"
Team:HokkaidoU Japan/Notebook/asB0034/140910 asB0034&B0032 mRFP Assay
From 2014.igem.org
(Difference between revisions)
| (One intermediate revision not shown) | |||
| Line 89: | Line 89: | ||
<li class="ldd_heading"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Outreach/Discussion">Discussion</a></li> | <li class="ldd_heading"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Outreach/Discussion">Discussion</a></li> | ||
<li class="ldd_contents"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Outreach/Discussion#Background">Background</a></li> | <li class="ldd_contents"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Outreach/Discussion#Background">Background</a></li> | ||
| - | <li class="ldd_contents"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Outreach/Discussion# | + | <li class="ldd_contents"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Outreach/Discussion#Evaluation">Evaluation</a></li> |
</ul> | </ul> | ||
</div> | </div> | ||
| Line 250: | Line 250: | ||
<div id="footer-content"> | <div id="footer-content"> | ||
<div id="footer-logo"> | <div id="footer-logo"> | ||
| - | <a href="http://igemhokkaidou.com | + | <a href="http://igemhokkaidou.wordpress.com"><img style="height:150px;position:relative;" src="https://static.igem.org/mediawiki/2014/3/39/HokkaidoU_logo_transparent.png"></a> |
</div> | </div> | ||
<div id="footer-twitter"> | <div id="footer-twitter"> | ||
Latest revision as of 15:32, 9 September 2015
Notebook
Lab Documents
140910 asB0034 & B0032 mRFP Assay
-
Start
-
Double Transformation
Ptrc-stem-asB0034-stem(on pHN1257) & R0040-B0032-E1010-dt(on pSB6A1)each of 2.5µL DNA to DH5α -
Colony PCR
R0040-B0032-E1010-B0015(on pSB6A1) EcoRI_XbaI_F Tm:70.9℃ & RBS_SpeI_R Tm:59.2℃ -
Preculture
Ptrc-stem-asB0034-stem(on pHN1257) & R0040-B0032-E1010-B0015(on pSB6A1)LB 2 mL for 8hoursTurbidity was controlled 0.1 -
Culture
M9ZB 2 mL IPTG 20 µ or not for 9 hours, 11 hours, 13hours -
Assay
fluoresence imageing 514 nm 610 filter -
Complete!
