Latest revision as of 14:44, 28 November 2014

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Welcome to our project

Results of module 1 - rMFC

Results of module 2 - CO₂ fixation

Results of module 3 - Isobutanol production

Results of our biosafety

SYNENERGENE/ Applications

Our project

Ecological power management is currently facing three challenges: the storage of electrical power, the development of renewable energy resources, which are not timely and locally synced with demand, and the increase of atmospheric carbon dioxide due to the use of fossil fuels. We address these challenges by implementing a proof of concept production of the biofuel isobutanol from carbon dioxide. In our project, a bacterial microcompartment from cyanobacteria, the carboxysome, was deployed in Escherichia. coli for carbon dioxide fixation under aerobic conditions. We further engineered E. coli to derive the energy for this process from electricity by implementing electron-mediator uptake and recycling. This ‘electricity’ pathway comprised proteins such as the furmarate reductase. Feasibility was analyzed in a self-constructed bioreactor. Ultimately, we established an isobutanol production pathway by heterologous expression of five genes from E. coli, Bacillus subtilis and Lactococcus lactis.

Achievements

	Design and construction of a rMFC
	Design and construction of a flow cell reactor
	Construction of an electrophilic E.coli strain
	Successful isobutanol production
	Construction of a functional microcompartment, the carboxysome
	Genome integration of OprF
	Functional in-vitro assay of RuBisCo
	Development of an antibiotic-free selection system
	Several human practice projects
	SYNENERGENE cooperation
	Help another team

@@ Line 124: / Line 124: @@
 <h6>Our project</h6>
 <p>
-The supply with electricity is facing three major problems: the storage of electricity, the increasing amount of atmospheric carbon dioxide and the development of alternative energy sources. Therefore we want to implement a proof of concept for the production of the biofuel isobutanol from carbon dioxide. A bacterial microcompartment originated from cyanobacteria, called carboxysome, is used to realize the fixation of carbon dioxide in <i>Escherichia coli</i>. Within this microcompartment the carbon dioxide fixation is enabled under aerobic conditions. The needed energy for this process is provided by electricity. The electrons are transferred with a mediator into the cells and its uptake is empowered by different modifications of <i>E. coli</i>, like over-expression of the fumarate reductase. This concept is realized in a self-constructed bioreactor. Finally the production of isobutanol is established by hetereologous expression of various enzymes from different species.</p><br>
+Ecological power management is currently facing three challenges: the storage of electrical power, the development of renewable energy resources, which are not timely and locally synced with demand, and the increase of atmospheric carbon dioxide due to the use of fossil fuels. We address these challenges by implementing a proof of concept production of the biofuel isobutanol from carbon dioxide. In our project, a bacterial microcompartment from cyanobacteria, the carboxysome, was deployed in <i>Escherichia.&nbsp;coli</i> for carbon dioxide fixation under aerobic conditions. We further engineered <i>E.&nbsp;coli</i> to derive the energy for this process from electricity by implementing electron-mediator uptake and recycling. This ‘electricity’ pathway comprised proteins such as the furmarate reductase. Feasibility was analyzed in a self-constructed bioreactor. Ultimately, we established an isobutanol production pathway by heterologous expression of five genes from <i>E.&nbsp;coli</i>, <i>Bacillus&nbsp;subtilis</i> and <i>Lactococcus&nbsp;lactis</i>.
+</p><br>
 </div>
 </div>
@@ Line 163: / Line 164: @@
 <tr><td><img src="https://static.igem.org/mediawiki/2014/2/2a/Bielefeld_CeBiTec_2014-10-17_Haken.png" width="50px"></td><td>Construction of an <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Project/rMFC/Mediators" target="_blank">electrophilic <i>E.coli</i> strain</a></td></tr>
 <tr><td><img src="https://static.igem.org/mediawiki/2014/2/2a/Bielefeld_CeBiTec_2014-10-17_Haken.png" width="50px"></td><td>Successful <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Results/Pathway" target="_blank">isobutanol production</a></td></tr>
-<tr><td><img src="https://static.igem.org/mediawiki/2014/2/2a/Bielefeld_CeBiTec_2014-10-17_Haken.png" width="50px"></td><td>Construction of a functional microcompartment, the carboxysome</td></tr>
+<tr><td><img src="https://static.igem.org/mediawiki/2014/2/2a/Bielefeld_CeBiTec_2014-10-17_Haken.png" width="50px"></td><td>Construction of a functional microcompartment, the <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Results/CO2-fixation/Carboxysome" target="_blank">carboxysome</a></td></tr>
-<tr><td><img src="https://static.igem.org/mediawiki/2014/2/2a/Bielefeld_CeBiTec_2014-10-17_Haken.png" width="50px"></td><td>Genome integration of OprF</td></tr>
+<tr><td><img src="https://static.igem.org/mediawiki/2014/2/2a/Bielefeld_CeBiTec_2014-10-17_Haken.png" width="50px"></td><td>Genome integration of <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Results/rMFC" target="_blank">OprF</a></td></tr>
-<tr><td><img src="https://static.igem.org/mediawiki/2014/2/2a/Bielefeld_CeBiTec_2014-10-17_Haken.png" width="50px"></td><td>Functional <i>in-vitro</i> assay of RuBisCo</td></tr>
+<tr><td><img src="https://static.igem.org/mediawiki/2014/2/2a/Bielefeld_CeBiTec_2014-10-17_Haken.png" width="50px"></td><td>Functional <i>in-vitro</i> assay of <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Results/CO2-fixation/RuBisCO" target"=_blank">RuBisCo</a></td></tr>
-<tr><td><img src="https://static.igem.org/mediawiki/2014/2/2a/Bielefeld_CeBiTec_2014-10-17_Haken.png" width="50px"></td><td>Development of an antibiotic-free selection system</td></tr>
+<tr><td><img src="https://static.igem.org/mediawiki/2014/2/2a/Bielefeld_CeBiTec_2014-10-17_Haken.png" width="50px"></td><td>Development of an <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Results/Biosafety" target="_blank">antibiotic-free selection system</a></td></tr>
 <tr><td><img src="https://static.igem.org/mediawiki/2014/2/2a/Bielefeld_CeBiTec_2014-10-17_Haken.png" width="50px"></td><td>
-Several human practice projects</td></tr>
+Several <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/HumanPractice" target="_blank">human practice projects</a></td></tr>
 <tr><td><img src="https://static.igem.org/mediawiki/2014/2/2a/Bielefeld_CeBiTec_2014-10-17_Haken.png" width="50px"></td><td>
-SYNENERGENE cooperation</td></tr>
+<a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/HumanPractice/Synenergene" target="_blank">SYNENERGENE cooperation</a></td></tr>
 <tr><td><img src="https://static.igem.org/mediawiki/2014/2/2a/Bielefeld_CeBiTec_2014-10-17_Haken.png" width="50px"></td><td>
-Help another team</td></tr>
+<a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/HumanPractice/Wikisession" target="_blank">Help another team</a></td></tr>
 </table>
 </p>