of [PSB1K3] Kr, [PSB1A3] Ar, [PSB1C3] Cr (G2)
4/28
Electrophoresis of digested [PSB1K3] Kr(EP), [PSB1C3] Cr(EP),[PSB1A3] Ar(EP) (G1)
Cultivation of [PSB1C3] Cr (G1)
4/29
Mini-prep of [PSB1C3] Cr (G1)
Mini-prep of [PSB1C3] Cr (G2)
Transformation of [B0032] Ar, and [BFP] Cr (G2)
Cultivation of [PSB1K3] Kr, [PSB1A3] Ar (G1)
Cultivation of [B0032] Ar, and [BFP] Cr (G2)
4/30
Mini-prep of [PSB1A3] Ar (G1)
Mini-prep of [B0032] Ar, and [BFP] Cr (G2)
Transformation of [B0032] Ar, and [BFP] Cr, [T7promoter] Ar Kr and [T7 polymerase] Cr (G2)
Transformation of [J23101] Ar,[K592100] Cr,[B0032] Ar,[B0015] Ar (G1)
Digestion of [PSBIC3] Cr(EP), [B0032] Ar(ES), [BFP] Cr(XP) mini (G2)
Electrophoresis of plasmid and digested prouct of[PSBIC3] Cr(EP), [B0032] Ar(ES), [BFP] Cr(XP) mini (G2)
May 2014
- Sun.
- Mon.
- Tue.
- Wed.
- Thu.
- Fri.
- Sat.
- 1
- 2
- 3
- 4
- 5
- 6
- 7
- 8
- 9
- 10
- 11
- 12
- 13
- 14
- 15
- 16
- 17
- 18
- 19
- 20
- 21
- 22
- 23
- 24
- 25
- 26
- 27
- 28
- 29
- 30
- 31
5/1
Cultivation of [PSB1C3]Cr, [BFP]Cr, [T7promoter]ArKr, [T7 polymerase]Cr and [B0015]Ar (G2)
Transformation of [T7 polymerase]Cr (G2)
5/2
Cultivation of [J23101] Ar,[K592100] Cr,[B0032] Ar,[B0015] Ar (G1)
Mini-prep of [PSB1C3]Cr, [BFP]Cr, [T7promoter]ArKr, [T7 polymerase]Cr and [B0015]Ar (G2)
Digestion of [PSB1C3]Cr(EP), [BFP]Cr(XP), [T7 polymerase]Cr(ES), [T7promoter]ArKr(ES) and [B0015]Ar*2(ES & XP) (G2)
5/3
Mini-prep of [J23101] Ar,[K592100] Cr,[B0032] Ar,[B0015] Ar (G1)
Mini-pep of [B0032] Ar, [B0015] Ar,[BFP] Cr (G3)
Digestion of [J23101] Ar (ES),[K592100]Cr(ES),[B0032] Ar(XP),[B0015] Ar(XP),[PSB1A3] Ar(EP),[PSB1C3] Cr(EP),[PSB1K3] Kr(EP) mini (G1)
Electrophoresis of plasmid and digested product of [PSB1C3]Cr(EP), [BFP]Cr(XP), [T7 polymerase]Cr(ES), [T7promoter]ArKr(ES) and B0015*2(ES & XP) (G2)
Electrophoresis of digested product of [J23101] Ar (ES),[K592100] Cr(ES),[B0032] Ar(XP),[B0015] Ar(XP),[PSB1A3] Ar(EP),[PSB1C3]Cr(EP),[PSB1K3] Kr(EP) (G1)
Cultivation of [B0015] Ar, [PSB1A3] Ar, [PSB1K3] Kr (G1)
Ligation of [BFP+B0032+PSB1K3]Kr (G2)
Ligation of [J23101+B0032+PSB1C3] Cr (G1)
Transformation of ligation product [BFP+B0032+PSB1K3]Kr (G2)
Transformation of [T7polymerase]Cr and [B0015]Ar (G2)
5/4
Mini-prep of [B0015] Ar, [PSB1A3] Ar, [PSB1K3] Kr (G1)
Mini-prep of [B0015*3] Ar and [T7polymerase] Cr (G2)
Digestion of [B0015] Ar(XP), [PSB1A3] Ar(EP), [PSB1K3] Kr (EP)(G1)
Digestion of [B0015*3] Ar and [T7polymerase] Cr (G2)
Digestion of [B0015] Ar(XP), [BFP] Cr(XP), [PSB1A3] Ar(EP), [PSB1C3] Cr(EP), [B0032] Ar (XP) (G3)
Transformation of [Pcons promoter] Ar, [RBS+BFP] Kr and [T7 promoter] Kr (G2)
Transformation of [LacI] Ar (G3)
Electrophoresis of plasmid and digested product of [B0015*3] Ar and [T7polymerase]Cr (G2)
Electrophoresis of digested product of [B0015] Ar(XP), [PSB1A3] Ar(EP), [PSB1K3] Kr (EP), [K592100] Cr (ES)(G1)
Cultivation of [B0015] Ar, [PSB1A3] Ar(G1)
Cultivation of [Plac] Ar, [PSB1C3] Cr, [mGFP] Ar (G3)
5/5
Mini-prep of [PSB1A3] Ar,[B0015]Ar (G1)
Mini-prep of [Plac] Ar, [PSB1C3] Cr, [mGFP] Ar (G3)
Digestion of [PSB1A3] Ar(EP),[B0015]Ar(XP)(G1)
Electrophoresis of digested product of [PSB1A3] Ar(EP),[B0015]Ar(XP),[PSB1K3]Kr(EP) (G1)
5/6
Mini-prep of [LacI] Ar (G3)
5/7
Transformation of [PT7polymerase]Cr and [J61048]Ar (G2)
5/8
Transformation of [B0015*3] Ar and [J61048*2] Ar (G2)
Transformation of [B0015] Ar and [J61048] Ar (G3)
Transformation of [B0015]Ar,[J61048]Ar,[J23101]Ar (G1)
Mini-prep of [PT7polymerase]Cr, [T7polymerase*2]Cr and [B0015*2]Ar (G2)
Digestion of [T7polymerase] Kr (EP) and [B0015] Ar (ES) (G2)
Digestion of [Plac] Ar (ES), [mGFP] Ar (ES), [LacI] Ar (ES), [PSB1C3] Cr (EP) (G3)
Electrophoresis of digested product of [Plac] Ar (ES), [mGFP] Ar (ES), [LacI] Ar (ES), [PSB1C3] Cr (EP) (G3)
5/9
Cultivation of [B0015] Ar, [J61048] Ar [LacI] Ar (G3)
5/10
Mini-prep of [LacI] Ar (G3)
5/11
Cultivation of [J61048] Ar (G2) (ES)
Electrophoresis of plasmid and digested product of [B0015]Ar(ES), [T7polymerase] Kr(only mini) and [T7promoter] Kr (EP)
Transformation of [T7 promoter] Kr (G2)
5/12
Digestion of [T7polymerase] Kr (ES) and [J61048] Ar (ES) (G2)
5/13
Transformation of [T7 promoter] Kr (G2)
5/15
Electrophoresis of plasmid and digested product of [J61048]Ar (ES), [T7polymerase*3]Kr(ES & XP) and [PSBIK3]Kr(EP) (G2)
Cultivation of [B0015]Ar, [J61048]Ar, [J23101]Ar, [PSB1K3]Kr (G1)
Mini-prep of [PSB1K3]Kr (G1)
Digestion of [PSB1K3]Kr (EP) (G1)
5/19
Cultivation of [J23101]Ar,[B0015]Ar,[J61048]Ar (G1)
5/20
Transformation of [T7polymerase] Kr and [PSB1K3] Kr (G2)
Electrophoresis of plasmid and digested product of [T7polymerase*6] Kr and [PSB1K3*4]Kr (G2)
Mini-prep of [J23101]Ar, [B0015]Ar, [J61048]Ar (G1)
Digestion of [J23101]Ar (ES), [B0015]Ar (XP), [J61048]Ar (XP) (G1)
5/21
Digestion of [T7polymerase*6] Kr and [PSB1K3*2] Kr (G2)
Mini-prep of [T7polymerase] Kr and [PSB1K3] Kr (G2)
Electrophoresis of plasmid and digested product of [J23101]Ar(ES), [B0015]Ar(XP), [J61048]Ar(XP) and digested product [PSB1K3]Kr(EP) (G1)
Ligation of [K592100+J61048+PSB1K3] Kr (G1)
5/22
Electrophoresis of plasmid and digested product of [T7polymerase] Kr (ES) and [PSB1K3]Kr (EP) (G2)
5/23
Transformation of ligation product [K592100+J61048+PSB1K3]Kr (G1)
5/24
Digestion of [J61048] Ar (XP) (G2)
PCR of [BFP+B0032*4] Kr and [T7 promoter*1] Ar Kr (G2)
Electrophoresis of PCR products [BFP+B0032*4] Kr and [T7 promoter*1] Ar Kr, [J61048] Ar (G2)
5/27
Electrophoresis of digested product of [mGFP] Ar (ES), [J61048] Ar (XP) ,[B0015] Ar (XP) (G3)
Digestion of [J61048] Ar (XP) and [T7polymerase] Kr (ES) (G2)
Cultivation of [PSB1K3*3] Kr(G2)
5/28
PCR of [PSBIK3*5]Kr , [PSBIA3*5]Ar , [PSBIC3*5]Cr ,[J61048*5]Ar ,[B0015*5]Ar and [BFP*5](G1)
Mini-prep of [PSB1K3*3] Kr(G2)
Digestion of [PSB1K3*3] Kr (EP) (G2)
Electrophoresis of plasmid and digested product of [PSB1K3] Kr (EP), [T7polymerase] Kr (ES), [J61048] Ar (XP) (G2)
5/29
Digestion of [mGFP] Ar (ES), [J61048] Ar (XP) ,[B0015] Ar (XP) (G3)
Transformation of [J23101] Ar (G1)
Transformation of [T7 promoter] Ar Kr (G2)
Electrophoresis of plasmid and digested product of [J61048] Ar (ES), [T7polymerase] Kr (ES) (G2)
Ligation of [J61048+J61048] Cr, [T7polymerase+J61048] Kr, [RBS+BFP] Kr (G2)
Ligation of [Plac+B0032+PSB1K3] Kr (G3)
5/30
Transformation of ligation product (G2)
Transformation of ligation product [Plac+B0032+PSB1K3] Kr (G3)
June 2014
- Sun.
- Mon.
- Tue.
- Wed.
- Thu.
- Fri.
- Sat.
- 1
- 2
- 3
- 4
- 5
- 6
- 7
- 8
- 9
- 10
- 11
- 12
- 13
- 14
- 15
- 16
- 17
- 18
- 19
- 20
- 21
- 22
- 23
- 24
- 25
- 26
- 27
- 28
- 29
- 30
6/2
PCR of [T7 promoter*4] Ar Kr, [J61048+J61048*3] Cr, [T7polymerase+J61048*3] Kr and [B0032+BFP*3] Kr (G2)
Cultivation of [T7 promoter*4] Ar Kr, [J61048+J61048*3] Cr, [T7polymerase+J61048*3] Kr and [B0032+BFP*3] Kr (G2)
Electrophoresis of [T7 promoter*4] Ar Kr, [J61048+J61048*3] Cr, [T7polymerase+J61048*3]Kr and [B0032+BFP*3] Kr plasmid (G2)
6/3
PCR of [T7 promoter*5] Ar Kr, [B0032+BFP*5] Kr , [J61048+J61048*5] Cr and [T7polymerase+J61048*5] Kr (G2)
Electrophoresis of PCR products (G2)
Cultivation of [B0032+BFP*10] Kr, [J61048+J61048*10] Cr and [T7polymerase+J61048*5] Kr (G2)
6/5
Transformation of ligation product [K592100+J61048+PSBIK3] Kr (G1)
Ligation of [BFP+B0032] Kr and [T7polymerase+J61048] Kr (G2)
Ligation of [mGFP+B0015+PSB1K3] Kr and [mGFP+J61048+PSB1K3] Kr (G3)
6/6
Mini-prep of [J61048+J61048*3] Cr and [T7 promoter*3]Ar Kr (G2)
Digestion of [J61048+J61048*3] Cr (XP) and [T7 promoter*3]Ar Kr(ES) (G2)
Transformation of [B0032+BFP] Kr and [T7polymerase+J61048] Kr (G2)
Transformation of ligation product [mGFP+B0015+PSB1K3] Kr and [mGFP+J61048+PSB1K3] Kr (G3)
6/7
PCR of [PSB1K3*6] Kr, [K592100+J61048+PSB1K3*10] Kr,[J23101*8] Ar ,[B0032*8] Ar (G1)
PCR of [mGFP+B0015+PSB1K3*6] Kr and [mGFP+J61048+PSB1K3*6] Kr (G3)
6/8
Electrophoresis of plasmid and digestion product of [J61048+J61048](XP), [T7 promoter](ES)
PCR of [B0032+BFP*5] Kr and [T7polymerase+J61048*5] Kr (G2)
Cultivation of [B0032+BFP*5] Kr and [T7polymerase+J61048*5] Kr (G2)
Electrophoresis of [B0032+BFP*5] Kr and [T7polymerase+J61048*5] Kr (G2)
6/9
Transformation of [B0023*2 ] Ar ,[J23101*2] Ar, [K592100+J61048*2] Kr and [PSB1K3*2] Kr (G1)
Electrophoresis of plasmid and digestion product of [RBS+BFP]Kr (ES),[T7 polymerase+J61048]Kr(XP),[T7 promoter]Kr Cr (ES),[J61048+J61048]Cr(XP) (G2)
Mini-prep of the plasmid and then digested the plasmid of [T7 polymerase+J61048]Kr(XP),[RBS+BFP]Kr(ES) (G2)
6/10
Electrophoresis of plasmid and digestion product of [K592100+J61048*2] Kr (XP), [PSB1K3*2] Kr (EP),[J23101*2] Ar (SP) and [B0023*2 ] Ar (SP) (G1)
6/11
Digestion of [mGFP] Ar (ES), [J61048] Ar (SP) ,[B0015] Ar (SP) (G3)
6/12
Ligation of [mGFP+B0015+PSB1K3] Kr and [mGFP+J61048+PSB1K3] Kr (G3)
PCR of [Plac+B0032+PSB1K3*12] Kr (G3)
6/13
Transformation of ligation product [mGFP+B0015+PSB1K3] Kr and [mGFP+J61048+PSB1K3] Kr (G3)
Cultivation of [Plac+B0032+PSB1K3*4] Kr (G3)
6/14
Mini-prep of [Plac+B0032+PSB1K3*2] Kr (G3)
Cultivation of [Plac+B0032+PSB1K3*4] Kr (G3)
PCR of [mGFP+B0015+PSB1K3*6] Kr
6/15
Mini-prep of [Plac+B0032+PSB1K3*4] Kr (G3)
Digestion of [Plac+B0032+PSB1K3*4] Kr (ES) (G3)
6/24
Digestion of [J23101] Ar (ES) and [B0032] Ar (XP) (G1)
Ligation of [B0032+K592100+J61048] Ar (G1)
Electrophoresis of plasmid and digestion product of [K592100+J61048] Kr (XP), [J23101] Ar (SP) and [B0032] Ar (SP) (G1)
Digestion of [T7 polymerase +J61048]Kr(XP),[J61048+J61048]Cr(XP) (G2)
6/27
Digestion of [J61048+J61048] Cr (XP) (G2)
Electrophoresis of [RBS+BFP]kr(ES)(mini),[T7polymerase](ES)(mini),[T7 promoter](mini)
6/28
Transformation of mini [B0032+BFP+J61048] Cr (G2)
6/29
PCR of [B0032+BFP+J61048]Cr
Electrophoresis of the PCR products (G2)
July 2014
- Sun.
- Mon.
- Tue.
- Wed.
- Thu.
- Fri.
- Sat.
- 1
- 2
- 3
- 4
- 5
- 6
- 7
- 8
- 9
- 10
- 11
- 12
- 13
- 14
- 15
- 16
- 17
- 18
- 19
- 20
- 21
- 22
- 23
- 24
- 25
- 26
- 27
- 28
- 29
- 30
- 31
7/1
Ligation of [J23101+B0032+PSB1C3] Cr (G1)
Digestion of [B0032] Ar
Electrophoresis of plasmid and digestion products of[B0032+BFP+J61048]Cr (XP) (G2)
7/2
Transformation of ligation product [B0032+K592100+J61048] Ar and [J23101+B0032] Cr (G1)
Cultivation of [B0032+BFP+J61048] Cr in LB broth (G2)
7/3
Mini-prep of [B0032+BFP+J61048] and then digestion: (XP)
Electrophoresis of plasmid and digestion products of[B0032+BFP+J61048]Cr (XP) (G2)
7/4
Digestion of [T7 promoter](SP) (G2)
Cultivation of [J23101] Ar (G3)
7/5
PCR of [B0032+K592100+J61048*11] Ar (G1)
Electrophoresis of PCR products of(G1)
Ligation of [J23101+B0032+PSB1C3*3] Cr (G1)
Electrophoresis of plasmid and digestion products of[T7promoter](SP),[B0031+BFP+J61048](XP)
Ligation of [T7 promoter])(SP)+[B0032+BFP+J61048](XP) (G2)
7/6
Transformation of the ligation products made at 7/5 (G2)
7/7
Transformation of [J23101+B0032*5] Cr (G1)
Cultivation of [B0032+K592100+J61048*2] Ar (G1)
Cultivation of [J23101] Ar (G3)
Digestion of [B0015] Ar (XP), [J61048] Ar (XP) (G3)
7/8
Mini-prep of [B0032+K592100+J61048*2] Ar and then digest of only one of two: (XP) (G1)
PCR of [T7+B0032+BFP+J61048] (G2)
Digestion of [J23101] Ar (ES) (G3)
7/9
Cultivation of [B0032+BFP+J61048]Cr and [T7+B0032+BFP+J61048]Kr(G2)
7/10
Mini-prep of [B0032+BFP+J61048]Cr
Digestion of [B0032+FP+J61048]Cr( XP)
Electrophoresis of plasmid and digestion products of[B0032+BFP+J61048]Cr (XP) (G2)
7/12
Digestion of [B0032+BFP+J612048](XP), [T7+B0032+BFP+J61048]Kr (XP) (G2)
7/14
Electrophoresis of [B0032+BFP+J61048]Cr,[T7+B0032+BFP+J61048]Kr (G2)
7/15
Cultivation of [B0032+BFP+J61048]Cr,[PT7+B0032+BFP+J61048]Kr E.coli in LB broth (G2)
7/16
Mini-prep of [B0032+BFP+J61048],[PT7+B0032+BFP+J61048]
Digestion of [B0032+BFP+J61048]Cr(XP),[PT7+B032+BFP+J61048]Kr(ES) (G2)
7/17
Electrophoresis of the plasmid and digestion products made yesterday (G2)
7/19
PCR of [J23101+B0032*12] Cr (G1)
Electrophoresis of PCR products of [B0032+K592100+J61048*2] Ar ,digestion product of [B0032+K592100+J61048] Ar (XP) and [J23101] Ar (SP) (G1)
7/20
Digestion of [B0032+K592100+J61048] Ar (EP) (G1)
Digestion of [J23101] Ar (ES) (G3)
Ligation of [B0032+K592100+J61048] Cr (G1)
Ligation of [J23101+B0032+PSB1K3] Kr (G3)
PCR of [J23101+B0032*4] Cr (G1)
Cultivation of [J61048+B0032*4]Cr E.coli in LB broth (G1)
Electrophoresis of the PCR products and digestion products of [J23101] Ar (SP) and [B0032+K592100+J61048] Ar (EP) (G1)
7/21
Transformation of ligation product made yesterday (G1)
Transformation of ligation product of [J23101+B0032+PSB1K3] Kr (G3)
Ligation of [mGFP+J61048+PSB1K3] Kr,[mGFP+B0015+PSB1K3] Kr (G3)
7/23
Cultivation of [B0032+K592100+J61048] Cr E.coli in LB broth (G1)
Digestion of [J23101+B0032*3] Cr (EP) (G1)
Digestion of [B0032+K592100+J61048] Ar (EP) (G1)
Ligation of [J23101+B0032] Cr (G1)
Mini-prep of [B0032+K592100+J61048] Cr
Transformation of [B0032+K592100+J61048] Cr and [J23101+B0032] Ar (G1)
7/24
PCR of [B0032+K592100+J61048*12] Cr (G1)
Electrophoresis of digestion products of[J23101+B0032*3] Cr (EP) and the PCR products (G1)
Transformation of [J23101+B0032] Cr (G1)
Digestion of [BFP] Cr (ES), [B0015] Ar (XP), [J61048] Ar (XP), [B0032] Ar (XP), [BFP+B00315*2] Kr (XP) (G3)
7/26
Cultivation of [J23101+B0032*4] Cr E.coli in LB broth (G1)
Digestion of [J23101] Ar (SP), [B0032] Ar (XP) (G3)
7/27
Ligation of [J23101+B0032+PSB1C3] Cr (G3)
7/28
Digestion of [B0032+K592100+J61048*2] Cr (XP) (G1)
Transformation of [J23101] Ar (G3)
PCR of [J23101+B0032+PSB1C3*6] Cr (G3)
7/29
Electrophoresis of the digestion products of[B0032+K592100+J61048*2] Cr (XP) , [PSB1K3*2] Kr (EP) , [PSB1A3*3] Ar (EP) , [PSB1C3] Cr (EP) , [J23101] Ar (ES) , [B0032] Ar (SP) , [B0032] Ar (XP) , [B0032+K592100+J61048] Ar (XP) and [J23101] Ar (SP) (G1)
Electrophoresis of the digestion product of [B0032] Ar (EP),[PSB1C3] Cr (EP), [PSB1K3] Kr (EP), [PSB1A3] Ar (EP) and PCR product [J23101*6] Ar (G3)
Digestion of [B0032+K592100+J61048*2] Cr (XP) (G1)
Digestion of [B0032] Ar (EP), [PSB1C3] Cr (EP), [PSB1K3] Kr (EP), [PSB1A3] Ar (EP) (G3)
Mini-prep of [PT7+B0032+BFP+J61048] (G2)
PCR of [J23101*6] Ar (G3)
7/30
Electrophoresis of the digestion products of [B0032+K592100+J61048*2] Cr (XP) , [PSB1K3*2] Kr (EP) , [PSB1A3*3] Ar (EP) , [PSB1C3] Cr (EP) , [J23101] Ar (ES) , [B0032] Ar (SP) , [B0032] Ar (XP) , [B0032+K592100+J61048] Ar (XP) and [J23101] Ar (SP) (G1)
Electrophoresis of the plasmid and digestion products of [RBS],[B0032+BFP+J61048](XP),[PT7+B0032+BFP+J61048](XP) (G2)
7/31
Digestion of [B0032+K592100+J61048*2] Cr (XP) and [J23101] Ar (EP) (G1)
Ligation of [J23101+B0032+PSB1C3] Cr (G1)
Digestion of [B0032+K592100+J61048*2] Cr (XP) (G1)
Electrophoresis of the plasmid and digestion products of [B0032+K592100+J61048*2] Cr (XP) ,digestion products made in 7/7 and 7/30[B0032+K592100+J61048*2] Cr (XP) ; digestion product made in 7/31[B0032+K592100+J61048*2] Ar (XP) (G1)
August 2014
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8/11
Transformation of NCTU1 (G1)
Transformation of PBAN (G2)
8/12
Cultivation of [PBAN-4]Kr,[PBAN-5]Krand [PBAN-6]Kr E.coli in LB broth (G1)
Cultivation of [RBS+BFP+J61048]Cr ,[T7+RBS+BFP+J61048]Ar Kr
Mini-prep of [Pcons]Ar
Digestion of [Pcons]Ar (ES) (G2)
8/13
Mini-prep of [PBAN-4] Kr,[PBAN-5] Krand [PBAN-6] Kr (G1)
Digestion of [PBAN-4]Kr(EP),[PBAN-5]Kr(EP)and [PBAN-6]Kr(EP) (G1)
Electrophoresis of [Pcons](mini)&(dig ES),digestion of [Pcons](XP)
Ligation of [Pcons]+[RBS+BFP+J61048] (G2)
8/14
Electrophoresis of plasmid and digestion products of [PBAN-4] Kr(EP),[PBAN-5] Kr(EP)and [PBAN-6] Kr(EP) (G1)
Purification from electrophoresis of [PBAN]No.4&5&6(G1)
Transformation of the ligation product made yesterday
Electrophoresis of plasmid and digestion products of [PBAN]No.4&5&6,[Pcons],[Pcons+RBS+BFP+J61048] (G2)
8/15
Digestion of purification products yesterday:[PBAN]No.4&5&6 (EP) (G1)
Purification from electrophoresis of [PBAN]No.4&5&6
Transformation of [B0034]Ar (G2)
8/16
Electrophoresis of the purification products from electrophoresis,digestion products of[PBAN]No.4&5&6 (EP) (G1)
PCR of [B0034]
Electrophoresis of [PSB1K3](mini)&(dig E),[Pcons](dig SP),PCR products of B0034
Mini-prep of [B0034]Ar E.coli
Ligation of [Pcons]+[B0034] +PSB1K3
Digestion of [BFP](ES)
Electrophoresis of plasmid and digestion products of [PSB1K3],[PSB1C3],5/12[J61048],[Pcons])
8/17
Electrophoresis of the digestion products of[PBAN]No.4&5&6 (EP) (G1)
September 2014
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9/4
Cultivation of
Pcons+RBS+AseI PCR product-1,2,3,4,7,8 Ar
XhoI+PSB1K3 PCR product-1,2 Kr
PSB1A3 Ar
Cultivation plate test
Ar plate and Kr plate
Digestion of
[PBAN-HindIII] Kr*2 (EP)
[PBAN-NheI]Kr*2 (EP)
[PBAN-KpnI-HF]Kr*2 (EP)
[23101+B0034+PBAN (HindIII)]Ar(ES)
[23101+B0034+PBAN (NheI)]Ar(ES)
PCR of
[J23101+B0034+KpnI-HF] Ar*3
Ligation of
[J23101+B0034+KpnI-HF+PSB1C3]
Electrophoresis of
[PBAN-HindIII] Kr*2 (EP)
[PBAN-NheI]Kr*2 (EP)
[PBAN-KpnI-HF]Kr*2 (EP)
[23101+B0034+PBAN (HindIII)]Ar(ES)
[23101+B0034+PBAN (NheI)]Ar(ES)
[23101+B0034+PBAN (HindIII)]Ar(EP)*2
[23101+B0034+PBAN (NheI)]Ar(EP)
[23101+B0034+PBAN (KpnI-HF)]Ar(EP)*2
[23101+B0034+PBAN (KpnI-HF)]Ar PCR*3
9/5
Electrophoresis of
Well 1 Pcons+RBS+AseI--1 (EP) Ar
Well 2 Pcons+RBS+AseI--2 (EP) Ar
Well 3 Pcons+RBS+AseI--3 (EP) Ar
Well 4 Pcons+RBS+AseI--4 (EP) Ar
Well 5 Pcons+RBS+AseI--7 (EP) Ar （DNA sequencing）
Well 6 Pcons+RBS+AseI--8 (EP) Ar
Well 7 Pcons+RBS+NcoI--2 (EP) Cr （DNA sequencing）
Well 8 Pcons+RBS+Ndel--2 (EP) Cr （DNA sequencing）
Well 9 1 Kb marker
Well 10 Pcons+RBS+Ndel--13 (EP) Cr
Well 11 SacI--3 (XP) Kr
Well 12 SacI--4.1 (XP) Kr
Well 13 SacI--4.2 (XP) Kr
Well 14 XhoI--7 (EP) Kr （DNA sequencing）
Well 15 XhoI--8 (EP) Kr（DNA sequencing）
Well 16 SalI--3 (XP) Kr
Mini-prep of
Pcons+RBS+KpnI-HF-7
Pcons+RBS+KpnI-HF-8
Pcons+RBS+KpnI-HF-9
Digestion of
Pcons+RBS+KpnI-HF-7 (EP)
Pcons+RBS+KpnI-HF-8 (EP)
Pcons+RBS+KpnI-HF-9 (EP)
Cultivation of
Pcons+RBS+KpnI-HF Ar PCR product-7,8,9 Ar
Ligation of
[J23101+B0034+HindIII+B0034+PSB1K3] Kr
[J23101+B0034+HindIII+J61048+PSB1K3] Kr
[J23101+B0034+NheI+B0034+PSB1K3] Kr
[J23101+B0034+NheI+J61048+PSB1K3] Kr
Electrophoresis of digested product of
[Pcons+RBS+KpnI-HF]Ar (EP)*3
9/9
Cultivation of
Pcons+RBS+SacI PCR product-16 Ar
Pcons+RBS+SaII PCR product-17 Ar
RBS+BFP+Ter PCR product-16,17,29,30 Ar
Digestion of
XhoI+PSB1K3 (EP)
9/10
Mini-prep of
RBS+BFP+Ter-16 Ar
RBS+BFP+Ter-17 Ar
RBS+BFP+Ter-29 Ar
RBS+BFP+Ter-30 Ar
Pcons+RBS+Sac-16 Ar
Pcons+RBS+Sal-17 Ar
Digestion of
RBS+BFP+Ter-16 Ar(EP)
RBS+BFP+Ter-17 Ar(EP)
RBS+BFP+Ter-29 Ar(EP)
RBS+BFP+Ter-30 Ar(EP)
Pcons+RBS+SacI-16 Ar(EP)
Pcons+RBS+SalI-17 Ar(EP)
Electrophoresis of
Well 1 Marker
Well 2 Pcons+RBS+SacI-16 dig(EP)
Well 3 Pcons+RBS+SalI-17 dig(EP)
Well 4 RBS+BFP+Ter-16 dig(EP)
Well 5 RBS+BFP+Ter-17 dig(EP)
Well 6 RBS+BFP+Ter-29 dig(EP)
Well 7 RBS+BFP+Ter-30 dig(EP)
Well 8 XhoI-7 dig(XP) Kr
9/11
Digestion of
Pcons+RBS+PBAN(ES)(8 kind except of XhoI)
RBS+BFP+Ter(XP)
Ligation of
Pcons+RBS+XhoI-7 Ar
Cultivation of
Pcons+RBS+AseI-7 Ar
Pcons+RBS+SacI-16 Ar
Pcons+RBS+SalI-17 Ar
Pcons+RBS+NcoI-2 Ar
Pcons+RBS+NdeI-2 Ar
RBS+BFP+Ter-2 Ar
RBS+BFP+Ter-2 Ar
SalI-3 Kr
Ligation of
Pcons+RBS+XhoI-7 Ar
DNA sequencing of
Pcons+RBS+SaeI Ar 8ul VF2
Pcons+RBS+SalI Ar 8ul VF2
RBS+BFP+Ter-29 Ar 8ul VF2 + 8ul VR
RBS+BFP+Ter-30 Ar 5ul VF2 + 5ul VR
Digestion of
Pcons+RBS+HindIII dig(EP)
Pcons+RBS+NheI dig(EP)
Pcons+RBS+NcoI dig(EP)-->9/11 mini
Pcons+RBS+NdeI dig(EP)
Pcons+RBS+SacI dig(EP)
Pcons+RBS+SalI dig(EP)
Pcons+RBS+AseI dig(EP)
Pcons+RBS+KpnI dig(EP)
Ligation of
Pcons+RBS+BFP+Ter-30 Kr
9/12
Transformation of
Pcons+RBS+NheI+RBS+BFP+Ter Kr
Pcons+RBS+NdeI+RBS+BFP+Ter Kr
Pcons+RBS+HindIII+RBS+BFP+Ter Kr
Pcons+RBS+KpnI-HF+RBS+BFP+Ter Kr
Pcons+RBS+AseI+RBS+BFP+Ter Kr
Pcons+RBS+SacI+RBS+BFP+Ter Kr
Pcons+RBS+NcoI+RBS+BFP+Ter Kr
Pcons+RBS+SalI+RBS+BFP+Ter Kr
Pcons+RBS+BFP+Ter Kr
Pcons+RBS+XhoI Ar
Electrophoresis of
Well 1 Marker
Well 2 Pcons+RBS+NdeI dig(EP) Cr
Well 3 Pcons+RBS+NcoI dig(EP) Cr
Well 4 Pcons+RBS+SacI dig(EP) Ar
Well 5 Pcons+RBS+SalI dig(EP) Ar
Well 6 Pcons+RBS+AseI dig(EP) Ar
Well 7 Pcons+RBS+HindIII dig(EP) Ar
Well 8 Pcons+RBS+NheI dig(EP) Ar
Well 9 Pcons+RBS+KpnI dig(EP) Ar
9/13
Ligation of
Pcons+RBS+AseI Kr
Pcons+RBS+NdeI Kr
Pcons+RBS+SalI Kr
Pcons+RBS+SacI Kr
PCR of
Pcons+RBS+HindIII+RBS+BFP+Ter Kr x8
Pcons+RBS+NheI+RBS+BFP+Ter Kr x8
Pcons+RBS+NdeI+RBS+BFP+Ter Kr x8
Pcons+RBS+NcoI+RBS+BFP+Ter Kr x8
Pcons+RBS+KpnI-HF+RBS+BFP+Ter Kr x8
Pcons+RBS+AseI+RBS+BFP+Ter Kr x8
Pcons+RBS+Xhol Kr x8
Pcons+RBS+SalI+RBS+BFP+Ter Kr x16
Pcons+RBS+SacI+RBS+BFP+Ter Kr x16
Electrophoresis of
Gel 1
Pcons+RBS+SalI+RBS+BFP+Ter Kr x16
Gel 2
Pcons+RBS+SacI+RBS+BFP+Ter Kr x16
Gel 3
Pcons+RBS+HindIII+RBS+BFP+Ter Kr x8
Pcons+RBS+NheI+RBS+BFP+Ter Kr x8
Gel 4
Pcons+RBS+NdeI+RBS+BFP+Ter Kr x8
Pcons+RBS+NcoI+RBS+BFP+Ter Kr x8
Gel 5
Pcons+RBS+KpnI-HF+RBS+BFP+Ter Kr x8
Pcons+RBS+AseI+RBS+BFP+Ter Kr x8
Gel 6
Pcons+RBS+Xhol Kr x8
psb1K3 dig(EP)
9/16
Transformation of
psb3K3 *2
psb3C3
Digestion of
psb3K3 dig(EP)
psb1C3 dig(EP)
Cultivation of
Pcons+RBS+PBAN+RBS+BFP+Ter x8
9/17
Mini-prep of
PBAN：XhoI
SalI
SacI
KpnI
Ndel
AseI
NcoI
NheI
HindIII (fail)
Pcons+RBS+PBAN+RBS+BFP+Ter：AseI-4
Ndel-8
HindIII-4 (fail)
SacI-9(fail)
KpnI-1
NcoI-2
NheI-1
Digestion of
PBAN：XhoI
SalI
SacI
KpnI
Ndel
AseI
NcoI
AseI
Pcons+RBS+PBAN+RBS+BFP+Ter：AseI-4
Ndel-8
KpnI-1
NcoI-2
NheI-1
Electrophoresis of
Pcons+RBS+PBAN+RBS+BFP+Ter (Ndel)
Pcons+RBS+PBAN+RBS+BFP+Ter (NcoI)
Pcons+RBS+PBAN+RBS+BFP+Ter (SacI)
Pcons+RBS+PBAN+RBS+BFP+Ter (HindIII)
Pcons+RBS+PBAN+RBS+BFP+Ter (SalI)
Pcons+RBS+PBAN+RBS+BFP+Ter (NheI)
Pcons+RBS+PBAN+RBS+BFP+Ter (KpnI)
Pcons+RBS+PBAN+RBS+BFP+Ter (AseI)
PBAN AseI
PBAN Ndel
PBAN KpnI
PBAN SacI
PBAN SalI
PBAN XhoI
Pcons+RBS+XhoI-1 dig(EP)
Pcons+RBS+Xhol-1 dig(ES)
psb1C3 dig(ES)
Cultivation of
Pcons+RBS+PBAN+RBS+BFP+Ter (SacI)-4.12
Pcons+RBS+PBAN+RBS+BFP+Ter (HindIII)-6
Pcons+RBS+PBAN+RBS+BFP+Ter (SalI)-9
Digestion of
B0034 Kr dig(ES)
Pcons+RBS+AseI+RBS+BFP+Ter Kr dig(EP)
Pcons+RBS+NdeI+RBS+BFP+Ter Kr dig(EP)
NcoI Kr dig(EP)
Ndel Kr dig(EP)
SalI Kr dig(EP)
9/18
Electrophoresis of
Well 1 Marker
Well 2 B0034 Kr dig(ES)
Well 3 Pcons+RBS+AseI+RBS+BFP+Ter Kr dig(EP)
Well 4 Pcons+RBS+NdeI+RBS+BFP+Ter Kr dig(EP)
Well 5 NcoI Kr dig(EP)
Well 6 Ndel Kr dig(EP)
Well 7 SalI Kr dig(EP)
Electrophoresis of
Well 1 Marker
Well 2 B0034 Kr dig(ES)
Well 3 Pcons+RBS+AseI+RBS+BFP+Ter Kr dig(EP)
Well 4 Pcons+RBS+NdeI+RBS+BFP+Ter Kr dig(EP)
Well 5 NcoI Kr dig(EP)
Well 6 Ndel Kr dig(EP)
Well 7 SalI Kr dig(EP)
Mini-prep of
Pcons+RBS+HindIII+RBS+BFP+Ter-6
Pcons+RBS+SalI+RBS+BFP+Ter-9
Pcons+RBS+SacI+RBS+BFP+Ter-4
Pcons+RBS+SacI+RBS+BFP+Ter-11
Ligation of
Pcons+RBS+AseI+RBS+BFP+Ter Cr
Pcons+RBS+NdeI+RBS+BFP+Ter Cr
NheI Cr
NcoI Cr
9/20
Transformation of
AseI mini Cr
Electrophoresis of
Well 1 Marker
Well 2 HindIII-2 Kr dig(EP)
Well 3 SacI Kr dig(EP)
Well 4 Pcons+RBS+HindIII-6+RBS+BFP+Ter Kr dig(EP)
Well 5 Pcons+RBS+SacI-11+RBS+BFP+Ter Kr dig(EP)
Well 6 Pcons+RBS+SacI-9+RBS+BFP+Ter Kr dig(EP)
Well 7 RBS+BFP+Ter Ar dig(XP)
Well 8 Pcons+RBS+NheI+RBS+BFP+Ter Kr dig(EP)
9/21
Electrophoresis of PCR products of
Gel 1
Well 1 Marker
Well 2 3H1
Well 3 3H2
Well 4 3H3
Well 5 3H4
Well 6 3H5
Well 7 3H6
Well 8 Marker
Well 9 6K1
Well 10 6K2
Well 11 6K3
Well 12 6K4
Well 13 6K5
Well 14 6K6
Well 15 3Sl1
Well 16 3Sl2
Well 17 3Sl3
Gel 2
Well 1 Marker
Well 2 6Sl4
Well 3 6Sl5
Well 4 3Nh1
Well 5 3Nh2
Well 6 3Nh3
Well 7 3Nh4
Well 8 Marker
Well 9 3Nh5
Well 10 3Nh6
Well 11 6X1
Well 12 6X2
Well 13 6X3
Well 14 6X4
Well 15 6X5
Well 16 6X6
Well 17 1K1
Gel 3
Well 1 Marker
Well 2 1K2
Well 3 1K3
Well 4 1K4
Well 5 1K5
Well 6 1K6
Well 7 1X1
Well 8 Marker
Well 9 1X2
Well 10 1X3
Well 11 1X4
Well 12 1X5
Well 13 1X6
Well 14 3K1
Well 15 3K2
Well 16 3K3
Well 17 3K4
Gel 4
Well 1 Marker
Well 2 3K5
Well 3 3K6
Well 4 3Sc1
Well 5 3Sc2
Well 6 3Sc3
Well 7 3Sc4
Well 8 Marker
Well 9 3Sc5
Well 10 3Sc6
Well 11 1Sc1
Well 12 1Sc2
Well 13 1Sc3
Well 14 1Sc4
Well 15 1Sc5
Well 16 1Sc6
Well 17 1Nc1
Gel 5
Well 1 Marker
Well 2 1Nc2
Well 3 1Nc3
Well 4 1Nc4
Well 5 1Nc5
Well 6 1Nc6
Well 7 1A1
Well 8 Marker
Well 9 1A2
Well 10 1A3
Well 11 1A4
Well 12 1A5
Well 13 1A6
Well 14 1Nd1
Well 15 1Nd2
Well 16 1Nd3
Well 17 1Nd4
Gel 6
Well 1 Marker
Well 2 1Nd5
Well 3 1Nd6
Well 4 3X1
Well 5 3X2
Well 6 3X3
Well 7 3X4
Well 8 Marker
Well 9 3X5
Well 10 3X6
Well 11 3A1
Well 12 3A2
Well 13 3A3
Well 14 3A4
Well 15 3A5
Well 16 3A6
Cultivation of
KpnI-1
NdeI-1
XhoI-1
SacI-1
AseI-1
Pcons+RBS+HindIII-1
Pcons+RBS+KpnI-4
Pcons+RBS+NheI-4
Pcons+RBS+SalI-5
Pcons+RBS+AseI-5
Pcons+RBS+XhoI-1
Pcons+RBS+SacI-1
Pcons+RBS+KpnI-1+RBS+BFP+Ter
Pcons+RBS+XhoI-6+RBS+BFP+Ter
Digestion of
SalI Kr dig(EP)
Ligation of
NdeI dig(EP)+C backbone
9/22
Transformation of
NheI Cr
Pcons+RBS+NheI+RBS+BFP+Ter Cr
Pcons+RBS+NcoI+RBS+BFP+Ter Cr
Pcons+RBS+Ndel+RBS+BFP+Ter Cr
Pcons+RBS+AseI+RBS+BFP+Ter Cr
Digestion of
HindIII(EP)
Cultivation of
SacI-3 Kr
NcoI-7 Cr
NcoI-8 Cr
Pcons+RBS+SacI-2
Pcons+RBS+XhoI-5+RBS+BFP+Ter
pbs3C5
Ligation of
Pcons+RBS+NcoI+RBS+BFP+Ter Cr
Pcons+RBS+HindIII+RBS+BFP+Ter Cr
Pcons+RBS+SacI+RBS+BFP+Ter Cr
Pcons+RBS+SalI+RBS+BFP+Ter Cr
9/23
Electrophoresis of
Well 1 Marker
Well 2 KpnI-1 Cr dig(EP)
Well 3 NdeI-1 Cr dig(EP)
Well 4 XhoI-1 Cr dig(EP)
Well 5 SacI-1 Cr dig(EP)
Well 6 AseI-1 Cr dig(EP)
Well 7 Pcons+RBS+HindIII-1 dig(EP)
Well 8 Pcons+RBS +KpnI-4 dig(EP)
Well 9 Pcons+RBS+NheI-4 dig(EP)
Well 10 Pcons+RBS+AseI-5 dig(EP)
Well 11 Pcons+RBS+XhoI-1 dig(EP)
Well 12 Pcons+RBS+SacI-1 dig(EP)
Well 13 Pcons+RBS+KpnI-7+RBS+BFP+Ter dig(EP)
Well 14 SalI Kr dig(EP) 9/21
Well 15 SalI Kr dig(EP) 9/19
DNA sequencing of
KpnI-1 Cr dig(EP)
NdeI-1 Cr dig(EP)
XhoI-1 Cr dig(EP)
SacI-1 Cr dig(EP)
AseI-1 Cr dig(EP)
Pcons+RBS+HindIII-1 dig(EP)
Pcons+RBS+KpnI-4 dig(EP)
Pcons+RBS+NheI-4 dig(EP)(fail)
Pcons+RBS+AseI-5 dig(EP)
Pcons+RBS+XhoI-1 dig(EP)
Pcons+RBS+SacI-1 dig(EP)
Pcons+RBS+KpnI-7+RBS+BFP+Ter dig(EP)
Transformation of
Pcons+RBS+HindIII+RBS+BFP+Ter
Pcons+RBS+NcoI+RBS+BFP+Ter
Pcons+RBS+SalI+RBS+BFP+Ter
Pcons+RBS+SacI+RBS+BFP+Ter
9/24
Cultivation of
Pcons+RBS+NheI+RBS+BFP+Ter Cr
Pcons+RBS+AseI+RBS+BFP+Ter Cr
Pcons+RBS+NcoI+RBS+BFP+Ter Cr
Pcons+RBS+NdeI+RBS+BFP+Ter Cr
NheI Cr
psb3C5 Cr
Cultivation of
Pcons+RBS+NheI+RBS+BFP+Ter Cr
Pcons+RBS+AseI+RBS+BFP+Ter Cr
Pcons+RBS+NcoI+RBS+BFP+Ter Cr
Pcons+RBS+NdeI+RBS+BFP+Ter Cr
NheI Cr
psb3C5 Cr
Electrophoresis of
Well 1 Marker
Well 2 SalI Kr dig(EP)
Well 3 Pcons+RBS+XhoI+RBS+BFP+Ter Cr dig(EP)
Well 4 Pcons+RBS+SacI Cr dig(EP)
Well 5 NcoI Cr dig(EP)
Digestion of
Pcons+RBS+XhoI+RBS+BFP+Ter Cr dig(EP)
Pcons+RBS+SacI-2 Cr dig(EP)
NcoI-8 Cr dig(EP)
SalI-3 Kr dig(EP)
Ligation of
HindIII+psb1C3
PCR of
SacI x8
SalI x5
HindIII x2
NheI x8
Mini-prep and digestion of
Pcons+RBS+AseI+RBS+BFP+Ter
Pcons+RBS+NheI+RBS+BFP+Ter
Pcons+RBS+NdeI+RBS+BFP+Ter
Pcons+RBS+NcoI+RBS+BFP+Ter
psb3C5
NheI
9/25
Electrophoresis and DNA sequencing of
Well 1 Marker
Well 2 NheI (fail)
Well 3 Pcons+RBS+AseI+RBS+BFP+Ter
Well 4 Pcons+RBS+NcoI+RBS+BFP+Ter
Well 5 Pcons+RBS+NdeI+RBS+BFP+Ter (fail)
Transformation of
HindIII
SalI
Pcons+RBS+SacI+RBS+BFP+Ter
Pcons+RBS+SalI+RBS+BFP+Ter
Pcons+RBS+HindIII+RBS+BFP+Ter
PCR of
NcoI x5(9.10.11.12.13)
Pcons+RBS+SacI x5(7.8.9.10.11)
Cultivation of
NheI
Pcons+RBS+SacI-2(9/22)
Pcons+RBS+NcoI-8(9/22)
Pcons+RBS+NheI-4
Pcons+RBS+NdeI+RBS+BFP+Ter x2
Pcons+RBS+NheI+RBS+BFP+Ter x2
9/26
Mini-prep and digestion(EP) of
NcoI
NheI
Pcons+RBS+SacI-2
Pcons+RBS+NheI-4
Pcons+RBS+NdeI-1+RBS+BFP+Ter
Pcons+RBS+NdeI-2+RBS+BFP+Ter
Electrophoresis of
Well 1 Marker
Well 2 Pcons+RBS+NdeI-1+RBS+BFP+Ter
Well 3 Pcons+RBS+NdeI-2+RBS+BFP+Ter
Well 4 NcoI
Well 5 NheI
Well 6 Pcons+RBS+NheI-4
Well 7 Pcons+RBS+SacI-2
Cultivation of
psb3C5 x2
HindIII-3
HindIII-4
SalI-3
SalI-4
Pcons+RBS+HindIII-3+RBS+BFP+Ter
Pcons+RBS+HindIII-4+RBS+BFP+Ter
Pcons+RBS+SalI-3+RBS+BFP+Ter
Pcons+RBS+SalI-4+RBS+BFP+Ter
Pcons+RBS+SacI-3+RBS+BFP+Ter
Pcons+RBS+SacI-4+RBS+BFP+Ter
Pcons+RBS+NcoI-3+RBS+BFP+Ter
Pcons+RBS+NcoI-4+RBS+BFP+Ter
9/28
Electrophoresis of
Well 1 HindIII-3
Well 2 HindIII-4
Well 3 SalI-3
Well 4 SalI-4
Well 5 Pcons+RBS+SacI-3+RBS+BFP+Ter
Well 6 Marker
Well 7 Pcons+RBS+NcoI-3+RBS+BFP+Ter
Well 8 Pcons+RBS+NcoI-4+RBS+BFP+Ter
Well 9 Pcons+RBS+SalI-3+RBS+BFP+Ter
Well 10 Pcons+RBS+SalI-4+RBS+BFP+Ter
Well 11 psb3C5
9/29
Electrophoresis of
Well 1 Marker
Well 2 Pcons+RBS+HindIII+RBS+BFP+Ter
Well 3 Pcons+RBS+HindIII+RBS+BFP+Ter
Well 4 Pcons+RBS+HindIII+RBS+BFP+Ter
Well 5 Pcons+RBS+HindIII+RBS+BFP+Ter
Well 6 Pcons+RBS+HindIII+RBS+BFP+Ter
Well 7 Pcons+RBS+HindIII+RBS+BFP+Ter
Well 8 Pcons+RBS+NcoI+RBS+BFP+Ter
Well 9 Pcons+RBS+NcoI+RBS+BFP+Ter
Well 10 Pcons+RBS+NcoI+RBS+BFP+Ter
Well 11 Pcons+RBS+NcoI+RBS+BFP+Ter
Well 12 Pcons+RBS+NcoI+RBS+BFP+Ter
Well 13 Pcons+RBS+NcoI+RBS+BFP+Ter
Well 14 Pcons+RBS+SalI+RBS+BFP+Ter
Well 15 Pcons+RBS+SalI+RBS+BFP+Ter
Well 16 Pcons+RBS+SalI+RBS+BFP+Ter
Well 17 Pcons+RBS+SalI+RBS+BFP+Ter
Well 18 Pcons+RBS+SalI+RBS+BFP+Ter
Well 19 Pcons+RBS+SalI+RBS+BFP+Ter
Well 20 psb3C5 dig
Well 21 Marker
Ligation of
Pcons+RBS+KpnI + pbs3C5
Pcons+RBS+SacI + pbs3C5
Pcons+RBS+AseI + pbs3C5
Pcons+RBS+HindIII + pbs3C5
Pcons+RBS+NheI + pbs3C5
Pcons+RBS+SalI + pbs3C5
Pcons+RBS+NcoI + pbs3C5
Pcons+RBS+NdeI + pbs3C5
Pcons+RBS+XhoI+RBS+BFP+Ter + pbs1C3
Pcons+RBS+NdeI+RBS+BFP+Ter + pbs1C3
Pcons+RBS+SacI + pbs1C3
Pcons+RBS+NcoI + pbs1C3
DNA sequencing of
SalI-4
HindIII-4
Pcons+RBS+SalI-3+RBS+BFP+Ter
Pcons+RBS+SalI-2
Pcons+RBS+NheI-4
NheI(9/26)
Pcons+RBS+HindIII+RBS+BFP+Ter(9/30)
Pcons+RBS+SalI+RBS+BFP+Ter(9/30)
9/30
PCR and Electrophoresis of
Pcons+RBS+NdeI+RBS+BFP+Ter
NcoI
Pcons+RBS+NheI+RBS+BFP+Ter
Pcons+RBS+XhoI+RBS+BFP+Ter
Pcons+RBS+SacI+RBS+BFP+Ter
Cultivation of
Pcons+RBS+NheI-11+RBS+BFP+Ter
Pcons+RBS+NheI-13+RBS+BFP+Ter
Pcons+RBS+NdeI-3+RBS+BFP+Ter
Pcons+RBS+NdeI-10+RBS+BFP+Ter
Pcons+RBS+XhoI-3+RBS+BFP+Ter
Pcons+RBS+XhoI-5+RBS+BFP+Ter
Pcons+RBS+SacI-2+RBS+BFP+Ter
Pcons+RBS+SacI-11+RBS+BFP+Ter
NcoI-3
NcoI-12
October 2014
- Sun.
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- Wed.
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- Sat.
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10/3
Cultivation of
[Pcons+RBS+NdeI]Cr
[Pcons+RBS+NcoI]Cr
[KpnI-1]Cr
[NdeI-1]Cr
[XhoI-1]Cr
[SacI-1]Cr
[AseI-1]Cr
[Pcons+RBS+HindIII-1]Cr
[Pcons+RBS+KpnI-4]Cr
[Pcons+RBS+AseI-5]Cr
[Pcons+RBS+XhoI-1]Cr
[Pcons+RBS+SacI-1]Cr
[Pcons+RBS+KpnI-1+J61048]Cr
[SalI-4]Cr
[Pcons+RBS+SalI-3+J61048]Cr
[HindIII-4]Cr
[Pcons+RBS+AseI+J61048]Cr
[NheI]
[NcoI]
Electrophoresis of
[NdeI](EP)
[NcoI](EP)
[SacI](EP)
Transformation of
[Pcons+RBS+NdeI]Cr
[Pcons+RBS+SacI]Cr
DNA Sequencing of
[Pcons+RBS+NdeI+J61048-1]
[Pcons+RBS+HindIII+J61048-1]
[Pcons+RBS+SalI+J61048-4]
[Pcons+RBS+SacI+J61048-1]
[Pcons+RBS+SacI+J61048-1](fail)
10/4
Digestion of
[Pcons+RBS+XhoI+J61048-2, -4, -5]
[Pcons+RBS+SacI+J61048-10, -11]
[Pcons+RBS+NheI+J61048-20]
[Pcons+RBS+NheI]Cr
[Pcons+RBS+NcoI]Cr
Cultivation of
[Pcons+RBS+NheI-1, -2, -4, -6, -10, -12]
[Pcons+RBS+SalI-1, -3, -6, -7, -11, -12]
Cultivation of
[Pcons+RBS+NcoI+J61048-1~8]
PCR of
[Pcons+RBS+NheI-1, -2, -4, -6, -10, -12]
[Pcons+RBS+SacI-1, -3, -6, -7, -11, -12]
[Pcons+RBS+NcoI+J61048-1~8]
Ligation of
[Pcons+RBS+NheI]Kr
[Pcons+RBS+NcoI]Kr
Electrophoresis of
[XhoI-2, -4, -5](dig)
[SacI-10, -11](dig)
[NheI-20](dig)
[Pcons+RBS+NdeI](dig)
[Pcons+RBS+NcoI](dig)
[PCR NcoI-1~8]
Mini-prep of
[Pcons+RBS+NheI-6]
[Pcons+RBS+SalI-1, -7, -11]
Digestion of
[Pcons+RBS+NheI-6](EP)
[Pcons+RBS+SalI-1, -7, -11](EP)
[Pcons+RBS+NcoI]
[Pcons+RBS+Ndel]
PCR of and cultivation of
[Pcons+RBS+NcoI+J61048]
Mini-prep of
[Pcons+RBS+NcoI+J61048-7]Cr
Electrophoresis of
[Pcons+RBS+KphI+J61048-1]Cr
[Pcons+RBS+SalI+J61048-3]Cr
[Pcons+RBS+AseI+J61048 ]Cr
[Pcons+RBS+NdeI+J61048-1]
[Pcons+RBS+HindIII+J61048-1]
[Pcons+RBS+SacI+J61048 -1]
[Pcons+RBS+NcoI+J61048](dig)
[SacI-11]
DNA Sequencing of
[Pcons+RBS+NdeI]-2 Cr
[Pcons+RBS+NcoI]-2 Cr
[Pcons+RBS+NheI]-6 Cr
[Pcons+RBS+SalI-1]Cr
[Pcons+RBS+SalI-11]Cr
[Pcons+RBS+HindIII-1]Cr
[NcoI-12]
[Pcons+RBS+KpnI-1+J61048]Cr
[Pcons+RBS+AseI+J61048]Cr
[Pcons+RBS+SalI-3+J61048]Cr
[Pcons+RBS+HindIII-1+J61048]Cr
[Pcons+RBS+XhoI-2+J61048]Cr
[Pcons+RBS+XhoI-5+J61048]Cr
[Pcons+RBS+NcoI-5+J61048]Cr
[Pcons+RBS+SacI-11-1+J61048]Cr
[Pcons+RBS+NdeI-1+J61048]Cr
[Pcons+RBS+NheI-20+J61048]Cr
[Pcons+RBS+AseI-7+J61048]Cr
[Pcons+RBS+HindIII-7+J61048]Ar
[Pcons+RBS+SacI-16+J61048]Ar
[Pcons+RBS+SalI-17+J61048]Ar
[Pcons+RBS+NdeI-2+J61048]Cr
[Pcons+RBS+NcoI-2+J61048]Cr
[Pcons+RBS+XhoI+J61048]Ar
[Pcons+RBS+KpnI-7+J61048]Ar
[Pcons+RBS+NheI-11+J61048]Ar
10/7
PCR of
[Pcons+RBS+NheI+J61048]
[Pcons+RBS+SalI in PSB3C5]
[Pcons+RBS+SacI in PSB3C5]
Cultivation of
[Pcons+RBS+NheI+J61048-1, -3, -4, -6, -8, -10, -15, -16]
Transformation of
[Pcons+RBS+XhoI-2 +J61048]
[Pcons+RBS+XhoI-5 +J61048-1]
[Pcons+RBS+SacI-11+J61048]
PCR of
[Pcons+RBS+ NdeI in PSB3C5]
[Pcons+RBS+KpnI in PSB3C5]
[Pcons+RBS+AseI in PSB3C5]
[Pcons+RBS+XhoI in PSB3C5]
[Pcons+RBS+NcoI in PSB3C5]
[Pcons+RBS+NheI in PSB3C5]
nine kinds of PBAN
DNA Sequencing of
[Pcons+RBS+NcoI-5+J61048]
10/8
DNA Sequencing of
[PBAN NcoI]
[Pcons+RBS+SalI -1]
[Pcons+RBS+SalI-11]
[Pcons+RBS+NheI-6]
[Pcons+RBS+xhol-2+RBS+BFP+TER]
[Pcons+RBS+SacI-11+RBS+BFP+TER]
[Pcons+RBS+ Nhel-20+RBS+BFP+TER]
Ligation of
Pcons+RBS+NcoI on psb3c5
Pcons+RBS+NcoI on psb3c5
10/9
ALL Nhel-9(10/7)x2
ALL SacI-11(10/7)x1
ALL xhol-2(10/7)x1
Electrophoresis of
Gel 1
Well 1 Marker
Well 2 Pcons+RBS+NheI-20+RBS+BFP+TER
Well 3 Pcons+RBS+Hind-1+RBS+BFP+TER
Well 4 Pcons+RBS+KpnI-1+RBS+BFP+TER
Well 5 Pcons+RBS+Ndel-1+RBS+BFP+TER
2 Well 6 Pcons+RBS+NcoI-5+RBS+BFP+TER
Well 7 Pcons+RBS+AseI ALL
Well 8 PBAN NcoI-12
Well 9 Pcons+RBS+NheI-6
Well 10 Pcons+RBS+Hind-1
Well 11 Pcons+RBS+NcoI-20
Well 12 Pcons+RBS+NcoI-2-2
Well 13 Pcons+RBS+Ndel-2-1
Well 14 Pcons+RBS+Nedl-2-2
Well 15 Pcons+RBS+SacI-1
Well 16 Pcons+RBS+SacI-11
Well 17 Marker
Gel 2
Well 1 marker
Well 2 Pcons+RBS+NheI-11
Well 3 Pcons+RBS+Hind3
Well 4 Pcons+RBS+KpnI-7
Well 5 Pcons+RBS+SacI-16
Well 6 Pcons+RBS+SalI-12
Well 7 Pcons+RBS+Xhol
Transformation of
Pcons+RBS+SacI-3 on psb3c5
Pcons+RBS+KpnI-1 on psb3c5
Pcons+RBS+NcoI-1 on psb3c5
Mini-prep of and sequencing of
ALL NheI-2 x1
ALL SacI-11 x1
ALL Xhol-2 X1
10/10
Transformation of
Pcons+RBS+Ndel on psb3c5
Pcons+RBS+NcoI on psb3c5
Electrophoresis of
Well 1 Marker
Well 2 Pcons+RBS+SacI-3 on psb3c5
Well 3 Pcons+RBS+KpnI-1 on psb3c5
Well 4 Pcons+RBS+NcoI-1 on psb3c5
10/12
PCR of and Electrophoresis of
Well 1 Pcons+RBS+NcoI+psb3c5
Well 2 Pcons+RBS+Ndel+psb3c5
None
None
