Team:Austin Texas/interlab study

From 2014.igem.org

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[[File:interlabliquidcultures.jpg|240px|right|thumb| '''Figure 1.''' Triplicate liquid cultures of each construct.]]
[[File:interlabliquidcultures.jpg|240px|right|thumb| '''Figure 1.''' Triplicate liquid cultures of each construct.]]
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<partinfo>I20260 DeepComponents</partinfo>
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'''1.''' The first device is [http://parts.igem.org/Part:BBa_I20260 BBa_I20260], which is a composite BioBrick part that contains:
'''1.''' The first device is [http://parts.igem.org/Part:BBa_I20260 BBa_I20260], which is a composite BioBrick part that contains:
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*The downstream gene is also a composite of a number of BioBrick parts including an RBS - [http://parts.igem.org/Part:BBa_B0032 B0032], a GFP coding sequence - [http://parts.igem.org/Part:BBa_E0040 E0040], and a transcriptional terminator - [http://parts.igem.org/Part:BBa_B0015 B0015].
*The downstream gene is also a composite of a number of BioBrick parts including an RBS - [http://parts.igem.org/Part:BBa_B0032 B0032], a GFP coding sequence - [http://parts.igem.org/Part:BBa_E0040 E0040], and a transcriptional terminator - [http://parts.igem.org/Part:BBa_B0015 B0015].
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<partinfo>J23101 DeepComponents</partinfo>+<partinfo>E0240 DeepComponents</partinfo>
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'''2.''' The second device was cloned in lab, as described in the interlab study procedure.
'''2.''' The second device was cloned in lab, as described in the interlab study procedure.
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*However, the backbone of this device is different, [http://parts.igem.org/Part:pSB3K3 pSB3K3].
*However, the backbone of this device is different, [http://parts.igem.org/Part:pSB3K3 pSB3K3].
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<partinfo>J23115 DeepComponents</partinfo>+<partinfo>E0240 DeepComponents</partinfo>
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'''3.''' The third device was also cloned in lab, as described in the interlab study procedure.
'''3.''' The third device was also cloned in lab, as described in the interlab study procedure.
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*The 96-well plate was inserted into an Infinite 200 PRO Microplate Reader
*The 96-well plate was inserted into an Infinite 200 PRO Microplate Reader
*Then, using the Tecan i-control the proper settings were selected and recorded.
*Then, using the Tecan i-control the proper settings were selected and recorded.
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**The Settings were: Excitation at 480 (9 nm width) and Emission at 525 (20 nm width) with optimal gain.
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**The Settings were: Excitation at 480 nm (9 nm width) and Emission at 525 nm (20 nm width) with optimal gain.
*Finally, the plate reader measured the fluorescence and the absorbance (OD<sub>600</sub>) for each well.
*Finally, the plate reader measured the fluorescence and the absorbance (OD<sub>600</sub>) for each well.

Latest revision as of 03:34, 18 October 2014