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Team:Bordeaux/NotebookJuly15
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PCR step 3 : Vtot = 50µL<br> | PCR step 3 : Vtot = 50µL<br> | ||
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Latest revision as of 02:25, 18 October 2014
15th July
Molecular biology
- SLP gene synthesis
PCR step 3 : Vtot = 50µL
10µL phusion buffer 5X
1µL dNTP 10mM
0,5µL phusion polymérase
2,5µL DMSO
2,5µL Primer 1 à 10mM
2,5µL Primer 8 à 10mM
10µL 3° ou 3°°
21µL H2O
PCR Parameter:
98°C 5 min
35 cycles : 98°C 10s, 69°C 20s, 72°C 1min
Final extension 72°C 10min
End at 4°C
Production and purification
Please look our planning of production and purification
