Team:Austin Texas/interlab study
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[[File:interlabliquidcultures.jpg|240px|right|thumb| '''Figure 1.''' Triplicate liquid cultures of each construct.]] | [[File:interlabliquidcultures.jpg|240px|right|thumb| '''Figure 1.''' Triplicate liquid cultures of each construct.]] | ||
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'''1.''' The first device is [http://parts.igem.org/Part:BBa_I20260 BBa_I20260], which is a composite BioBrick part that contains: | '''1.''' The first device is [http://parts.igem.org/Part:BBa_I20260 BBa_I20260], which is a composite BioBrick part that contains: | ||
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*The downstream gene is also a composite of a number of BioBrick parts including an RBS - [http://parts.igem.org/Part:BBa_B0032 B0032], a GFP coding sequence - [http://parts.igem.org/Part:BBa_E0040 E0040], and a transcriptional terminator - [http://parts.igem.org/Part:BBa_B0015 B0015]. | *The downstream gene is also a composite of a number of BioBrick parts including an RBS - [http://parts.igem.org/Part:BBa_B0032 B0032], a GFP coding sequence - [http://parts.igem.org/Part:BBa_E0040 E0040], and a transcriptional terminator - [http://parts.igem.org/Part:BBa_B0015 B0015]. | ||
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'''2.''' The second device was cloned in lab, as described in the interlab study procedure. | '''2.''' The second device was cloned in lab, as described in the interlab study procedure. | ||
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*However, the backbone of this device is different, [http://parts.igem.org/Part:pSB3K3 pSB3K3]. | *However, the backbone of this device is different, [http://parts.igem.org/Part:pSB3K3 pSB3K3]. | ||
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'''3.''' The third device was also cloned in lab, as described in the interlab study procedure. | '''3.''' The third device was also cloned in lab, as described in the interlab study procedure. | ||
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*The 96-well plate was inserted into an Infinite 200 PRO Microplate Reader | *The 96-well plate was inserted into an Infinite 200 PRO Microplate Reader | ||
*Then, using the Tecan i-control the proper settings were selected and recorded. | *Then, using the Tecan i-control the proper settings were selected and recorded. | ||
- | **The Settings were: Excitation at 480 (9 nm width) and Emission at 525 (20 nm width) with optimal gain. | + | **The Settings were: Excitation at 480 nm (9 nm width) and Emission at 525 nm (20 nm width) with optimal gain. |
*Finally, the plate reader measured the fluorescence and the absorbance (OD<sub>600</sub>) for each well. | *Finally, the plate reader measured the fluorescence and the absorbance (OD<sub>600</sub>) for each well. | ||
Latest revision as of 03:34, 18 October 2014
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