Team:NJAU China

From 2014.igem.org

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<!DOCTYPE html>
 
<html lang="en">
<html lang="en">
-
  <head>
+
<head>
     <meta charset="utf-8">
     <meta charset="utf-8">
     <meta http-equiv="X-UA-Compatible" content="IE=edge">
     <meta http-equiv="X-UA-Compatible" content="IE=edge">
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     <meta name="author" content="">
     <meta name="author" content="">
     <link rel="icon" href="../../favicon.ico">
     <link rel="icon" href="../../favicon.ico">
 +
    <title>Home</title>
 +
    <script src="https://2014.igem.org/Team:NJAU_China/jquery.min.js?action=raw"></script>
 +
    <script src="https://2014.igem.org/Team:NJAU_China/bootstrap.min.js?action=raw"></script>
-
     <title>Carousel Template for Bootstrap</title>
+
     <link rel="stylesheet" type="text/css"
-
 
+
          href="https://2014.igem.org/Team:NJAU_China/bootstrap.min.css?action=raw&amp;ctype=text/css">
-
 
+
    <link rel="stylesheet" type="text/css"
-
 
+
          href="https://2014.igem.org/Team:NJAU_China/bootstrap-theme.min.css?action=raw&amp;ctype=text/css">
-
    <!-- �� Bootstrap ���� CSS �ļ� -->
+
-
<link rel="stylesheet" href="http://cdn.bootcss.com/bootstrap/3.2.0/css/bootstrap.min.css">
+
-
 
+
-
 
+
-
 
+
-
<!-- jQuery�ļ��������bootstrap.min.js ֮ǰ���� -->
+
-
<script src="http://cdn.bootcss.com/jquery/1.11.1/jquery.min.js"></script>
+
-
 
+
-
<!-- ���µ� Bootstrap ���� JavaScript �ļ� -->
+
-
<script src="http://cdn.bootcss.com/bootstrap/3.2.0/js/bootstrap.min.js"></script>
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-
 
+
     <!-- HTML5 shim and Respond.js IE8 support of HTML5 elements and media queries -->
     <!-- HTML5 shim and Respond.js IE8 support of HTML5 elements and media queries -->
     <!--[if lt IE 9]>
     <!--[if lt IE 9]>
-
      <script src="https://oss.maxcdn.com/html5shiv/3.7.2/html5shiv.min.js"></script>
+
    <script src="https://oss.maxcdn.com/html5shiv/3.7.2/html5shiv.min.js"></script>
-
      <script src="https://oss.maxcdn.com/respond/1.4.2/respond.min.js"></script>
+
    <script src="https://oss.maxcdn.com/respond/1.4.2/respond.min.js"></script>
     <![endif]-->
     <![endif]-->
     <!-- Custom styles for this template -->
     <!-- Custom styles for this template -->
     <link href="carousel.css" rel="stylesheet">
     <link href="carousel.css" rel="stylesheet">
-
  </head>
+
    <style type="text/css">
-
<!-- NAVBAR
+
        body {
-
================================================== -->
+
            padding-bottom: 40px;
-
  <body>
+
            background:#a2dcf3;
-
    <div class="navbar-wrapper">
+
            color: #5a5a5a;
-
      <div class="container">
+
            font-size: 14px;
 +
            font-family:Andalus;
 +
        }
 +
        #myheader{
 +
            width:1366px;
 +
    height:500px;
 +
    margin:0 auto;
 +
    position:relative;
 +
    font-family:Arial, Helvetica, sans-serif;
 +
            background:#a2dcf3 url("https://static.igem.org/mediawiki/2014/3/34/Header_bg.png");
 +
        }
 +
        #contentSub, #footer-box, #catlinks, #search-controls, #p-logo, .printfooter, .visualClear {
 +
            display: none;
 +
        }
-
<div class="navbar navbar-fixed-top navbar-inverse" role="navigation">
+
        /*-- hides default wiki settings --*/
-
      <div class="container">
+
         .firstHeading {
-
         <div class="navbar-header">
+
             width: 975px;
-
          <button type="button" class="navbar-toggle collapsed" data-toggle="collapse" data-target=".navbar-collapse">
+
             margin: 0px auto;
-
             <span class="sr-only">Toggle navigation</span>
+
             padding-top: 100px;
-
             <span class="icon-bar"></span>
+
             margin-bottom: 20px;
-
             <span class="icon-bar"></span>
+
            font-family: Georgia, Times, "Andalus", serif;
-
             <span class="icon-bar"></span>
+
         }
-
          </button>
+
-
          <a class="navbar-brand" href="#">Project name</a>
+
-
         </div>
+
-
        <div class="collapse navbar-collapse">
+
-
          <ul class="nav navbar-nav">
+
-
            <li class="active"><a href="#">Home</a></li>
+
-
            <li><a href="#about">About</a></li>
+
-
            <li><a href="#contact">Contact</a></li>
+
-
          </ul>
+
-
        </div><!-- /.nav-collapse -->
+
-
      </div><!-- /.container -->
+
-
    </div><!-- /.navbar -->
+
-
      </div>
+
        h1, h2, h3, h4, h5 {font-family: Georgia, Times, "Andalus", serif;}
-
    </div>
+
        #top-section {
 +
            /*-- styling for default menu bar (edit, page, history, etc.) --*/ background-color: #383838;border: 0 none;height: 14px;z-index: 100;top: 0;position: fixed; width: 975px;left: 50%;margin-left: -487px;}
 +
#top-section-bar {
 +
            /*-- styling full width bar which hides behind default menu bar (edit, page, history, etc.) --*/
 +
  background-color: #383838;
 +
  height: 14px;
 +
  display: block;
 +
  z-index: 10;position: fixed; width: 100%; top: 0;}
 +
  body { background-color: #fff;
 +
  }
 +
#globalWrapper, #content {
 +
  /*-- changes default wiki settings --*/
 +
  width: 100%;
 +
  height: 100%;
 +
  border: 0px;
 +
  background-color: transparent;
 +
  margin: 0px;
 +
  padding: 0px;
 +
  }
 +
  html, body, .wrapper {
 +
  /*-- changes default wiki settings --*/
 +
  width: 100%;
 +
  height: 100%;
 +
  background-color: transparent;
 +
  } #container {
 +
  /*-- creates container for all content on page --*/
 +
  font-family: Arial, Helvetica, sans-serif;
 +
            font-weight: normal;
 +
            font-size: 14px;
 +
            color: #414141;
 +
            width: 960px;
 +
            margin-left: auto;
 +
            margin-right: auto;
 +
            background-color: #transparent;
 +
            margin-top: 0px;}
 +
.container {
 +
            font-size: 14px;
 +
            width:700px;
 +
            float:right;}
 +
.buttontext { color: #FFF}
 +
#my_h1{position:absolute;top:70px;left:170px;border:none;}
 +
#my_h1 a{display:block;width:190px;height:130px;text-indent:-9999px;}
 +
#my_h2{position:absolute;top:120px;left:370px;border:none;}
 +
#my_h2 a{display:block;width:390px;height:230px;text-indent:-9999px;}
 +
#my_nav{position:absolute;top:402px;left:200px;width:990px;height:50px;}
 +
#my_nav ul{width:100%;margin:0;padding:0;list-style:none;font-size:24px;}
 +
#my_nav ul li{display::inline-block;padding:9px 30px;float:left;list-style:none;position:relative;}
 +
#my_nav ul li:hover{background:#e3f7ff;}#my_nav ul li a{color:#00586b;text-decoration:none;}#project{display:none;position:absolute;z-index:100;top:47px;left:0px;background:#CF6;width:100px;}#project dd{width:135px;font-size:16px;
 +
background:#c9efff;height:40px;margin:0;}#project dd a{width:100%;height:40px;display:block;text-decoration:none;text-align:center;line-height:40px;}#project dd a:hover{background:#a1dbf0;}#achievement{display:none;position:absolute;top:47px;z-index:100;left:0px;background:#CF6;width:100px;}#achievement dd{width:198px;font-size:16px;background:#c9efff;height:40px;margin:0;}#achievement dd a{width:100%;height:40px;display:block;text-decoration:none;text-align:center;line-height:40px;}#achievement dd a:hover{background:#a1dbf0;}#prctice{display:none;position:absolute;top:47px;z-index:100;left:0px;background:#CF6;width:100px;}#prctice dd{width:300px;font-size:16px;background:#c9efff;height:40px;margin:0;}#prctice dd a{width:100%;height:40px;display:block;text-decoration:none;text-align:center;line-height:40px;}#prctice dd a:hover{background:#a1dbf0;}#ackonwledgement{display:none;position:absolute;top:47px;z-index:100;left:0px;background:#CF6;width:100px;}#ackonwledgement dd{width:257px;font-size:16px;background:#c9efff;height:40px;margin:0;}#ackonwledgement dd a{width:100%;height:40px;display:block;text-decoration:none;text-align:center;line-height:40px;}#ackonwledgement dd a:hover{background:#a1dbf0;}
 +
#my_content{
 +
width:1030px;
 +
margin:0px auto;}
 +
#my_abstract{
 +
width:300px;
 +
margin:0 0 0 25px;
 +
        float:left;
 +
}
 +
#my_abstract h2{
 +
height:50px;
 +
line-height:50px;
 +
text-indent:50px;
 +
border:none;
 +
background:#999;
 +
margin:0 0 20px 0;
 +
padding:0;
 +
}
 +
#my_abstract p{
 +
text-indent:30px;
 +
height:240px;
 +
overflow-y:scroll;
 +
}
-
    <!-- Carousel
 
-
    ================================================== -->
 
-
    <div id="myCarousel" class="carousel slide" data-ride="carousel">
 
-
      <!-- Indicators -->
 
-
      <ol class="carousel-indicators">
 
-
        <li data-target="#myCarousel" data-slide-to="0" class="active"></li>
 
-
        <li data-target="#myCarousel" data-slide-to="1"></li>
 
-
        <li data-target="#myCarousel" data-slide-to="2"></li>
 
-
      </ol>
 
-
      <div class="carousel-inner" role="listbox">
 
-
        <div class="item active text-center">
 
-
          <img src="https://static.igem.org/mediawiki/2014/d/d3/Njau_slide1.jpg" alt="First slide" class="text-center">
 
-
          <div class="container">
 
-
            <div class="carousel-caption">
 
-
              <h1>Example headline.</h1>
 
-
              <p>Note: If you're viewing this page via a <code>file://</code> URL, the "next" and "previous" Glyphicon buttons on the left and right might not load/display properly due to web browser security rules.</p>
 
-
              <p><a class="btn btn-lg btn-primary" href="#" role="button">Sign up today</a></p>
 
-
            </div>
 
-
          </div>
 
-
        </div>
 
-
        <div class="item text-center">
 
-
          <img src="https://static.igem.org/mediawiki/2014/2/24/Njau_slide2.jpg" alt="Second slide">
 
-
          <div class="container">
 
-
            <div class="carousel-caption">
 
-
              <h1>Another example headline.</h1>
 
-
              <p>Cras justo odio, dapibus ac facilisis in, egestas eget quam. Donec id elit non mi porta gravida at eget metus. Nullam id dolor id nibh ultricies vehicula ut id elit.</p>
 
-
              <p><a class="btn btn-lg btn-primary" href="#" role="button">Learn more</a></p>
 
-
            </div>
 
-
          </div>
 
-
        </div>
 
-
        <div class="item text-center">
 
-
          <img src="https://static.igem.org/mediawiki/2014/6/62/Njau_slide3.jpg" alt="Third slide">
 
-
          <div class="container">
 
-
            <div class="carousel-caption">
 
-
              <h1>One more for good measure.</h1>
 
-
              <p>Cras justo odio, dapibus ac facilisis in, egestas eget quam. Donec id elit non mi porta gravida at eget metus. Nullam id dolor id nibh ultricies vehicula ut id elit.</p>
 
-
              <p><a class="btn btn-lg btn-primary" href="#" role="button">Browse gallery</a></p>
 
-
            </div>
 
-
          </div>
 
-
        </div>
 
-
      </div>
 
-
      <a class="left carousel-control" href="#myCarousel" role="button" data-slide="prev">
 
-
        <span class="glyphicon glyphicon-chevron-left"></span>
 
-
        <span class="sr-only">Previous</span>
 
-
      </a>
 
-
      <a class="right carousel-control" href="#myCarousel" role="button" data-slide="next">
 
-
        <span class="glyphicon glyphicon-chevron-right"></span>
 
-
        <span class="sr-only">Next</span>
 
-
      </a>
 
-
    </div><!-- /.carousel -->
 
-
    <!-- Marketing messaging and featurettes
+
#my_contents{
-
    ================================================== -->
+
width:1030px;
-
    <!-- Wrap the rest of the page in another container to center all the content. -->
+
margin:30px auto;
 +
height:800px;
 +
}
 +
#my_con{
 +
width:1000px;
 +
height:796px;
 +
float:right;
 +
position:relative;
 +
border:2px solid #999;
 +
        margin:20px 10px 0 0;
 +
}
 +
#stage_1{
 +
width:20px;
 +
height:20px;
 +
position:absolute;
 +
top:40px;
 +
left:40px;
 +
border-left:2px solid #333;
 +
border-top:2px solid #333;
 +
}
 +
#stage_2{
 +
width:20px;
 +
height:20px;
 +
position:absolute;
 +
top:40px;
 +
left:940px;
 +
border-right:2px solid #333;
 +
border-top:2px solid #333;
 +
}
 +
#stage_3{
 +
width:20px;
 +
height:20px;
 +
position:absolute;
 +
top:740px;
 +
left:40px;
 +
border-bottom:2px solid #333;
 +
border-left:2px solid #333;
 +
}
 +
#stage_4{
 +
width:20px;
 +
height:20px;
 +
position:absolute;
 +
top:740px;
 +
left:940px;
 +
border-right:2px solid #333;
 +
border-bottom:2px solid #333;
 +
}
 +
#real_con{
 +
position:absolute;
 +
top:60px;
 +
left:60px;
 +
width:880px;
 +
height:680px;
 +
}
 +
#real_con ul{
 +
list-style:none;
 +
width:100%;
 +
height:100%;
 +
margin:0;
 +
padding:0;
 +
}
 +
#real_con ul li{
 +
width:400px;
 +
height:300px;
 +
float:left;
 +
background:#0F0;
 +
margin:26px 0 0 26px;}
 +
#real_con ul li#one_1{
 +
background:url("https://static.igem.org/mediawiki/2014/b/b4/Project_li_bg.png") no-repeat;}
 +
#real_con ul li#two_2{
 +
background:url("https://static.igem.org/mediawiki/2014/1/17/Model_li_bg.png") no-repeat;}
 +
#real_con ul li#three_3{
 +
background:url("https://static.igem.org/mediawiki/2014/6/6e/Weblab_li_bg.png") no-repeat;}
 +
#real_con ul li#four_4{
 +
background:url("https://static.igem.org/mediawiki/2014/f/f0/Practice_li_bg.png") no-repeat;}
 +
#real_con ul li a{
 +
text-decoration:none;
 +
display:block;
 +
width:100%;
 +
height:100%;
 +
}
 +
#real_con ul li a#one:hover{
 +
background:url("https://static.igem.org/mediawiki/2014/6/68/Project_a_bg.png") no-repeat;}
-
    <div class="container marketing">
+
#real_con ul li a#two:hover{
 +
background:url("https://static.igem.org/mediawiki/2014/d/dc/Model_a_bg.png") no-repeat;}
 +
#real_con ul li a#three:hover{
 +
background:url("https://static.igem.org/mediawiki/2014/6/60/Weblab_a_bg.png") no-repeat;}
 +
#real_con ul li a#four:hover{
 +
background:url("https://static.igem.org/mediawiki/2014/7/7f/Practice_a_bg.png") no-repeat;}
-
      <!-- Three columns of text below the carousel -->
 
-
      <div class="row">
 
-
        <div class="col-lg-4">
 
-
          <img class="img-circle" src="https://static.igem.org/mediawiki/2014/c/c3/Avatar1.jpg" alt="Generic placeholder image" style="width: 140px; height: 140px;">
 
-
          <h2>Heading</h2>
 
-
          <p>Donec sed odio dui. Etiam porta sem malesuada magna mollis euismod. Nullam id dolor id nibh ultricies vehicula ut id elit. Morbi leo risus, porta ac consectetur ac, vestibulum at eros. Praesent commodo cursus magna.</p>
 
-
          <p><a class="btn btn-default" href="#" role="button">View details &raquo;</a></p>
 
-
        </div><!-- /.col-lg-4 -->
 
-
        <div class="col-lg-4">
 
-
          <img class="img-circle" src="https://static.igem.org/mediawiki/2014/e/e8/Avatar2.jpg" alt="Generic placeholder image" style="width: 140px; height: 140px;">
 
-
          <h2>Heading</h2>
 
-
          <p>Duis mollis, est non commodo luctus, nisi erat porttitor ligula, eget lacinia odio sem nec elit. Cras mattis consectetur purus sit amet fermentum. Fusce dapibus, tellus ac cursus commodo, tortor mauris condimentum nibh.</p>
 
-
          <p><a class="btn btn-default" href="#" role="button">View details &raquo;</a></p>
 
-
        </div><!-- /.col-lg-4 -->
 
-
        <div class="col-lg-4">
 
-
          <img class="img-circle" src="https://static.igem.org/mediawiki/2014/4/46/Avatar3.jpg" alt="Generic placeholder image" style="width: 140px; height: 140px;">
 
-
          <h2>Heading</h2>
 
-
          <p>Donec sed odio dui. Cras justo odio, dapibus ac facilisis in, egestas eget quam. Vestibulum id ligula porta felis euismod semper. Fusce dapibus, tellus ac cursus commodo, tortor mauris condimentum nibh, ut fermentum massa justo sit amet risus.</p>
 
-
          <p><a class="btn btn-default" href="#" role="button">View details &raquo;</a></p>
 
-
        </div><!-- /.col-lg-4 -->
 
-
      </div><!-- /.row -->
 
 +
#thanks{
 +
height:177px;
 +
width:1030px;
 +
padding:30px 0 0 0;
 +
        clear:both;
 +
margin:30px auto 0;}
-
      <!-- START THE FEATURETTES -->
 
-
      <hr class="featurette-divider">
+
    </style>
 +
    <style>
 +
        #contentSub, #footer-box, #catlinks, #search-controls, #p-logo, .printfooter, .firstHeading, .visualClear {
 +
            display: none;
 +
        }
-
      <div class="row featurette">
+
         /*-- hides default wiki settings --*/
-
         <div class="col-md-7">
+
    </style>
-
          <h2 class="featurette-heading">First featurette heading. <span class="text-muted">It'll blow your mind.</span></h2>
+
<script type="text/javascript">
-
          <p class="lead">Donec ullamcorper nulla non metus auctor fringilla. Vestibulum id ligula porta felis euismod semper. Praesent commodo cursus magna, vel scelerisque nisl consectetur. Fusce dapibus, tellus ac cursus commodo.</p>
+
window.onload = function(){
-
        </div>
+
xialashow('project_li','project');
-
        <div class="col-md-5">
+
xialahidden('project_li','project');
-
          <img class="featurette-image img-responsive" data-src="holder.js/500x500/auto" alt="Generic placeholder image">
+
xialashow('achievement_li','achievement');
-
        </div>
+
xialahidden('achievement_li','achievement');
-
      </div>
+
xialashow('prctice_li','prctice');
 +
xialahidden('prctice_li','prctice');
 +
xialashow('ackonwledgement_li','ackonwledgement');
 +
xialahidden('ackonwledgement_li','ackonwledgement');
 +
}
 +
function xialashow(li_id,dl_id){
 +
var li_id = document.getElementById(li_id);
 +
var dl_id = document.getElementById(dl_id);
 +
li_id.onmouseover = function(){
 +
dl_id.style.display = 'block';
 +
}
 +
}
 +
function xialahidden(li_id,dl_id){
 +
var li_id = document.getElementById(li_id);
 +
var dl_id = document.getElementById(dl_id);
 +
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    <p>As an common pollutant source of industry, low concentration of copper ions can do harm to many creatures. Whereas, <i>Escherichia coli</i> has high tolerance for copper through specific systems. There are <i>cop</i>A promoter,<i>mar</i>O（operator of mar locus） and <i>cue</i>O promoter existing in <i>E.Coli</i>, all of which are directly or indirectly regulated by copper ions. The most sensitive and effective one (<i>cop</i>A)was chosen to trigger the sequential reactions. Glutathione is an intracellular redox-active tripeptide thiol which can effectively detoxify the metal ions by chelation. It can be synthesized by <i>gsh</i>F, an coding sequence derived from <i>Streptococcus thermophilus</i>, which can unify both necessary reactions in one platform.In addition, we also utilize sgRNA-dCas9 system to enhance the efficiency of chelate cupric and cuprous by repressing the expression of <i>cop</i>A. With all these design, an automatically and efficiently copper-preventing guard have been created successfully. </p>
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Latest revision as of 17:03, 19 November 2014

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Abstract

As an common pollutant source of industry, low concentration of copper ions can do harm to many creatures. Whereas, Escherichia coli has high tolerance for copper through specific systems. There are copA promoter,marO（operator of mar locus） and cueO promoter existing in E.Coli, all of which are directly or indirectly regulated by copper ions. The most sensitive and effective one (copA)was chosen to trigger the sequential reactions. Glutathione is an intracellular redox-active tripeptide thiol which can effectively detoxify the metal ions by chelation. It can be synthesized by gshF, an coding sequence derived from Streptococcus thermophilus, which can unify both necessary reactions in one platform.In addition, we also utilize sgRNA-dCas9 system to enhance the efficiency of chelate cupric and cuprous by repressing the expression of copA. With all these design, an automatically and efficiently copper-preventing guard have been created successfully.

njaushengke

Retrieved from "http://2014.igem.org/Team:NJAU_China"