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Team:Evry/Overview/Achievements/Projects
From 2014.igem.org
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<FONT color=#003399><h5><b><img src="https://static.igem.org/mediawiki/2014/6/61/Cells_pic.png" width="5%"/>Chassis characterization</b></h5> </font> | <FONT color=#003399><h5><b><img src="https://static.igem.org/mediawiki/2014/6/61/Cells_pic.png" width="5%"/>Chassis characterization</b></h5> </font> | ||
<p><ul style="list-style:none;"> | <p><ul style="list-style:none;"> | ||
| - | <br> <li>- Genome Sequencing</li> | + | <br> <li>- <a href="https://2014.igem.org/Team:Evry/Biology/GenomeAssembly"> Genome Sequencing </a>of <i> Pseudovibrio denitrificans</i> to collect informations defense mechanisms, <br> transposons putative insertion localisation and to possess a assembled genome for RNAseq. </li> |
| - | <br> <li>- Growth conditions optimization </li> | + | <br> <li>- <a href="https://2014.igem.org/Team:Evry/Biology/Chassis">Growth conditions optimization</a> </li> |
| - | <br> <li>- Antibiotic sensitivity </li> | + | <br> <li>- <a href="https://2014.igem.org/Team:Evry/Biology/Chassis"> Antibiotic sensitivity</a> tests shown a potential resistance to tetracycline and ampicilline</li> |
| - | <br> <li>- Electroporation Transformation protocol </li> | + | <br> <li>- <a href="https://2014.igem.org/Team:Evry/Biology/Chassis"> Electroporation Transformation </a> protocol establishment that never been done before </li> |
</ul> | </ul> | ||
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<FONT color=#003399><h5><b><img src="https://static.igem.org/mediawiki/2014/6/6e/Plasmid_khd.png" width="5%"/>New Vector</b></h5> </font> | <FONT color=#003399><h5><b><img src="https://static.igem.org/mediawiki/2014/6/6e/Plasmid_khd.png" width="5%"/>New Vector</b></h5> </font> | ||
<p><ul style="list-style:none;"> | <p><ul style="list-style:none;"> | ||
| - | <br> <li>- Transposon-based vector </li> | + | <br> <li>- <a href="https://2014.igem.org/Team:Evry/Biology/Transposons">Transposon-based vector</a> was created to overcome resistance mechanisms to DNA entrance</li> |
| - | <br> <li>- Proof of concept in <i>Pseudovibrio denitrificans</i> </li> | + | <br> <li>- Proof of concept to use it in <i>Pseudovibrio denitrificans</i> was achieved </li> |
</ul> | </ul> | ||
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<FONT color=#003399><h5><b><img src="https://static.igem.org/mediawiki/2014/9/9c/Sensors.png" width="5%"/>New or Optimized sensors Sensors</b></h5> </font> | <FONT color=#003399><h5><b><img src="https://static.igem.org/mediawiki/2014/9/9c/Sensors.png" width="5%"/>New or Optimized sensors Sensors</b></h5> </font> | ||
<p><ul style="list-style:none;"> | <p><ul style="list-style:none;"> | ||
| - | <br> <li>- <a href="https://2014.igem.org/Team:Evry/Biology/Sensors"> New PCB sensor </a> </li> | + | <br> <li>- <a href="https://2014.igem.org/Team:Evry/Biology/Sensors"> New PCB sensor </a> based on bphR1 promoter and binding protein bphR2.</li> |
| - | <br> <li>- An optimized version of <a href="https://2014.igem.org/Team:Evry/Biology/Sensors"> the Phenol sensors </a></li> | + | <br> <li>- An optimized version of <a href="https://2014.igem.org/Team:Evry/Biology/Sensors"> the Phenol sensors </a> from Pekin iGEM 2013 team was constructed</li> |
| - | <br> <li>- <a href="https://2014.igem.org/Team:Evry/Biology/RNAseq"> RNA-seq data </a> | + | <br> <li>- <a href="https://2014.igem.org/Team:Evry/Biology/RNAseq"> RNA-seq data </a> shall be used to find new promoters</li> |
</ul> | </ul> | ||
</p> | </p> | ||
Latest revision as of 03:56, 18 October 2014
Projects Achievements
Chassis characterization
- - Genome Sequencing of Pseudovibrio denitrificans to collect informations defense mechanisms,
transposons putative insertion localisation and to possess a assembled genome for RNAseq. - - Growth conditions optimization
- - Antibiotic sensitivity tests shown a potential resistance to tetracycline and ampicilline
- - Electroporation Transformation protocol establishment that never been done before
New Vector
- - Transposon-based vector was created to overcome resistance mechanisms to DNA entrance
- - Proof of concept to use it in Pseudovibrio denitrificans was achieved
New or Optimized sensors Sensors
- - New PCB sensor based on bphR1 promoter and binding protein bphR2.
- - An optimized version of the Phenol sensors from Pekin iGEM 2013 team was constructed
- - RNA-seq data shall be used to find new promoters
