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Team:Groningen/Template/MODULE/Project/secretion/part4
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* It must secrete Aiia when it detect <i> P. aeruginosa</i> | * It must secrete Aiia when it detect <i> P. aeruginosa</i> | ||
* It must secrete Nisin when it detects <i>S. aureus</i> | * It must secrete Nisin when it detects <i>S. aureus</i> | ||
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<html> | <html> | ||
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<!-- PARAGRAPH SNIPPET MANDATORY START--> | <!-- PARAGRAPH SNIPPET MANDATORY START--> | ||
<div class="text"> | <div class="text"> | ||
| - | After designing the systems, | + | After designing the systems, two constructs came out: |
The anti-<i>Pseudomonas aeruginosa</i> system: | The anti-<i>Pseudomonas aeruginosa</i> system: | ||
| + | </html>{{:Team:Groningen/Template/MODULE/newfigure|Figure 6|b/b8/Gen_Construct_2.art.png|The DspB and AiiA construct}}<html> | ||
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| + | The anti-<i>Staphylococcus aureus</i> secretion system: | ||
| - | + | </html>{{:Team:Groningen/Template/MODULE/newfigure|Figure 7|f/f2/Gen_constuct_5.art.png|The DspB and nisin construct}}<html> | |
</div> | </div> | ||
Latest revision as of 03:21, 18 October 2014
The construct
In order to synchronize the secretion of infection preventing molecules with the detection of the certain pathogens, a few conditions must been set.
- The construct should be regulated through detection.
- It must secrete DspB when L. lactis senses both P. aeruginosa and S. aureus
- It must secrete Aiia when it detect P. aeruginosa
- It must secrete Nisin when it detects S. aureus
After designing the systems, two constructs came out:
The anti-Pseudomonas aeruginosa system:
The anti-Staphylococcus aureus secretion system:
Figure 6:
The DspB and AiiA construct
Figure 7:
The DspB and nisin construct
