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Team:HokkaidoU Japan/Notebook/H Stem/Replacement To 1A3
From 2014.igem.org
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| - | pSB1A3 with EcoRI & PstI | + | pSB1A3 with EcoRI & PstI |
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| + | R0010-stem-as-stem-B0015 with EcoRI & PstI | ||
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| - | 0010-stem-as-stem-B0015 | + | Ligate 0010-stem-as-stem-B0015 with pSB1A3 |
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R0010-stem-as-stem-B0015(on pSB1A3) | R0010-stem-as-stem-B0015(on pSB1A3) | ||
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| + | 5.0µL to DH5alpha; | ||
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Latest revision as of 15:28, 9 September 2015
Notebook
Lab Documents
H-Stem Vector Exchange to pSB1A3
-
Start
-
Mini-Prep
R0010-stem-as-stem-B0015 (on pSB1A3) -
PCR
R0010-stem-as-stem-B0015(on pSB1C3) with 100up_F Tm:62.6℃ & 200dn_R Tm:66.6℃ -
Digestion
pSB1A3 with EcoRI & PstIR0010-stem-as-stem-B0015 with EcoRI & PstI -
Ligation
Ligate 0010-stem-as-stem-B0015 with pSB1A3 -
Transformation
R0010-stem-as-stem-B0015(on pSB1A3)5.0µL to DH5alpha; -
Complete!
