Team:NJAU China

From 2014.igem.org

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<!-- NAVBAR
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================================================== -->
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<body>
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<div id="myheader">
 +
<h1 id="my_h1"><a href="https://2014.igem.org">https://2014.igem.org</a></h1>
 +
<h2 id="my_h2"><a href="https://igem.org/Team.cgi?id=1555">https://igem.org/Team.cgi?id=1555</a></h2>
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    <div id="my_nav">
 +
        <ul>
 +
          <li><a href="https://2014.igem.org/Team:NJAU_China">Home</a></li>
 +
          <li id="project_li"><a href="#">Project</a>
 +
                <dl id="project">
 +
                    <dd><a href="https://2014.igem.org/Team:NJAU_China/Background">Background</a></dd>
 +
                    <dd><a href="https://2014.igem.org/Team:NJAU_China/System Design">System Design</a></dd>
 +
                    <dd><a href="https://2014.igem.org/Team:NJAU_China/Safety">Safety</a></dd>
 +
                </dl>
 +
          </li>
 +
          <li id="achievement_li"><a href="#">Achievement</a>
 +
                <dl id="achievement">
 +
                    <dd><a href="https://2014.igem.org/Team:NJAU_China/Wet Labs">Wet Lab</a></dd>
 +
                    <dd><a href="https://2014.igem.org/Team:NJAU_China/Modeling">Model</a></dd>
 +
                    <dd><a href="https://2014.igem.org/Team:NJAU_China/Protocol/Notebooks">Protocol/Notebooks</a></dd>
 +
                    <dd><a href="https://2014.igem.org/Team:NJAU_China/Biobricks">Biobricks</a></dd>
 +
                </dl>
 +
          </li>
 +
          <li id="prctice_li"><a href="#">Practice</a>
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 +
                    <dd><a href="https://2014.igem.org/Team:NJAU_China/Exhibition and Workshops">Exhibitions and Workshops</a></dd>
 +
                    <dd><a href="https://2014.igem.org/Team:NJAU_China/Questionnaire Survey">Questionnaire Survey</a></dd>
 +
                    <dd><a href="https://2014.igem.org/Team:NJAU_China/Collaboration">Collaboration</a></dd>
 +
                </dl>
 +
          </li>
 +
          <li><a href="https://2014.igem.org/Team:NJAU_China/Team">Team</a></li>
 +
          <li id="ackonwledgement_li"><a href="#">Acknowledgement</a>
 +
                <dl id="ackonwledgement">
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    <p>As an common pollutant source of industry, low concentration of copper ions can do harm to many creatures. Whereas, <i>Escherichia coli</i> has high tolerance for copper through specific systems. There are <i>cop</i>A promoter,<i>mar</i>O（operator of mar locus） and <i>cue</i>O promoter existing in <i>E.Coli</i>, all of which are directly or indirectly regulated by copper ions. The most sensitive and effective one (<i>cop</i>A)was chosen to trigger the sequential reactions. Glutathione is an intracellular redox-active tripeptide thiol which can effectively detoxify the metal ions by chelation. It can be synthesized by <i>gsh</i>F, an coding sequence derived from <i>Streptococcus thermophilus</i>, which can unify both necessary reactions in one platform.In addition, we also utilize sgRNA-dCas9 system to enhance the efficiency of chelate cupric and cuprous by repressing the expression of <i>cop</i>A. With all these design, an automatically and efficiently copper-preventing guard have been created successfully. </p>
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<p align="left">The primary goal of this project is to aid CO2-capturing efforts by developing a chemically inducible gene expression system for the photosynthesizing cyanobacterium <i>Synechocystis sp. </i> PCC6803, and using it to increase the rate of carbon fixation in the bacterium. </p>
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<p align="left"> During the course of this project, our team will construct a new BioBrick that allows chemically inducible expression of transgenes in <a href="http://www.genome.jp/kegg-bin/show_organism?org=syn"><i>Synechocystis sp.</i> PCC6803.</a></p>
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Latest revision as of 17:03, 19 November 2014

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Abstract

As an common pollutant source of industry, low concentration of copper ions can do harm to many creatures. Whereas, Escherichia coli has high tolerance for copper through specific systems. There are copA promoter,marO（operator of mar locus） and cueO promoter existing in E.Coli, all of which are directly or indirectly regulated by copper ions. The most sensitive and effective one (copA)was chosen to trigger the sequential reactions. Glutathione is an intracellular redox-active tripeptide thiol which can effectively detoxify the metal ions by chelation. It can be synthesized by gshF, an coding sequence derived from Streptococcus thermophilus, which can unify both necessary reactions in one platform.In addition, we also utilize sgRNA-dCas9 system to enhance the efficiency of chelate cupric and cuprous by repressing the expression of copA. With all these design, an automatically and efficiently copper-preventing guard have been created successfully.

njaushengke

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