Team:WashU StLouis/Project/collaboration
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+ | <h1> Collaboration </h1> | ||
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+ | <p> We also collaborated with the University of Virginia's | ||
+ | iGEM Team by helping them distribute their survey! For more | ||
+ | information, contact them at virginia.igem@gmail.com </p> | ||
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+ | } | ||
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+ | <div id="virginiabadge"> <a | ||
+ | href="https://2014.igem.org/Team:Virginia/HumanPractices"><img | ||
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+ | <div style="text-align: center;"><br> | ||
+ | We also helped to characterize a few parts from the registry:<br> | ||
+ | </div> | ||
+ | <p style="text-align: justify;"><a | ||
+ | href="http://parts.igem.org/Part:BBa_M30109:Experience#User_Reviews">BBa_M30109</a> | ||
+ | <br> | ||
+ | BBa_M30109 does NOT work AT ALL. Digesting the plasmid with the iGEM | ||
+ | restriction enzyme yields the right band lengths but other than that | ||
+ | the sequence is junk. We tried on numerous occasions to assemble a | ||
+ | plasmid to activate our light sensor system. This set us back half of | ||
+ | the summer because of the faulty part. We found a new part on the | ||
+ | registry with the same genes that showed slightly more promise. </p> | ||
+ | <p style="text-align: justify;"><a | ||
+ | href="http://parts.igem.org/Part:BBa_K1017726:Experience#User_Reviews">BBa_K1017726</a> | ||
+ | <br> | ||
+ | BBa_K1017726 digests with the iGEM restriction enzymes well. We were | ||
+ | able to assemble a plasmid by swapping out ori and resistance (we | ||
+ | needed a different antibiotic) in order to co-transform with our | ||
+ | system. There is differentiable results between our light and dark | ||
+ | system, so the part likely has the genes as reported. However, | ||
+ | digestion with known restriction enzyme sites based off the sequence | ||
+ | provided gave gel smears rather than clear bands, so not 100% convinced | ||
+ | that this part is as advertised. </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | </tbody> | ||
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Latest revision as of 21:39, 17 October 2014
We also helped to characterize a few parts from the registry: BBa_M30109
BBa_K1017726
|