Team:NJAU China

From 2014.igem.org

(Difference between revisions)
 
(145 intermediate revisions not shown)
Line 1: Line 1:
<html lang="en">
<html lang="en">
<head>
<head>
-
<meta charset="utf-8">
+
    <meta charset="utf-8">
-
<meta http-equiv="X-UA-Compatible" content="IE=edge">
+
    <meta http-equiv="X-UA-Compatible" content="IE=edge">
-
<meta name="viewport" content="width=device-width, initial-scale=1">
+
    <meta name="viewport" content="width=device-width, initial-scale=1">
-
<meta name="description" content="">
+
    <meta name="description" content="">
-
<meta name="author" content="">
+
    <meta name="author" content="">
-
<link rel="icon" href="../../favicon.ico">
+
    <link rel="icon" href="../../favicon.ico">
-
<title>Carousel Template for Bootstrap</title>
+
    <title>Home</title>
-
<link rel="stylesheet" href="http://cdn.bootcss.com/bootstrap/3.2.0/css/bootstrap.min.css">
+
    <script src="https://2014.igem.org/Team:NJAU_China/jquery.min.js?action=raw"></script>
-
<script src="http://cdn.bootcss.com/jquery/1.11.1/jquery.min.js"></script>
+
    <script src="https://2014.igem.org/Team:NJAU_China/bootstrap.min.js?action=raw"></script>
-
<script src="http://cdn.bootcss.com/bootstrap/3.2.0/js/bootstrap.min.js"></script>
+
-
<!-- HTML5 shim and Respond.js IE8 support of HTML5 elements and media queries -->
+
    <link rel="stylesheet" type="text/css"
-
<!--[if lt IE 9]>
+
          href="https://2014.igem.org/Team:NJAU_China/bootstrap.min.css?action=raw&amp;ctype=text/css">
-
      <script src="https://oss.maxcdn.com/html5shiv/3.7.2/html5shiv.min.js"></script>
+
    <link rel="stylesheet" type="text/css"
-
      <script src="https://oss.maxcdn.com/respond/1.4.2/respond.min.js"></script>
+
          href="https://2014.igem.org/Team:NJAU_China/bootstrap-theme.min.css?action=raw&amp;ctype=text/css">
 +
    <!-- HTML5 shim and Respond.js IE8 support of HTML5 elements and media queries -->
 +
    <!--[if lt IE 9]>
 +
    <script src="https://oss.maxcdn.com/html5shiv/3.7.2/html5shiv.min.js"></script>
 +
    <script src="https://oss.maxcdn.com/respond/1.4.2/respond.min.js"></script>
     <![endif]-->
     <![endif]-->
-
<!-- Custom styles for this template -->
+
    <!-- Custom styles for this template -->
-
<link href="carousel.css" rel="stylesheet">
+
    <link href="carousel.css" rel="stylesheet">
-
<style type="text/css">
+
    <style type="text/css">
-
body {
+
        body {
-
padding-bottom: 40px;
+
            padding-bottom: 40px;
-
background: #333333;
+
            background:#a2dcf3;
-
color: #5a5a5a;
+
            color: #5a5a5a;
-
font-size: 12px;
+
            font-size: 14px;
 +
            font-family:Andalus;
 +
        }
 +
        #myheader{
 +
            width:1366px;
 +
    height:500px;
 +
    margin:0 auto;
 +
    position:relative;
 +
    font-family:Arial, Helvetica, sans-serif;
 +
            background:#a2dcf3 url("https://static.igem.org/mediawiki/2014/3/34/Header_bg.png");
 +
        }
 +
        #contentSub, #footer-box, #catlinks, #search-controls, #p-logo, .printfooter, .visualClear {
 +
            display: none;
 +
        }
 +
 
 +
        /*-- hides default wiki settings --*/
 +
        .firstHeading {
 +
            width: 975px;
 +
            margin: 0px auto;
 +
            padding-top: 100px;
 +
            margin-bottom: 20px;
 +
            font-family: Georgia, Times, "Andalus", serif;
 +
        }
 +
 
 +
        h1, h2, h3, h4, h5 {font-family: Georgia, Times, "Andalus", serif;}
 +
        #top-section {
 +
            /*-- styling for default menu bar (edit, page, history, etc.) --*/ background-color: #383838;border: 0 none;height: 14px;z-index: 100;top: 0;position: fixed; width: 975px;left: 50%;margin-left: -487px;}
 +
#top-section-bar {
 +
            /*-- styling full width bar which hides behind default menu bar (edit, page, history, etc.) --*/
 +
  background-color: #383838;
 +
  height: 14px;
 +
  display: block;
 +
  z-index: 10;position: fixed; width: 100%; top: 0;}
 +
  body { background-color: #fff;
 +
  }
 +
#globalWrapper, #content {
 +
  /*-- changes default wiki settings --*/
 +
  width: 100%;
 +
  height: 100%;
 +
  border: 0px;
 +
  background-color: transparent;
 +
  margin: 0px;
 +
  padding: 0px;
 +
  }
 +
 
 +
  html, body, .wrapper {
 +
  /*-- changes default wiki settings --*/
 +
  width: 100%;
 +
  height: 100%;
 +
  background-color: transparent;
 +
  } #container {
 +
  /*-- creates container for all content on page --*/
 +
  font-family: Arial, Helvetica, sans-serif;
 +
            font-weight: normal;
 +
            font-size: 14px;
 +
            color: #414141;
 +
            width: 960px;
 +
            margin-left: auto;
 +
            margin-right: auto;
 +
            background-color: #transparent;
 +
            margin-top: 0px;}
 +
.container {
 +
            font-size: 14px;
 +
            width:700px;
 +
            float:right;}
 +
.buttontext { color: #FFF}
 +
#my_h1{position:absolute;top:70px;left:170px;border:none;}
 +
#my_h1 a{display:block;width:190px;height:130px;text-indent:-9999px;}
 +
#my_h2{position:absolute;top:120px;left:370px;border:none;}
 +
#my_h2 a{display:block;width:390px;height:230px;text-indent:-9999px;}
 +
#my_nav{position:absolute;top:402px;left:200px;width:990px;height:50px;}
 +
#my_nav ul{width:100%;margin:0;padding:0;list-style:none;font-size:24px;}
 +
#my_nav ul li{display::inline-block;padding:9px 30px;float:left;list-style:none;position:relative;}
 +
#my_nav ul li:hover{background:#e3f7ff;}#my_nav ul li a{color:#00586b;text-decoration:none;}#project{display:none;position:absolute;z-index:100;top:47px;left:0px;background:#CF6;width:100px;}#project dd{width:135px;font-size:16px;
 +
background:#c9efff;height:40px;margin:0;}#project dd a{width:100%;height:40px;display:block;text-decoration:none;text-align:center;line-height:40px;}#project dd a:hover{background:#a1dbf0;}#achievement{display:none;position:absolute;top:47px;z-index:100;left:0px;background:#CF6;width:100px;}#achievement dd{width:198px;font-size:16px;background:#c9efff;height:40px;margin:0;}#achievement dd a{width:100%;height:40px;display:block;text-decoration:none;text-align:center;line-height:40px;}#achievement dd a:hover{background:#a1dbf0;}#prctice{display:none;position:absolute;top:47px;z-index:100;left:0px;background:#CF6;width:100px;}#prctice dd{width:300px;font-size:16px;background:#c9efff;height:40px;margin:0;}#prctice dd a{width:100%;height:40px;display:block;text-decoration:none;text-align:center;line-height:40px;}#prctice dd a:hover{background:#a1dbf0;}#ackonwledgement{display:none;position:absolute;top:47px;z-index:100;left:0px;background:#CF6;width:100px;}#ackonwledgement dd{width:257px;font-size:16px;background:#c9efff;height:40px;margin:0;}#ackonwledgement dd a{width:100%;height:40px;display:block;text-decoration:none;text-align:center;line-height:40px;}#ackonwledgement dd a:hover{background:#a1dbf0;}
 +
#my_content{
 +
width:1030px;
 +
margin:0px auto;}
 +
#my_abstract{
 +
width:300px;
 +
margin:0 0 0 25px;
 +
        float:left;
 +
}
 +
#my_abstract h2{
 +
height:50px;
 +
line-height:50px;
 +
text-indent:50px;
 +
border:none;
 +
background:#999;
 +
margin:0 0 20px 0;
 +
padding:0;
}
}
-
#contentSub, #footer-box, #catlinks, #search-controls, #p-logo, .printfooter, .visualClear {
+
#my_abstract p{
-
display: none;
+
text-indent:30px;
-
} /*-- hides default wiki settings --*/
+
height:240px;
-
.firstHeading {
+
overflow-y:scroll;
-
width: 975px;
+
-
margin: 0px auto;
+
-
padding-top: 100px;
+
-
margin-bottom: 20px;
+
-
font-family: Georgia, Times, "Times New Roman", serif;
+
}
}
-
h1, h2, h3, h4, h5 {
+
 
-
font-family: Georgia, Times, "Times New Roman", serif;
+
 
 +
 
 +
 
 +
#my_contents{
 +
width:1030px;
 +
margin:30px auto;
 +
height:800px;
}
}
-
#top-section { /*-- styling for default menu bar (edit, page, history, etc.) --*/
+
#my_con{
-
background-color: #383838;
+
width:1000px;
-
border: 0 none;
+
height:796px;
-
height: 14px;
+
float:right;
-
z-index: 100;
+
position:relative;
-
top: 0;
+
border:2px solid #999;
-
position: fixed;
+
        margin:20px 10px 0 0;
-
width: 975px;
+
-
left: 50%;
+
-
margin-left: -487px;
+
}
}
-
#top-section-bar { /*-- styling full width bar which hides behind default menu bar (edit, page, history, etc.) --*/
+
#stage_1{
-
background-color: #383838;
+
width:20px;
-
height: 14px;
+
height:20px;
-
display: block;
+
position:absolute;
-
z-index: 10;
+
top:40px;
-
position: fixed;
+
left:40px;
-
width: 100%;
+
border-left:2px solid #333;
-
top: 0;
+
border-top:2px solid #333;
}
}
-
#menubar a:link, #menubar a:active, #menubar a:visited, #menubar a:hover, #menubar:hover { /*-- styling for default menu bar links (edit, page, history, etc.) --*/
+
#stage_2{
-
color: #727272;
+
width:20px;
-
text-decoration: none;
+
height:20px;
-
background-color: transparent;
+
position:absolute;
 +
top:40px;
 +
left:940px;
 +
border-right:2px solid #333;
 +
border-top:2px solid #333;
}
}
-
body {
+
#stage_3{
-
background-color: #fff;
+
width:20px;
 +
height:20px;
 +
position:absolute;
 +
top:740px;
 +
left:40px;
 +
border-bottom:2px solid #333;
 +
border-left:2px solid #333;
}
}
-
#globalWrapper, #content { /*-- changes default wiki settings --*/
+
#stage_4{
-
width: 100%;
+
width:20px;
-
height: 100%;
+
height:20px;
-
border: 0px;
+
position:absolute;
-
background-color: transparent;
+
top:740px;
-
margin: 0px;
+
left:940px;
-
padding: 0px;
+
border-right:2px solid #333;
 +
border-bottom:2px solid #333;
}
}
-
html, body, .wrapper { /*-- changes default wiki settings --*/
+
#real_con{
-
width: 100%;
+
position:absolute;
-
height: 100%;
+
top:60px;
-
background-color: transparent;
+
left:60px;
 +
width:880px;
 +
height:680px;
}
}
-
#container { /*-- creates container for all content on page --*/
+
#real_con ul{
-
font-family: Arial, Helvetica, sans-serif;
+
list-style:none;
-
font-weight: normal;
+
width:100%;
-
font-size: 14px;
+
height:100%;
-
color: #414141;
+
margin:0;
-
width: 960px;
+
padding:0;
-
margin-left: auto;
+
-
margin-right: auto;
+
-
background-color: #transparent;
+
-
margin-top: 0px;
+
}
}
-
.sidemenu, .sidemenu li {
+
#real_con ul li{
-
list-style-type: none;
+
width:400px;
-
list-style-image: none;
+
height:300px;
-
font-family: verdana;
+
float:left;
-
text-decoration: none;
+
background:#0F0;
-
color:#000;
+
margin:26px 0 0 26px;}
-
font-size: 14px;
+
#real_con ul li#one_1{
-
}
+
background:url("https://static.igem.org/mediawiki/2014/b/b4/Project_li_bg.png") no-repeat;}
-
.sidemenu li {
+
#real_con ul li#two_2{
-
display: block;
+
background:url("https://static.igem.org/mediawiki/2014/1/17/Model_li_bg.png") no-repeat;}
-
}
+
#real_con ul li#three_3{
-
.sidemenu a {
+
background:url("https://static.igem.org/mediawiki/2014/6/6e/Weblab_li_bg.png") no-repeat;}
 +
#real_con ul li#four_4{
 +
background:url("https://static.igem.org/mediawiki/2014/f/f0/Practice_li_bg.png") no-repeat;}
 +
#real_con ul li a{
text-decoration:none;
text-decoration:none;
-
color: #000;
+
display:block;
-
display: block;
+
width:100%;
-
width: 180px;
+
height:100%;
-
height: 100%;
+
-
padding: 3px 5px;
+
-
transition: .25s ease-in-out;
+
-
-moz-transition: .25s ease-in-out;
+
-
-webkit-transition: .25s ease-in-out;
+
}
}
-
.sidemenu a.greyout {
+
#real_con ul li a#one:hover{
-
color: #909090;
+
background:url("https://static.igem.org/mediawiki/2014/6/68/Project_a_bg.png") no-repeat;}
 +
 
 +
#real_con ul li a#two:hover{
 +
background:url("https://static.igem.org/mediawiki/2014/d/dc/Model_a_bg.png") no-repeat;}
 +
#real_con ul li a#three:hover{
 +
background:url("https://static.igem.org/mediawiki/2014/6/60/Weblab_a_bg.png") no-repeat;}
 +
#real_con ul li a#four:hover{
 +
background:url("https://static.igem.org/mediawiki/2014/7/7f/Practice_a_bg.png") no-repeat;}
 +
 
 +
 
 +
#thanks{
 +
height:177px;
 +
width:1030px;
 +
padding:30px 0 0 0;
 +
        clear:both;
 +
margin:30px auto 0;}
 +
 
 +
 
 +
    </style>
 +
    <style>
 +
        #contentSub, #footer-box, #catlinks, #search-controls, #p-logo, .printfooter, .firstHeading, .visualClear {
 +
            display: none;
 +
        }
 +
 
 +
        /*-- hides default wiki settings --*/
 +
    </style>
 +
<script type="text/javascript">
 +
window.onload = function(){
 +
xialashow('project_li','project');
 +
xialahidden('project_li','project');
 +
xialashow('achievement_li','achievement');
 +
xialahidden('achievement_li','achievement');
 +
xialashow('prctice_li','prctice');
 +
xialahidden('prctice_li','prctice');
 +
xialashow('ackonwledgement_li','ackonwledgement');
 +
xialahidden('ackonwledgement_li','ackonwledgement');
}
}
-
.sidemenu a:hover {
+
function xialashow(li_id,dl_id){
-
color:#666;
+
var li_id = document.getElementById(li_id);
-
background-color: #d9f5aa;
+
var dl_id = document.getElementById(dl_id);
 +
li_id.onmouseover = function(){
 +
dl_id.style.display = 'block';
 +
}
}
}
-
.sub-sidemenu {
+
function xialahidden(li_id,dl_id){
-
font-size: 10px;
+
var li_id = document.getElementById(li_id);
-
margin-bottom: 20px;
+
var dl_id = document.getElementById(dl_id);
-
color: #666;
+
li_id.onmouseout = function(){
-
display: none;
+
dl_id.style.display = 'none';
 +
}
}
}
-
.sub-sidemenu a {
+
</script>
-
color: #666;
+
 
-
}
+
-
div.underconst {
+
-
padding:10px;
+
-
border-radius:5px;
+
-
background-color:pink;
+
-
text-align:center;
+
-
}
+
-
a.anchor {
+
-
display: block;
+
-
position: relative;
+
-
top: -80px;
+
-
visibility: hidden;
+
-
}
+
-
.contentpara {
+
-
margin-bottom: 30px;
+
-
}
+
-
span.email {
+
-
font-family: monospace;
+
-
font-weight: normal;
+
-
}
+
-
fieldset {
+
-
border: 1px solid #337f53;
+
-
}
+
-
.greyout {
+
-
color: #A0A0A0;
+
-
}
+
-
.highlightme {
+
-
background-color: #FFFF00;
+
-
}
+
-
#alertContainer {
+
-
border: 2px solid red;
+
-
margin-bottom: 10px;
+
-
width: 936px;
+
-
}
+
-
#annContainer {
+
-
margin-left: 8px;
+
-
}
+
-
#newsContainer {
+
-
border: 1px solid #ccc;
+
-
}
+
-
.newsTitle {
+
-
display: block;
+
-
color: #414141;
+
-
font-size: 25px;
+
-
font-family: Georgia, Times, "Times New Roman", serif;
+
-
padding: 10px 15px 5px 10px;
+
-
border-bottom: 1px solid #ccc;
+
-
margin-bottom: 0px;
+
-
}
+
-
.newsItem {
+
-
border-bottom: 1px solid #ccc;
+
-
display: block;
+
-
padding: 5px 15px 0px 10px;
+
-
margin-bottom: 0px;
+
-
}
+
-
.newsItem h3 {
+
-
width: auto;
+
-
display: inline;
+
-
font-size: 14px;
+
-
font-family: Arial, Helvetica, sans-serif;
+
-
padding: 0px;
+
-
margin: 0px;
+
-
}
+
-
.newsItem img {
+
-
float: right;
+
-
clear: right;
+
-
width: 80px;
+
-
padding-left: 15px;
+
-
display: inline-block;
+
-
}
+
-
.newsItem .newsDate {
+
-
font-style: italic;
+
-
font-size: 14px;
+
-
display: inline-block;
+
-
color: #999;
+
-
float: right;
+
-
padding: 0px 0px 10px 15px;
+
-
}
+
-
.newsItem p, .newsItem ul, .newsItem li {
+
-
padding: 0px;
+
-
margin-left: 20px;
+
-
font-size: 12px;
+
-
line-height: 1.2;
+
-
}
+
-
.newsItem p {
+
-
margin-top: 10px;
+
-
}
+
-
.annItem {
+
-
border: 2px solid #49b677;
+
-
display: block;
+
-
padding: 0px 15px 10px 10px;
+
-
margin-bottom: 18px;
+
-
position: relative;
+
-
height: 160px;
+
-
vertical-align: middle;
+
-
}
+
-
.annItem .annCentered {
+
-
display: table-cell;
+
-
vertical-align: middle;
+
-
height: 160px;
+
-
}
+
-
.annCentered h3 {
+
-
padding: 0px;
+
-
display: block;
+
-
margin-left: auto;
+
-
margin-right: auto;
+
-
text-align: center;
+
-
}
+
-
.annItem ul, .annItem li {
+
-
padding: 0px;
+
-
margin: 0px;
+
-
}
+
-
.annItem li {
+
-
list-style: none;
+
-
margin: 5px
+
-
}
+
-
.container {
+
-
font-size:14px
+
-
}
+
-
.buttontext {
+
-
color:#FFF
+
-
}
+
-
</style>
+
-
<style>
+
-
#contentSub, #footer-box, #catlinks, #search-controls, #p-logo, .printfooter, .firstHeading,.visualClear {display: none;} /*-- hides default wiki settings --*/
+
-
</style>
+
</head>
</head>
 +
<!-- NAVBAR
<!-- NAVBAR
================================================== -->
================================================== -->
<body>
<body>
-
<div class="navbar-wrapper">
+
<div id="myheader">
-
  <div class="container">
+
<h1 id="my_h1"><a href="https://2014.igem.org">https://2014.igem.org</a></h1>
-
    <div class="navbar navbar-fixed-top navbar-inverse" role="navigation">
+
<h2 id="my_h2"><a href="https://igem.org/Team.cgi?id=1555">https://igem.org/Team.cgi?id=1555</a></h2>
-
      <div class="container">
+
    <div id="my_nav">
-
         <div class="navbar-header">
+
         <ul>
-
          <button type="button" class="navbar-toggle collapsed" data-toggle="collapse" data-target=".navbar-collapse"> <span class="sr-only">Toggle navigation</span> <span class="icon-bar"></span> <span class="icon-bar"></span> <span class="icon-bar"></span> </button>
+
          <li><a href="https://2014.igem.org/Team:NJAU_China">Home</a></li>
-
          <a class="navbar-brand" href="#">Project name</a> </div>
+
          <li id="project_li"><a href="#">Project</a>
-
        <div class="collapse navbar-collapse">
+
                <dl id="project">
-
          <ul class="nav navbar-nav">
+
                    <dd><a href="https://2014.igem.org/Team:NJAU_China/Background">Background</a></dd>
-
            <li class="active"><a href="https://2014.igem.org/Team:NJAU_China" class="unselect">Home</a></li>
+
                    <dd><a href="https://2014.igem.org/Team:NJAU_China/System Design">System Design</a></dd>
-
            <li><a href="https://2014.igem.org/Team:NJAU_China/Team"  class="unselect">Team</a></li>
+
                    <dd><a href="https://2014.igem.org/Team:NJAU_China/Safety">Safety</a></dd>
-
            <li><a href="https://igem.org/Team.cgi?year=2014" class="unselect">Official Team Profile </a></li>
+
                </dl>
-
            <li><a href="https://2014.igem.org/Team:NJAU_China/Project">Project</a></li>
+
          </li>
-
            <li><a href="https://2014.igem.org/Team:NJAU_China/Parts" class="unselect">Parts</a></li>
+
          <li id="achievement_li"><a href="#">Achievement</a>
-
            <li><a href="https://2014.igem.org/Team:NJAU_China/Modeling" class="unselect">Modeling</a></li>
+
                <dl id="achievement">
-
            <li><a href="https://2014.igem.org/Team:NJAU_China/Notebook" class="unselect">Notebook</a></li>
+
                    <dd><a href="https://2014.igem.org/Team:NJAU_China/Wet Labs">Wet Lab</a></dd>
-
            <li><a href="https://2014.igem.org/Team:NJAU_China/Safety" class="unselect">Safety</a></li>
+
                    <dd><a href="https://2014.igem.org/Team:NJAU_China/Modeling">Model</a></dd>
-
            <li><a href="https://2014.igem.org/Team:NJAU_China/Attributions" class="unselect">Attributions</a></li>
+
                    <dd><a href="https://2014.igem.org/Team:NJAU_China/Protocol/Notebooks">Protocol/Notebooks</a></dd>
-
          </ul>
+
                    <dd><a href="https://2014.igem.org/Team:NJAU_China/Biobricks">Biobricks</a></dd>
-
        </div>
+
                </dl>
-
        <!-- /.nav-collapse -->  
+
          </li>
-
      </div>
+
          <li id="prctice_li"><a href="#">Practice</a>
-
      <!-- /.container -->  
+
                <dl id="prctice">
 +
                    <dd><a href="https://2014.igem.org/Team:NJAU_China/Exhibition and Workshops">Exhibitions and Workshops</a></dd>
 +
                    <dd><a href="https://2014.igem.org/Team:NJAU_China/Questionnaire Survey">Questionnaire Survey</a></dd>
 +
                    <dd><a href="https://2014.igem.org/Team:NJAU_China/Collaboration">Collaboration</a></dd>
 +
                </dl>
 +
          </li>
 +
          <li><a href="https://2014.igem.org/Team:NJAU_China/Team">Team</a></li>
 +
          <li id="ackonwledgement_li"><a href="#">Acknowledgement</a>
 +
                <dl id="ackonwledgement">
 +
                      <dd><a href="https://2014.igem.org/Team:NJAU_China/Inside Campus">Inside Campus&Arrtibutions</a></dd>
 +
                    <dd><a href="https://2014.igem.org/Team:NJAU_China/Outside Campus">Outside Campus</a></dd>
 +
                </dl>
 +
          </li>
 +
      </ul>
     </div>
     </div>
-
    <!-- /.navbar -->
 
-
   
 
-
  </div>
 
</div>
</div>
 +
 +
 +
<!---------导航结束------------>
 +
<!-- Carousel
<!-- Carousel
     ================================================== -->
     ================================================== -->
-
<div id="myCarousel" class="carousel slide" data-ride="carousel">  
+
 
-
  <!-- Indicators -->
+
<div id="my_content">
-
  <ol class="carousel-indicators">
+
<div id="my_abstract">
-
    <li data-target="#myCarousel" data-slide-to="0" class="active"></li>
+
    <h2>Abstract</h2>
-
    <li data-target="#myCarousel" data-slide-to="1"></li>
+
    <p>As an common pollutant source of industry, low concentration of copper ions can do harm to many creatures. Whereas, <i>Escherichia coli</i> has high tolerance for copper through specific systems. There are <i>cop</i>A promoter,<i>mar</i>O（operator of mar locus） and <i>cue</i>O promoter existing in <i>E.Coli</i>, all of which are directly or indirectly regulated by copper ions. The most sensitive and effective one (<i>cop</i>A)was chosen to trigger the sequential reactions. Glutathione is an intracellular redox-active tripeptide thiol which can effectively detoxify the metal ions by chelation. It can be synthesized by <i>gsh</i>F, an coding sequence derived from <i>Streptococcus thermophilus</i>, which can unify both necessary reactions in one platform.In addition, we also utilize sgRNA-dCas9 system to enhance the efficiency of chelate cupric and cuprous by repressing the expression of <i>cop</i>A. With all these design, an automatically and efficiently copper-preventing guard have been created successfully. </p>
-
    <li data-target="#myCarousel" data-slide-to="2"></li>
+
</div>
-
  </ol>
+
<div class="container">
-
  <div class="carousel-inner" role="listbox">
+
    <div id="myCarousel" class="carousel slide" data-ride="carousel">
-
    <div class="item active text-center"> <img src="https://static.igem.org/mediawiki/2014/d/d3/Njau_slide1.jpg" alt="First slide" class="text-center">
+
        <!-- Indicators -->
-
      <div class="container">
+
        <ol class="carousel-indicators">
-
        <div class="carousel-caption">
+
            <li data-target="#myCarousel" data-slide-to="0" class="active"></li>
-
          <h1>Example headline.</h1>
+
            <li data-target="#myCarousel" data-slide-to="1"></li>
-
          <p>Note: If you're viewing this page via a <code>file://</code> URL, the "next" and "previous" Glyphicon buttons on the left and right might not load/display properly due to web browser security rules.</p>
+
            <li data-target="#myCarousel" data-slide-to="2"></li>
-
          <p><a class="btn btn-lg btn-primary" style="color:#FFF" href="#" role="button">Sign up today</a></p>
+
        </ol>
 +
        <div class="carousel-inner" role="listbox">
 +
            <div class="item active text-center"><img src="https://static.igem.org/mediawiki/2014/d/d3/Njau_slide1.jpg"
 +
                                                      alt="First slide" class="text-center"></div>
 +
            <div class="item text-center"><img src="https://static.igem.org/mediawiki/2014/3/38/Face.jpg"
 +
                                              alt="Second slide"></div>
 +
            <div class="item text-center"><img src="https://static.igem.org/mediawiki/2014/9/94/Face2.jpg"
 +
                                              alt="Third slide"></div>
         </div>
         </div>
-
      </div>
+
        <a class="left carousel-control" href="#myCarousel" role="button" data-slide="prev"> <span class="sr-only">Previous</span>
-
    </div>
+
        </a> <a class="right carousel-control" href="#myCarousel" role="button" data-slide="next"> <span
-
    <div class="item text-center"> <img src="https://static.igem.org/mediawiki/2014/2/24/Njau_slide2.jpg" alt="Second slide">
+
            class="sr-only">Next</span> </a></div>
-
      <div class="container">
+
    <!-- /.carousel -->
-
         <div class="carousel-caption">
+
</div>
-
          <h1>Another example headline.</h1>
+
</div>
-
          <p>Cras justo odio, dapibus ac facilisis in, egestas eget quam. Donec id elit non mi porta gravida at eget metus. Nullam id dolor id nibh ultricies vehicula ut id elit.</p>
+
<div id="my_contents">
-
          <p><a class="btn btn-lg btn-primary" style="color:#FFF" href="#" role="button">Learn more</a></p>
+
<div id="my_con">
 +
    <div id="stage_1"></div>
 +
         <div id="stage_2"></div>
 +
        <div id="stage_3"></div>
 +
        <div id="stage_4"></div>
 +
        <div id="real_con">
 +
        <ul>
 +
            <li id="one_1"><a href="https://2014.igem.org/Team:NJAU_China/Background" id="one"></a></li>
 +
                <li id="two_2"><a href="https://2014.igem.org/Team:NJAU_China/Modeling" id="two"></a></li>
 +
                <li id="three_3"><a href="https://2014.igem.org/Team:NJAU_China/Wet_Labs" id="three"></a></li>
 +
                <li id="four_4"><a href="https://2014.igem.org/Team:NJAU_China/Questionnaire_Survey" id="four"></a></li>
 +
            </ul>
         </div>
         </div>
-
      </div>
 
     </div>
     </div>
-
    <div class="item text-center"> <img src="https://static.igem.org/mediawiki/2014/6/62/Njau_slide3.jpg" alt="Third slide">
+
</div>
-
      <div class="container">
+
-
        <div class="carousel-caption">
+
-
          <h1>One more for good measure.</h1>
+
-
          <p>Cras justo odio, dapibus ac facilisis in, egestas eget quam. Donec id elit non mi porta gravida at eget metus. Nullam id dolor id nibh ultricies vehicula ut id elit.</p>
+
-
          <p><a class="btn btn-lg btn-primary" style="color:#FFF" href="#" role="button">Browse gallery</a></p>
+
-
        </div>
+
-
      </div>
+
-
    </div>
+
-
  </div>
+
-
  <a class="left carousel-control" href="#myCarousel" role="button" data-slide="prev"> <span class="glyphicon glyphicon-chevron-left"></span> <span class="sr-only">Previous</span> </a> <a class="right carousel-control" href="#myCarousel" role="button" data-slide="next"> <span class="glyphicon glyphicon-chevron-right"></span> <span class="sr-only">Next</span> </a> </div>
+
-
<!-- /.carousel -->
+
-
<br/>
+
-
<!-- Marketing messaging and featurettes
+
-
    ================================================== -->
+
-
<!-- Wrap the rest of the page in another container to center all the content. -->
+
-
<div class="container marketing">  
+
<div id="thanks">
-
 
+
<img src="https://static.igem.org/mediawiki/2014/2/26/Njau_bg.png" alt="njau"><img src="https://static.igem.org/mediawiki/2014/a/ae/Shengke_bg.png" alt="shengke">
-
  <!-- Three columns of text below the carousel -->
+
-
  <div class="row">
+
-
    <div class="col-lg-4"> <img class="img-circle" src="https://static.igem.org/mediawiki/2014/c/c3/Avatar1.jpg" alt="Generic placeholder image" style="width: 140px; height: 140px;">
+
-
      <h2>Huiyu Li</h2>
+
-
      <p>I love cat</p>
+
-
      <p><a class="btn btn-primary" href="#" role="button">View details &raquo;</a></p>
+
-
    </div>
+
-
    <!-- /.col-lg-4 -->
+
-
    <div class="col-lg-4"> <img class="img-circle" src="https://static.igem.org/mediawiki/2014/e/e8/Avatar2.jpg" alt="Generic placeholder image" style="width: 140px; height: 140px;">
+
-
      <h2>Heng Xu</h2>
+
-
      <p>I love cat</p>
+
-
      <p><a class="buttontext btn btn-primary " style="color:#FFF" href="#" role="button">View details &raquo;</a></p>
+
-
    </div>
+
-
    <!-- /.col-lg-4 -->
+
-
    <div class="col-lg-4"> <img class="img-circle" src="https://static.igem.org/mediawiki/2014/4/46/Avatar3.jpg" alt="Generic placeholder image" style="width: 140px; height: 140px;">
+
-
      <h2>Zhenxiong Jiang</h2>
+
-
      <p>I love cat</p>
+
-
      <p><a class="btn btn-primary " style="color:#FFF" href="#" role="button">View details &raquo;</a></p>
+
-
    </div>
+
-
    <!-- /.col-lg-4 -->
+
-
  </div>
+
-
  <!-- /.row -->
+
-
 
+
-
  <!-- START THE FEATURETTES -->
+
-
 
+
-
  <hr class="featurette-divider">
+
-
  <div class="row featurette">
+
-
    <div class="col-md-7">
+
-
      <h2 class="featurette-heading">First featurette heading. <span class="text-muted">It'll blow your mind.</span></h2>
+
-
      <p class="lead">Donec ullamcorper nulla non metus auctor fringilla. Vestibulum id ligula porta felis euismod semper. Praesent commodo cursus magna, vel scelerisque nisl consectetur. Fusce dapibus, tellus ac cursus commodo.</p>
+
-
    </div>
+
-
    <div class="col-md-5"> <img class="featurette-image img-responsive" data-src="holder.js/500x500/auto" alt="Generic placeholder image"> </div>
+
-
  </div>
+
-
  <hr class="featurette-divider">
+
-
  <div class="row featurette">
+
-
    <div class="col-md-5"> <img class="featurette-image img-responsive" data-src="holder.js/500x500/auto" alt="Generic placeholder image"> </div>
+
-
    <div class="col-md-7">
+
-
      <h2 class="featurette-heading">Oh yeah, it's that good. <span class="text-muted">See for yourself.</span></h2>
+
-
      <p class="lead">Donec ullamcorper nulla non metus auctor fringilla. Vestibulum id ligula porta felis euismod semper. Praesent commodo cursus magna, vel scelerisque nisl consectetur. Fusce dapibus, tellus ac cursus commodo.</p>
+
-
    </div>
+
-
  </div>
+
-
  <hr class="featurette-divider">
+
-
  <div class="row featurette">
+
-
    <div class="col-md-7">
+
-
      <h2 class="featurette-heading">And lastly, this one. <span class="text-muted">Checkmate.</span></h2>
+
-
      <p class="lead">Donec ullamcorper nulla non metus auctor fringilla. Vestibulum id ligula porta felis euismod semper. Praesent commodo cursus magna, vel scelerisque nisl consectetur. Fusce dapibus, tellus ac cursus commodo.</p>
+
-
    </div>
+
-
    <div class="col-md-5"> <img class="featurette-image img-responsive" data-src="holder.js/500x500/auto" alt="Generic placeholder image"> </div>
+
-
  </div>
+
-
  <hr class="featurette-divider">
+
-
 
+
-
  <!-- /END THE FEATURETTES -->
+
-
 
+
-
  <!-- FOOTER -->
+
-
  <footer>
+
-
    <p class="pull-right"><a href="#">Back to top</a></p>
+
-
    <p>&copy; 2014 Company, Inc. &middot; <a href="#">Privacy</a> &middot; <a href="#">Terms</a></p>
+
-
  </footer>
+
</div>
</div>
-
<!-- /.container -->
 
-
 
-
<!-- Bootstrap core JavaScript
 
-
    ================================================== -->
 
-
<!-- Placed at the end of the document so the pages load faster -->
 
-
<!-- ?? Bootstrap ???? CSS ??? -->
 
-
<link rel="stylesheet" href="http://cdn.bootcss.com/bootstrap/3.2.0/css/bootstrap.min.css">
 
-
 
-
<!-- jQuery??????????bootstrap.min.js ?????? -->
 
-
<script src="http://cdn.bootcss.com/jquery/1.11.1/jquery.min.js"></script>
 
-
 
-
<!-- ???��? Bootstrap ???? JavaScript ??? -->
 
-
<script src="http://cdn.bootcss.com/bootstrap/3.2.0/js/bootstrap.min.js"></script>
 
</body>
</body>
</html>
</html>

Latest revision as of 17:03, 19 November 2014

Home

Abstract

As an common pollutant source of industry, low concentration of copper ions can do harm to many creatures. Whereas, Escherichia coli has high tolerance for copper through specific systems. There are copA promoter,marO（operator of mar locus） and cueO promoter existing in E.Coli, all of which are directly or indirectly regulated by copper ions. The most sensitive and effective one (copA)was chosen to trigger the sequential reactions. Glutathione is an intracellular redox-active tripeptide thiol which can effectively detoxify the metal ions by chelation. It can be synthesized by gshF, an coding sequence derived from Streptococcus thermophilus, which can unify both necessary reactions in one platform.In addition, we also utilize sgRNA-dCas9 system to enhance the efficiency of chelate cupric and cuprous by repressing the expression of copA. With all these design, an automatically and efficiently copper-preventing guard have been created successfully.

njaushengke

Retrieved from "http://2014.igem.org/Team:NJAU_China"