Team:UT-Dallas/temp

From 2014.igem.org

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<section id="titlechart"></html>{{Header_menu}}<html><div class="page_content"><br><h2>Safety</H2><p style="display:block">
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<section id="titlechart"></html>{{Header_menu}}<html><div class="page_content"><br><h2>Parts</H2><p style="display:block">
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<br> Before beginning work on the project, every lab member underwent training with the Advisor on various safety protocols and proper techniques to be followed in lab, including how to properly handle biological organisms and parts, how to use equipment safely, measures to keep the lab area clean and precautions when using carcinogenic materials such as ethidium bromide. Students were advised to wear long pants, close-toes shoes, and appropriate personal protective equipment. This training followed the requirements outlined in the laboratory safety training requirement of UT Dallas, which can be found at http://www.utdallas.edu/research/orc/lab_safety_training.
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<br><img src="https://static.igem.org/mediawiki/2014/4/48/GRNA_Library.png" width="1024px">
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<br><br>
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E. coli strain DH5a was acquired from NEB and used as a chassis throughout the project. The species falls under Risk Group 1 as classified by the American Biological Safety Association (ABSA) and can potentially cause skin and eye irritation in humans. Had our project progressed to further stages, the use of Vibrio cholerae would have been required, which is in Risk Group 2 and poses greater risk to humans, requiring more precaution in handling it.
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<br><h2>Local Rules and Regulations </H2><br><p style="display:block">
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<br><h2>Parts List</H2><br><p style="display:block">
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<font color="black" style="font-weight:bold;font-size:20px">Who is responsible for biological safety at your institution?</font>
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<table style="border:1px solid black;" width="1024px" align="center">
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<br> Sanaz Okhovat and Shane Solis at the Office of Research Compliance are in charge of biology safety at UTD. <br><br>
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<tbody><tr style="border:1px solid black;">
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<font color="black" style="font-weight:bold;font-size:20px"> What are the biosafety guidelines of your institution? Please give a link to these guidelines. </font>
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<td style="border:1px solid black;">Name</td>
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<br> <a href="http://www.utdallas.edu/ehs/manuals/docs/BioSafety%20Manual.pdf" style="font-size:20px">http://www.utdallas.edu/ehs/manuals/docs/BioSafety%20Manual.pdf</a><br><br>
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<td style="border:1px solid black;">Type</td>
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<font color="black" style="font-weight:bold;font-size:20px"> In your country, what are the regulations that govern biosafety in research laboratories? Please give a link to these regulations.</font>
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<td style="border:1px solid black;">Description</td>
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<br><a href="http://www.cdc.gov/biosafety/publications/bmbl5/bmbl.pdf " style="font-size:20px">http://www.cdc.gov/biosafety/publications/bmbl5/bmbl.pdf </a>
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<td style="border:1px solid black;">Length</td>
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<br><br>text2
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</tr>
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<tr style="border:1px solid black;">
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<td style="border:1px solid black;"><a href="http://parts.igem.org/Part:BBa_K1487021"> BBa_K1487021</a> </td>
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<td style="border:1px solid black;">Composite</td><td style="border:1px solid black;">P-acfA gRNA</td><td style="border:1px solid black;">145</td>
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</tr>
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<tr>
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<td style="border:1px solid black;"><a href="http://parts.igem.org/Part:BBa_K1487022"> BBa_K1487022</a> </td>
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<td style="border:1px solid black;">Composite</td><td style="border:1px solid black;">P-acfB gRNA</td><td style="border:1px solid black;">145</td>
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</tr>
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<tr>
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<td style="border:1px solid black;"><a href="http://parts.igem.org/Part:BBa_K1487023"> BBa_K1487023</a></td>
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<td style="border:1px solid black;">Composite</td><td style="border:1px solid black;">P-acfC gRNA</td><td style="border:1px solid black;">145</td>
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</tr>
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<tr>
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<td style="border:1px solid black;"><a href="http://parts.igem.org/Part:BBa_K1487024"> BBa_K1487024</a></td>
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<td style="border:1px solid black;">Composite</td><td style="border:1px solid black;">P-acfD gRNA</td><td style="border:1px solid black;">145</td>
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</tr>
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<tr>
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<td style="border:1px solid black;"><a href="http://parts.igem.org/Part:BBa_K1487025"> BBa_K1487025</a></td>
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<td style="border:1px solid black;">Composite</td><td style="border:1px solid black;">P-ctxA gRNA</td><td style="border:1px solid black;">145</td>
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</tr>
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<tr>
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<td style="border:1px solid black;"><a href="http://parts.igem.org/Part:BBa_K1487026"> BBa_K1487026</a></td>
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<td style="border:1px solid black;">Composite</td><td style="border:1px solid black;">P-ctxB gRNA</td><td style="border:1px solid black;">145</td>
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</tr>
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<tr>
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<td style="border:1px solid black;"><a href="http://parts.igem.org/Part:BBa_K1487027"> BBa_K1487027</a></td>
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<td style="border:1px solid black;">Composite</td><td style="border:1px solid black;">P-tcpA gRNA</td><td style="border:1px solid black;">145</td>
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</tr>
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<tr>
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<td style="border:1px solid black;"><a href="http://parts.igem.org/Part:BBa_K1487028"> BBa_K1487028</a></td>
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<td style="border:1px solid black;">Composite</td><td style="border:1px solid black;">P-tcpB gRNA</td><td style="border:1px solid black;">145</td>
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</tr>
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<tr>
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<td style="border:1px solid black;"><a href="http://parts.igem.org/Part:BBa_K1487029"> BBa_K1487029</a></td>
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<td style="border:1px solid black;">Composite</td><td style="border:1px solid black;">P-tcpC gRNA</td><td style="border:1px solid black;">145</td>
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</tr>
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<tr>
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<td style="border:1px solid black;"><a href="http://parts.igem.org/Part:BBa_K1487030"> BBa_K1487030</a></td>
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<td style="border:1px solid black;">Composite</td><td style="border:1px solid black;">P-tcpD gRNA</td><td style="border:1px solid black;">145</td>
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</tr>
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<tr>
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<td style="border:1px solid black;"><a href="http://parts.igem.org/Part:BBa_K1487031"> BBa_K1487031</a></td>
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<td style="border:1px solid black;">Composite</td><td style="border:1px solid black;">P-tcpE gRNA</td><td style="border:1px solid black;">145</td>
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</tr>
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<tr>
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<td style="border:1px solid black;"><a href="http://parts.igem.org/Part:BBa_K1487032"> BBa_K1487032</a></td>
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<td style="border:1px solid black;">Composite</td><td style="border:1px solid black;">P-tcpF gRNA</td><td style="border:1px solid black;">145</td>
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</tr>
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<tr>
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<td style="border:1px solid black;"><a href="http://parts.igem.org/Part:BBa_K1487033"> BBa_K1487033</a></td>
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<td style="border:1px solid black;">Composite</td><td style="border:1px solid black;">P-tcpH gRNA</td><td style="border:1px solid black;">145</td>
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</tr>
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<tr>
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<td style="border:1px solid black;"><a href="http://parts.igem.org/Part:BBa_K1487034"> BBa_K1487034</a></td>
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<td style="border:1px solid black;">Composite</td><td style="border:1px solid black;">P-tcpI gRNA</td><td style="border:1px solid black;">145</td>
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</tr>
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<tr>
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<td style="border:1px solid black;"><a href="http://parts.igem.org/Part:BBa_K1487035"> BBa_K1487035</a></td>
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<td style="border:1px solid black;">Composite</td><td style="border:1px solid black;">P-tcpJ gRNA</td><td style="border:1px solid black;">145</td>
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</tr>
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<tr>
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<td style="border:1px solid black;"><a href="http://parts.igem.org/Part:BBa_K1487036"> BBa_K1487036</a></td>
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<td style="border:1px solid black;">Composite</td><td style="border:1px solid black;">P-tcpP gRNA</td><td style="border:1px solid black;">145</td>
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</tr>
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<tr>
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<td style="border:1px solid black;"><a href="http://parts.igem.org/Part:BBa_K1487037"> BBa_K1487037</a></td>
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<td style="border:1px solid black;">Composite</td><td style="border:1px solid black;">P-tcpQ gRNA</td><td style="border:1px solid black;">143</td>
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</tr>
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<tr>
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<td style="border:1px solid black;"><a href="http://parts.igem.org/Part:BBa_K1487038"> BBa_K1487038</a></td>
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<td style="border:1px solid black;">Composite</td><td style="border:1px solid black;">P-tcpR gRNA</td><td style="border:1px solid black;">145</td>
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</tr>
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<tr>
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<td style="border:1px solid black;"><a href="http://parts.igem.org/Part:BBa_K1487039"> BBa_K1487039</a></td>
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<td style="border:1px solid black;">Composite</td><td style="border:1px solid black;">P-tcpS gRNA</td><td style="border:1px solid black;">145</td>
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</tr>
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<tr>
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<td style="border:1px solid black;"><a href="http://parts.igem.org/Part:BBa_K1487040"> BBa_K1487040</a></td>
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<td style="border:1px solid black;">Composite</td><td style="border:1px solid black;">P-tcpT gRNA</td><td style="border:1px solid black;">145</td>
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</tr>
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<tr>
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<td style="border:1px solid black;"><a href="http://parts.igem.org/Part:BBa_K1487041"> BBa_K1487041</a></td>
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<td style="border:1px solid black;">Composite</td><td style="border:1px solid black;">P-toxT gRNA</td><td style="border:1px solid black;">145</td>
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</tr>
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<tr>
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<td style="border:1px solid black;"><a href="http://parts.igem.org/Part:BBa_K1487063"> BBa_K1487063</a></td>
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<td style="border:1px solid black;">Composite</td><td style="border:1px solid black;">P + t.seq(acfA) - YFP</td><td style="border:1px solid black;">807</td>
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</tr>
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<tr>
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<td style="border:1px solid black;"><a href="http://parts.igem.org/Part:BBa_K1487064"> BBa_K1487064</a></td>
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<td style="border:1px solid black;">Composite</td><td style="border:1px solid black;">P + t.seq(acfB) - YFP</td><td style="border:1px solid black;">807</td>
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</tr>
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<tr>
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<td style="border:1px solid black;"><a href="http://parts.igem.org/Part:BBa_K1487065"> BBa_K1487065</a></td>
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<td style="border:1px solid black;">Composite</td><td style="border:1px solid black;">P + t.seq(acfC) - YFP</td><td style="border:1px solid black;">807</td>
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</tr>
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<tr>
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<td style="border:1px solid black;"><a href="http://parts.igem.org/Part:BBa_K1487066"> BBa_K1487066</a></td>
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<td style="border:1px solid black;">Composite</td><td style="border:1px solid black;">P + t.seq(acfD) - YFP</td><td style="border:1px solid black;">807</td>
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</tr>
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<tr>
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<td style="border:1px solid black;"><a href="http://parts.igem.org/Part:BBa_K1487067"> BBa_K1487067</a></td>
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<td style="border:1px solid black;">Composite</td><td style="border:1px solid black;">P + t.seq(ctxA) - YFP</td><td style="border:1px solid black;">807</td>
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</tr>
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<tr>
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<td style="border:1px solid black;"><a href="http://parts.igem.org/Part:BBa_K1487068"> BBa_K1487068</a></td>
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<td style="border:1px solid black;">Composite</td><td style="border:1px solid black;">P + t.seq(ctxB) - YFP</td><td style="border:1px solid black;">807</td>
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</tr>
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<tr>
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<td style="border:1px solid black;"><a href="http://parts.igem.org/Part:BBa_K1487069"> BBa_K1487069</a></td>
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<td style="border:1px solid black;">Composite</td><td style="border:1px solid black;">P + t.seq(tcpA) - YFP</td><td style="border:1px solid black;">807</td>
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</tr>
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<tr>
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<td style="border:1px solid black;"><a href="http://parts.igem.org/Part:BBa_K1487070"> BBa_K1487070</a></td>
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<td style="border:1px solid black;">Composite</td><td style="border:1px solid black;">P + t.seq(tcpB) - YFP</td><td style="border:1px solid black;">807</td>
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</tr>
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<tr>
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<td style="border:1px solid black;"><a href="http://parts.igem.org/Part:BBa_K1487071"> BBa_K1487071</a></td>
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<td style="border:1px solid black;">Composite</td><td style="border:1px solid black;">P + t.seq(tcpC) - YFP</td><td style="border:1px solid black;">807</td>
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</tr>
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<tr>
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<td style="border:1px solid black;"><a href="http://parts.igem.org/Part:BBa_K1487072"> BBa_K1487072</a></td>
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<td style="border:1px solid black;">Composite</td><td style="border:1px solid black;">P + t.seq(tcpD) - YFP</td><td style="border:1px solid black;">807</td>
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</tr>
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<tr>
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<td style="border:1px solid black;"><a href="http://parts.igem.org/Part:BBa_K1487073"> BBa_K1487073</a></td>
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<td style="border:1px solid black;">Composite</td><td style="border:1px solid black;">P + t.seq(tcpE) - YFP</td><td style="border:1px solid black;">807</td>
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</tr>
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<tr>
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<td style="border:1px solid black;"><a href="http://parts.igem.org/Part:BBa_K1487074"> BBa_K1487074</a></td>
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<td style="border:1px solid black;">Composite</td><td style="border:1px solid black;">P + t.seq(tcpF) - YFP</td><td style="border:1px solid black;">807</td>
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</tr>
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<tr>
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<td style="border:1px solid black;"><a href="http://parts.igem.org/Part:BBa_K1487075"> BBa_K1487075</a></td>
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<td style="border:1px solid black;">Composite</td><td style="border:1px solid black;">P + t.seq(tcpH) - YFP</td><td style="border:1px solid black;">807</td>
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</tr>
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<tr>
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<td style="border:1px solid black;"><a href="http://parts.igem.org/Part:BBa_K1487076"> BBa_K1487076</a></td>
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<td style="border:1px solid black;">Composite</td><td style="border:1px solid black;">P + t.seq(tcpI) - YFP</td><td style="border:1px solid black;">807</td>
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</tr>
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<tr>
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<td style="border:1px solid black;"><a href="http://parts.igem.org/Part:BBa_K1487077"> BBa_K1487077</a></td>
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<td style="border:1px solid black;">Composite</td><td style="border:1px solid black;">P + t.seq(tcpJ) - YFP</td><td style="border:1px solid black;">807</td>
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</tr>
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<tr>
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<td style="border:1px solid black;"><a href="http://parts.igem.org/Part:BBa_K1487078"> BBa_K1487078</a></td>
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<td style="border:1px solid black;">Composite</td><td style="border:1px solid black;">P + t.seq(tcpP) - YFP</td><td style="border:1px solid black;">807</td>
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</tr>
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<tr>
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<td style="border:1px solid black;"><a href="http://parts.igem.org/Part:BBa_K1487079"> BBa_K1487079</a></td>
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<td style="border:1px solid black;">Composite</td><td style="border:1px solid black;">P + t.seq(tcpQ) - YFP</td><td style="border:1px solid black;">805</td>
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</tr>
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<tr>
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<td style="border:1px solid black;"><a href="http://parts.igem.org/Part:BBa_K1487080"> BBa_K1487080</a></td>
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<td style="border:1px solid black;">Composite</td><td style="border:1px solid black;">P + t.seq(tcpR) - YFP</td><td style="border:1px solid black;">807</td>
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</tr>
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<tr>
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<td style="border:1px solid black;"><a href="http://parts.igem.org/Part:BBa_K1487081"> BBa_K1487081</a></td>
 +
<td style="border:1px solid black;">Composite</td><td style="border:1px solid black;">P + t.seq(tcpS) - YFP</td><td style="border:1px solid black;">807</td>
 +
</tr>
 +
<tr>
 +
<td style="border:1px solid black;"><a href="http://parts.igem.org/Part:BBa_K1487082"> BBa_K1487082</a></td>
 +
<td style="border:1px solid black;">Composite</td><td style="border:1px solid black;">P + t.seq(tcpT) - YFP</td><td style="border:1px solid black;">807</td>
 +
</tr>
 +
<tr>
 +
<td style="border:1px solid black;"><a href="http://parts.igem.org/Part:BBa_K1487083"> BBa_K1487083</a></td>
 +
<td style="border:1px solid black;">Composite</td><td style="border:1px solid black;">P + t.seq(toxT) - YFP</td><td style="border:1px solid black;">807</td>
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</tr></tbody></table>
</p><br><br>
</p><br><br>
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<br><h2>TITLE 3</H2><br><p style="display:block">
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<img width="1024" src="https://static.igem.org/mediawiki/2014/5/54/CtxB_gRNA_Cutting_Quantitative_data.png">
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Latest revision as of 03:48, 18 October 2014


Parts





Parts List


Name Type Description Length
BBa_K1487021 CompositeP-acfA gRNA145
BBa_K1487022 CompositeP-acfB gRNA145
BBa_K1487023 CompositeP-acfC gRNA145
BBa_K1487024 CompositeP-acfD gRNA145
BBa_K1487025 CompositeP-ctxA gRNA145
BBa_K1487026 CompositeP-ctxB gRNA145
BBa_K1487027 CompositeP-tcpA gRNA145
BBa_K1487028 CompositeP-tcpB gRNA145
BBa_K1487029 CompositeP-tcpC gRNA145
BBa_K1487030 CompositeP-tcpD gRNA145
BBa_K1487031 CompositeP-tcpE gRNA145
BBa_K1487032 CompositeP-tcpF gRNA145
BBa_K1487033 CompositeP-tcpH gRNA145
BBa_K1487034 CompositeP-tcpI gRNA145
BBa_K1487035 CompositeP-tcpJ gRNA145
BBa_K1487036 CompositeP-tcpP gRNA145
BBa_K1487037 CompositeP-tcpQ gRNA143
BBa_K1487038 CompositeP-tcpR gRNA145
BBa_K1487039 CompositeP-tcpS gRNA145
BBa_K1487040 CompositeP-tcpT gRNA145
BBa_K1487041 CompositeP-toxT gRNA145
BBa_K1487063 CompositeP + t.seq(acfA) - YFP807
BBa_K1487064 CompositeP + t.seq(acfB) - YFP807
BBa_K1487065 CompositeP + t.seq(acfC) - YFP807
BBa_K1487066 CompositeP + t.seq(acfD) - YFP807
BBa_K1487067 CompositeP + t.seq(ctxA) - YFP807
BBa_K1487068 CompositeP + t.seq(ctxB) - YFP807
BBa_K1487069 CompositeP + t.seq(tcpA) - YFP807
BBa_K1487070 CompositeP + t.seq(tcpB) - YFP807
BBa_K1487071 CompositeP + t.seq(tcpC) - YFP807
BBa_K1487072 CompositeP + t.seq(tcpD) - YFP807
BBa_K1487073 CompositeP + t.seq(tcpE) - YFP807
BBa_K1487074 CompositeP + t.seq(tcpF) - YFP807
BBa_K1487075 CompositeP + t.seq(tcpH) - YFP807
BBa_K1487076 CompositeP + t.seq(tcpI) - YFP807
BBa_K1487077 CompositeP + t.seq(tcpJ) - YFP807
BBa_K1487078 CompositeP + t.seq(tcpP) - YFP807
BBa_K1487079 CompositeP + t.seq(tcpQ) - YFP805
BBa_K1487080 CompositeP + t.seq(tcpR) - YFP807
BBa_K1487081 CompositeP + t.seq(tcpS) - YFP807
BBa_K1487082 CompositeP + t.seq(tcpT) - YFP807
BBa_K1487083 CompositeP + t.seq(toxT) - YFP807



Retrieved from "http://2014.igem.org/Team:UT-Dallas/temp"